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The map label for this gene is purD

Identifier: 148660549

GI number: 148660549

Start: 865892

End: 867160

Strand: Direct

Name: purD

Synonym: MRA_0781

Alternate gene names: 148660549

Gene position: 865892-867160 (Clockwise)

Preceding gene: 148660548

Following gene: 148660552

Centisome position: 19.59

GC content: 67.06

Gene sequence:

>1269_bases
GTGCGCGTCCTGGTAATCGGTTCGGGTGCCCGCGAACATGCGCTATTGCTGGCGCTCGGCAAAGACCCGCAGGTTTCGGG
GCTAATCGTTGCTCCCGGCAATGCAGGCACCGCTCGGATCGCCGAGCAGCACGACGTCGACATCACCTCCGCCGAGGCGG
TGGTCGCCCTGGCTCGCGAAGTCGGCGCTGACATGGTGGTGATTGGCCCCGAGGTACCGTTAGTGCTCGGGGTGGCCGAC
GCCGTGCGCGCGGCCGGCATCGTGTGTTTCGGGCCCGGTAAGGACGCGGCTCGCATCGAAGGCTCCAAAGCATTCGCCAA
GGACGTCATGGCGGCGGCCGGTGTGCGCACCGCGAACAGCGAAATCGTAGACAGCCCAGCGCACTTGGACGCGGCCCTGG
ACCGGTTCGGGCCGCCTGCCGGTGACCCGGCCTGGGTGGTCAAAGACGACCGGCTAGCCGCCGGCAAGGGTGTGGTGGTG
ACAGCGGACCGCGATGTCGCGCGCGCACACGGAGCTGCCCTGCTCGAGGCCGGGCACCCGGTGTTGCTGGAGTCCTACCT
GGACGGCCCGGAGGTATCGCTGTTCTGTGTCGTCGACCGCACCGTCGTGGTGCCGCTGCTGCCGGCACAGGACTTCAAGC
GAGTCGGTGAGGACGACACCGGACTTAACACCGGCGGTATGGGCGCCTACGCGCCGCTGCCGTGGTTGCCCGACAACATC
TATCGGGAGGTGGTCAGCCGGATCGTCGAACCCGTTGCGGCCGAACTAGTCCGGCGTGGAAGCTCGTTTTGCGGATTGCT
GTATGTTGGTCTCGCGATTACCGCCCGCGGGCCGGCGGTGGTCGAGTTCAACTGCCGATTCGGCGATCCGGAGACCCAAG
CCGTGCTGGCCTTGCTGGAGTCTCCGCTCGGCCAACTGCTTCATGCCGCCGCTACCGGGAAGCTGGCCGATTTCGGCGAG
TTGCGGTGGCGTGACGGTGTGGCCGTAACAGTGGTACTGGCGGCCGAAAACTATCCCGGGCGCCCCCGGGTCGGCGACGT
CGTTGTCGGCTCCGAAGCCGAGGGGGTGCTGCACGCCGGAACCACGCGGCGCGACGATGGCGCGATCGTTTCGTCCGGTG
GCCGGGTGCTGTCGGTGGTGGGCACCGGTGCCGACTTGTCCGCAGCACGCGCACACGCGTATGAAATCCTCAGTTCAATT
CGGTTGCCAGGAGGTCATTTCCGCAGCGATATCGGTTTACGGGCGGCCGAGGGGAAGATCAGCGTCTAG

Upstream 100 bases:

>100_bases
GTAAGCAGGCGGCCGAGAACGGCTCGCTGCCCGACACGAAAGCCAACGTCGCCGAAGTTCTTGCTAAGGAATCTGGTAAA
TCGAGCTAGTCTGACGTGTC

Downstream 100 bases:

>100_bases
CAGGCTGCGGCTTGGCCATCACGGCGGGGATCGCTGGCCGCGAGGTACCCATCGTCGAGCCGCCAGATTGCCTGACAACT
CCCGAACTGGCTGTAGTCCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MRVLVIGSGAREHALLLALGKDPQVSGLIVAPGNAGTARIAEQHDVDITSAEAVVALAREVGADMVVIGPEVPLVLGVAD
AVRAAGIVCFGPGKDAARIEGSKAFAKDVMAAAGVRTANSEIVDSPAHLDAALDRFGPPAGDPAWVVKDDRLAAGKGVVV
TADRDVARAHGAALLEAGHPVLLESYLDGPEVSLFCVVDRTVVVPLLPAQDFKRVGEDDTGLNTGGMGAYAPLPWLPDNI
YREVVSRIVEPVAAELVRRGSSFCGLLYVGLAITARGPAVVEFNCRFGDPETQAVLALLESPLGQLLHAAATGKLADFGE
LRWRDGVAVTVVLAAENYPGRPRVGDVVVGSEAEGVLHAGTTRRDDGAIVSSGGRVLSVVGTGADLSAARAHAYEILSSI
RLPGGHFRSDIGLRAAEGKISV

Sequences:

>Translated_422_residues
MRVLVIGSGAREHALLLALGKDPQVSGLIVAPGNAGTARIAEQHDVDITSAEAVVALAREVGADMVVIGPEVPLVLGVAD
AVRAAGIVCFGPGKDAARIEGSKAFAKDVMAAAGVRTANSEIVDSPAHLDAALDRFGPPAGDPAWVVKDDRLAAGKGVVV
TADRDVARAHGAALLEAGHPVLLESYLDGPEVSLFCVVDRTVVVPLLPAQDFKRVGEDDTGLNTGGMGAYAPLPWLPDNI
YREVVSRIVEPVAAELVRRGSSFCGLLYVGLAITARGPAVVEFNCRFGDPETQAVLALLESPLGQLLHAAATGKLADFGE
LRWRDGVAVTVVLAAENYPGRPRVGDVVVGSEAEGVLHAGTTRRDDGAIVSSGGRVLSVVGTGADLSAARAHAYEILSSI
RLPGGHFRSDIGLRAAEGKISV
>Mature_422_residues
MRVLVIGSGAREHALLLALGKDPQVSGLIVAPGNAGTARIAEQHDVDITSAEAVVALAREVGADMVVIGPEVPLVLGVAD
AVRAAGIVCFGPGKDAARIEGSKAFAKDVMAAAGVRTANSEIVDSPAHLDAALDRFGPPAGDPAWVVKDDRLAAGKGVVV
TADRDVARAHGAALLEAGHPVLLESYLDGPEVSLFCVVDRTVVVPLLPAQDFKRVGEDDTGLNTGGMGAYAPLPWLPDNI
YREVVSRIVEPVAAELVRRGSSFCGLLYVGLAITARGPAVVEFNCRFGDPETQAVLALLESPLGQLLHAAATGKLADFGE
LRWRDGVAVTVVLAAENYPGRPRVGDVVVGSEAEGVLHAGTTRRDDGAIVSSGGRVLSVVGTGADLSAARAHAYEILSSI
RLPGGHFRSDIGLRAAEGKISV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=38.4074941451991, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=38.4074941451991, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=38.4074941451991, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=38.4074941451991, Blast_Score=280, Evalue=1e-75,
Organism=Escherichia coli, GI1790438, Length=431, Percent_Identity=42.4593967517401, Blast_Score=304, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=37.0023419203747, Blast_Score=230, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6321203, Length=453, Percent_Identity=36.2030905077263, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62471619, Length=433, Percent_Identity=39.7228637413395, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24582400, Length=433, Percent_Identity=39.7228637413395, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_MYCBO (P65894)

Other databases:

- EMBL:   BX248336
- RefSeq:   NP_854453.1
- ProteinModelPortal:   P65894
- SMR:   P65894
- EnsemblBacteria:   EBMYCT00000017748
- GeneID:   1092501
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0795
- GeneTree:   EBGT00050000015554
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   MBOV233413:MB0795-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 43509; Mature: 43509

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVIGSGAREHALLLALGKDPQVSGLIVAPGNAGTARIAEQHDVDITSAEAVVALARE
CEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHH
VGADMVVIGPEVPLVLGVADAVRAAGIVCFGPGKDAARIEGSKAFAKDVMAAAGVRTANS
HCCCEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCCC
EIVDSPAHLDAALDRFGPPAGDPAWVVKDDRLAAGKGVVVTADRDVARAHGAALLEAGHP
HHCCCCHHHHHHHHHCCCCCCCCCEEEECCCEECCCCEEEECCCHHHHHCCCCEECCCCC
VLLESYLDGPEVSLFCVVDRTVVVPLLPAQDFKRVGEDDTGLNTGGMGAYAPLPWLPDNI
EEEHHHCCCCCEEEEEEECCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
YREVVSRIVEPVAAELVRRGSSFCGLLYVGLAITARGPAVVEFNCRFGDPETQAVLALLE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCEEEEEEECCCCCHHHHHHHHHH
SPLGQLLHAAATGKLADFGELRWRDGVAVTVVLAAENYPGRPRVGDVVVGSEAEGVLHAG
HHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCEEECCCCCCEEECC
TTRRDDGAIVSSGGRVLSVVGTGADLSAARAHAYEILSSIRLPGGHFRSDIGLRAAEGKI
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
SV
CC
>Mature Secondary Structure
MRVLVIGSGAREHALLLALGKDPQVSGLIVAPGNAGTARIAEQHDVDITSAEAVVALARE
CEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHH
VGADMVVIGPEVPLVLGVADAVRAAGIVCFGPGKDAARIEGSKAFAKDVMAAAGVRTANS
HCCCEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCCC
EIVDSPAHLDAALDRFGPPAGDPAWVVKDDRLAAGKGVVVTADRDVARAHGAALLEAGHP
HHCCCCHHHHHHHHHCCCCCCCCCEEEECCCEECCCCEEEECCCHHHHHCCCCEECCCCC
VLLESYLDGPEVSLFCVVDRTVVVPLLPAQDFKRVGEDDTGLNTGGMGAYAPLPWLPDNI
EEEHHHCCCCCEEEEEEECCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
YREVVSRIVEPVAAELVRRGSSFCGLLYVGLAITARGPAVVEFNCRFGDPETQAVLALLE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEECCCCEEEEEEECCCCCHHHHHHHHHH
SPLGQLLHAAATGKLADFGELRWRDGVAVTVVLAAENYPGRPRVGDVVVGSEAEGVLHAG
HHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCEEECCCCCCEEECC
TTRRDDGAIVSSGGRVLSVVGTGADLSAARAHAYEILSSIRLPGGHFRSDIGLRAAEGKI
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
SV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972