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The map label for this gene is tuf [H]

Identifier: 148660460

GI number: 148660460

Start: 786127

End: 787317

Strand: Direct

Name: tuf [H]

Synonym: MRA_0694

Alternate gene names: 148660460

Gene position: 786127-787317 (Clockwise)

Preceding gene: 148660459

Following gene: 148660461

Centisome position: 17.79

GC content: 64.15

Gene sequence:

>1191_bases
GTGGCGAAGGCGAAGTTCCAGCGGACCAAGCCCCACGTCAACATCGGGACCATCGGTCACGTTGACCACGGCAAGACCAC
CCTGACCGCGGCTATCACCAAGGTCCTGCACGACAAATTCCCCGATCTGAACGAGACGAAGGCATTCGACCAGATCGACA
ACGCCCCCGAGGAGCGTCAGCGCGGTATCACCATCAACATCGCGCACGTGGAGTACCAGACCGACAAGCGGCACTACGCA
CACGTCGACGCCCCTGGCCACGCCGACTACATCAAGAACATGATCACCGGCGCCGCGCAGATGGACGGTGCGATCCTGGT
GGTCGCCGCCACCGACGGCCCGATGCCCCAGACCCGCGAGCACGTTCTGCTGGCGCGTCAAGTGGGTGTGCCCTACATCC
TGGTAGCGCTGAACAAGGCCGACGCAGTGGACGACGAGGAGCTGCTCGAACTCGTCGAGATGGAGGTCCGCGAGCTGCTG
GCTGCCCAGGAATTCGACGAGGACGCCCCGGTTGTGCGGGTCTCGGCGCTCAAGGCGCTCGAGGGTGACGCGAAGTGGGT
TGCCTCTGTCGAGGAACTGATGAACGCGGTCGACGAGTCGATTCCGGACCCGGTCCGCGAGACCGACAAGCCGTTCCTGA
TGCCGGTCGAGGACGTCTTCACCATTACCGGCCGCGGAACCGTGGTCACCGGACGTGTGGAGCGCGGCGTGATCAACGTG
AACGAGGAAGTTGAGATCGTCGGCATTCGCCCATCGACCACCAAGACCACCGTCACCGGTGTGGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCGGGCGACAACGTTGGTTTGCTGCTGCGGGGCGTCAAGCGCGAGGACGTCGAGCGTGGCCAGG
TTGTCACCAAGCCCGGCACCACCACGCCGCACACCGAGTTCGAAGGCCAGGTCTACATCCTGTCCAAGGACGAGGGCGGC
CGGCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGTGTGGTGACACTGCC
GGAGGGCACCGAGATGGTGATGCCCGGTGACAACACCAACATCTCGGTGAAGTTGATCCAGCCCGTCGCCATGGACGAAG
GTCTGCGTTTCGCGATCCGCGAGGGTGGCCGCACCGTGGGCGCCGGCCGGGTCACCAAGATCATCAAGTAG

Upstream 100 bases:

>100_bases
ATACAACCATACGAGTAGGCTGGCCCGGTTACGACCGCGGCATAACTGAAAACATCAACACTGCTTTTATAAGCACTAAC
AAGTCCAGGAGGACACAAAA

Downstream 100 bases:

>100_bases
GTCTACCGGCCACCAGACGCAAAAGAACATGATGGGCGCACCAGCGCCCATCATGTTCTTTTGCGTCTGCTCGCGAAAAT
GCCCAGCGTGCGGCGCTACG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQRGITINIAHVEYQTDKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELL
AAQEFDEDAPVVRVSALKALEGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
NEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK

Sequences:

>Translated_396_residues
MAKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQRGITINIAHVEYQTDKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELL
AAQEFDEDAPVVRVSALKALEGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
NEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK
>Mature_395_residues
AKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQRGITINIAHVEYQTDKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELLA
AQEFDEDAPVVRVSALKALEGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINVN
EEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=401, Percent_Identity=55.6109725685786, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI4503471, Length=455, Percent_Identity=31.2087912087912, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=33.6158192090396, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI223555963, Length=438, Percent_Identity=30.8219178082192, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI5729864, Length=438, Percent_Identity=30.8219178082192, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=28.486646884273, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=28.486646884273, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=28.486646884273, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=27.6923076923077, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI46094014, Length=338, Percent_Identity=28.4023668639053, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4503507, Length=307, Percent_Identity=26.7100977198697, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI82546879, Length=427, Percent_Identity=21.5456674473068, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=236, Percent_Identity=26.6949152542373, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=74.4949494949495, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=74.4949494949495, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=31.2684365781711, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789108, Length=326, Percent_Identity=29.1411042944785, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48994988, Length=283, Percent_Identity=31.8021201413428, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=25.1515151515152, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=48.2587064676617, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=45.1871657754011, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=31.0112359550562, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=31.0112359550562, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=29.5348837209302, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=27.5462962962963, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=27.5462962962963, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=30.7692307692308, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=24, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=214, Percent_Identity=32.7102803738318, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=26.6891891891892, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=25.8620689655172, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565438, Length=423, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=404, Percent_Identity=62.1287128712871, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325337, Length=348, Percent_Identity=35.3448275862069, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=348, Percent_Identity=35.3448275862069, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=356, Percent_Identity=27.247191011236, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=348, Percent_Identity=29.3103448275862, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=281, Percent_Identity=27.0462633451957, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=28.8702928870293, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=138, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=58.8383838383838, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=58.8383838383838, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.3856041131105, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=31.3063063063063, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=31.3063063063063, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=31.3063063063063, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=31.3063063063063, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24652838, Length=445, Percent_Identity=30.3370786516854, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI17137572, Length=445, Percent_Identity=30.3370786516854, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.1475409836066, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=31.1764705882353, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=30.1775147928994, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=26.4285714285714, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=26.4285714285714, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=188, Percent_Identity=36.1702127659575, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI78706572, Length=263, Percent_Identity=28.1368821292776, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43594; Mature: 43463

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQ
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
RGITINIAHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
EGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHEEEEECCCEEEEEEEECCEEEC
NEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGT
CCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEECCCC
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK
EEEEEECCCCCCCCCEEEECCCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQ
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
RGITINIAHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
EGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEHHHEEEEECCCEEEEEEEECCEEEC
NEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGT
CCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEECCCC
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK
EEEEEECCCCCCCCCEEEECCCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA