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The map label for this gene is moeA2

Identifier: 148660203

GI number: 148660203

Start: 527453

End: 528670

Strand: Reverse

Name: moeA2

Synonym: MRA_0443

Alternate gene names: 148660203

Gene position: 528670-527453 (Counterclockwise)

Preceding gene: 148660204

Following gene: 148660202

Centisome position: 11.96

GC content: 67.73

Gene sequence:

>1218_bases
GTGCGGTCAGTCCAGGAGCATCAACGTGTCGTAGCGGAGATGATGCGCGCCTGTCGCCCGATTACGGTTCCGCTGACCCA
GGCTCAAGGTCTGGTCCTGGGCGGCGACGTGGTCGCACCGCTGTCGCTGCCGGTTTTCGACAACTCCGCAATGGATGGCT
ATGCGGTGCGCGCCGAAGACACCTCAGGTGCCACACCGCAAAATCCGGTGATGTTGCCGGTCGCCGAGGACATTCCCGCC
GGGCGCGCCGACATGCTGACGCTGCAGCCTGTGACTGCGCACCGGATCATGACCGGTGCGCCAGTGCCCACCGGTGCGAC
GGCGATCGTGCCGGTCGAAGCCACCGACGGCGGCGTCGATTCGGTGGCGATCCGCCAACAGGCCACCCCAGGCAAGCACA
TCCGACGGTCGGGCGAAGACGTCGCCGCCGGTACCACGGTGCTGCACAACGGCCAGATCGTGACCCCGGCGGTGCTCGGC
CTGGCCGCCGCGCTGGGTTTGGCCGAGCTGCCGGTGCTCCCCCGTCAGCGGGTGCTGGTGATCTCCACAGGGTCGGAGCT
GGCGTCGCCAGGCACGCCCCTACAACCGGGTCAGATCTACGAGTCCAACTCGATCATGCTGGCCGCTGCCGTCCGCGATG
CGGGCGCCGCTGTGGTCGCCACCGCAACCGCCGGCGACGACGTCGCGCAGTTCGGCGCGATCCTTGACCGGTACGCGGTG
GACGCCGACCTGATCATCACCAGCGGCGGTGTCAGTGCCGGAGCCTACGAGGTGGTCAAAGACGCGTTCGGCAGCGCTGA
CTACCGGGGAGGCGACCACGGTGTCGAATTCGTCAAGGTGGCGATGCAACCCGGAATGCCCCAGGGCGTCGGGCGGGTGG
CAGGTACGCCGATCGTCACCCTTCCCGGCAACCCGGTCAGCGCGCTGGTGTCCTTCGAGGTGTTCATCCGTCCCCCGCTG
CGCATGGCCATGGGCCTGCCGGATCCGTACCGGCCGCACCGAAGCGCGGTACTCACCGCGAGCCTGACATCGCCGCGCGG
CAAACGTCAGTTCCGACGCGCAATACTCGATCACCAGGCAGGCACGGTCATCAGCTACGGCCCACCGGCGTCGCACCATT
TGCGTTGGTTGGCATCGGCCAACGGACTTCTGGACATCCCGGAGGACGTCGTGGAGGTGGCTGCCGGAACGCAGCTGCAA
GTCTGGGACTTGACCTAA

Upstream 100 bases:

>100_bases
CGCGCAGTCCCACGACAAAGATCTGCAGCGCCGCCTCTGGACCGTCTCCGAAGAACTCACCGGCGTCAGCTTCGGAGTCT
GAACGACCGATAATGGCCGG

Downstream 100 bases:

>100_bases
CCGGACCGATCCTAGTGCCGTGCACCCAACCTGCGGCACGAAGCTCCGTAAGATGACCCCGTGGCCAGACGCCCCCGCCC
CGACGGCCCGCAACATCTGC

Product: molybdopterin biosynthesis protein MoeA2

Products: NA

Alternate protein names: MPT Mo-transferase 2

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MRSVQEHQRVVAEMMRACRPITVPLTQAQGLVLGGDVVAPLSLPVFDNSAMDGYAVRAEDTSGATPQNPVMLPVAEDIPA
GRADMLTLQPVTAHRIMTGAPVPTGATAIVPVEATDGGVDSVAIRQQATPGKHIRRSGEDVAAGTTVLHNGQIVTPAVLG
LAAALGLAELPVLPRQRVLVISTGSELASPGTPLQPGQIYESNSIMLAAAVRDAGAAVVATATAGDDVAQFGAILDRYAV
DADLIITSGGVSAGAYEVVKDAFGSADYRGGDHGVEFVKVAMQPGMPQGVGRVAGTPIVTLPGNPVSALVSFEVFIRPPL
RMAMGLPDPYRPHRSAVLTASLTSPRGKRQFRRAILDHQAGTVISYGPPASHHLRWLASANGLLDIPEDVVEVAAGTQLQ
VWDLT

Sequences:

>Translated_405_residues
MRSVQEHQRVVAEMMRACRPITVPLTQAQGLVLGGDVVAPLSLPVFDNSAMDGYAVRAEDTSGATPQNPVMLPVAEDIPA
GRADMLTLQPVTAHRIMTGAPVPTGATAIVPVEATDGGVDSVAIRQQATPGKHIRRSGEDVAAGTTVLHNGQIVTPAVLG
LAAALGLAELPVLPRQRVLVISTGSELASPGTPLQPGQIYESNSIMLAAAVRDAGAAVVATATAGDDVAQFGAILDRYAV
DADLIITSGGVSAGAYEVVKDAFGSADYRGGDHGVEFVKVAMQPGMPQGVGRVAGTPIVTLPGNPVSALVSFEVFIRPPL
RMAMGLPDPYRPHRSAVLTASLTSPRGKRQFRRAILDHQAGTVISYGPPASHHLRWLASANGLLDIPEDVVEVAAGTQLQ
VWDLT
>Mature_405_residues
MRSVQEHQRVVAEMMRACRPITVPLTQAQGLVLGGDVVAPLSLPVFDNSAMDGYAVRAEDTSGATPQNPVMLPVAEDIPA
GRADMLTLQPVTAHRIMTGAPVPTGATAIVPVEATDGGVDSVAIRQQATPGKHIRRSGEDVAAGTTVLHNGQIVTPAVLG
LAAALGLAELPVLPRQRVLVISTGSELASPGTPLQPGQIYESNSIMLAAAVRDAGAAVVATATAGDDVAQFGAILDRYAV
DADLIITSGGVSAGAYEVVKDAFGSADYRGGDHGVEFVKVAMQPGMPQGVGRVAGTPIVTLPGNPVSALVSFEVFIRPPL
RMAMGLPDPYRPHRSAVLTASLTSPRGKRQFRRAILDHQAGTVISYGPPASHHLRWLASANGLLDIPEDVVEVAAGTQLQ
VWDLT

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family

Homologues:

Organism=Homo sapiens, GI10880983, Length=400, Percent_Identity=36, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI66932975, Length=400, Percent_Identity=36, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1787049, Length=387, Percent_Identity=36.1757105943152, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI25147659, Length=391, Percent_Identity=28.6445012787724, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17562606, Length=354, Percent_Identity=24.8587570621469, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136978, Length=369, Percent_Identity=28.9972899728997, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24638841, Length=369, Percent_Identity=28.9972899728997, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOEA2_MYCTU (O53725)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   G70829
- RefSeq:   NP_334863.1
- RefSeq:   YP_177725.1
- ProteinModelPortal:   O53725
- SMR:   O53725
- EnsemblBacteria:   EBMYCT00000000826
- EnsemblBacteria:   EBMYCT00000070616
- GeneID:   886348
- GeneID:   923772
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0454
- KEGG:   mtu:Rv0438c
- TIGR:   MT0454
- TubercuList:   Rv0438c
- GeneTree:   EBGT00050000016425
- HOGENOM:   HBG731522
- OMA:   SPKVESH
- ProtClustDB:   CLSK799709
- InterPro:   IPR020817
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453
- Gene3D:   G3DSA:3.40.980.10
- SMART:   SM00852
- TIGRFAMs:   TIGR00177

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N; SSF53218 MoCF_biosynth; SSF63867 MoeA_C; SSF63882 MoeA_N

EC number: NA

Molecular weight: Translated: 42022; Mature: 42022

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVQEHQRVVAEMMRACRPITVPLTQAQGLVLGGDVVAPLSLPVFDNSAMDGYAVRAED
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEECEEEEEECCCCCCCEEEEECC
TSGATPQNPVMLPVAEDIPAGRADMLTLQPVTAHRIMTGAPVPTGATAIVPVEATDGGVD
CCCCCCCCCEEEEECCCCCCCCCCEEEECCCHHHHEEECCCCCCCCEEEEEEECCCCCCC
SVAIRQQATPGKHIRRSGEDVAAGTTVLHNGQIVTPAVLGLAAALGLAELPVLPRQRVLV
CEEEECCCCCCHHHHCCCCCCCCCEEEEECCEECHHHHHHHHHHHHHHHCCCCCCCCEEE
ISTGSELASPGTPLQPGQIYESNSIMLAAAVRDAGAAVVATATAGDDVAQFGAILDRYAV
EECCCHHCCCCCCCCCCEEECCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHC
DADLIITSGGVSAGAYEVVKDAFGSADYRGGDHGVEFVKVAMQPGMPQGVGRVAGTPIVT
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCEEE
LPGNPVSALVSFEVFIRPPLRMAMGLPDPYRPHRSAVLTASLTSPRGKRQFRRAILDHQA
ECCCCCEEEEEEEEEECCCHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
GTVISYGPPASHHLRWLASANGLLDIPEDVVEVAAGTQLQVWDLT
CCEEECCCCHHHHEEEEECCCCCCCCCHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MRSVQEHQRVVAEMMRACRPITVPLTQAQGLVLGGDVVAPLSLPVFDNSAMDGYAVRAED
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCEEECEEEEEECCCCCCCEEEEECC
TSGATPQNPVMLPVAEDIPAGRADMLTLQPVTAHRIMTGAPVPTGATAIVPVEATDGGVD
CCCCCCCCCEEEEECCCCCCCCCCEEEECCCHHHHEEECCCCCCCCEEEEEEECCCCCCC
SVAIRQQATPGKHIRRSGEDVAAGTTVLHNGQIVTPAVLGLAAALGLAELPVLPRQRVLV
CEEEECCCCCCHHHHCCCCCCCCCEEEEECCEECHHHHHHHHHHHHHHHCCCCCCCCEEE
ISTGSELASPGTPLQPGQIYESNSIMLAAAVRDAGAAVVATATAGDDVAQFGAILDRYAV
EECCCHHCCCCCCCCCCEEECCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHC
DADLIITSGGVSAGAYEVVKDAFGSADYRGGDHGVEFVKVAMQPGMPQGVGRVAGTPIVT
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCEEE
LPGNPVSALVSFEVFIRPPLRMAMGLPDPYRPHRSAVLTASLTSPRGKRQFRRAILDHQA
ECCCCCEEEEEEEEEECCCHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
GTVISYGPPASHHLRWLASANGLLDIPEDVVEVAAGTQLQVWDLT
CCEEECCCCHHHHEEEEECCCCCCCCCHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036