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The map label for this gene is 148660444

Identifier: 148660444

GI number: 148660444

Start: 768990

End: 770903

Strand: Reverse

Name: 148660444

Synonym: MRA_0679

Alternate gene names: NA

Gene position: 770903-768990 (Counterclockwise)

Preceding gene: 148660451

Following gene: 148660443

Centisome position: 17.44

GC content: 65.2

Gene sequence:

>1914_bases
ATGCTTAGTGTAGGGCGCGGCATCGCCGACATCACTGGTGAGGCGGCGGACTGCGGCATGCTCGGCTACGGCAAGTCCGA
CCAGCGCACCGCCGGCATTCACCAGCGACTTAGGTCACGGGCGTTTGTGTTCCGGGACGATTCCCAGGACGGGGACGCGC
GGTTGCTGCTGATTGTCGCCGAACTGCCGCTGCCCATGCAAAACGTGAATGAGGAAGTGCTGCGCCGGTTGGCCGATTTG
TATGGCGATACCTACTCGGAGCAGAACACACTGATCACGGCGACCCACACCCACGCCGGGCCTGGCGGGTACTGCGGATA
CCTGCTGTACAACCTGACCACGAGCGGCTTTCGGCCGGCAACCTTTGCGGCGATCGTCGACGGAATTGTCGAATCGGTGG
AACATGCGCATGCCGATGTGGCGCCCGCCGAGGTTTCGCTCTCGCACGGCGAGCTGTACGGCGCAAGCATCAATCGATCC
CCATCAGCGTTCGACCGCAACCCGCCGGCCGACAAGGCGTTCTTCCCGAAACGCGTCGACCCGCATACCACGCTCGTGCG
TATCGACCGGGGCGAGGCCACCGTGGGCGTGATCCATTTCTTCGCTACCCACGGTACGAGCATGACGAATCGCAACCACC
TCATCTCCGGAGACAACAAGGGTTTCGCGGCCTATCACTGGGAACGCACCGTGGGCGGCGCGGATTATCTCGCCGGCCAG
CCCGACTTCATCGCCGCCTTCGCACAGACGAATCCGGGCGACATGAGCCCGAATGTCGACGGGCCCCTTTCTCCCGAGGC
CCCACCAGACCGGGAATTCGACAACACCCGTCGTACCGGGTTGTGCCAGTTCGAGGATGCTTTCACGCAGTTGAGCGGAG
CGACGCCCATCGGCGCTGGCATTGATGCCCGGTTCACCTATGTCGATCTCGGTTCTGTGCTCGTGCGCGGCGAGTACACA
CCAGACGGTGAAGAGCGCCGCACCGGGCGTCCGATGTTTGGCGCCGGGGCCATGGCAGGCACCGACGAGGGCCCGGGCTT
CCATGGCTTTCGCCAAGGGCGAAACCCGTTCTGGGACAGACTATCCCGCGCCATGTACCGGCTGGCAAGGCCGACGGCAG
CGGCACAGGCCCCCAAAGGCATTGTGATGCCCGCACGCCTGCCGAACCGGATACACCCCTTTGTCCAGGAAATTGTTCCC
GTTCAGCTCGTGCGAATCGGTCGCCTCTATCTGATCGGTATTCCGGGCGAACCGACCATCGTCGCGGGGCTTAGGCTGCG
CCGCATGGTGGCTTCGATTGTTGGCGCCGACCTGGCCGACGTGCTCTGCGTGGGCTACACCAACGCCTACATCCATTACG
TGACAACGCCCGAAGAGTATCTCGAACAGCGCTACGAGGGCGGCAGCACGTTGTTCGGTCGATGGGAACTGTGCGCCCTC
ATGCAGACCGTGGCCGAACTCGCCGAGGCCATGCGCGACGGCCGTCCGGTGACGCTCGGTCGCCGGCCGCGGCCCACCCG
GGAGCTGAGCTGGGTGCGCGGCGCGCCCGCCGATGCCGGGTCGTTCGGAGCGGTCATCGCCGAGCCCTCGGCGACGTACC
GACCGGGTCAGGCGGTGGAAGCCGTTTTCGTCAGCGCGCTGCCCAATAACGATCTGCGCCGCGGCGGCACCTACCTCGAA
GTGGTCCGCCGGGAAGGTGCCAGCTGGGTGCGGATTGCTGACGACGGCGACTGGGCCACGAGCTTTCGCTGGCAGCGCCA
AGGGCGGGCCGGATCGCATGTCAGCATTCGCTGGGATGTTCCCGGCGATACCACGCCCGGGCAATACCGCATCGTCCACC
ACGGCACCGCACGCGATCGCAACGGCATGCTTACAGCGTTCAGCGCGACGACGCGCGAGTTCACGGTTGTTTGA

Upstream 100 bases:

>100_bases
GCCCCGGCGCATAAAGTGTTTCTCCTTGTGGGATACGTTTCCCCGGAGCAGCGCGTAGTCAGCGCTCGTGAAATTTGCTG
CTGGTGCGGAGGATTAGGCA

Downstream 100 bases:

>100_bases
CTCGGCGGCCGCAATCACCAACCCAGGCCATGCCTGGGCGTCGGCGATGGAACGCAGTGGCCTGCTGGAATGCGTGGCCG
GCTTGGACGAGCAACCGTTC

Product: putative hydrolase

Products: NA

Alternate protein names: N-CDase; NCDase; Acylsphingosine deacylase; N-acylsphingosine amidohydrolase [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MLSVGRGIADITGEAADCGMLGYGKSDQRTAGIHQRLRSRAFVFRDDSQDGDARLLLIVAELPLPMQNVNEEVLRRLADL
YGDTYSEQNTLITATHTHAGPGGYCGYLLYNLTTSGFRPATFAAIVDGIVESVEHAHADVAPAEVSLSHGELYGASINRS
PSAFDRNPPADKAFFPKRVDPHTTLVRIDRGEATVGVIHFFATHGTSMTNRNHLISGDNKGFAAYHWERTVGGADYLAGQ
PDFIAAFAQTNPGDMSPNVDGPLSPEAPPDREFDNTRRTGLCQFEDAFTQLSGATPIGAGIDARFTYVDLGSVLVRGEYT
PDGEERRTGRPMFGAGAMAGTDEGPGFHGFRQGRNPFWDRLSRAMYRLARPTAAAQAPKGIVMPARLPNRIHPFVQEIVP
VQLVRIGRLYLIGIPGEPTIVAGLRLRRMVASIVGADLADVLCVGYTNAYIHYVTTPEEYLEQRYEGGSTLFGRWELCAL
MQTVAELAEAMRDGRPVTLGRRPRPTRELSWVRGAPADAGSFGAVIAEPSATYRPGQAVEAVFVSALPNNDLRRGGTYLE
VVRREGASWVRIADDGDWATSFRWQRQGRAGSHVSIRWDVPGDTTPGQYRIVHHGTARDRNGMLTAFSATTREFTVV

Sequences:

>Translated_637_residues
MLSVGRGIADITGEAADCGMLGYGKSDQRTAGIHQRLRSRAFVFRDDSQDGDARLLLIVAELPLPMQNVNEEVLRRLADL
YGDTYSEQNTLITATHTHAGPGGYCGYLLYNLTTSGFRPATFAAIVDGIVESVEHAHADVAPAEVSLSHGELYGASINRS
PSAFDRNPPADKAFFPKRVDPHTTLVRIDRGEATVGVIHFFATHGTSMTNRNHLISGDNKGFAAYHWERTVGGADYLAGQ
PDFIAAFAQTNPGDMSPNVDGPLSPEAPPDREFDNTRRTGLCQFEDAFTQLSGATPIGAGIDARFTYVDLGSVLVRGEYT
PDGEERRTGRPMFGAGAMAGTDEGPGFHGFRQGRNPFWDRLSRAMYRLARPTAAAQAPKGIVMPARLPNRIHPFVQEIVP
VQLVRIGRLYLIGIPGEPTIVAGLRLRRMVASIVGADLADVLCVGYTNAYIHYVTTPEEYLEQRYEGGSTLFGRWELCAL
MQTVAELAEAMRDGRPVTLGRRPRPTRELSWVRGAPADAGSFGAVIAEPSATYRPGQAVEAVFVSALPNNDLRRGGTYLE
VVRREGASWVRIADDGDWATSFRWQRQGRAGSHVSIRWDVPGDTTPGQYRIVHHGTARDRNGMLTAFSATTREFTVV
>Mature_637_residues
MLSVGRGIADITGEAADCGMLGYGKSDQRTAGIHQRLRSRAFVFRDDSQDGDARLLLIVAELPLPMQNVNEEVLRRLADL
YGDTYSEQNTLITATHTHAGPGGYCGYLLYNLTTSGFRPATFAAIVDGIVESVEHAHADVAPAEVSLSHGELYGASINRS
PSAFDRNPPADKAFFPKRVDPHTTLVRIDRGEATVGVIHFFATHGTSMTNRNHLISGDNKGFAAYHWERTVGGADYLAGQ
PDFIAAFAQTNPGDMSPNVDGPLSPEAPPDREFDNTRRTGLCQFEDAFTQLSGATPIGAGIDARFTYVDLGSVLVRGEYT
PDGEERRTGRPMFGAGAMAGTDEGPGFHGFRQGRNPFWDRLSRAMYRLARPTAAAQAPKGIVMPARLPNRIHPFVQEIVP
VQLVRIGRLYLIGIPGEPTIVAGLRLRRMVASIVGADLADVLCVGYTNAYIHYVTTPEEYLEQRYEGGSTLFGRWELCAL
MQTVAELAEAMRDGRPVTLGRRPRPTRELSWVRGAPADAGSFGAVIAEPSATYRPGQAVEAVFVSALPNNDLRRGGTYLE
VVRREGASWVRIADDGDWATSFRWQRQGRAGSHVSIRWDVPGDTTPGQYRIVHHGTARDRNGMLTAFSATTREFTVV

Specific function: Hydrolyzes the ceramides into sphingosine and free fatty acid at an optimal pH of 8-9 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral ceramidase family [H]

Homologues:

Organism=Homo sapiens, GI221218981, Length=684, Percent_Identity=32.6023391812866, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI221307475, Length=660, Percent_Identity=33.6363636363636, Blast_Score=343, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24651405, Length=684, Percent_Identity=30.8479532163743, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24651403, Length=684, Percent_Identity=30.8479532163743, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24651401, Length=684, Percent_Identity=30.8479532163743, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24651397, Length=684, Percent_Identity=30.8479532163743, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24651399, Length=684, Percent_Identity=30.8479532163743, Blast_Score=291, Evalue=7e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006823 [H]

Pfam domain/function: NA

EC number: =3.5.1.23 [H]

Molecular weight: Translated: 69491; Mature: 69491

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVGRGIADITGEAADCGMLGYGKSDQRTAGIHQRLRSRAFVFRDDSQDGDARLLLIVA
CCCCCCCHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEE
ELPLPMQNVNEEVLRRLADLYGDTYSEQNTLITATHTHAGPGGYCGYLLYNLTTSGFRPA
CCCCCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHEECCCCCCCHH
TFAAIVDGIVESVEHAHADVAPAEVSLSHGELYGASINRSPSAFDRNPPADKAFFPKRVD
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PHTTLVRIDRGEATVGVIHFFATHGTSMTNRNHLISGDNKGFAAYHWERTVGGADYLAGQ
CCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCEECCCCCCEEEEEEEECCCCCHHCCCC
PDFIAAFAQTNPGDMSPNVDGPLSPEAPPDREFDNTRRTGLCQFEDAFTQLSGATPIGAG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
IDARFTYVDLGSVLVRGEYTPDGEERRTGRPMFGAGAMAGTDEGPGFHGFRQGRNPFWDR
CCCEEEEEECCCEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHH
LSRAMYRLARPTAAAQAPKGIVMPARLPNRIHPFVQEIVPVQLVRIGRLYLIGIPGEPTI
HHHHHHHHHCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHEECCEEEEECCCCCCE
VAGLRLRRMVASIVGADLADVLCVGYTNAYIHYVTTPEEYLEQRYEGGSTLFGRWELCAL
EHHHHHHHHHHHHHCCCHHHHHHEEECCEEEEEEECHHHHHHHHHCCCCEEEHHHHHHHH
MQTVAELAEAMRDGRPVTLGRRPRPTRELSWVRGAPADAGSFGAVIAEPSATYRPGQAVE
HHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHH
AVFVSALPNNDLRRGGTYLEVVRREGASWVRIADDGDWATSFRWQRQGRAGSHVSIRWDV
HHHEECCCCCHHHCCCHHHHHHHHCCCCEEEEECCCCCHHCEEHHHCCCCCCEEEEEEEC
PGDTTPGQYRIVHHGTARDRNGMLTAFSATTREFTVV
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEC
>Mature Secondary Structure
MLSVGRGIADITGEAADCGMLGYGKSDQRTAGIHQRLRSRAFVFRDDSQDGDARLLLIVA
CCCCCCCHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEE
ELPLPMQNVNEEVLRRLADLYGDTYSEQNTLITATHTHAGPGGYCGYLLYNLTTSGFRPA
CCCCCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHEECCCCCCCHH
TFAAIVDGIVESVEHAHADVAPAEVSLSHGELYGASINRSPSAFDRNPPADKAFFPKRVD
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PHTTLVRIDRGEATVGVIHFFATHGTSMTNRNHLISGDNKGFAAYHWERTVGGADYLAGQ
CCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCEECCCCCCEEEEEEEECCCCCHHCCCC
PDFIAAFAQTNPGDMSPNVDGPLSPEAPPDREFDNTRRTGLCQFEDAFTQLSGATPIGAG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
IDARFTYVDLGSVLVRGEYTPDGEERRTGRPMFGAGAMAGTDEGPGFHGFRQGRNPFWDR
CCCEEEEEECCCEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHH
LSRAMYRLARPTAAAQAPKGIVMPARLPNRIHPFVQEIVPVQLVRIGRLYLIGIPGEPTI
HHHHHHHHHCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHEECCEEEEECCCCCCE
VAGLRLRRMVASIVGADLADVLCVGYTNAYIHYVTTPEEYLEQRYEGGSTLFGRWELCAL
EHHHHHHHHHHHHHCCCHHHHHHEEECCEEEEEEECHHHHHHHHHCCCCEEEHHHHHHHH
MQTVAELAEAMRDGRPVTLGRRPRPTRELSWVRGAPADAGSFGAVIAEPSATYRPGQAVE
HHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHHH
AVFVSALPNNDLRRGGTYLEVVRREGASWVRIADDGDWATSFRWQRQGRAGSHVSIRWDV
HHHEECCCCCHHHCCCHHHHHHHHCCCCEEEEECCCCCHHCEEHHHCCCCCCEEEEEEEC
PGDTTPGQYRIVHHGTARDRNGMLTAFSATTREFTVV
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10593963; 10984043 [H]