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The map label for this gene is glpK

Identifier: 148663559

GI number: 148663559

Start: 4146648

End: 4148201

Strand: Reverse

Name: glpK

Synonym: MRA_3731

Alternate gene names: 148663559

Gene position: 4148201-4146648 (Counterclockwise)

Preceding gene: 148663560

Following gene: 148663557

Centisome position: 93.85

GC content: 64.29

Gene sequence:

>1554_bases
GTGTCCGACGCCATCCTAGGAGAGCAATTGGCCGAGTCCTCGGATTTCATAGCCGCCATCGACCAGGGCACCACCAGCAC
CCGCTGCATGATCTTCGATCACCACGGTGCCGAGGTGGCCCGCCACCAGCTCGAGCACGAGCAGATCCTGCCCCGGGCCG
GCTGGGTGGAGCACAACCCGGTCGAGATCTGGGAGCGCACCGCGTCGGTGTTGATCTCGGTGCTCAACGCCACCAACCTA
TCGCCGAAAGATATTGCCGCGTTGGGGATTACCAACCAACGTGAGACGACGCTGGTATGGAATCGGCACACCGGACGGCC
CTACTACAACGCGATTGTATGGCAGGATACCCGCACCGACCGCATCGCGTCGGCGCTGGATCGAGACGGTCGTGGAAACC
TGATCCGCCGCAAGGCGGGCCTGCCGCCGGCAACTTATTTCTCTGGCGGCAAGCTGCAGTGGATCCTGGAAAATGTCGAT
GGAGTCCGCGCGGCCGCCGAGAACGGCGACGCATTGTTCGGCACACCGGACACCTGGGTGTTGTGGAATCTGACCGGCGG
GCCGCGGGGGGGTGTGCATGTCACCGATGTAACCAACGCCAGCCGGACCATGTTGATGGATCTAGAGACGCTGGACTGGG
ACGACGAGCTGTTGTCGTTGTTTTCGATACCTCGGGCCATGCTGCCCGAGATCGCATCGTCGGCGCCGTCGGAGCCTTAC
GGTGTCACGCTGGCGACCGGGCCTGTCGGCGGTGAGGTGCCGATCACCGGAGTTCTCGGTGATCAGCATGCGGCCATGGT
CGGTCAAGTCTGTCTGGCCCCAGGGGAGGCGAAAAACACCTATGGGACCGGCAATTTTCTGCTGCTGAACACCGGTGAAA
CGATCGTGCGATCGAATAACGGCCTGCTAACCACGGTGTGCTACCAATTCGGGAACGCTAAACCCGTGTACGCGCTTGAA
GGTTCGATCGCGGTGACCGGCTCGGCGGTGCAGTGGCTACGCGATCAGCTGGGCATCATCAGCGGCGCCGCACAGAGTGA
GGCGCTGGCCCGCCAGGTCCCCGACAACGGCGGCATGTATTTCGTGCCGGCGTTTTCCGGGCTGTTCGCGCCATACTGGC
GGTCCGATGCGCGCGGCGCGATCGTCGGGTTGTCGCGGTTCAACACCAACGCGCACCTGGCGCGCGCAACGCTGGAGGCG
ATCTGCTACCAGAGCCGCGATGTGGTGGACGCCATGGAAGCAGACTCCGGTGTTCGCCTGCAGGTGTTGAAGGTGGATGG
CGGGATCACCGGCAACGACCTGTGTATGCAGATCCAGGCCGACGTGTTGGGTGTGGATGTGGTGCGGCCGGTGGTCGCCG
AGACCACCGCACTAGGTGTGGCCTACGCGGCGGGCTTGGCGGTCGGGTTCTGGGCGGCTCCGTCCGATCTGCGGGCCAAC
TGGCGAGAGGACAAGCGGTGGACACCGACGTGGGACGACGACGAGCGTGCCGCGGGTTATGCCGGCTGGCGCAAGGCGGT
GCAGCGGACCCTGGATTGGGTTGACGTGTCCTAG

Upstream 100 bases:

>100_bases
GACTTCCGCAAGTTCGAGGGCATTAGAATTCGCGACCCCTTCTCCGGCTGACGGCGTCGGACCCGCCGCTGCGGTGGACC
ATCATGGACGATTACATGCA

Downstream 100 bases:

>100_bases
CTTTCGCTGTGCGCCTGAACATGTCCGCACGCTCGGGGGATCCGGCGCAGCGAAGACTAGCTGCTAGCCCGGCGGCGAGA
ATCCCGGGCCGTCCGGCGCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSDAILGEQLAESSDFIAAIDQGTTSTRCMIFDHHGAEVARHQLEHEQILPRAGWVEHNPVEIWERTASVLISVLNATNL
SPKDIAALGITNQRETTLVWNRHTGRPYYNAIVWQDTRTDRIASALDRDGRGNLIRRKAGLPPATYFSGGKLQWILENVD
GVRAAAENGDALFGTPDTWVLWNLTGGPRGGVHVTDVTNASRTMLMDLETLDWDDELLSLFSIPRAMLPEIASSAPSEPY
GVTLATGPVGGEVPITGVLGDQHAAMVGQVCLAPGEAKNTYGTGNFLLLNTGETIVRSNNGLLTTVCYQFGNAKPVYALE
GSIAVTGSAVQWLRDQLGIISGAAQSEALARQVPDNGGMYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEA
ICYQSRDVVDAMEADSGVRLQVLKVDGGITGNDLCMQIQADVLGVDVVRPVVAETTALGVAYAAGLAVGFWAAPSDLRAN
WREDKRWTPTWDDDERAAGYAGWRKAVQRTLDWVDVS

Sequences:

>Translated_517_residues
MSDAILGEQLAESSDFIAAIDQGTTSTRCMIFDHHGAEVARHQLEHEQILPRAGWVEHNPVEIWERTASVLISVLNATNL
SPKDIAALGITNQRETTLVWNRHTGRPYYNAIVWQDTRTDRIASALDRDGRGNLIRRKAGLPPATYFSGGKLQWILENVD
GVRAAAENGDALFGTPDTWVLWNLTGGPRGGVHVTDVTNASRTMLMDLETLDWDDELLSLFSIPRAMLPEIASSAPSEPY
GVTLATGPVGGEVPITGVLGDQHAAMVGQVCLAPGEAKNTYGTGNFLLLNTGETIVRSNNGLLTTVCYQFGNAKPVYALE
GSIAVTGSAVQWLRDQLGIISGAAQSEALARQVPDNGGMYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEA
ICYQSRDVVDAMEADSGVRLQVLKVDGGITGNDLCMQIQADVLGVDVVRPVVAETTALGVAYAAGLAVGFWAAPSDLRAN
WREDKRWTPTWDDDERAAGYAGWRKAVQRTLDWVDVS
>Mature_516_residues
SDAILGEQLAESSDFIAAIDQGTTSTRCMIFDHHGAEVARHQLEHEQILPRAGWVEHNPVEIWERTASVLISVLNATNLS
PKDIAALGITNQRETTLVWNRHTGRPYYNAIVWQDTRTDRIASALDRDGRGNLIRRKAGLPPATYFSGGKLQWILENVDG
VRAAAENGDALFGTPDTWVLWNLTGGPRGGVHVTDVTNASRTMLMDLETLDWDDELLSLFSIPRAMLPEIASSAPSEPYG
VTLATGPVGGEVPITGVLGDQHAAMVGQVCLAPGEAKNTYGTGNFLLLNTGETIVRSNNGLLTTVCYQFGNAKPVYALEG
SIAVTGSAVQWLRDQLGIISGAAQSEALARQVPDNGGMYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAI
CYQSRDVVDAMEADSGVRLQVLKVDGGITGNDLCMQIQADVLGVDVVRPVVAETTALGVAYAAGLAVGFWAAPSDLRANW
REDKRWTPTWDDDERAAGYAGWRKAVQRTLDWVDVS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=509, Percent_Identity=48.9194499017682, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI4504007, Length=509, Percent_Identity=48.9194499017682, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI42794763, Length=514, Percent_Identity=48.443579766537, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI41393575, Length=508, Percent_Identity=47.244094488189, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI88196792, Length=516, Percent_Identity=30.8139534883721, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1790361, Length=502, Percent_Identity=51.99203187251, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1790005, Length=479, Percent_Identity=22.964509394572, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787789, Length=551, Percent_Identity=25.5898366606171, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789987, Length=509, Percent_Identity=23.9685658153242, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=508, Percent_Identity=45.2755905511811, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71986140, Length=526, Percent_Identity=23.7642585551331, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=26.6666666666667, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321755, Length=556, Percent_Identity=39.7482014388489, Blast_Score=341, Evalue=2e-94,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=48.1481481481481, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24654573, Length=439, Percent_Identity=50.3416856492027, Blast_Score=423, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24655555, Length=523, Percent_Identity=40.9177820267686, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655560, Length=523, Percent_Identity=40.9177820267686, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655570, Length=523, Percent_Identity=40.9177820267686, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655574, Length=523, Percent_Identity=40.9177820267686, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655565, Length=523, Percent_Identity=40.9177820267686, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19922058, Length=538, Percent_Identity=35.8736059479554, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24652871, Length=504, Percent_Identity=35.515873015873, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI21357867, Length=538, Percent_Identity=29.5539033457249, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24656367, Length=533, Percent_Identity=30.0187617260788, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24656372, Length=377, Percent_Identity=31.0344827586207, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45552909, Length=318, Percent_Identity=29.2452830188679, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_MYCTA (A5U920)

Other databases:

- EMBL:   CP000611
- RefSeq:   YP_001285082.1
- ProteinModelPortal:   A5U920
- SMR:   A5U920
- STRING:   A5U920
- EnsemblBacteria:   EBMYCT00000065838
- GeneID:   5214476
- GenomeReviews:   CP000611_GR
- KEGG:   mra:MRA_3731
- eggNOG:   COG0554
- GeneTree:   EBGT00050000017511
- HOGENOM:   HBG511469
- OMA:   DSGVRLE
- ProtClustDB:   PRK00047
- BioCyc:   MTUB419947:MRA_3731-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55860; Mature: 55729

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 24-24 BINDING 28-28 BINDING 94-94 BINDING 146-146 BINDING 261-261 BINDING 283-283 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAILGEQLAESSDFIAAIDQGTTSTRCMIFDHHGAEVARHQLEHEQILPRAGWVEHNP
CCCCHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCH
VEIWERTASVLISVLNATNLSPKDIAALGITNQRETTLVWNRHTGRPYYNAIVWQDTRTD
HHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCHH
RIASALDRDGRGNLIRRKAGLPPATYFSGGKLQWILENVDGVRAAAENGDALFGTPDTWV
HHHHHHCCCCCCCEEEECCCCCCCCEECCCEEEEEEECCCCHHHHCCCCCEEECCCCCEE
LWNLTGGPRGGVHVTDVTNASRTMLMDLETLDWDDELLSLFSIPRAMLPEIASSAPSEPY
EEEECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
GVTLATGPVGGEVPITGVLGDQHAAMVGQVCLAPGEAKNTYGTGNFLLLNTGETIVRSNN
EEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEECCC
GLLTTVCYQFGNAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQSEALARQVPDNGGMY
CEEEEEHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEE
FVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVVDAMEADSGVRL
EEECCCCHHCHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCEE
QVLKVDGGITGNDLCMQIQADVLGVDVVRPVVAETTALGVAYAAGLAVGFWAAPSDLRAN
EEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCC
WREDKRWTPTWDDDERAAGYAGWRKAVQRTLDWVDVS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDAILGEQLAESSDFIAAIDQGTTSTRCMIFDHHGAEVARHQLEHEQILPRAGWVEHNP
CCCHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCH
VEIWERTASVLISVLNATNLSPKDIAALGITNQRETTLVWNRHTGRPYYNAIVWQDTRTD
HHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCHH
RIASALDRDGRGNLIRRKAGLPPATYFSGGKLQWILENVDGVRAAAENGDALFGTPDTWV
HHHHHHCCCCCCCEEEECCCCCCCCEECCCEEEEEEECCCCHHHHCCCCCEEECCCCCEE
LWNLTGGPRGGVHVTDVTNASRTMLMDLETLDWDDELLSLFSIPRAMLPEIASSAPSEPY
EEEECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
GVTLATGPVGGEVPITGVLGDQHAAMVGQVCLAPGEAKNTYGTGNFLLLNTGETIVRSNN
EEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEECCC
GLLTTVCYQFGNAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQSEALARQVPDNGGMY
CEEEEEHHHCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEE
FVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVVDAMEADSGVRL
EEECCCCHHCHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCEE
QVLKVDGGITGNDLCMQIQADVLGVDVVRPVVAETTALGVAYAAGLAVGFWAAPSDLRAN
EEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHCC
WREDKRWTPTWDDDERAAGYAGWRKAVQRTLDWVDVS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA