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The map label for this gene is galE2 [C]

Identifier: 148660270

GI number: 148660270

Start: 592964

End: 594094

Strand: Direct

Name: galE2 [C]

Synonym: MRA_0509

Alternate gene names: 148660270

Gene position: 592964-594094 (Clockwise)

Preceding gene: 148660269

Following gene: 148660271

Centisome position: 13.42

GC content: 63.4

Gene sequence:

>1131_bases
GTGAGTTCGTCGAACGGGCGCGGTGGCGCCGGAGGAGTCGGCGGCAGCAGTGAGCACCCGCAGTACCCCAAAGTTGTGCT
GGTGACCGGTGCTTGCCGTTTCCTAGGCGGCTACCTGACCGCACGGCTTGCCCAGAACCCGCTGATCAACCGGGTCATCG
CGGTGGACGCGATCGCGCCGAGCAAGGACATGCTGCGCCGGATGGGCCGAGCCGAATTTGTTCGCGCTGATATCCGAAAC
CCATTCATCGCCAAGGTGATTCGCAATGGCGAGGTGGACACGGTGGTGCACGCCGCGGCGGCCTCGTATGCGCCGCGGTC
CGGCGGCAGTGCGGCATTGAAGGAACTTAACGTGATGGGCGCGATGCAACTGTTCGCCGCCTGCCAAAAGGCGCCCTCGG
TCCGCCGGGTCGTGCTGAAGTCGACCTCTGAGGTTTACGGATCGAGCCCACACGATCCGGTGATGTTCACCGAGGACAGC
AGCAGTCGACGTCCTTTCAGCCAAGGTTTCCCTAAGGACAGTCTCGATATCGAGGGCTACGTGCGCGCGCTGGGCCGACG
CCGCCCCGATATTGCAGTGACTATCCTGCGGCTGGCCAACATGATCGGCCCGGCGATGGACACCACGCTTTCACGATATC
TGGCCGGGCCGCTGGTCCCGACGATCTTCGGCCGTGATGCGCGACTGCAGTTGCTGCACGAGCAGGATGCGCTGGGTGCG
TTGGAGCGCGCGGCGATGGCCGGCAAGGCCGGAACGTTCAACATCGGAGCCGACGGCATCCTCATGCTGTCGCAGGCGAT
CCGGCGGGCCGGGCGAATTCCGGTGCCGGTGCCAGGGTTTGGGGTATGGGCTCTGGATTCGCTGAGGCGAGCGAATCACT
ACACCGAGCTGAATCGTGAGCAATTCGCTTACCTGAGTTATGGCCGGGTTATGGACACCACCAGAATGCGCGTCGAACTG
GGTTACCAGCCGAAGTGGACGACCGTCGAGGCGTTCGATGACTATTTTCGCGGCCGCGGCCTGACTCCCATTATTGACCC
ACATCGGGTACGCTCCTGGGAGGGTCGCGCCGTAGGTTTAGCGCAGCGCTGGGGTAGCCGAAATCCAATTCCATGGAGCG
GACTCAGATAG

Upstream 100 bases:

>100_bases
AGCGCAGGAAACTGGGCAAATAGGTTGCGAGCAGACCCCGCCAGCTCGACCGTCACGCGCTTGTAACGCCGCCGTTTCGC
CTGGCCGTTAGGCTGTCGGA

Downstream 100 bases:

>100_bases
GTTTGGATGGGTAACGTGGCGGGCGAAACCAGAGCGAATGTCATTCCACTGCACACAAATCGGAGCCGGGTAGCGGCGCG
CAGGCGTGCCGGTCAACGGG

Product: NAD-dependent epimerase/dehydratase family protein

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSSSNGRGGAGGVGGSSEHPQYPKVVLVTGACRFLGGYLTARLAQNPLINRVIAVDAIAPSKDMLRRMGRAEFVRADIRN
PFIAKVIRNGEVDTVVHAAAASYAPRSGGSAALKELNVMGAMQLFAACQKAPSVRRVVLKSTSEVYGSSPHDPVMFTEDS
SSRRPFSQGFPKDSLDIEGYVRALGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTIFGRDARLQLLHEQDALGA
LERAAMAGKAGTFNIGADGILMLSQAIRRAGRIPVPVPGFGVWALDSLRRANHYTELNREQFAYLSYGRVMDTTRMRVEL
GYQPKWTTVEAFDDYFRGRGLTPIIDPHRVRSWEGRAVGLAQRWGSRNPIPWSGLR

Sequences:

>Translated_376_residues
MSSSNGRGGAGGVGGSSEHPQYPKVVLVTGACRFLGGYLTARLAQNPLINRVIAVDAIAPSKDMLRRMGRAEFVRADIRN
PFIAKVIRNGEVDTVVHAAAASYAPRSGGSAALKELNVMGAMQLFAACQKAPSVRRVVLKSTSEVYGSSPHDPVMFTEDS
SSRRPFSQGFPKDSLDIEGYVRALGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTIFGRDARLQLLHEQDALGA
LERAAMAGKAGTFNIGADGILMLSQAIRRAGRIPVPVPGFGVWALDSLRRANHYTELNREQFAYLSYGRVMDTTRMRVEL
GYQPKWTTVEAFDDYFRGRGLTPIIDPHRVRSWEGRAVGLAQRWGSRNPIPWSGLR
>Mature_375_residues
SSSNGRGGAGGVGGSSEHPQYPKVVLVTGACRFLGGYLTARLAQNPLINRVIAVDAIAPSKDMLRRMGRAEFVRADIRNP
FIAKVIRNGEVDTVVHAAAASYAPRSGGSAALKELNVMGAMQLFAACQKAPSVRRVVLKSTSEVYGSSPHDPVMFTEDSS
SRRPFSQGFPKDSLDIEGYVRALGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTIFGRDARLQLLHEQDALGAL
ERAAMAGKAGTFNIGADGILMLSQAIRRAGRIPVPVPGFGVWALDSLRRANHYTELNREQFAYLSYGRVMDTTRMRVELG
YQPKWTTVEAFDDYFRGRGLTPIIDPHRVRSWEGRAVGLAQRWGSRNPIPWSGLR

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=292, Percent_Identity=27.0547945205479, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y501_MYCTU (P0A5D1)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   C70546
- PIR:   H70745
- RefSeq:   NP_215050.2
- RefSeq:   NP_334931.1
- ProteinModelPortal:   P0A5D1
- EnsemblBacteria:   EBMYCT00000002993
- EnsemblBacteria:   EBMYCT00000072795
- GeneID:   887228
- GeneID:   923918
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0522
- KEGG:   mtu:Rv0501
- TIGR:   MT0522
- TubercuList:   Rv0501
- GeneTree:   EBGT00050000015185
- HOGENOM:   HBG453006
- OMA:   EVEGYVR
- ProtClustDB:   CLSK881171
- GO:   GO:0005829
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01370 Epimerase

EC number: 5.1.3.2

Molecular weight: Translated: 41065; Mature: 40934

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSNGRGGAGGVGGSSEHPQYPKVVLVTGACRFLGGYLTARLAQNPLINRVIAVDAIAP
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCHHHHHHEEEECCCC
SKDMLRRMGRAEFVRADIRNPFIAKVIRNGEVDTVVHAAAASYAPRSGGSAALKELNVMG
CHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AMQLFAACQKAPSVRRVVLKSTSEVYGSSPHDPVMFTEDSSSRRPFSQGFPKDSLDIEGY
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCHHHH
VRALGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTIFGRDARLQLLHEQDALGA
HHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHH
LERAAMAGKAGTFNIGADGILMLSQAIRRAGRIPVPVPGFGVWALDSLRRANHYTELNRE
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHH
QFAYLSYGRVMDTTRMRVELGYQPKWTTVEAFDDYFRGRGLTPIIDPHRVRSWEGRAVGL
HHHHHHHCHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCEEEH
AQRWGSRNPIPWSGLR
HHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSSNGRGGAGGVGGSSEHPQYPKVVLVTGACRFLGGYLTARLAQNPLINRVIAVDAIAP
CCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCHHHHHHEEEECCCC
SKDMLRRMGRAEFVRADIRNPFIAKVIRNGEVDTVVHAAAASYAPRSGGSAALKELNVMG
CHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AMQLFAACQKAPSVRRVVLKSTSEVYGSSPHDPVMFTEDSSSRRPFSQGFPKDSLDIEGY
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCHHHH
VRALGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTIFGRDARLQLLHEQDALGA
HHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHH
LERAAMAGKAGTFNIGADGILMLSQAIRRAGRIPVPVPGFGVWALDSLRRANHYTELNRE
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHH
QFAYLSYGRVMDTTRMRVELGYQPKWTTVEAFDDYFRGRGLTPIIDPHRVRSWEGRAVGL
HHHHHHHCHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCEEEH
AQRWGSRNPIPWSGLR
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036