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The map label for this gene is gltD [H]

Identifier: 148663726

GI number: 148663726

Start: 4338485

End: 4339951

Strand: Reverse

Name: gltD [H]

Synonym: MRA_3898

Alternate gene names: 148663726

Gene position: 4339951-4338485 (Counterclockwise)

Preceding gene: 148663727

Following gene: 148663725

Centisome position: 98.19

GC content: 65.17

Gene sequence:

>1467_bases
ATGGCTGATCCGGGCGGCTTCCTCAAATACACCCACCGGAAATTGCCGAAGCGACGGCCGGTCCCGCTGCGGCTGCGAGA
CTGGCGGGAAGTCTACGAGGAATTCGACAACGAGAGCCTGCGCCAACAGGCGACCCGCTGCATGGATTGCGGCATTCCGT
TCTGTCACAACGGATGTCCGCTGGGTAACTTGATCCCGGAATGGAACGATCTGGTCCGCCGGGGCCGCTGGCGCGACGCA
ATCGAACGGCTGCACGCCACCAACAACTTCCCCGACTTCACCGGCCGGTTGTGCCCGGCTCCGTGCGAGCCGGCGTGTGT
GCTGGGCATCAACCAGGATCCGGTGACGATCAAGCAGATCGAGCTGGAGATCATCGACAAGGCCTTCGACGAAGGATGGG
TGCAACCACGTCCGCCGCGGAAGCTGACCGGGCAAACGGTTGCTGTGGTGGGTTCGGGGCCGGCGGGTTTGGCCGCCGCC
CAGCAACTCACCCGGGCGGGTCACACCGTCACCGTTTTCGAGCGCGAAGACCGCATCGGCGGGCTGCTGCGTTACGGCAT
CCCGGAATTCAAGATGGAAAAGCGGCATCTTGATCGGCGTTTGGACCAAATGCGCTCCGAAGGAACCGAATTCCGGCCGG
GCGTCAACGTCGGGGTCGACATTAGCGCCGAAAAGCTGCGCGCTGATTTCGATGCGGTCGTATTGGCCGGTGGCGCAACC
GCTTGGCGCGAGCTGCCGATTCCCGGCCGCGAGCTGGAGGGCGTCCATCAGGCGATGGAGTTTCTGCCGTGGGCCAACCG
GGTGCAAGAAGGAGATGACGTCCTCGATGAGGACGGGCAGCCGCCGATCACCGCAAAGGGCAAGAAGGTCGTCATCATCG
GTGGCGGCGACACCGGGGCGGACTGCCTGGGTACCGTGCACCGCCAGGGCGCGATCGCCGTGCACCAGTTCGAGATCATG
CCGCGTCCACCGGACGCCCGCGCCGAATCCACCCCATGGCCGACTTACCCGCTGATGTATCGGGTATCGGCCGCGCATGA
GGAGGGTGGCGAGCGGGTGTTCTCGGTCAACACCGAGGCGTTCGTCGGCACGGACGGGCGCGTGAGCGCGCTGCGGGCAC
ACGAAGTGACCATGCTGGATGGCAAGTTCGTCAAGGTTGAGGGCTCCGACTTCGAGCTCGAAGCCGACCTGGTGTTGCTG
GCGATGGGTTTCGTCGGCCCGGAACGGGCGGGCCTGTTGACCGACCTCGGAGTGAAGTTCACCGAGCGTGGGAACGTGGC
CCGTGGCGACGACTTCGACACCTCGGTTCCTGGTGTGTTCGTAGCCGGTGACATGGGCCGGGGCCAGTCATTGATCGTCT
GGGCGATTGCCGAGGGCCGGGCCGCTGCCGCGGCCGTAGACCGGTACTTGATGGGTTCCAGTGCCCTGCCGGCGCCTGTT
AAGCCGACCGCTGCACCGCTTCAATAA

Upstream 100 bases:

>100_bases
GTCAAGGTGATGCCGCGTGACTACAAACGGGTCCTGCAGGCGATCGCCCTGGCTGAACGTGACGGCGTTGATGTCGACAA
GGCGATCATGGCGGCTGCGC

Downstream 100 bases:

>100_bases
TCACTAGAGTCACATCAGAAACATTTGATAATTGCTCGGCGCGCCGTTCACTGTTTGCCAGCTCCGAGGTGTCTAATGAG
TCGATGTGGGCTTCGTCACA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MADPGGFLKYTHRKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWRDA
IERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQIELEIIDKAFDEGWVQPRPPRKLTGQTVAVVGSGPAGLAAA
QQLTRAGHTVTVFEREDRIGGLLRYGIPEFKMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGAT
AWRELPIPGRELEGVHQAMEFLPWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGADCLGTVHRQGAIAVHQFEIM
PRPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEAFVGTDGRVSALRAHEVTMLDGKFVKVEGSDFELEADLVLL
AMGFVGPERAGLLTDLGVKFTERGNVARGDDFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYLMGSSALPAPV
KPTAAPLQ

Sequences:

>Translated_488_residues
MADPGGFLKYTHRKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWRDA
IERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQIELEIIDKAFDEGWVQPRPPRKLTGQTVAVVGSGPAGLAAA
QQLTRAGHTVTVFEREDRIGGLLRYGIPEFKMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGAT
AWRELPIPGRELEGVHQAMEFLPWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGADCLGTVHRQGAIAVHQFEIM
PRPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEAFVGTDGRVSALRAHEVTMLDGKFVKVEGSDFELEADLVLL
AMGFVGPERAGLLTDLGVKFTERGNVARGDDFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYLMGSSALPAPV
KPTAAPLQ
>Mature_487_residues
ADPGGFLKYTHRKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCPLGNLIPEWNDLVRRGRWRDAI
ERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQIELEIIDKAFDEGWVQPRPPRKLTGQTVAVVGSGPAGLAAAQ
QLTRAGHTVTVFEREDRIGGLLRYGIPEFKMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGATA
WRELPIPGRELEGVHQAMEFLPWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGADCLGTVHRQGAIAVHQFEIMP
RPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEAFVGTDGRVSALRAHEVTMLDGKFVKVEGSDFELEADLVLLA
MGFVGPERAGLLTDLGVKFTERGNVARGDDFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYLMGSSALPAPVK
PTAAPLQ

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=469, Percent_Identity=23.0277185501066, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI111118983, Length=157, Percent_Identity=33.7579617834395, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI111118981, Length=157, Percent_Identity=33.7579617834395, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789606, Length=475, Percent_Identity=37.0526315789474, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1788811, Length=472, Percent_Identity=34.7457627118644, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87082180, Length=481, Percent_Identity=34.0956340956341, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788468, Length=438, Percent_Identity=27.6255707762557, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789243, Length=429, Percent_Identity=27.972027972028, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=503, Percent_Identity=45.9244532803181, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17543860, Length=168, Percent_Identity=35.7142857142857, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=492, Percent_Identity=46.5447154471545, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320584, Length=176, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574881, Length=493, Percent_Identity=49.8985801217039, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24665539, Length=493, Percent_Identity=49.8985801217039, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24665547, Length=493, Percent_Identity=49.8985801217039, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24665543, Length=493, Percent_Identity=49.8985801217039, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24640763, Length=471, Percent_Identity=24.6284501061571, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI18858217, Length=471, Percent_Identity=24.6284501061571, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53452; Mature: 53321

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPGGFLKYTHRKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCP
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC
LGNLIPEWNDLVRRGRWRDAIERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQI
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEH
ELEIIDKAFDEGWVQPRPPRKLTGQTVAVVGSGPAGLAAAQQLTRAGHTVTVFEREDRIG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GLLRYGIPEFKMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGAT
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCEEEEECCCH
AWRELPIPGRELEGVHQAMEFLPWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGA
HHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCH
DCLGTVHRQGAIAVHQFEIMPRPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEA
HHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCEEEEECCCE
FVGTDGRVSALRAHEVTMLDGKFVKVEGSDFELEADLVLLAMGFVGPERAGLLTDLGVKF
EECCCCCEEEEEEEEEEEECCEEEEEECCCCEEECCEEEEEHHCCCCCCCCCHHHCCCEE
TERGNVARGDDFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYLMGSSALPAPV
ECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCC
KPTAAPLQ
CCCCCCCC
>Mature Secondary Structure 
ADPGGFLKYTHRKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCP
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC
LGNLIPEWNDLVRRGRWRDAIERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQI
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEH
ELEIIDKAFDEGWVQPRPPRKLTGQTVAVVGSGPAGLAAAQQLTRAGHTVTVFEREDRIG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GLLRYGIPEFKMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGAT
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCEEEEECCCH
AWRELPIPGRELEGVHQAMEFLPWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGA
HHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCH
DCLGTVHRQGAIAVHQFEIMPRPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEA
HHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCEEEEECCCE
FVGTDGRVSALRAHEVTMLDGKFVKVEGSDFELEADLVLLAMGFVGPERAGLLTDLGVKF
EECCCCCEEEEEEEEEEEECCEEEEEECCCCEEECCEEEEEHHCCCCCCCCCHHHCCCEE
TERGNVARGDDFDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYLMGSSALPAPV
ECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCC
KPTAAPLQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]