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The map label for this gene is cysD [H]

Identifier: 148661072

GI number: 148661072

Start: 1439219

End: 1440217

Strand: Direct

Name: cysD [H]

Synonym: MRA_1293

Alternate gene names: 148661072

Gene position: 1439219-1440217 (Clockwise)

Preceding gene: 148661071

Following gene: 148661073

Centisome position: 32.56

GC content: 64.46

Gene sequence:

>999_bases
ATGGCAATAACCATAAATATGGTCAATCCTACCGGATTTATCAGGTATGAGGACGTGGAACAGGAAGCCATGACCAGCGA
TGTGACGGTGGGCCCCGCACCCGGCCAGTACCAACTGAGCCATCTGCGCTTGCTGGAGGCCGAAGCCATCCACGTCATCC
GGGAGGTGGCCGCCGAGTTCGAGCGGCCAGTGCTGTTGTTCTCGGGGGGCAAGGACTCCATCGTCATGCTGCACCTGGCG
CTGAAGGCGTTTCGGCCCGGGCGACTGCCGTTCCCGGTCATGCACGTCGACACCGGTCACAACTTCGACGAAGTTATCGC
TACCCGAGACGAGTTGGTCGCCGCGGCCGGGGTGCGGCTGGTGGTGGCGTCGGTGCAGGACGATATCGATGCCGGTCGGG
TCGTCGAGACCATCCCGTCGCGAAATCCGATACAGACCGTGACGCTGCTGCGGGCCATCCGGGAGAACCAATTCGACGCG
GCATTCGGGGGAGCCCGGCGCGACGAGGAGAAGGCCCGCGCCAAGGAGCGGGTGTTCAGCTTCCGCGACGAGTTCGGCCA
GTGGGACCCGAAGGCTCAGCGGCCGGAACTGTGGAACCTCTACAACGGACGGCACCACAAGGGCGAGCACATCCGGGTCT
TCCCGCTGTCCAACTGGACCGAATTCGACATCTGGTCCTACATCGGCGCCGAGCAGGTCAGGCTGCCGTCCATCTATTTC
GCCCACCGGCGCAAGGTGTTTCAGCGCGACGGCATGTTGCTGGCCGTGCACCGGCACATGCAACCGCGAGCCGACGAGCC
GGTGTTCGAGGCCACGGTGCGATTCCGCACCGTCGGGGATGTTACCTGCACCGGGTGCGTCGAGTCGTCGGCATCGACGG
TCGCGGAAGTCATCGCCGAAACTGCGGTGGCCCGCTTGACGGAGCGCGGGGCGACCAGGGCTGACGACCGGATCTCGGAG
GCTGGAATGGAAGACCGCAAGCGGCAGGGATACTTCTGA

Upstream 100 bases:

>100_bases
GCCCTAGCAGCCCGAGCCGTCAGCCTAGGGCGCACTGGCGCACCGGCAGCCCGCCGAGATGGGGCTGCGTTGACAGCGAT
AGGGAAGCCTGGTTGCATAG

Downstream 100 bases:

>100_bases
TGACGACGCTATTGCGGCTGGCGACAGCGGGTTCCGTCGACGATGGCAAGTCCACGCTGATTGGGCGGCTACTCTACGAC
TCCAAGGCTGTGATGGAAGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAITINMVNPTGFIRYEDVEQEAMTSDVTVGPAPGQYQLSHLRLLEAEAIHVIREVAAEFERPVLLFSGGKDSIVMLHLA
LKAFRPGRLPFPVMHVDTGHNFDEVIATRDELVAAAGVRLVVASVQDDIDAGRVVETIPSRNPIQTVTLLRAIRENQFDA
AFGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAEQVRLPSIYF
AHRRKVFQRDGMLLAVHRHMQPRADEPVFEATVRFRTVGDVTCTGCVESSASTVAEVIAETAVARLTERGATRADDRISE
AGMEDRKRQGYF

Sequences:

>Translated_332_residues
MAITINMVNPTGFIRYEDVEQEAMTSDVTVGPAPGQYQLSHLRLLEAEAIHVIREVAAEFERPVLLFSGGKDSIVMLHLA
LKAFRPGRLPFPVMHVDTGHNFDEVIATRDELVAAAGVRLVVASVQDDIDAGRVVETIPSRNPIQTVTLLRAIRENQFDA
AFGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAEQVRLPSIYF
AHRRKVFQRDGMLLAVHRHMQPRADEPVFEATVRFRTVGDVTCTGCVESSASTVAEVIAETAVARLTERGATRADDRISE
AGMEDRKRQGYF
>Mature_331_residues
AITINMVNPTGFIRYEDVEQEAMTSDVTVGPAPGQYQLSHLRLLEAEAIHVIREVAAEFERPVLLFSGGKDSIVMLHLAL
KAFRPGRLPFPVMHVDTGHNFDEVIATRDELVAAAGVRLVVASVQDDIDAGRVVETIPSRNPIQTVTLLRAIRENQFDAA
FGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAEQVRLPSIYFA
HRRKVFQRDGMLLAVHRHMQPRADEPVFEATVRFRTVGDVTCTGCVESSASTVAEVIAETAVARLTERGATRADDRISEA
GMEDRKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=52.4752475247525, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 37513; Mature: 37382

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITINMVNPTGFIRYEDVEQEAMTSDVTVGPAPGQYQLSHLRLLEAEAIHVIREVAAEF
CEEEEEEECCCCEEEECCHHHHHHHCCCEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ERPVLLFSGGKDSIVMLHLALKAFRPGRLPFPVMHVDTGHNFDEVIATRDELVAAAGVRL
CCCEEEEECCCCCEEHHEEHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHE
VVASVQDDIDAGRVVETIPSRNPIQTVTLLRAIRENQFDAAFGGARRDEEKARAKERVFS
EEEHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHH
FRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAEQVRLPSIYF
HHHHHCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCEECCHHHH
AHRRKVFQRDGMLLAVHRHMQPRADEPVFEATVRFRTVGDVTCTGCVESSASTVAEVIAE
HHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHEEEEEECCEEEEHHHHHHHHHHHHHHHH
TAVARLTERGATRADDRISEAGMEDRKRQGYF
HHHHHHHHCCCCCCHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
AITINMVNPTGFIRYEDVEQEAMTSDVTVGPAPGQYQLSHLRLLEAEAIHVIREVAAEF
EEEEEEECCCCEEEECCHHHHHHHCCCEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ERPVLLFSGGKDSIVMLHLALKAFRPGRLPFPVMHVDTGHNFDEVIATRDELVAAAGVRL
CCCEEEEECCCCCEEHHEEHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHE
VVASVQDDIDAGRVVETIPSRNPIQTVTLLRAIRENQFDAAFGGARRDEEKARAKERVFS
EEEHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHH
FRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAEQVRLPSIYF
HHHHHCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCEECCHHHH
AHRRKVFQRDGMLLAVHRHMQPRADEPVFEATVRFRTVGDVTCTGCVESSASTVAEVIAE
HHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHEEEEEECCEEEEHHHHHHHHHHHHHHHH
TAVARLTERGATRADDRISEAGMEDRKRQGYF
HHHHHHHHCCCCCCHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]