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The map label for this gene is dop [H]

Identifier: 148823323

GI number: 148823323

Start: 2384297

End: 2386132

Strand: Reverse

Name: dop [H]

Synonym: TBFG_12144

Alternate gene names: 148823323

Gene position: 2386132-2384297 (Counterclockwise)

Preceding gene: 148823326

Following gene: 148823322

Centisome position: 53.93

GC content: 68.25

Gene sequence:

>1836_bases
ATGTTCTGGGTCGGCGGGCCTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCGTTG
CTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCGTTGCTTGATGCCCGCCTCCTCAGCGG
CCCGCTGCGCGGCCCGCATCGTCGGCGGGCGTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTC
GGCGGGCGTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCCTAGGCTCTACGGCAT
GCAGCGGATTATCGGAACGGAGGTCGAGTACGGCATTTCCTCGCCGTCGGACCCGACCGCCAACCCGATCCTCACCTCGA
CGCAGGCGGTGCTGGCATACGCCGCCGCCGCCGGCATTCAGCGTGCCAAACGCACCCGTTGGGACTACGAGGTGGAATCG
CCGCTGCGCGACGCCCGGGGCTTCGATTTGAGTCGCTCGGCCGGGCCGCCGCCGGTGGTCGACGCCGACGAGGTCGGCGC
GGCCAACATGATCCTGACCAACGGGGCGCGGCTGTATGTCGACCACGCGCACCCGGAATACTCCGCGCCCGAATGCACCG
ACCCGCTGGACGCAGTGATCTGGGACAAGGCGGGCGAACGCGTGATGGAGGCCGCTGCCCGCCATGTCGCCAGCGTGCCC
GGGGCCGCGAAACTGCAGCTGTACAAGAACAACGTCGACGGCAAGGGAGCCTCCTACGGGTCGCACGAGAACTACCTGAT
GTCGCGGCAGACACCGTTCTCGGCGATCATCACCGGGCTGACCCCCTTTCTGGTATCCCGGCAGGTGGTGACCGGCTCGG
GCCGGGTCGGCATCGGGCCCTCGGGTGATGAGCCCGGCTTCCAGCTATCCCAGCGTTCGGACTACATCGAGGCAGAGGTA
GGGCTGGAAACAACGCTCAAGCGCGGCATCATCAACACCCGCGACGAACCGCACGCCGACGCCGACAGGTACCGCCGGCT
GCACGTCATCATCGGCGACGCCAACCTTGCCGAGACGTCGACCTATCTGAAGTTGGGTACCACGGCGCTGGTGCTCGACC
TGATCGAAGAAGGACCAGCCCACGCAATAGATCTGACCGACCTGGCGCTGGCCCGCCCGGTACATGCGGTGCACGCAATC
TCCCGCGATCCGTCGCTGCGAGCGACCGTTGCGCTGGCCGACGGCCGGGAACTGACCGGTCTTGCGCTGCAACGGATCTA
CCTGGACCGAGTGGCTAAGTTGGTGGATAGCCGCGACCCGGACCCGCGGGCGGCCGACATCGTGGAAACCTGGGCACACG
TGCTGGATCAGCTCGAGCGTGACCCGATGGATTGCGCGGAGCTGCTGGACTGGCCGGCCAAACTGCGGCTGCTCGACGGT
TTCCGGCAGCGGGAGAACCTGAGCTGGTCGGCGCCCCGGCTGCACCTCGTCGACCTGCAGTACTCCGATGTCCGGCTGGA
CAAGGGCCTGTACAACCGGCTGGTCGCGCGCGGCTCGATGAAGCGTTTAGTCACCGAACACCAGGTGCTGAGTGCGGTGG
AGAACCCGCCGACCGACACCCGCGCGTATTTCCGCGGCGAATGCCTGCGCCGGTTCGGGGCTGATATCGCCGCGGCTAGC
TGGGACTCGGTGATCTTCGACCTGGGCGGCGACTCGCTGGTTCGCATCCCGACGCTGGAGCCGTTGCGGGGTAGTAAGGC
GCATGTTGGTGCGTTGCTGGATTCGGTGGACAGTGCCGTGGAGCTGGTAGAGCAACTGACCGCTGAGCCTCGCTAA

Upstream 100 bases:

>100_bases
CTGCTCGGGCCGCCGGGGGGCGACGGACGGAAAGGCTCATGCCGGCCTACCCTAGCGCCGGGCCCTGCTTGATTGTCCAC
CTCCTGCGCGGCCCGCTCCG

Downstream 100 bases:

>100_bases
GCCTGGTTAGGCCGGGAAACGTTGGTGTTGACCGGTAGGGTAGAGAAAGACAGCGGGCGTGTGACGCGCACCCGCGGCCT
TTTGAAAGGCCATGAGGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIV
GGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVES
PLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVP
GAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYIEAEV
GLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAI
SRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDG
FRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADIAAAS
WDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR

Sequences:

>Translated_611_residues
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIV
GGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVES
PLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVP
GAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYIEAEV
GLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAI
SRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDG
FRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADIAAAS
WDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR
>Mature_611_residues
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIV
GGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVES
PLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVP
GAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGPSGDEPGFQLSQRSDYIEAEV
GLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAI
SRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDG
FRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDTRAYFRGECLRRFGADIAAAS
WDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 65719; Mature: 65719

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARI
CCEECCCCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHHCCEEECCCCHHHHHHHHH
VGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGIS
HCCEEECCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCC
SPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHCCCCCCCCCCCCCCC
DADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVP
CCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGP
CCEEEEEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCC
SGDEPGFQLSQRSDYIEAEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETS
CCCCCCCEECCCCCCEEHHCCHHHHHHHCCCCCCCCCCCCHHHCEEEEEEEECCCCHHHH
TYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTG
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
LALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHH
FRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDT
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
RAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAV
HHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
ELVEQLTAEPR
HHHHHHHCCCC
>Mature Secondary Structure
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARI
CCEECCCCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHHCCEEECCCCHHHHHHHHH
VGGRCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGIS
HCCEEECCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCC
SPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHCCCCCCCCCCCCCCC
DADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVASVP
CCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVGIGP
CCEEEEEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCC
SGDEPGFQLSQRSDYIEAEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETS
CCCCCCCEECCCCCCEEHHCCHHHHHHHCCCCCCCCCCCCHHHCEEEEEEEECCCCHHHH
TYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTG
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
LALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRLLDG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHH
FRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPPTDT
HHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
RAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAV
HHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
ELVEQLTAEPR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]