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The map label for this gene is ptrB [C]

Identifier: 148660223

GI number: 148660223

Start: 548896

End: 550917

Strand: Reverse

Name: ptrB [C]

Synonym: MRA_0463

Alternate gene names: 148660223

Gene position: 550917-548896 (Counterclockwise)

Preceding gene: 148660230

Following gene: 148660222

Centisome position: 12.46

GC content: 64.29

Gene sequence:

>2022_bases
ATGACATTTGAGCCTGCCCCAGACGGCGCTGACCCCTACCTATGGCTCGAGGACGTCACCGGTGCGGAAGCGCTGGATTG
GGTACGCGCGCGCAACAAGCCGACAACGGCGGCGTTCTGCGACGCAGAGTTCGAGCGGATGCGCGTCGAGGCGCTCGAGG
TGCTCGACACCGATGCCCGAATCCCCTATGTGAACAGGCGCGGCAACTATCTCTACAACTTCTGGCGCGATGCCGCCAAC
CCCCGCGGGTTGTGGCGGCGCACCACGCTGGACAGCTACCGCACCGACTCGCCGGGCTGGGATGTGCTGATCGATGTTGA
TGAACTGGGCCGCGCCGACGACCAGAAGTGGGTGTGGGGCGGCGCCGGTGTCATCGAACCCGACTACACGCGCGCGCTGA
TCGGCCTGTCCCCGGGCGGCTCGGATGCATCCATTGTGCGTGAATTCGACATGCTGACACGCGAATTCGTCGAGGATGGG
TTCCAGCTGCCACCGGCCAAATCCCAGATTACCTGGGAGGACCCGGACACTGTCTTGCTGGGCACCGACTTCGGCGGCGA
CTCGCTCACCACTTCCGGCTATCCGCGAGTGATCAAGCGATGGCGCCGGGGCAAACCGCTGGCCGATGCGGAGACAATCT
TCGAGGGCGCGGGCACCGACGTCCGCGTCAACGCGTCAGCGGATCGCACGCCCGGATTCGAGCGCACCCTGCTGGGACGG
GCCCTCGACTTCTGGAACGAAGAGGTCTACGAATTGCGTGGTTCGGAGCTGATCCGAATCGAAGCCCCCACCGACGCGAG
CGTGTCAATTCACCGCGACTGGCTGTTGATCGAGCTGCGCACCGACTGGACCGTGGCCACCACCAGGTACACTGCCGGCT
CACTGCTGGCCGCCGAATACGACGAATTCCTTGCCGGCTCAGCGGAATTACAGGTGGTGTTCGAACCCGACGAGCACACC
GCGCTGTACCAATACGCCTGGACCCGAGACCGGCTGTTGATCGTCACCCTGGCCGACGTGGCGAGCCGCGTCGAGATCGC
AACTCCCGGCAGCTGGCGACGTGAGCCACTCTCAGGTATCCCCGCCGCCACCAACACCGTCATCGTCTCCGCCGACAGCC
ACGGCGACGAGTTCTTCCTCGACTCCAGTGGATTCGATACGCCGTCGCGGCTGATGCGCGGCACCGATGACGGCCGACTA
GCAGAGATCAAATCTGCGCCAGCTTTCTTCGACGCGGAAAACATGGCTGTAACACAGTATTTCGCGACATCAGACGATGG
CACATCGATCCCGTATTTCGTCGTGCGACGCACCGACGCGGACAATCCCGGCCCCACATTGTTGAACGGCTACGGTGGAT
TCGAGACATCCAGAACGCCGACGTATGACGGCGTCCTGGGCCGGCTGTGGCTGGCCCGCGGGGGCACCTATGCGTTGGCC
AACATCCGCGGCGGCGGCGAGTACGGGCCTGGCTGGCACACGCAGGCGATGCGCGAGGGCCGGGACAAGGTCGCCCAAGA
CTTCGCCGCCGTGGCAACCGATTTGGTGACCCGCGGCATCACCACCGCTGAGCAGCTCGGCGCGCGGGGCGGCAGCAACG
GTGGGCTGCTGATGGGCATCATGTTGACCGGGTACCCGGAGAAGTTCGGCGCGCTGGTCTGCGACGTGCCGCTGCTGGAC
ATGAAGCGGTACCACCTGCTGCTGGCCGGCGCTTCCTGGATGGCCGAATACGGCGATCCGGATAACCCGGATGACTGGAA
GTTCATCTCTGAATATTCGCCATACCAAAACATTTCGGCGAACCGGAAATACCCGCCAGTACTCATGACGACCTCTACCC
GCGACGATCGGGTGCACCCCGGCCACGCCCGCAAAATGACGGCGGCCCTGCAGGCTGCGGGCCACCCCGTCTGGTATTAC
GAAAACATCGAGGGTGGGCACGCCGGCGCAGCCGACAACGCACAGATCGCATTCAAATCGGCGCTGAGTTTCGCGTTCCT
ATGGCGGATGCTGGCCGGCTAA

Upstream 100 bases:

>100_bases
CCCGGCGGAACCGGGACGGGAAAAGACAGTCATCGTATTCGGCTCCTCGTCAAAATCATGTAATACAGCTCACACTACCG
CCGGGGCCACAATGGCTTCC

Downstream 100 bases:

>100_bases
CCGGCATTCACGAACGGTGGACTTAGCCCAAATAACGTGGCTATCGAGTTGTAGCCGATTGATACTGCATTACTACCTAT
TACTACGTATTACTATCTGT

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDARIPYVNRRGNYLYNFWRDAAN
PRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDG
FQLPPAKSQITWEDPDTVLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTLLGR
ALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEYDEFLAGSAELQVVFEPDEHT
ALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRL
AEIKSAPAFFDAENMAVTQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTYALA
NIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGIMLTGYPEKFGALVCDVPLLD
MKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYY
ENIEGGHAGAADNAQIAFKSALSFAFLWRMLAG

Sequences:

>Translated_673_residues
MTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDARIPYVNRRGNYLYNFWRDAAN
PRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDG
FQLPPAKSQITWEDPDTVLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTLLGR
ALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEYDEFLAGSAELQVVFEPDEHT
ALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRL
AEIKSAPAFFDAENMAVTQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTYALA
NIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGIMLTGYPEKFGALVCDVPLLD
MKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYY
ENIEGGHAGAADNAQIAFKSALSFAFLWRMLAG
>Mature_672_residues
TFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDARIPYVNRRGNYLYNFWRDAANP
RGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWGGAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDGF
QLPPAKSQITWEDPDTVLLGTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTLLGRA
LDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEYDEFLAGSAELQVVFEPDEHTA
LYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGIPAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRLA
EIKSAPAFFDAENMAVTQYFATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTYALAN
IRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGIMLTGYPEKFGALVCDVPLLDM
KRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISANRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYYE
NIEGGHAGAADNAQIAFKSALSFAFLWRMLAG

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=388, Percent_Identity=30.1546391752577, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI284172438, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI284172431, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI284172420, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI284172413, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI70778815, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI108860686, Length=203, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI108860692, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788150, Length=299, Percent_Identity=28.4280936454849, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583414, Length=318, Percent_Identity=36.1635220125786, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI221510989, Length=240, Percent_Identity=39.5833333333333, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 74465; Mature: 74333

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDAR
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCC
IPYVNRRGNYLYNFWRDAANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWG
CCEEECCCCEEHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCEEEC
GAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDGFQLPPAKSQITWEDPDTVLL
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEE
GTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTLLGR
ECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
ALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEY
HHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCEEEEEEECCEEEEEEEECCCCHHHHHH
DEFLAGSAELQVVFEPDEHTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGI
HHHHCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECHHHCEEEEECCCCCCCCCCCCC
PAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRLAEIKSAPAFFDAENMAVTQY
CCCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCCEEEECCCCCEECCCCEEEEEE
FATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTYALA
EEECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
NIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGI
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEE
MLTGYPEKFGALVCDVPLLDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISA
EEECCCHHHCCEEECCCHHCCCEEEEEEECHHHHHHCCCCCCCCCCHHHHCCCCCCCCCC
NRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYYENIEGGHAGAADNAQIAFKS
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHH
ALSFAFLWRMLAG
HHHHHHHHHHHCC
>Mature Secondary Structure 
TFEPAPDGADPYLWLEDVTGAEALDWVRARNKPTTAAFCDAEFERMRVEALEVLDTDAR
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCC
IPYVNRRGNYLYNFWRDAANPRGLWRRTTLDSYRTDSPGWDVLIDVDELGRADDQKWVWG
CCEEECCCCEEHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCEEEC
GAGVIEPDYTRALIGLSPGGSDASIVREFDMLTREFVEDGFQLPPAKSQITWEDPDTVLL
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEE
GTDFGGDSLTTSGYPRVIKRWRRGKPLADAETIFEGAGTDVRVNASADRTPGFERTLLGR
ECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
ALDFWNEEVYELRGSELIRIEAPTDASVSIHRDWLLIELRTDWTVATTRYTAGSLLAAEY
HHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCEEEEEEECCEEEEEEEECCCCHHHHHH
DEFLAGSAELQVVFEPDEHTALYQYAWTRDRLLIVTLADVASRVEIATPGSWRREPLSGI
HHHHCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEECHHHCEEEEECCCCCCCCCCCCC
PAATNTVIVSADSHGDEFFLDSSGFDTPSRLMRGTDDGRLAEIKSAPAFFDAENMAVTQY
CCCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCCEEEECCCCCEECCCCEEEEEE
FATSDDGTSIPYFVVRRTDADNPGPTLLNGYGGFETSRTPTYDGVLGRLWLARGGTYALA
EEECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
NIRGGGEYGPGWHTQAMREGRDKVAQDFAAVATDLVTRGITTAEQLGARGGSNGGLLMGI
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEE
MLTGYPEKFGALVCDVPLLDMKRYHLLLAGASWMAEYGDPDNPDDWKFISEYSPYQNISA
EEECCCHHHCCEEECCCHHCCCEEEEEEECHHHHHHCCCCCCCCCCHHHHCCCCCCCCCC
NRKYPPVLMTTSTRDDRVHPGHARKMTAALQAAGHPVWYYENIEGGHAGAADNAQIAFKS
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHH
ALSFAFLWRMLAG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]