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The map label for this gene is menD

Identifier: 148660324

GI number: 148660324

Start: 647608

End: 649272

Strand: Direct

Name: menD

Synonym: MRA_0562

Alternate gene names: 148660324

Gene position: 647608-649272 (Clockwise)

Preceding gene: 148660323

Following gene: 148660325

Centisome position: 14.65

GC content: 69.73

Gene sequence:

>1665_bases
GTGAACCCCTCGACGACACAGGCGCGCGTCGTCGTCGACGAACTGATCCGCGGCGGCGTTCGCGACGTGGTGCTGTGTCC
GGGCTCGCGCAATGCGCCGCTGGCCTTCGCGCTGCAGGACGCCGACCGGTCCGGCCGGATCCGGTTGCACGTTCGCATCG
ATGAACGCACCGCCGGCTACCTGGCCATCGGGCTGGCAATCGGGGCGGGCGCGCCGGTGTGTGTCGCGATGACATCCGGC
ACCGCCGTGGCCAACCTCGGTCCGGCGGTGGTGGAGGCAAACTACGCTCGGGTGCCGCTGATCGTGCTGTCAGCCAATCG
GCCCTACGAGCTGCTGGGCACCGGCGCCAACCAGACCATGGAACAGCTGGGCTATTTCGGCACCCAGGTGCGCGCCAGCA
TCAGCCTGGGGCTGGCCGAGGACGCACCCGAGCGGACCTCGGCGCTCAACGCGACCTGGCGATCGGCTACGTGCCGAGTG
TTGGCGGCCGCCACGGGTGCTCGCACCGCCAACGCGGGCCCCGTGCACTTCGACATCCCGCTGCGCGAACCGCTGGTGCC
CGATCCCGAGCCCCTCGGCGCGGTCACCCCGCCGGGCCGGCCTGCTGGCAAGCCGTGGACCTACACGCCGCCGGTCACCT
TCGACCAGCCACTGGACATCGACCTGTCGGTCGACACCGTGGTCATCTCCGGGCATGGCGCTGGCGTGCACCCCAACCTC
GCGGCGTTGCCGACCGTCGCAGAACCGACGGCGCCGCGGTCCGGGGACAACCCGTTGCACCCGCTGGCGCTGCCGCTGCT
GCGCCCTCAACAGGTGATCATGCTGGGCCGGCCGACACTGCATCGTCCGGTATCGGTGCTGCTGGCCGACGCAGAAGTGC
CGGTATTCGCATTGACAACCGGTCCACGCTGGCCGGATGTCTCGGGTAACTCGCAGGCCACCGGCACGCGGGCGGTCACC
ACCGGCGCGCCGCGGCCCGCGTGGCTGGACCGGTGTGCGGCGATGAACCGGCACGCGATCGCGGCGGTTCGGGAACAGCT
CGCGGCGCACCCGTTGACCACCGGGCTGCATGTCGCGGCGGCGGTGTCGCATGCGCTGCGGCCCGGTGACCAGCTGGTGC
TCGGGGCATCCAATCCGGTGCGGGATGTGGCGTTGGCCGGTTTGGACACCCGCGGCATCCGGGTACGGTCCAACCGTGGG
GTCGCCGGCATCGACGGCACCGTGTCCACCGCGATCGGGGCGGCCCTAGCTTATGAGGGGGCTCACGAGCGCACCGGCAG
CCCGGACTCCCCGCCCCGCACCATCGCACTGATCGGCGACCTGACGTTCGTGCACGACAGCTCCGGGCTGTTGATCGGGC
CGACCGAACCGATACCGCGGTCATTGACCATCGTGGTGTCTAATGACAACGGCGGCGGCATCTTCGAATTGCTCGAGCAG
GGTGATCCCAGGTTCTCCGACGTGTCATCGCGAATCTTCGGCACCCCACACGACGTCGATGTGGGCGCATTGTGCCGCGC
CTACCACGTGGAATCTCGCCAGATCGAGGTCGACGAACTCGGACCGACCCTCGATCAACCCGGTGCCGGCATGCGCGTGC
TCGAGGTCAAGGCCGACCGGTCGTCGTTGCGACAATTGCACGCCGCCATCAAGGCGGCTCTGTGA

Upstream 100 bases:

>100_bases
GCGGCCGGAAGCCGTCAACACCGCGATGCTGAAGTTCTTCGCCAGTGTCAAGGCCTGAGCGCGGCCCGGCCATACGGTCC
GGCTGTGACACTCTGTACTG

Downstream 100 bases:

>100_bases
TATCACCGAAACCCCTGCTGCACATCCTGATTCATGGGCTCAGTGATGAACTGCCCGATACTCGAGGCAGGATCGTGCTG
CGCTGGTTACGAATCGCCGT

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGYLAIGLAIGAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRASISLGLAEDAPERTSALNATWRSATCRV
LAAATGARTANAGPVHFDIPLREPLVPDPEPLGAVTPPGRPAGKPWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNL
AALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTTGPRWPDVSGNSQATGTRAVT
TGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAAAVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRG
VAGIDGTVSTAIGAALAYEGAHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGIFELLEQ
GDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADRSSLRQLHAAIKAAL

Sequences:

>Translated_554_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGYLAIGLAIGAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRASISLGLAEDAPERTSALNATWRSATCRV
LAAATGARTANAGPVHFDIPLREPLVPDPEPLGAVTPPGRPAGKPWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNL
AALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTTGPRWPDVSGNSQATGTRAVT
TGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAAAVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRG
VAGIDGTVSTAIGAALAYEGAHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGIFELLEQ
GDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADRSSLRQLHAAIKAAL
>Mature_554_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGYLAIGLAIGAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRASISLGLAEDAPERTSALNATWRSATCRV
LAAATGARTANAGPVHFDIPLREPLVPDPEPLGAVTPPGRPAGKPWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNL
AALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTTGPRWPDVSGNSQATGTRAVT
TGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAAAVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRG
VAGIDGTVSTAIGAALAYEGAHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGIFELLEQ
GDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADRSSLRQLHAAIKAAL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=525, Percent_Identity=30.6666666666667, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_MYCBO (Q7U1S4)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854230.1
- ProteinModelPortal:   Q7U1S4
- EnsemblBacteria:   EBMYCT00000016979
- GeneID:   1091651
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0570
- GeneTree:   EBGT00050000015760
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- ProtClustDB:   PRK07449
- BioCyc:   MBOV233413:MB0570-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 57836; Mature: 57836

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAIGAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTM
EEEEEECCCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCEEEEECCHHHHH
EQLGYFGTQVRASISLGLAEDAPERTSALNATWRSATCRVLAAATGARTANAGPVHFDIP
HHHHCCCCEEEEEEEECCCCCCCHHHHHHCCHHCCCEEEEEEEECCCCCCCCCCEEEECC
LREPLVPDPEPLGAVTPPGRPAGKPWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNL
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCH
AALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTT
HCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEC
GPRWPDVSGNSQATGTRAVTTGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAA
CCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
AVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRGVAGIDGTVSTAIGAALAYEG
HHHHHCCCCCEEEEECCCCHHHHHEECCCCCCEEEECCCCEECCCCHHHHHHHHHHHCCC
AHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGIFELLEQ
CCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHC
GDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADR
CCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEHHHCCCCCCCCCCCEEEEEEECCH
SSLRQLHAAIKAAL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRSGRIRLHVRIDERTAGY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAIGAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTM
EEEEEECCCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCEEEEECCHHHHH
EQLGYFGTQVRASISLGLAEDAPERTSALNATWRSATCRVLAAATGARTANAGPVHFDIP
HHHHCCCCEEEEEEEECCCCCCCHHHHHHCCHHCCCEEEEEEEECCCCCCCCCCEEEECC
LREPLVPDPEPLGAVTPPGRPAGKPWTYTPPVTFDQPLDIDLSVDTVVISGHGAGVHPNL
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCH
AALPTVAEPTAPRSGDNPLHPLALPLLRPQQVIMLGRPTLHRPVSVLLADAEVPVFALTT
HCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEC
GPRWPDVSGNSQATGTRAVTTGAPRPAWLDRCAAMNRHAIAAVREQLAAHPLTTGLHVAA
CCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
AVSHALRPGDQLVLGASNPVRDVALAGLDTRGIRVRSNRGVAGIDGTVSTAIGAALAYEG
HHHHHCCCCCEEEEECCCCHHHHHEECCCCCCEEEECCCCEECCCCHHHHHHHHHHHCCC
AHERTGSPDSPPRTIALIGDLTFVHDSSGLLIGPTEPIPRSLTIVVSNDNGGGIFELLEQ
CCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHC
GDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEVDELGPTLDQPGAGMRVLEVKADR
CCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEHHHCCCCCCCCCCCEEEEEEECCH
SSLRQLHAAIKAAL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972