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The map label for this gene is serS

Identifier: 148663702

GI number: 148663702

Start: 4316101

End: 4317360

Strand: Reverse

Name: serS

Synonym: MRA_3874

Alternate gene names: 148663702

Gene position: 4317360-4316101 (Counterclockwise)

Preceding gene: 148663705

Following gene: 148663700

Centisome position: 97.68

GC content: 67.78

Gene sequence:

>1260_bases
GTGATCGACCTGAAGCTGCTTCGTGAAAACCCCGACGCGGTACGCCGCTCACAACTCAGCCGCGGCGAGGACCCGGCGCT
GGTAGATGCCCTGCTGACGGCCGACGCCGCCCGCCGGGCCGTGATCTCGACCGCCGATTCGTTACGGGCCGAGCAGAAAG
CCGCCAGCAAAAGCGTGGGTGGCGCGTCTCCCGAAGAGCGCCCGCCGCTGCTGCGGCGCGCGAAGGAACTCGCCGAGCAG
GTCAAAGCCGCTGAGGCCGACGAGGTCGAAGCGGAGGCGGCGTTCACCGCGGCGCACCTGGCGATCTCGAATGTCATCGT
GGACGGGGTACCCGCCGGCGGGGAGGACGACTACGCGGTGCTCGACGTCGTCGGCGAGCCCAGCTACCTCGAGAACCCCA
AGGACCACCTGGAGCTCGGCGAGTCGCTGGGCCTGATCGACATGCAGCGCGGCGCCAAGGTGTCGGGTTCACGGTTCTAC
TTCCTGACCGGTCGGGGTGCCCTACTGCAGCTTGGATTGCTGCAGCTGGCGCTGAAGCTAGCCGTCGACAACGGCTTTGT
CCCTACGATCCCGCCGGTGCTGGTGCGCCCGGAAGTGATGGTAGGCACGGGATTTCTAGGCGCCCACGCCGAGGAGGTGT
ACCGGGTAGAGGGCGACGGCCTCTACCTTGTGGGCACCTCCGAGGTACCGCTGGCGGGGTATCACTCCGGCGAGATTCTG
GACCTTTCCCGCGGGCCGCTGCGGTATGCGGGCTGGTCGTCGTGTTTCCGACGTGAGGCCGGCAGCCATGGCAAGGACAC
GCGCGGCATCATCCGGGTGCACCAGTTCGACAAAGTCGAGGGCTTCGTCTACTGCACACCGGCCGACGCGGAGCACGAAC
ATGAGCGGCTGCTGGGCTGGCAGCGCCAGATGCTGGCACGCATCGAGGTGCCGTATCGGGTCATCGACGTGGCCGCGGGT
GATCTCGGCTCGTCGGCCGCCCGCAAGTTCGACTGCGAGGCGTGGATTCCGACGCAGGGGGCCTATCGCGAGCTGACGTC
GACGTCGAACTGCACCACCTTTCAGGCGCGCCGGTTGGCGACCCGCTACCGGGATGCCAGCGGCAAGCCGCAGATCGCGG
CCACCCTCAACGGAACGCTGGCCACCACCCGGTGGCTGGTTGCGATCCTGGAGAACCACCAGCGGCCCGACGGCAGCGTT
AGAGTCCCGGACGCACTGGTTCCGTTCGTGGGTGTCGAAGTGCTGGAGCCGGTCGCTTAG

Upstream 100 bases:

>100_bases
ATGACCAATGTTGCAGTTGTCAGCTTGCGGGATCGCTTCATCGCGGGTGGATGGTCCTCTGCCGCCACCCTGACTGGGCG
TCAAAAGTAGTCTTGCGGCC

Downstream 100 bases:

>100_bases
CGGCGATCGCGAGCGCGGCGGAGCCGGGTGAGGCGGGTCGCCGCCATTGGCTTAGCGGCTTGGCCGGGAGTGTGTCCGAA
GGCTTGTCGGTAGGTGTCAA

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MIDLKLLRENPDAVRRSQLSRGEDPALVDALLTADAARRAVISTADSLRAEQKAASKSVGGASPEERPPLLRRAKELAEQ
VKAAEADEVEAEAAFTAAHLAISNVIVDGVPAGGEDDYAVLDVVGEPSYLENPKDHLELGESLGLIDMQRGAKVSGSRFY
FLTGRGALLQLGLLQLALKLAVDNGFVPTIPPVLVRPEVMVGTGFLGAHAEEVYRVEGDGLYLVGTSEVPLAGYHSGEIL
DLSRGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQFDKVEGFVYCTPADAEHEHERLLGWQRQMLARIEVPYRVIDVAAG
DLGSSAARKFDCEAWIPTQGAYRELTSTSNCTTFQARRLATRYRDASGKPQIAATLNGTLATTRWLVAILENHQRPDGSV
RVPDALVPFVGVEVLEPVA

Sequences:

>Translated_419_residues
MIDLKLLRENPDAVRRSQLSRGEDPALVDALLTADAARRAVISTADSLRAEQKAASKSVGGASPEERPPLLRRAKELAEQ
VKAAEADEVEAEAAFTAAHLAISNVIVDGVPAGGEDDYAVLDVVGEPSYLENPKDHLELGESLGLIDMQRGAKVSGSRFY
FLTGRGALLQLGLLQLALKLAVDNGFVPTIPPVLVRPEVMVGTGFLGAHAEEVYRVEGDGLYLVGTSEVPLAGYHSGEIL
DLSRGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQFDKVEGFVYCTPADAEHEHERLLGWQRQMLARIEVPYRVIDVAAG
DLGSSAARKFDCEAWIPTQGAYRELTSTSNCTTFQARRLATRYRDASGKPQIAATLNGTLATTRWLVAILENHQRPDGSV
RVPDALVPFVGVEVLEPVA
>Mature_419_residues
MIDLKLLRENPDAVRRSQLSRGEDPALVDALLTADAARRAVISTADSLRAEQKAASKSVGGASPEERPPLLRRAKELAEQ
VKAAEADEVEAEAAFTAAHLAISNVIVDGVPAGGEDDYAVLDVVGEPSYLENPKDHLELGESLGLIDMQRGAKVSGSRFY
FLTGRGALLQLGLLQLALKLAVDNGFVPTIPPVLVRPEVMVGTGFLGAHAEEVYRVEGDGLYLVGTSEVPLAGYHSGEIL
DLSRGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQFDKVEGFVYCTPADAEHEHERLLGWQRQMLARIEVPYRVIDVAAG
DLGSSAARKFDCEAWIPTQGAYRELTSTSNCTTFQARRLATRYRDASGKPQIAATLNGTLATTRWLVAILENHQRPDGSV
RVPDALVPFVGVEVLEPVA

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=470, Percent_Identity=33.6170212765957, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI8923421, Length=385, Percent_Identity=35.8441558441558, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI225579074, Length=334, Percent_Identity=38.622754491018, Blast_Score=216, Evalue=5e-56,
Organism=Escherichia coli, GI1787120, Length=430, Percent_Identity=36.7441860465116, Blast_Score=243, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17539260, Length=461, Percent_Identity=34.2733188720174, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17542084, Length=326, Percent_Identity=34.0490797546012, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320226, Length=440, Percent_Identity=32.5, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321799, Length=284, Percent_Identity=37.3239436619718, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24581334, Length=461, Percent_Identity=34.2733188720174, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI17737985, Length=285, Percent_Identity=36.4912280701754, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_MYCBO (P67562)

Other databases:

- EMBL:   BX248347
- RefSeq:   NP_857501.1
- ProteinModelPortal:   P67562
- SMR:   P67562
- EnsemblBacteria:   EBMYCT00000015118
- GeneID:   1093850
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3864c
- GeneTree:   EBGT00050000016582
- HOGENOM:   HBG629391
- OMA:   PKFDFKP
- ProtClustDB:   PRK05431
- BioCyc:   MBOV233413:MB3864C-MONOMER
- BRENDA:   6.1.1.11
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 45325; Mature: 45325

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 273-273 BINDING 280-280 BINDING 379-379

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLKLLRENPDAVRRSQLSRGEDPALVDALLTADAARRAVISTADSLRAEQKAASKSVG
CCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GASPEERPPLLRRAKELAEQVKAAEADEVEAEAAFTAAHLAISNVIVDGVPAGGEDDYAV
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEE
LDVVGEPSYLENPKDHLELGESLGLIDMQRGAKVSGSRFYFLTGRGALLQLGLLQLALKL
EEEECCCHHHCCCHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
AVDNGFVPTIPPVLVRPEVMVGTGFLGAHAEEVYRVEGDGLYLVGTSEVPLAGYHSGEIL
HHCCCCCCCCCCHHCCCCEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCEE
DLSRGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQFDKVEGFVYCTPADAEHEHERLLGW
ECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHH
QRQMLARIEVPYRVIDVAAGDLGSSAARKFDCEAWIPTQGAYRELTSTSNCTTFQARRLA
HHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCEECCCCCHHHHHCCCCCCCHHHHHHHH
TRYRDASGKPQIAATLNGTLATTRWLVAILENHQRPDGSVRVPDALVPFVGVEVLEPVA
HHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDLKLLRENPDAVRRSQLSRGEDPALVDALLTADAARRAVISTADSLRAEQKAASKSVG
CCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GASPEERPPLLRRAKELAEQVKAAEADEVEAEAAFTAAHLAISNVIVDGVPAGGEDDYAV
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEE
LDVVGEPSYLENPKDHLELGESLGLIDMQRGAKVSGSRFYFLTGRGALLQLGLLQLALKL
EEEECCCHHHCCCHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
AVDNGFVPTIPPVLVRPEVMVGTGFLGAHAEEVYRVEGDGLYLVGTSEVPLAGYHSGEIL
HHCCCCCCCCCCHHCCCCEEEECCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCEE
DLSRGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQFDKVEGFVYCTPADAEHEHERLLGW
ECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHH
QRQMLARIEVPYRVIDVAAGDLGSSAARKFDCEAWIPTQGAYRELTSTSNCTTFQARRLA
HHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCEECCCCCHHHHHCCCCCCCHHHHHHHH
TRYRDASGKPQIAATLNGTLATTRWLVAILENHQRPDGSVRVPDALVPFVGVEVLEPVA
HHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972