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The map label for this gene is hemA

Identifier: 148660278

GI number: 148660278

Start: 601751

End: 603157

Strand: Direct

Name: hemA

Synonym: MRA_0516

Alternate gene names: 148660278

Gene position: 601751-603157 (Clockwise)

Preceding gene: 148660277

Following gene: 148660279

Centisome position: 13.61

GC content: 66.74

Gene sequence:

>1407_bases
GTGAGCGTGCTGCTCTTCGGGGTGTCGCATCGTAGCGCGCCGGTCGTCGTCCTTGAACAACTCAGTATCGACGAATCCGA
TCAAGTCAAGATCATCGACCGAGTGCTGGCTTCGCCGCTGGTGACCGAGGCGATGGTGCTGTCGACTTGCAACCGCGTCG
AGGTCTACGCCGTAGTGGACGCGTTCCATGGCGGCCTGTCGGTGATCGGGCAGGTGCTTGCCGAACACTCCGGTATGTCG
ATGGGGGAGCTGACCAAGTACGCATATGTCCGCTACAGCGAGGCAGCAGTTGAGCACCTGTTCGCGGTTGCCAGCGGCCT
GGACTCGGCGGTGATCGGCGAGCAGCAGGTGCTTGGTCAGGTGCGCCGCGCCTATGCCGTCGCCGAATCCAACCGCACGG
TCGGCCGCGTGCTGCACGAATTGGCCCAGCGGGCGCTGTCGGTGGGCAAGCGAGTGCACTCCGAAACCGCCATTGACGCT
GCCGGTGCCTCCGTGGTGTCGGTCGCCCTGGGAATGGCCGAGCGCAAATTGGGCTCGTTGGCGGGCACGACCGCGGTGGT
GATCGGCGCCGGGGCGATGGGCGCGCTGTCGGCGGTACATCTGACCCGTGCCGGCGTCGGGCACATTCAGGTGCTCAACC
GGTCGTTGTCCCGGGCGCAGCGGTTGGCCCGAAGGATCCGCGAATCTGGCGTGCCGGCCGAGGCGCTAGCGCTCGACCGC
CTGGCTAATGTCCTGGCCGATGCCGACGTGGTGGTCAGCTGTACTGGGGCGGTGCGTCCGGTGGTGTCGCTGGCCGATGT
GCATCATGCGCTGGCCGCCGCCCGCCGTGACGAGGCCACCCGTCCGTTGGTGATATGCGACTTGGGCATGCCGCGTGACG
TCGATCCTGCGGTGGCCAGATTACCGTGTGTGTGGGTCGTGGACGTGGATAGCGTGCAACATGAACCCTCGGCACATGCC
GCGGCTGCCGACGTTGAGGCCGCCCGCCACATCGTCGCCGCCGAAGTTGCCAGCTATCTGGTGGGGCAGCGGATGGCCGA
GGTCACCCCAACCGTGACGGCGTTGCGCCAGCGAGCCGCCGAAGTGGTCGAAGCGGAATTGCTGCGCCTGGACAACCGGC
TGCCCGGCCTGCAGAGTGTCCAGCGCGAGGAGGTGGCCCGCACCGTACGGCGAGTCGTGGACAAGCTGTTGCACGCGCCT
ACCGTGCGGATCAAGCAGCTCGCCAGTGCGCCCGGCGGTGACAGCTACGCCGAGGCGCTGCGCGAACTCTTCGAGCTTGA
CCAGACCGCCGTCGATGCCGTCGCCACTGCAGGTGAATTACCGGTGGTGCCAAGCGGATTCGACGCTGAAAGTCGCCGCG
GTGGAGGCGACATGCAAAGCAGCCCGAAGCGATCGCCGAGTAACTGA

Upstream 100 bases:

>100_bases
GATATAGCCCGCAGCACATTTGGTAGCCCACCTGATAAACGTCTACCGTAGACACCAGTTTTACTGGGGTAGTCGAGGGA
GCTGGCCAGGTGGTGCTGCC

Downstream 100 bases:

>100_bases
TTGGCGCACGTGATCCGGATAGGTACCCGGGGCAGCTTGCTGGCCACCACTCAGGCCGCCACTGTCAGAGACGCCCTCAT
CGCTGGTGGCCACTCCGCGG

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSVLLFGVSHRSAPVVVLEQLSIDESDQVKIIDRVLASPLVTEAMVLSTCNRVEVYAVVDAFHGGLSVIGQVLAEHSGMS
MGELTKYAYVRYSEAAVEHLFAVASGLDSAVIGEQQVLGQVRRAYAVAESNRTVGRVLHELAQRALSVGKRVHSETAIDA
AGASVVSVALGMAERKLGSLAGTTAVVIGAGAMGALSAVHLTRAGVGHIQVLNRSLSRAQRLARRIRESGVPAEALALDR
LANVLADADVVVSCTGAVRPVVSLADVHHALAAARRDEATRPLVICDLGMPRDVDPAVARLPCVWVVDVDSVQHEPSAHA
AAADVEAARHIVAAEVASYLVGQRMAEVTPTVTALRQRAAEVVEAELLRLDNRLPGLQSVQREEVARTVRRVVDKLLHAP
TVRIKQLASAPGGDSYAEALRELFELDQTAVDAVATAGELPVVPSGFDAESRRGGGDMQSSPKRSPSN

Sequences:

>Translated_468_residues
MSVLLFGVSHRSAPVVVLEQLSIDESDQVKIIDRVLASPLVTEAMVLSTCNRVEVYAVVDAFHGGLSVIGQVLAEHSGMS
MGELTKYAYVRYSEAAVEHLFAVASGLDSAVIGEQQVLGQVRRAYAVAESNRTVGRVLHELAQRALSVGKRVHSETAIDA
AGASVVSVALGMAERKLGSLAGTTAVVIGAGAMGALSAVHLTRAGVGHIQVLNRSLSRAQRLARRIRESGVPAEALALDR
LANVLADADVVVSCTGAVRPVVSLADVHHALAAARRDEATRPLVICDLGMPRDVDPAVARLPCVWVVDVDSVQHEPSAHA
AAADVEAARHIVAAEVASYLVGQRMAEVTPTVTALRQRAAEVVEAELLRLDNRLPGLQSVQREEVARTVRRVVDKLLHAP
TVRIKQLASAPGGDSYAEALRELFELDQTAVDAVATAGELPVVPSGFDAESRRGGGDMQSSPKRSPSN
>Mature_467_residues
SVLLFGVSHRSAPVVVLEQLSIDESDQVKIIDRVLASPLVTEAMVLSTCNRVEVYAVVDAFHGGLSVIGQVLAEHSGMSM
GELTKYAYVRYSEAAVEHLFAVASGLDSAVIGEQQVLGQVRRAYAVAESNRTVGRVLHELAQRALSVGKRVHSETAIDAA
GASVVSVALGMAERKLGSLAGTTAVVIGAGAMGALSAVHLTRAGVGHIQVLNRSLSRAQRLARRIRESGVPAEALALDRL
ANVLADADVVVSCTGAVRPVVSLADVHHALAAARRDEATRPLVICDLGMPRDVDPAVARLPCVWVVDVDSVQHEPSAHAA
AADVEAARHIVAAEVASYLVGQRMAEVTPTVTALRQRAAEVVEAELLRLDNRLPGLQSVQREEVARTVRRVVDKLLHAPT
VRIKQLASAPGGDSYAEALRELFELDQTAVDAVATAGELPVVPSGFDAESRRGGGDMQSSPKRSPSN

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=424, Percent_Identity=33.0188679245283, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_MYCBO (P64329)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854184.1
- ProteinModelPortal:   P64329
- SMR:   P64329
- EnsemblBacteria:   EBMYCT00000016878
- GeneID:   1091581
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0521
- GeneTree:   EBGT00050000017163
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   MBOV233413:MB0521-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 49362; Mature: 49231

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLLFGVSHRSAPVVVLEQLSIDESDQVKIIDRVLASPLVTEAMVLSTCNRVEVYAVVD
CCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
AFHGGLSVIGQVLAEHSGMSMGELTKYAYVRYSEAAVEHLFAVASGLDSAVIGEQQVLGQ
HHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHH
VRRAYAVAESNRTVGRVLHELAQRALSVGKRVHSETAIDAAGASVVSVALGMAERKLGSL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AGTTAVVIGAGAMGALSAVHLTRAGVGHIQVLNRSLSRAQRLARRIRESGVPAEALALDR
HCCHHEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LANVLADADVVVSCTGAVRPVVSLADVHHALAAARRDEATRPLVICDLGMPRDVDPAVAR
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHH
LPCVWVVDVDSVQHEPSAHAAAADVEAARHIVAAEVASYLVGQRMAEVTPTVTALRQRAA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVEAELLRLDNRLPGLQSVQREEVARTVRRVVDKLLHAPTVRIKQLASAPGGDSYAEAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
RELFELDQTAVDAVATAGELPVVPSGFDAESRRGGGDMQSSPKRSPSN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVLLFGVSHRSAPVVVLEQLSIDESDQVKIIDRVLASPLVTEAMVLSTCNRVEVYAVVD
CEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
AFHGGLSVIGQVLAEHSGMSMGELTKYAYVRYSEAAVEHLFAVASGLDSAVIGEQQVLGQ
HHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHH
VRRAYAVAESNRTVGRVLHELAQRALSVGKRVHSETAIDAAGASVVSVALGMAERKLGSL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AGTTAVVIGAGAMGALSAVHLTRAGVGHIQVLNRSLSRAQRLARRIRESGVPAEALALDR
HCCHHEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LANVLADADVVVSCTGAVRPVVSLADVHHALAAARRDEATRPLVICDLGMPRDVDPAVAR
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHH
LPCVWVVDVDSVQHEPSAHAAAADVEAARHIVAAEVASYLVGQRMAEVTPTVTALRQRAA
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVEAELLRLDNRLPGLQSVQREEVARTVRRVVDKLLHAPTVRIKQLASAPGGDSYAEAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
RELFELDQTAVDAVATAGELPVVPSGFDAESRRGGGDMQSSPKRSPSN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972