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Identifier: 148660445

GI number: 148660445

Start: 771098

End: 771856

Strand: Direct

Name: end

Synonym: MRA_0680

Alternate gene names: 148660445

Gene position: 771098-771856 (Clockwise)

Preceding gene: 148660442

Following gene: 148660446

Centisome position: 17.45

GC content: 66.14

Gene sequence:

>759_bases
GTGCTCATTGGTTCGCATGTCAGCCCAACCGATCCGCTGGCCGCAGCGGAGGCCGAAGGCGCTGACGTAGTGCAGATTTT
CCTTGGCAATCCGCAGAGCTGGAAGGCTCCCAAGCCGCGGGACGACGCCGCCGCGCTGAAAGCCGCGACCCTGCCCATCT
ACGTGCATGCGCCCTACCTGATCAACCTTGCGTCGGCGAACAATCGCGTGCGGATCCCGTCGCGCAAGATCCTGCAAGAG
ACCTGTGCTGCGGCGGCCGACATTGGCGCAGCGGCGGTGATCGTGCACGGTGGGCACGTCGCCGACGACAACGACATCGA
CAAGGGCTTCCAGCGCTGGCGCAAGGCGCTGGACCGGCTGGAAACCGAGGTTCCCGTCTACCTGGAAAACACCGCCGGCG
GCGATCACGCGATGGCGCGCCGCTTCGACACCATCGCCCGGCTCTGGGACGTCATCGGCGACACCGGAATCGGGTTTTGC
CTGGACACCTGCCACACCTGGGCGGCCGGCGAGGCGCTGACCGATGCCGTCGATCGGATCAAAGCAATTACCGGCCGCAT
CGATCTGGTGCACTGCAACGACTCCAGGGACGAAGCGGGATCGGGCCGTGACCGCCACGCCAACCTCGGCAGCGGCCAGA
TTGATCCTGACCTGCTGGTGGCTGCCGTCAAGGCGGCCGGCGCGCCGGTGATCTGCGAAACCGCCGACCAAGGTCGCAAG
GACGACATCGCGTTTCTGCGGGAAAGAACCGGCAGCTGA

Upstream 100 bases:

>100_bases
CGGGGCCCACGAATACGGACGGCAGCATCCCGTGCCGCGGCTGGCCGGACGTGATCCGAGGGTGTGGGTCTCACCAGATC
GGTCTCACTAGACTTGGGTT

Downstream 100 bases:

>100_bases
CTTCAAGCCCCGCGGCACCTACCGTTGACTTATGCTCCGCAGGGTCGCCATACTGCTCGCCGCTGTGCTTGCGTTCGCGG
GCTGCTCGGGGGGAACGAGG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLIGSHVSPTDPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYLINLASANNRVRIPSRKILQE
TCAAAADIGAAAVIVHGGHVADDNDIDKGFQRWRKALDRLETEVPVYLENTAGGDHAMARRFDTIARLWDVIGDTGIGFC
LDTCHTWAAGEALTDAVDRIKAITGRIDLVHCNDSRDEAGSGRDRHANLGSGQIDPDLLVAAVKAAGAPVICETADQGRK
DDIAFLRERTGS

Sequences:

>Translated_252_residues
MLIGSHVSPTDPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYLINLASANNRVRIPSRKILQE
TCAAAADIGAAAVIVHGGHVADDNDIDKGFQRWRKALDRLETEVPVYLENTAGGDHAMARRFDTIARLWDVIGDTGIGFC
LDTCHTWAAGEALTDAVDRIKAITGRIDLVHCNDSRDEAGSGRDRHANLGSGQIDPDLLVAAVKAAGAPVICETADQGRK
DDIAFLRERTGS
>Mature_252_residues
MLIGSHVSPTDPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYLINLASANNRVRIPSRKILQE
TCAAAADIGAAAVIVHGGHVADDNDIDKGFQRWRKALDRLETEVPVYLENTAGGDHAMARRFDTIARLWDVIGDTGIGFC
LDTCHTWAAGEALTDAVDRIKAITGRIDLVHCNDSRDEAGSGRDRHANLGSGQIDPDLLVAAVKAAGAPVICETADQGRK
DDIAFLRERTGS

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=260, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17531193, Length=273, Percent_Identity=24.5421245421245, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322735, Length=277, Percent_Identity=24.5487364620939, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_MYCBO (P63536)

Other databases:

- EMBL:   BX248336
- RefSeq:   NP_854347.1
- ProteinModelPortal:   P63536
- SMR:   P63536
- EnsemblBacteria:   EBMYCT00000015572
- GeneID:   1091807
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0689
- GeneTree:   EBGT00050000017851
- HOGENOM:   HBG565018
- OMA:   NRIRIPS
- ProtClustDB:   PRK01060
- BioCyc:   MBOV233413:MB0689-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 26845; Mature: 26845

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGSHVSPTDPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYL
CCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHEEEEEEECCEE
INLASANNRVRIPSRKILQETCAAAADIGAAAVIVHGGHVADDNDIDKGFQRWRKALDRL
EEEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ETEVPVYLENTAGGDHAMARRFDTIARLWDVIGDTGIGFCLDTCHTWAAGEALTDAVDRI
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHH
KAITGRIDLVHCNDSRDEAGSGRDRHANLGSGQIDPDLLVAAVKAAGAPVICETADQGRK
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCH
DDIAFLRERTGS
HHHHHHHHHCCC
>Mature Secondary Structure
MLIGSHVSPTDPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYL
CCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHEEEEEEECCEE
INLASANNRVRIPSRKILQETCAAAADIGAAAVIVHGGHVADDNDIDKGFQRWRKALDRL
EEEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ETEVPVYLENTAGGDHAMARRFDTIARLWDVIGDTGIGFCLDTCHTWAAGEALTDAVDRI
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHH
KAITGRIDLVHCNDSRDEAGSGRDRHANLGSGQIDPDLLVAAVKAAGAPVICETADQGRK
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCH
DDIAFLRERTGS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972