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The map label for this gene is pitA [H]

Identifier: 148660314

GI number: 148660314

Start: 637520

End: 638773

Strand: Reverse

Name: pitA [H]

Synonym: MRA_0552

Alternate gene names: 148660314

Gene position: 638773-637520 (Counterclockwise)

Preceding gene: 148660315

Following gene: 148660313

Centisome position: 14.45

GC content: 67.22

Gene sequence:

>1254_bases
GTGAACCTTCAGTTGTTCCTTTTGCTCATTGTCGTCGTGACGGCATTGGCGTTCGACTTCACCAACGGGTTCCACGACAC
CGGAAACGCCATGGCGACCTCGATTGCCAGCGGCGCCCTGGCACCGCGGGTAGCGGTAGCACTTCCTGCCGTGCTGAACC
TGATCGGTGCGTTTTTGTCCACCGCCGTGGCGGCCACAATCGCCAAGGGTCTGATCGACGCGAATCTGGTGACGCTGGAG
TTGGTGTTCGCCGGCCTGGTCGGCGGGATCGTCTGGAACCTGTTGACCTGGTTGCTGGGCATTCCGTCGAGTTCCTCACA
TGCGCTGATCGGCGGCATCGTCGGCGCCACAATTGCCGCCGTCGGCCTGCGTGGGGTGATCTGGAGCGGAGTGGTGTCCA
AGGTGATCGTGCCGGCCGTGGTAGCCGCGCTGCTGGCCACGCTGGTCGGAGCAGTCGGCACCTGGCTGGTCTACCGGACG
ACGCGCGGGGTTGCCGAAAAGCGTACGGAACGCGGTTTCCGGCGCGGCCAGATCGGCTCGGCGTCGCTGGTCTCGCTGGC
GCACGGCACCAACGACGCGCAGAAGACGATGGGCGTGATCTTCCTGGCGTTGATGTCCTACGGCGCGGTCAGCACGACGG
CATCGGTGCCGCCGCTGTGGGTGATCGTGAGTTGCGCCGTGGCCATGGCCGCCGGTACCTACCTGGGTGGCTGGCGCATC
ATCCGCACCCTAGGCAAAGGGCTGGTCGAGATCAAACCACCGCAGGGTATGGCCGCCGAGTCGTCATCGGCCGCCGTCAT
TCTGTTGTCCGCGCACTTCGGCTATGCGCTGTCCACAACGCAGGTCGCGACCGGGTCCGTGCTGGGCAGCGGCGTCGGCA
AGCCCGGCGCCGAGGTGCGCTGGGGGGTAGCCGGCCGCATGGTGGTCGCGTGGCTGGTGACGCTCCCGTTGGCCGGGCTG
GTCGGGGCCTTCACCTACGGGCTGGTGCATTTCATCGGTGGCTACCCCGGTGCGATCCTCGGTTTCGCACTGTTGTGGCT
GACCGCCACCGCCATCTGGCTGCGGTCGCGCAGGGCGCCGATCGACCACACCAACGTCAACGCCGACTGGGAAGGCAACC
TGACGGCCGGCCTGGAAGCGGGTGCGCAGCCGCTTGCGGATCAGAGGCCGCCGGTGCCTGCACCGCCGGCTCCGACTCCC
CCACCGAACCACCGAGCACCACAGTTCGGCGTCACCACGAGGAACGCCCCGTGA

Upstream 100 bases:

>100_bases
TACGTGATTGGTAACATCTGACGACGAGTCGAGATACACCCGCATTTCGCGTACTCGTTTGATCGACAACGCAATATCCC
GACCAGCAGAATCAGGCCCT

Downstream 100 bases:

>100_bases
GTGCCTGGTTCAACTACACGGCCACCCTGAAGATCCTGATCTTCAGTCTGCTAGCCGGCGCGCTGCTACCGGGCCTGTTC
GCGGTGGGCGTGCGGCTGCA

Product: low-affinity inorganic phosphate transporter integral membrane protein PitA

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALPAVLNLIGAFLSTAVAATIAKGLIDANLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAAVGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRT
TRGVAEKRTERGFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVRWGVAGRMVVAWLVTLPLAGL
VGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAPIDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTP
PPNHRAPQFGVTTRNAP

Sequences:

>Translated_417_residues
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALPAVLNLIGAFLSTAVAATIAKGLIDANLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAAVGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRT
TRGVAEKRTERGFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVRWGVAGRMVVAWLVTLPLAGL
VGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAPIDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTP
PPNHRAPQFGVTTRNAP
>Mature_417_residues
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALPAVLNLIGAFLSTAVAATIAKGLIDANLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAAVGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRT
TRGVAEKRTERGFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVRWGVAGRMVVAWLVTLPLAGL
VGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAPIDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTP
PPNHRAPQFGVTTRNAP

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=161, Percent_Identity=29.1925465838509, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI5803173, Length=167, Percent_Identity=31.7365269461078, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789907, Length=222, Percent_Identity=32.4324324324324, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789360, Length=223, Percent_Identity=29.5964125560538, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539280, Length=174, Percent_Identity=28.735632183908, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71981576, Length=171, Percent_Identity=30.9941520467836, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=178, Percent_Identity=30.3370786516854, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25146401, Length=172, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=166, Percent_Identity=28.3132530120482, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 42746; Mature: 42746

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQLFLLLIVVVTALAFDFTNGFHDTGNAMATSIASGALAPRVAVALPAVLNLIGAFLS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TAVAATIAKGLIDANLVTLELVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGATIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VGLRGVIWSGVVSKVIVPAVVAALLATLVGAVGTWLVYRTTRGVAEKRTERGFRRGQIGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCC
ASLVSLAHGTNDAQKTMGVIFLALMSYGAVSTTASVPPLWVIVSCAVAMAAGTYLGGWRI
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRTLGKGLVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVATGSVLGSGVGKPGAEVR
HHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEE
WGVAGRMVVAWLVTLPLAGLVGAFTYGLVHFIGGYPGAILGFALLWLTATAIWLRSRRAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
IDHTNVNADWEGNLTAGLEAGAQPLADQRPPVPAPPAPTPPPNHRAPQFGVTTRNAP
CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]