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The map label for this gene is sppA [H]

Identifier: 148660499

GI number: 148660499

Start: 816969

End: 818840

Strand: Direct

Name: sppA [H]

Synonym: MRA_0732

Alternate gene names: 148660499

Gene position: 816969-818840 (Clockwise)

Preceding gene: 148660498

Following gene: 148660504

Centisome position: 18.48

GC content: 66.99

Gene sequence:

>1872_bases
ATGCCCATTTTCGGGGGCTTTTGCGTCTGCTCGCGGGCCCTTGGCGGCCGGTGGGTACGCTGGGTGAATATGGTTGCCTT
TCTGCCTTCCATTCCCGTTGTCGAGGACCTACGCGCCCTGGTCGGCCGGGTTGATACCGCCCGCCACCACGGTGTACCCA
ACGGCTGCGTGCTCGAATTCAACCTGCGATCGGTGCCGCCGGAGACGACGGGCTTCGACCCTCTTACGGTGCTCACCGGG
GGTGGGCGGCCGATGGCGCTGCGCGATGCGGTCGCCGCGATCCACCGTGCCGCCGAGGACCCCCGGGTAGCCGGGCTGAT
AGCCCGCGTGCAGCTTCCGCCCTCGCCGGCGGGGGCGGTTCAGGAGCTGCGGGAGGCCATCGCGGCCTTCAGTGCGGTCA
AGCCGTCGCTGGCCTGGGCCGAAACTTATCCGGGCACCCTGTCCTACTATCTGGCTTCGGCGTTCGGTGAGGTCTGGATG
CAACCCTCGGGGAGTGTGGGGCTGGTCGGCTTCGCCACCAACGCCACATTCCTGCGCGACGCCCTGCACAAGGCGGGCAT
CGAGGCCCAGTTCGTCGCCCGGGGCGAATACAAGTCGGCGGCAAACCTTTTCACCGAGGATGGCTTCACAGACGCCCACC
GCGAAGCGGTCACGCGGATGCTGGACAGTCTGCAGGACCAGGTGTGGCAGGCGGTCGCCAAGTCGCGCAATATCGGCGTC
GATGCGCTTGATGAGCTGGCTGACCGGGCTCCGCTATTGCGGGACGACGCCGTGACTTGCGGTCTGATCGACCGGATCGG
ATTTCGCGACCAAGCCTACGCCCGTATGGCGGAATTGGTTGGTGTGGAAAAAGGTTCACCGGAATCCAGTGGCTCGCAAA
CAAGCCCAGACGAAAAGCCGCCGCGGATGTACCTGGCGCGCTACGCCAGTTCGGCCCGGCCACGGCTGACGCCCCCCGTC
CCATCGATTCCTGGTCGCCGGTCCAAGCCGACGATCGCGGTGGTGACCCTGGAAGGCCCGATCGTCAACGGTCGTGGTGG
GCCCCAGTTTCTGCCGCTCGGTCCGTCGAGCGCCGGCGGTGACACCATCGCGGCAGCGCTGCGGGAGGTGGCCGCCGACG
ATTCGGTGTCGGCGATAGTGCTGCGGGTCGACAGTCCGGGGGGCTCGGTCACCGCATCGGAGACTATCTGGCGTGAGGTG
GCCAGGGCCCGCGACCGTGGCAAACCGGTGGTGGCGTCGATGGGTGCGGTCGCCGCCTCCGGTGGCTATTACGTGTCGAT
GGGTGCCGACGCCATCGTGGCCAACCCGGGCACCATCACCGGGTCGATCGGTGTGATCACCGGAAAGCTGGTGGTTCGGG
ATCTCAAGGACCGGTTGGGTGTCGGGTCGGATGCGGTGCGCACCAACGCTAATGCCGATGCCTGGTCGATCGACGCACCC
TTCACCCCGGACCAGCAGGCCCATCGCGAGGCGGAGGCGGACTTGTTCTACAGCGACTTCGTGGAACGCGTCGCCGAGGG
CCGCAAGATGACTACCGACGCCGTGGACGTCGTTGCGCGAGGCCGGGTCTGGACCGGTGCCGACGCTCTCGATCGCGGCC
TGGTCGACGAACTCGGCGGCCTTCGAACCGCGGTGCGTCGCGCGAAGGTGCTAGCCGGACTAGATGAGGACACCGAGGTT
CGCATAGTCAGTTATCCGGGGTCGTCACTCTGGGACATGGTGCGACCGCGTCCGTCGTCACGACCGGCAGCGGCATCGCT
GCCGGATGCTATGGGTGCGCTGCTTGCCCGTTCGATCGTCGGCATCGTCGAGCAGGTGGAACAGACTCTCAGTGGTGCCA
GCGTGTTGTGGCTGGGGGAGTCGCGCCTCTAG

Upstream 100 bases:

>100_bases
CGCACAAGTTCAGCGGCAGCGCGCGCGCGAAGATCACCGCAGCGGGCGGTTCAGCCACCGAGCTCTAGTTTCGGGCGAGC
AGACGCAAAATGCCCCCGAA

Downstream 100 bases:

>100_bases
CCGTTCAAACGACCGCTGATGAAGATGATTTCGCCGAGCGGATCGTCGTCGTGTGGGGCGGGAACGGGCAAACCATTGCG
CCTGAATAGGTCGGTCCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV homolog; Signal peptide peptidase [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MPIFGGFCVCSRALGGRWVRWVNMVAFLPSIPVVEDLRALVGRVDTARHHGVPNGCVLEFNLRSVPPETTGFDPLTVLTG
GGRPMALRDAVAAIHRAAEDPRVAGLIARVQLPPSPAGAVQELREAIAAFSAVKPSLAWAETYPGTLSYYLASAFGEVWM
QPSGSVGLVGFATNATFLRDALHKAGIEAQFVARGEYKSAANLFTEDGFTDAHREAVTRMLDSLQDQVWQAVAKSRNIGV
DALDELADRAPLLRDDAVTCGLIDRIGFRDQAYARMAELVGVEKGSPESSGSQTSPDEKPPRMYLARYASSARPRLTPPV
PSIPGRRSKPTIAVVTLEGPIVNGRGGPQFLPLGPSSAGGDTIAAALREVAADDSVSAIVLRVDSPGGSVTASETIWREV
ARARDRGKPVVASMGAVAASGGYYVSMGADAIVANPGTITGSIGVITGKLVVRDLKDRLGVGSDAVRTNANADAWSIDAP
FTPDQQAHREAEADLFYSDFVERVAEGRKMTTDAVDVVARGRVWTGADALDRGLVDELGGLRTAVRRAKVLAGLDEDTEV
RIVSYPGSSLWDMVRPRPSSRPAAASLPDAMGALLARSIVGIVEQVEQTLSGASVLWLGESRL

Sequences:

>Translated_623_residues
MPIFGGFCVCSRALGGRWVRWVNMVAFLPSIPVVEDLRALVGRVDTARHHGVPNGCVLEFNLRSVPPETTGFDPLTVLTG
GGRPMALRDAVAAIHRAAEDPRVAGLIARVQLPPSPAGAVQELREAIAAFSAVKPSLAWAETYPGTLSYYLASAFGEVWM
QPSGSVGLVGFATNATFLRDALHKAGIEAQFVARGEYKSAANLFTEDGFTDAHREAVTRMLDSLQDQVWQAVAKSRNIGV
DALDELADRAPLLRDDAVTCGLIDRIGFRDQAYARMAELVGVEKGSPESSGSQTSPDEKPPRMYLARYASSARPRLTPPV
PSIPGRRSKPTIAVVTLEGPIVNGRGGPQFLPLGPSSAGGDTIAAALREVAADDSVSAIVLRVDSPGGSVTASETIWREV
ARARDRGKPVVASMGAVAASGGYYVSMGADAIVANPGTITGSIGVITGKLVVRDLKDRLGVGSDAVRTNANADAWSIDAP
FTPDQQAHREAEADLFYSDFVERVAEGRKMTTDAVDVVARGRVWTGADALDRGLVDELGGLRTAVRRAKVLAGLDEDTEV
RIVSYPGSSLWDMVRPRPSSRPAAASLPDAMGALLARSIVGIVEQVEQTLSGASVLWLGESRL
>Mature_622_residues
PIFGGFCVCSRALGGRWVRWVNMVAFLPSIPVVEDLRALVGRVDTARHHGVPNGCVLEFNLRSVPPETTGFDPLTVLTGG
GRPMALRDAVAAIHRAAEDPRVAGLIARVQLPPSPAGAVQELREAIAAFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQ
PSGSVGLVGFATNATFLRDALHKAGIEAQFVARGEYKSAANLFTEDGFTDAHREAVTRMLDSLQDQVWQAVAKSRNIGVD
ALDELADRAPLLRDDAVTCGLIDRIGFRDQAYARMAELVGVEKGSPESSGSQTSPDEKPPRMYLARYASSARPRLTPPVP
SIPGRRSKPTIAVVTLEGPIVNGRGGPQFLPLGPSSAGGDTIAAALREVAADDSVSAIVLRVDSPGGSVTASETIWREVA
RARDRGKPVVASMGAVAASGGYYVSMGADAIVANPGTITGSIGVITGKLVVRDLKDRLGVGSDAVRTNANADAWSIDAPF
TPDQQAHREAEADLFYSDFVERVAEGRKMTTDAVDVVARGRVWTGADALDRGLVDELGGLRTAVRRAKVLAGLDEDTEVR
IVSYPGSSLWDMVRPRPSSRPAAASLPDAMGALLARSIVGIVEQVEQTLSGASVLWLGESRL

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=525, Percent_Identity=34.0952380952381, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1787527, Length=170, Percent_Identity=32.3529411764706, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 65936; Mature: 65805

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFGGFCVCSRALGGRWVRWVNMVAFLPSIPVVEDLRALVGRVDTARHHGVPNGCVLEF
CCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
NLRSVPPETTGFDPLTVLTGGGRPMALRDAVAAIHRAAEDPRVAGLIARVQLPPSPAGAV
ECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCHHH
QELREAIAAFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGSVGLVGFATNATFLRD
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHH
ALHKAGIEAQFVARGEYKSAANLFTEDGFTDAHREAVTRMLDSLQDQVWQAVAKSRNIGV
HHHHCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DALDELADRAPLLRDDAVTCGLIDRIGFRDQAYARMAELVGVEKGSPESSGSQTSPDEKP
HHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PRMYLARYASSARPRLTPPVPSIPGRRSKPTIAVVTLEGPIVNGRGGPQFLPLGPSSAGG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCCEEEECCCCCCCH
DTIAAALREVAADDSVSAIVLRVDSPGGSVTASETIWREVARARDRGKPVVASMGAVAAS
HHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCEEECCCHHHCC
GGYYVSMGADAIVANPGTITGSIGVITGKLVVRDLKDRLGVGSDAVRTNANADAWSIDAP
CCEEEEECCCEEEECCCCEECCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEECCCC
FTPDQQAHREAEADLFYSDFVERVAEGRKMTTDAVDVVARGRVWTGADALDRGLVDELGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHH
LRTAVRRAKVLAGLDEDTEVRIVSYPGSSLWDMVRPRPSSRPAAASLPDAMGALLARSIV
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
GIVEQVEQTLSGASVLWLGESRL
HHHHHHHHHHCCCEEEEECCCCC
>Mature Secondary Structure 
PIFGGFCVCSRALGGRWVRWVNMVAFLPSIPVVEDLRALVGRVDTARHHGVPNGCVLEF
CCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
NLRSVPPETTGFDPLTVLTGGGRPMALRDAVAAIHRAAEDPRVAGLIARVQLPPSPAGAV
ECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCHHH
QELREAIAAFSAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGSVGLVGFATNATFLRD
HHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHH
ALHKAGIEAQFVARGEYKSAANLFTEDGFTDAHREAVTRMLDSLQDQVWQAVAKSRNIGV
HHHHCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DALDELADRAPLLRDDAVTCGLIDRIGFRDQAYARMAELVGVEKGSPESSGSQTSPDEKP
HHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PRMYLARYASSARPRLTPPVPSIPGRRSKPTIAVVTLEGPIVNGRGGPQFLPLGPSSAGG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCCEEEECCCCCCCH
DTIAAALREVAADDSVSAIVLRVDSPGGSVTASETIWREVARARDRGKPVVASMGAVAAS
HHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCEEECCCHHHCC
GGYYVSMGADAIVANPGTITGSIGVITGKLVVRDLKDRLGVGSDAVRTNANADAWSIDAP
CCEEEEECCCEEEECCCCEECCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEECCCC
FTPDQQAHREAEADLFYSDFVERVAEGRKMTTDAVDVVARGRVWTGADALDRGLVDELGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHH
LRTAVRRAKVLAGLDEDTEVRIVSYPGSSLWDMVRPRPSSRPAAASLPDAMGALLARSIV
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
GIVEQVEQTLSGASVLWLGESRL
HHHHHHHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]