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The map label for this gene is fadD32 [H]

Identifier: 148663669

GI number: 148663669

Start: 4269599

End: 4271512

Strand: Reverse

Name: fadD32 [H]

Synonym: MRA_3841

Alternate gene names: 148663669

Gene position: 4271512-4269599 (Counterclockwise)

Preceding gene: 148663670

Following gene: 148663668

Centisome position: 96.64

GC content: 65.46

Gene sequence:

>1914_bases
ATGTTTGTGACAGGAGAGAGTGGGATGGCGTACCACAACCCGTTCATCGTGAATGGAAAGATCAGGTTCCCAGCCAACAC
CAACCTGGTTCGTCACGTCGAAAAGTGGGCGAAGGTTCGTGGCGACAAGCTGGCCTACCGGTTCCTGGACTTTTCCACCG
AACGAGACGGTGTCGCGCGCGACATCTTGTGGTCTGACTTCAGCGCGCGCAACCGTGCGGTGGGAGCCCGCCTGCAGCAA
GTCACCCAGCCGGGTGACCGCGTCGCCATCCTGTGCCCGCAGAACCTGGACTACCTCATCTCCTTCTTCGGCGCCCTCTA
CTCCGGCCGCATTGCGGTGCCGTTGTTCGACCCGGCCGAGCCGGGGCACGTCGGTCGGTTACACGCGGTGCTCGACGACT
GTGCCCCGTCGACGATCCTGACCACCACCGACTCCGCCGAAGGGGTCCGCAAGTTCATCCGGGCCCGATCGGCCAAGGAG
CGCCCGCGCGTCATTGCCGTCGACGCGGTGCCCACCGAAGTCGCCGCCACCTGGCAGCAGCCCGAGGCCAACGAGGAAAC
CGTCGCGTACTTGCAGTACACGTCGGGTTCCACCCGCATACCGAGCGGCGTGCAGATCACCCATCTGAACCTGCCCACCA
ATGTGGTGCAGGTGCTCAATGCCCTGGAAGGACAGGAAGGCGACCGCGGGGTCAGCTGGCTCCCGTTCTTCCACGACATG
GGTCTGATCACGGTGCTGCTGGCGTCGGTGCTAGGCCACAGCTTCACCTTTATGACGCCCGCGGCGTTCGTGCGGCGGCC
CGGTCGCTGGATCCGCGAGCTCGCCCGCAAGCCCGGAGAAACCGGTGGCACCTTCTCTGCGGCGCCGAACTTCGCATTCG
AACACGCCGCGGTGCGCGGTGTGCCTCGAGACGACGAGCCGCCGCTGGACCTGAGCAACGTCAAGGGCATCCTCAACGGC
AGCGAGCCGGTGTCGCCGGCGTCGATGCGCAAGTTCTTCGAAGCATTTGCGCCGTACGGTTTGAAGCAGACCGCCGTCAA
GCCGTCCTATGGGCTAGCGGAGGCCACGCTGTTCGTCTCGACCACGCCGATGGACGAGGTGCCCACCGTGATCCACGTGG
ACCGCGACGAGCTGAACAACCAGCGGTTCGTTGAGGTGGCCGCCGATGCACCCAACGCCGTCGCCCAGGTCTCTGCGGGC
AAGGTCGGGGTCAGCGAATGGGCGGTCATCGTCGACGCCGACACGGCCAGCGAACTGCCGGACGGACAGATCGGTGAGAT
CTGGCTGCACGGCAACAACTTGGGTACCGGTTATTGGGGCAAAGAAGAAGAGTCCGCCCAGACCTTCAAGAACATCCTCA
AATCGCGGATCAGCGAGTCGCGCGCCGAGGGCGCCCCAGACGACGCGCTGTGGGTGCGTACCGGCGACTACGGCACCTAC
TTCAAGGACCACCTCTACATAGCCGGCCGGATCAAGGACCTCGTCATCATCGACGGCCGCAATCACTACCCACAGGATCT
CGAGTGCACGGCGCAGGAGTCGACCAAGGCGTTGCGGGTTGGCTACGCGGCGGCCTTCTCGGTTCCGGCCAACCAGCTTC
CTCAGACAGTGTTCGACGACTCGCACGCCGGGCTGAAATTCGACCCCGAGGACACCTCCGAGCAGCTGGTGATCGTCGGC
GAACGGGCGGCCGGCACGCATAAGCTCGACCACCAGCCCATCGTCGATGACATCCGGGCGGCCATCGCCGTCGGGCATGG
GGTGACCGTGCGTGACGTCCTGCTGGTGTCGGCCGGCACGATTCCGCGAACCTCCAGCGGCAAGATCGGCCGCCGCGCCT
GCCGTGCGGCCTACCTCGACGGCAGCCTGCGCAGTGGCGTGGGTTCCCCGACGGTCTTCGCCACTTCGGACTGA

Upstream 100 bases:

>100_bases
GCAAACTGCGTTCGCCGACGTGAGCGCGGTAGGGGTTGATGTGCGCGGGTAACCCGTTGGCGGAGCCGACACCCGGTGGC
CGCCGGCAGAGCCGGCCGAG

Downstream 100 bases:

>100_bases
GGACCCATGGCTGACGTAGCGGAATCCCAGGAGAACGCCCCCGCCGAAAGGGCCGAGCTAACGGTCCCCGAGATGCGCCA
GTGGCTGCGCAACTGGGTGG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MFVTGESGMAYHNPFIVNGKIRFPANTNLVRHVEKWAKVRGDKLAYRFLDFSTERDGVARDILWSDFSARNRAVGARLQQ
VTQPGDRVAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPGHVGRLHAVLDDCAPSTILTTTDSAEGVRKFIRARSAKE
RPRVIAVDAVPTEVAATWQQPEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVVQVLNALEGQEGDRGVSWLPFFHDM
GLITVLLASVLGHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAVRGVPRDDEPPLDLSNVKGILNG
SEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTTPMDEVPTVIHVDRDELNNQRFVEVAADAPNAVAQVSAG
KVGVSEWAVIVDADTASELPDGQIGEIWLHGNNLGTGYWGKEEESAQTFKNILKSRISESRAEGAPDDALWVRTGDYGTY
FKDHLYIAGRIKDLVIIDGRNHYPQDLECTAQESTKALRVGYAAAFSVPANQLPQTVFDDSHAGLKFDPEDTSEQLVIVG
ERAAGTHKLDHQPIVDDIRAAIAVGHGVTVRDVLLVSAGTIPRTSSGKIGRRACRAAYLDGSLRSGVGSPTVFATSD

Sequences:

>Translated_637_residues
MFVTGESGMAYHNPFIVNGKIRFPANTNLVRHVEKWAKVRGDKLAYRFLDFSTERDGVARDILWSDFSARNRAVGARLQQ
VTQPGDRVAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPGHVGRLHAVLDDCAPSTILTTTDSAEGVRKFIRARSAKE
RPRVIAVDAVPTEVAATWQQPEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVVQVLNALEGQEGDRGVSWLPFFHDM
GLITVLLASVLGHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAVRGVPRDDEPPLDLSNVKGILNG
SEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTTPMDEVPTVIHVDRDELNNQRFVEVAADAPNAVAQVSAG
KVGVSEWAVIVDADTASELPDGQIGEIWLHGNNLGTGYWGKEEESAQTFKNILKSRISESRAEGAPDDALWVRTGDYGTY
FKDHLYIAGRIKDLVIIDGRNHYPQDLECTAQESTKALRVGYAAAFSVPANQLPQTVFDDSHAGLKFDPEDTSEQLVIVG
ERAAGTHKLDHQPIVDDIRAAIAVGHGVTVRDVLLVSAGTIPRTSSGKIGRRACRAAYLDGSLRSGVGSPTVFATSD
>Mature_637_residues
MFVTGESGMAYHNPFIVNGKIRFPANTNLVRHVEKWAKVRGDKLAYRFLDFSTERDGVARDILWSDFSARNRAVGARLQQ
VTQPGDRVAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPGHVGRLHAVLDDCAPSTILTTTDSAEGVRKFIRARSAKE
RPRVIAVDAVPTEVAATWQQPEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVVQVLNALEGQEGDRGVSWLPFFHDM
GLITVLLASVLGHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAVRGVPRDDEPPLDLSNVKGILNG
SEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTTPMDEVPTVIHVDRDELNNQRFVEVAADAPNAVAQVSAG
KVGVSEWAVIVDADTASELPDGQIGEIWLHGNNLGTGYWGKEEESAQTFKNILKSRISESRAEGAPDDALWVRTGDYGTY
FKDHLYIAGRIKDLVIIDGRNHYPQDLECTAQESTKALRVGYAAAFSVPANQLPQTVFDDSHAGLKFDPEDTSEQLVIVG
ERAAGTHKLDHQPIVDDIRAAIAVGHGVTVRDVLLVSAGTIPRTSSGKIGRRACRAAYLDGSLRSGVGSPTVFATSD

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=523, Percent_Identity=23.3269598470363, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI55749758, Length=589, Percent_Identity=24.2784380305603, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI225735629, Length=461, Percent_Identity=21.9088937093275, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI45827692, Length=461, Percent_Identity=21.9088937093275, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1788107, Length=594, Percent_Identity=26.2626262626263, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI193202679, Length=581, Percent_Identity=21.342512908778, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654656, Length=485, Percent_Identity=23.2989690721649, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 69233; Mature: 69233

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTGESGMAYHNPFIVNGKIRFPANTNLVRHVEKWAKVRGDKLAYRFLDFSTERDGVAR
CEEECCCCCEECCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
DILWSDFSARNRAVGARLQQVTQPGDRVAILCPQNLDYLISFFGALYSGRIAVPLFDPAE
HHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCC
PGHVGRLHAVLDDCAPSTILTTTDSAEGVRKFIRARSAKERPRVIAVDAVPTEVAATWQQ
CCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCC
PEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVVQVLNALEGQEGDRGVSWLPFFHDM
CCCCCCEEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEECHHHHHH
GLITVLLASVLGHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAVRG
HHHHHHHHHHHCCCEECCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
VPRDDEPPLDLSNVKGILNGSEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVS
CCCCCCCCCCHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEEEEE
TTPMDEVPTVIHVDRDELNNQRFVEVAADAPNAVAQVSAGKVGVSEWAVIVDADTASELP
ECCCCCCCCEEEECHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCC
DGQIGEIWLHGNNLGTGYWGKEEESAQTFKNILKSRISESRAEGAPDDALWVRTGDYGTY
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCE
FKDHLYIAGRIKDLVIIDGRNHYPQDLECTAQESTKALRVGYAAAFSVPANQLPQTVFDD
EECEEEEEEEEEEEEEECCCCCCCCCCEECCHHCCHHHHEEEHEEECCCHHHCCHHHCCC
SHAGLKFDPEDTSEQLVIVGERAAGTHKLDHQPIVDDIRAAIAVGHGVTVRDVLLVSAGT
CCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHEEEEECCC
IPRTSSGKIGRRACRAAYLDGSLRSGVGSPTVFATSD
CCCCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECC
>Mature Secondary Structure
MFVTGESGMAYHNPFIVNGKIRFPANTNLVRHVEKWAKVRGDKLAYRFLDFSTERDGVAR
CEEECCCCCEECCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
DILWSDFSARNRAVGARLQQVTQPGDRVAILCPQNLDYLISFFGALYSGRIAVPLFDPAE
HHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCC
PGHVGRLHAVLDDCAPSTILTTTDSAEGVRKFIRARSAKERPRVIAVDAVPTEVAATWQQ
CCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCC
PEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVVQVLNALEGQEGDRGVSWLPFFHDM
CCCCCCEEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEECHHHHHH
GLITVLLASVLGHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAVRG
HHHHHHHHHHHCCCEECCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCC
VPRDDEPPLDLSNVKGILNGSEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVS
CCCCCCCCCCHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEEEEE
TTPMDEVPTVIHVDRDELNNQRFVEVAADAPNAVAQVSAGKVGVSEWAVIVDADTASELP
ECCCCCCCCEEEECHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCC
DGQIGEIWLHGNNLGTGYWGKEEESAQTFKNILKSRISESRAEGAPDDALWVRTGDYGTY
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCE
FKDHLYIAGRIKDLVIIDGRNHYPQDLECTAQESTKALRVGYAAAFSVPANQLPQTVFDD
EECEEEEEEEEEEEEEECCCCCCCCCCEECCHHCCHHHHEEEHEEECCCHHHCCHHHCCC
SHAGLKFDPEDTSEQLVIVGERAAGTHKLDHQPIVDDIRAAIAVGHGVTVRDVLLVSAGT
CCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHEEEEECCC
IPRTSSGKIGRRACRAAYLDGSLRSGVGSPTVFATSD
CCCCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]