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The map label for this gene is thcD [H]

Identifier: 148660463

GI number: 148660463

Start: 789246

End: 790466

Strand: Direct

Name: thcD [H]

Synonym: MRA_0697

Alternate gene names: 148660463

Gene position: 789246-790466 (Clockwise)

Preceding gene: 148660462

Following gene: 148660467

Centisome position: 17.86

GC content: 64.86

Gene sequence:

>1221_bases
ATGAACGCACACGTGACCAGTCGTGAAGGCGTCAATGAGTTTGACGATGGAATTGTGATCGTCGGCGGCGGATTGGCAGC
TGCGCGCACCGCCGAGCAGTTGCGTCGTGCGGGCTATTCGGGTCGCCTCACGATCGTCAGCGACGAGGTGCATCTGCCGT
ACGACCGTCCGCCGCTATCCAAGGAGGTGCTGCGCAGCGAGGTCGACGATGTGGCCCTCAAACCCCGCGAGTTCTACGAC
GAAAAGGACATCGCACTTCGGCTGGGGTCGGCTGCCGTCAGCTTGGACACGGGAGAACAGACGGTAACGCTGGCCGACGG
TACGGTGCTCGGCTACGACGAGCTCGTCATCGCGACTGGTTTGGTGCCCCGGCGTATTCCATCGCTTCCCGACCTTGATG
GCATTCGGGTGCTCCGGTCGTTCGACGAGAGCATGGCACTGCGCAAGCATGCATCCGCCGCACGGCACGCCGTGGTGGTG
GGGGCCGGTTTCATCGGCTGCGAGGTGGCTGCCAGTCTGCGCGGTCTCGGTGTGGATGTGGTGCTGGTTGAGCCGCAGCC
GGCGCCGTTGGCCTCGGTGCTGGGCGAGCAGATCGGCCAGTTGGTGACGCGGCTCCATCGCGATGAGGGCGTTGATGTTC
GCACGGGTGTGACAGTGGCCGAGGTACGTGGCAAGGGGCATGTCGACGCGGTGGTCCTGACCGACGGTACCGAACTGCCG
GCTGATCTGGTGGTTGTGGGCATTGGGTCGACCCCGGCGACCGAATGGCTAGAGGGTAGCGGCGTCGAGGTCGACAACGG
CGTGATCTGTGACAAAGCCGGGCGGACTAGCGCGCCGAATGTGTGGGCGCTCGGTGACGTCGCCTCCTGGCGAGATCCGA
TGGGACACCAAGCACGCGTGGAACATTGGAGCAACGTCGCCGACCAGGCCCGAGTCGTGGTGCCCGCGATGCTCGGGACC
GATGTGCCCACGGGCGTGGTCGTCCCGTATTTCTGGAGTGACCAGTATGACGTCAAAATCCAGTGCCTGGGGGAGCCGCA
CGCCACCGACGTTGTGCATCTGGTCGAGGACGACGGGCGCAAGTTCCTTGCCTATTACGAGCGCGATGGCGTGCTGGTTG
GCGTGGTCGGTGGCGGGATGGCCGGCAAGGTCATGAAGGTGCGCGGCAAGATCGCCGCGGGCGCGCCCATCGCCGAAGTG
TTAGACCAAACTCAGGCCTAG

Upstream 100 bases:

>100_bases
GACGTCGTTGTCTGGTTGGCCGGCGACGGCTCGGGCGCACTGTCGGGCAATCAGATCCCGGTCGATAAGGGTGCCTTGAA
GTATTGACGCGCGATCGTGT

Downstream 100 bases:

>100_bases
AGCTGACCTAGGTGGCAGCGGGCGCCCTGGTCGTCGGCGCATTCGGCGGACATATCGTCTGGCTGTCGGGACGGCTCGGC
CAGCGCGCCGGCCGCACGCA

Product: ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNAHVTSREGVNEFDDGIVIVGGGLAAARTAEQLRRAGYSGRLTIVSDEVHLPYDRPPLSKEVLRSEVDDVALKPREFYD
EKDIALRLGSAAVSLDTGEQTVTLADGTVLGYDELVIATGLVPRRIPSLPDLDGIRVLRSFDESMALRKHASAARHAVVV
GAGFIGCEVAASLRGLGVDVVLVEPQPAPLASVLGEQIGQLVTRLHRDEGVDVRTGVTVAEVRGKGHVDAVVLTDGTELP
ADLVVVGIGSTPATEWLEGSGVEVDNGVICDKAGRTSAPNVWALGDVASWRDPMGHQARVEHWSNVADQARVVVPAMLGT
DVPTGVVVPYFWSDQYDVKIQCLGEPHATDVVHLVEDDGRKFLAYYERDGVLVGVVGGGMAGKVMKVRGKIAAGAPIAEV
LDQTQA

Sequences:

>Translated_406_residues
MNAHVTSREGVNEFDDGIVIVGGGLAAARTAEQLRRAGYSGRLTIVSDEVHLPYDRPPLSKEVLRSEVDDVALKPREFYD
EKDIALRLGSAAVSLDTGEQTVTLADGTVLGYDELVIATGLVPRRIPSLPDLDGIRVLRSFDESMALRKHASAARHAVVV
GAGFIGCEVAASLRGLGVDVVLVEPQPAPLASVLGEQIGQLVTRLHRDEGVDVRTGVTVAEVRGKGHVDAVVLTDGTELP
ADLVVVGIGSTPATEWLEGSGVEVDNGVICDKAGRTSAPNVWALGDVASWRDPMGHQARVEHWSNVADQARVVVPAMLGT
DVPTGVVVPYFWSDQYDVKIQCLGEPHATDVVHLVEDDGRKFLAYYERDGVLVGVVGGGMAGKVMKVRGKIAAGAPIAEV
LDQTQA
>Mature_406_residues
MNAHVTSREGVNEFDDGIVIVGGGLAAARTAEQLRRAGYSGRLTIVSDEVHLPYDRPPLSKEVLRSEVDDVALKPREFYD
EKDIALRLGSAAVSLDTGEQTVTLADGTVLGYDELVIATGLVPRRIPSLPDLDGIRVLRSFDESMALRKHASAARHAVVV
GAGFIGCEVAASLRGLGVDVVLVEPQPAPLASVLGEQIGQLVTRLHRDEGVDVRTGVTVAEVRGKGHVDAVVLTDGTELP
ADLVVVGIGSTPATEWLEGSGVEVDNGVICDKAGRTSAPNVWALGDVASWRDPMGHQARVEHWSNVADQARVVVPAMLGT
DVPTGVVVPYFWSDQYDVKIQCLGEPHATDVVHLVEDDGRKFLAYYERDGVLVGVVGGGMAGKVMKVRGKIAAGAPIAEV
LDQTQA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=390, Percent_Identity=31.7948717948718, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI226437568, Length=390, Percent_Identity=31.7948717948718, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI65787454, Length=390, Percent_Identity=31.7948717948718, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI22202629, Length=335, Percent_Identity=29.2537313432836, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4757732, Length=335, Percent_Identity=29.2537313432836, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI195927006, Length=163, Percent_Identity=29.4478527607362, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788892, Length=383, Percent_Identity=32.1148825065274, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=27.4647887323944, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=24.9211356466877, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082354, Length=302, Percent_Identity=25.4966887417219, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786307, Length=191, Percent_Identity=31.9371727748691, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=376, Percent_Identity=31.3829787234043, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32564386, Length=332, Percent_Identity=29.5180722891566, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24639257, Length=357, Percent_Identity=32.4929971988796, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI281359715, Length=357, Percent_Identity=32.4929971988796, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI281359713, Length=357, Percent_Identity=32.4929971988796, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24639250, Length=357, Percent_Identity=32.4929971988796, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI18543267, Length=357, Percent_Identity=32.4929971988796, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24639252, Length=357, Percent_Identity=32.4929971988796, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24585130, Length=360, Percent_Identity=30.5555555555556, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24581020, Length=361, Percent_Identity=30.4709141274238, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28573993, Length=361, Percent_Identity=30.4709141274238, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43018; Mature: 43018

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHVTSREGVNEFDDGIVIVGGGLAAARTAEQLRRAGYSGRLTIVSDEVHLPYDRPPLS
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCC
KEVLRSEVDDVALKPREFYDEKDIALRLGSAAVSLDTGEQTVTLADGTVLGYDELVIATG
HHHHHHHHHHHHCCCHHHCCCCCEEEEECCEEEEECCCCEEEEEECCEEECCCHHEEECC
LVPRRIPSLPDLDGIRVLRSFDESMALRKHASAARHAVVVGAGFIGCEVAASLRGLGVDV
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCCEEE
VLVEPQPAPLASVLGEQIGQLVTRLHRDEGVDVRTGVTVAEVRGKGHVDAVVLTDGTELP
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCEEEEEEECCCCCC
ADLVVVGIGSTPATEWLEGSGVEVDNGVICDKAGRTSAPNVWALGDVASWRDPMGHQARV
CEEEEEECCCCCHHHHHCCCCCEECCCEEECCCCCCCCCCEEEECCCHHHCCCCCCHHHH
EHWSNVADQARVVVPAMLGTDVPTGVVVPYFWSDQYDVKIQCLGEPHATDVVHLVEDDGR
HHHHHHHHHHEEEEEHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCC
KFLAYYERDGVLVGVVGGGMAGKVMKVRGKIAAGAPIAEVLDQTQA
EEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNAHVTSREGVNEFDDGIVIVGGGLAAARTAEQLRRAGYSGRLTIVSDEVHLPYDRPPLS
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCC
KEVLRSEVDDVALKPREFYDEKDIALRLGSAAVSLDTGEQTVTLADGTVLGYDELVIATG
HHHHHHHHHHHHCCCHHHCCCCCEEEEECCEEEEECCCCEEEEEECCEEECCCHHEEECC
LVPRRIPSLPDLDGIRVLRSFDESMALRKHASAARHAVVVGAGFIGCEVAASLRGLGVDV
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHCCCEEE
VLVEPQPAPLASVLGEQIGQLVTRLHRDEGVDVRTGVTVAEVRGKGHVDAVVLTDGTELP
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCEEEEEEECCCCCC
ADLVVVGIGSTPATEWLEGSGVEVDNGVICDKAGRTSAPNVWALGDVASWRDPMGHQARV
CEEEEEECCCCCHHHHHCCCCCEECCCEEECCCCCCCCCCEEEECCCHHHCCCCCCHHHH
EHWSNVADQARVVVPAMLGTDVPTGVVVPYFWSDQYDVKIQCLGEPHATDVVHLVEDDGR
HHHHHHHHHHEEEEEHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCC
KFLAYYERDGVLVGVVGGGMAGKVMKVRGKIAAGAPIAEVLDQTQA
EEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]