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The map label for this gene is mshA

Identifier: 148660253

GI number: 148660253

Start: 576658

End: 578100

Strand: Direct

Name: mshA

Synonym: MRA_0493

Alternate gene names: 148660253

Gene position: 576658-578100 (Clockwise)

Preceding gene: 148660252

Following gene: 148660254

Centisome position: 13.05

GC content: 70.2

Gene sequence:

>1443_bases
ATGGCAGGTGTGCGGCACGATGACGGTTCAGGGTTGATCGCCCAGCGCCGTCCGGTCCGCGGCGAGGGTGCCACCCGCTC
GCGCGGCCCATCCGGGCCATCCAATCGGAATGTTTCGGCAGCAGACGACCCGCGCCGGGTTGCGCTGCTGGCGGTGCACA
CCTCACCGCTGGCACAGCCGGGCACCGGTGACGCCGGCGGCATGAACGTCTACATGCTGCAAAGTGCGCTGCACCTGGCC
CGTCGGGGCATCGAGGTGGAGATCTTCACCCGGGCCACCGCATCGGCAGATCCACCGGTGGTGCGGGTGGCACCCGGGGT
GCTGGTGCGCAACGTGGTGGCGGGGCCCTTCGAGGGTTTGGACAAGTACGACCTGCCCACCCAGCTTTGTGCGTTCGCCG
CCGGGGTGCTGCGCGCCGAGGCGGTCCACGAACCGGGTTACTACGACATCGTGCACTCGCACTACTGGCTGTCGGGTCAG
GTCGGCTGGCTGGCGCGCGACCGCTGGGCGGTGCCGTTGGTGCACACCGCACACACGCTGGCCGCCGTGAAGAACGCGGC
ACTGGCCGACGGCGACGGACCCGAGCCGCCGCTGCGTACGGTCGGGGAGCAGCAGGTCGTCGACGAGGCGGATCGGTTGA
TCGTCAACACCGACGATGAAGCCAGGCAAGTGATTTCGCTTCATGGTGCCGATCCGGCACGAATCGACGTGGTCCATCCC
GGTGTCGATCTGGACGTGTTCCGCCCGGGTGATCGGCGCGCGGCCCGGGCCGCGCTAGGACTACCAGTTGACGAGCGCGT
GGTGGCCTTCGTCGGACGCATCCAGCCGCTGAAGGCACCCGACATTGTGCTGCGTGCGGCCGCCAAGTTGCCCGGGGTGC
GCATCATCGTGGCCGGCGGACCGTCGGGCAGCGGTCTGGCTTCACCGGACGGACTGGTCCGGCTCGCCGACGAACTGGGC
ATCTCTGCACGGGTGACGTTTCTGCCGCCGCAGTCCCACACGGATCTGGCCACCTTGTTTCGGGCGGCGGACCTGGTTGC
GGTGCCGAGCTACTCCGAGTCGTTCGGCCTGGTTGCTGTGGAGGCCCAAGCGTGCGGCACACCGGTGGTGGCCGCGGCGG
TGGGCGGGCTGCCCGTCGCGGTGCGCGACGGGATCACCGGCACCCTGGTGTCCGGGCACGAGGTCGGTCAGTGGGCCGAC
GCCATCGATCACCTGCTGCGGTTGTGTGCCGGGCCACGGGGACGGGTGATGAGCCGGGCGGCGGCACGGCACGCCGCCAC
GTTCTCGTGGGAGAACACCACCGACGCGCTGTTGGCCAGTTATCGGCGTGCGATCGGCGAGTACAACGCCGAGCGCCAGC
GCCGGGGCGGCGAGGTGATATCGGACCTGGTAGCGGTGGGCAAGCCCCGCCACTGGACGCCGCGTCGCGGGGTGGGCGCG
TGA

Upstream 100 bases:

>100_bases
CGGGCGCGCTGGATGCCCGGCTGCAGGACACCGCCCCGGAGGCGCTCGCGTGATCGGCTGACGAGCCGCGTCCGCGCGTG
TCACTTCGGTTCCTGCAAGG

Downstream 100 bases:

>100_bases
CTTCCTCCTTGCCGACCGTGCAACGTGTGATCCAGAATGCGCTCGAGGTCAGCCAGCTGAAGTACTCCCAACACCCCCGC
CCGGGCGGGGCGCCGCCCGC

Product: glycosyl transferase

Products: NA

Alternate protein names: N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase; GlcNAc-Ins-P N-acetylglucosaminyltransferase

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MAGVRHDDGSGLIAQRRPVRGEGATRSRGPSGPSNRNVSAADDPRRVALLAVHTSPLAQPGTGDAGGMNVYMLQSALHLA
RRGIEVEIFTRATASADPPVVRVAPGVLVRNVVAGPFEGLDKYDLPTQLCAFAAGVLRAEAVHEPGYYDIVHSHYWLSGQ
VGWLARDRWAVPLVHTAHTLAAVKNAALADGDGPEPPLRTVGEQQVVDEADRLIVNTDDEARQVISLHGADPARIDVVHP
GVDLDVFRPGDRRAARAALGLPVDERVVAFVGRIQPLKAPDIVLRAAAKLPGVRIIVAGGPSGSGLASPDGLVRLADELG
ISARVTFLPPQSHTDLATLFRAADLVAVPSYSESFGLVAVEAQACGTPVVAAAVGGLPVAVRDGITGTLVSGHEVGQWAD
AIDHLLRLCAGPRGRVMSRAAARHAATFSWENTTDALLASYRRAIGEYNAERQRRGGEVISDLVAVGKPRHWTPRRGVGA

Sequences:

>Translated_480_residues
MAGVRHDDGSGLIAQRRPVRGEGATRSRGPSGPSNRNVSAADDPRRVALLAVHTSPLAQPGTGDAGGMNVYMLQSALHLA
RRGIEVEIFTRATASADPPVVRVAPGVLVRNVVAGPFEGLDKYDLPTQLCAFAAGVLRAEAVHEPGYYDIVHSHYWLSGQ
VGWLARDRWAVPLVHTAHTLAAVKNAALADGDGPEPPLRTVGEQQVVDEADRLIVNTDDEARQVISLHGADPARIDVVHP
GVDLDVFRPGDRRAARAALGLPVDERVVAFVGRIQPLKAPDIVLRAAAKLPGVRIIVAGGPSGSGLASPDGLVRLADELG
ISARVTFLPPQSHTDLATLFRAADLVAVPSYSESFGLVAVEAQACGTPVVAAAVGGLPVAVRDGITGTLVSGHEVGQWAD
AIDHLLRLCAGPRGRVMSRAAARHAATFSWENTTDALLASYRRAIGEYNAERQRRGGEVISDLVAVGKPRHWTPRRGVGA
>Mature_479_residues
AGVRHDDGSGLIAQRRPVRGEGATRSRGPSGPSNRNVSAADDPRRVALLAVHTSPLAQPGTGDAGGMNVYMLQSALHLAR
RGIEVEIFTRATASADPPVVRVAPGVLVRNVVAGPFEGLDKYDLPTQLCAFAAGVLRAEAVHEPGYYDIVHSHYWLSGQV
GWLARDRWAVPLVHTAHTLAAVKNAALADGDGPEPPLRTVGEQQVVDEADRLIVNTDDEARQVISLHGADPARIDVVHPG
VDLDVFRPGDRRAARAALGLPVDERVVAFVGRIQPLKAPDIVLRAAAKLPGVRIIVAGGPSGSGLASPDGLVRLADELGI
SARVTFLPPQSHTDLATLFRAADLVAVPSYSESFGLVAVEAQACGTPVVAAAVGGLPVAVRDGITGTLVSGHEVGQWADA
IDHLLRLCAGPRGRVMSRAAARHAATFSWENTTDALLASYRRAIGEYNAERQRRGGEVISDLVAVGKPRHWTPRRGVGA

Specific function: Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2- acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family. MshA subfamily

Homologues:

Organism=Escherichia coli, GI1790061, Length=192, Percent_Identity=26.5625, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHA_MYCBO (P64708)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854159.1
- ProteinModelPortal:   P64708
- SMR:   P64708
- EnsemblBacteria:   EBMYCT00000017595
- GeneID:   1091537
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0496
- GeneTree:   EBGT00050000015521
- HOGENOM:   HBG726846
- OMA:   PLKAPDV
- ProtClustDB:   CLSK871811
- BioCyc:   MBOV233413:MB0496-MONOMER
- HAMAP:   MF_01695
- InterPro:   IPR001296
- InterPro:   IPR017814
- TIGRFAMs:   TIGR03449

Pfam domain/function: PF00534 Glycos_transf_1

EC number: =2.4.1.250

Molecular weight: Translated: 50542; Mature: 50411

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00039 DEAD_ATP_HELICASE ; PS00584 PFKB_KINASES_2

Important sites: BINDING 53-53 BINDING 67-67 BINDING 122-122 BINDING 155-155 BINDING 179-179 BINDING 199-199 BINDING 273-273 BINDING 278-278 BINDING 331-331 BINDING 353-353 BINDING 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVRHDDGSGLIAQRRPVRGEGATRSRGPSGPSNRNVSAADDPRRVALLAVHTSPLAQP
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
GTGDAGGMNVYMLQSALHLARRGIEVEIFTRATASADPPVVRVAPGVLVRNVVAGPFEGL
CCCCCCCEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCCCC
DKYDLPTQLCAFAAGVLRAEAVHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTL
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEECCCCCEEECCCCCCCHHHHHHHH
AAVKNAALADGDGPEPPLRTVGEQQVVDEADRLIVNTDDEARQVISLHGADPARIDVVHP
HHHHHHHCCCCCCCCCHHHHCCHHHHHHHHCCEEECCCHHHHHHHEECCCCCCEEEEECC
GVDLDVFRPGDRRAARAALGLPVDERVVAFVGRIQPLKAPDIVLRAAAKLPGVRIIVAGG
CCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEC
PSGSGLASPDGLVRLADELGISARVTFLPPQSHTDLATLFRAADLVAVPSYSESFGLVAV
CCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCEEEECCCCCCCCEEEE
EAQACGTPVVAAAVGGLPVAVRDGITGTLVSGHEVGQWADAIDHLLRLCAGPRGRVMSRA
EHHHCCCCHHHHHHCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
AARHAATFSWENTTDALLASYRRAIGEYNAERQRRGGEVISDLVAVGKPRHWTPRRGVGA
HHHHHEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGVRHDDGSGLIAQRRPVRGEGATRSRGPSGPSNRNVSAADDPRRVALLAVHTSPLAQP
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
GTGDAGGMNVYMLQSALHLARRGIEVEIFTRATASADPPVVRVAPGVLVRNVVAGPFEGL
CCCCCCCEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCCCC
DKYDLPTQLCAFAAGVLRAEAVHEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTL
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEECCCCCEEECCCCCCCHHHHHHHH
AAVKNAALADGDGPEPPLRTVGEQQVVDEADRLIVNTDDEARQVISLHGADPARIDVVHP
HHHHHHHCCCCCCCCCHHHHCCHHHHHHHHCCEEECCCHHHHHHHEECCCCCCEEEEECC
GVDLDVFRPGDRRAARAALGLPVDERVVAFVGRIQPLKAPDIVLRAAAKLPGVRIIVAGG
CCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEC
PSGSGLASPDGLVRLADELGISARVTFLPPQSHTDLATLFRAADLVAVPSYSESFGLVAV
CCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCEEEECCCCCCCCEEEE
EAQACGTPVVAAAVGGLPVAVRDGITGTLVSGHEVGQWADAIDHLLRLCAGPRGRVMSRA
EHHHCCCCHHHHHHCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
AARHAATFSWENTTDALLASYRRAIGEYNAERQRRGGEVISDLVAVGKPRHWTPRRGVGA
HHHHHEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972