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The map label for this gene is thiO [H]

Identifier: 148660180

GI number: 148660180

Start: 502458

End: 503480

Strand: Direct

Name: thiO [H]

Synonym: MRA_0421

Alternate gene names: 148660180

Gene position: 502458-503480 (Clockwise)

Preceding gene: 148660178

Following gene: 148660181

Centisome position: 11.37

GC content: 69.01

Gene sequence:

>1023_bases
ATGGCGTCCGACCTACACACCGGGTCGCTGGCTGTCATCGGCGGCGGTGTCATCGGGCTGTCGGTGGCCCGCCGTGCCGC
CCAAGCCGGCTGGCCGGTGCGGGTGCACCGCAGCGACGAGCGGGGGGCGTCCTGGGTTGCCGGCGGCATGCTGGCCCCAC
ACAGCGAAGGCTGGCCCGGCGAGGAACGGTTGTTGCGGCTAGGCCTGCAGTCCCTGCGGCTTTGGCGTGAGGGCAGCTTT
CTCGACGGGCTGGGCCCGCAACTGGTCACCGCGCACGAGTCGCTGGTGGTGGCCGTCGACCGGGCCGACGTCGCCGACCT
GCGCACTGTCGCGGACTGGTTGTCCGCACAGGGGCACCCGGTGATCTGGGAGTCGGCTGCCCGTGACGTCGAACCCCTAC
TGGCGCAAGGCATCCGGCACGGGTTTCGGGCGCCCACCGAACTGGCCGTCGACAACCGCGCCCTGCTCGACGCGCTGTGC
CGTGACTGCGAGCGACTCGGAGTTCGCTGGAGCTCACAGGTGAGCAGCCTGTCCGACGTCGATGCGCACACGGTGGTGAT
CGCCAACGGCATTGACGCCCCGGCCTTGTGGCCCGGCCTGCCGATACGCCCGGTGAAGGGTGAGGTGCTGCGGCTGCGAT
GGCGACCAGGTTGTATGCCCTTGCCGCAGAGAGTGATTCGTGCCCGTGTGCGTGGACGACAGGTCTATCTGGTGCCACGT
TCGGACGGGGTGGTCGTCGGCGCCACCCAATACGAGCACGGGCGCGACACCGCGCCGGTGGTATCGGGAGTTCGTGACCT
GCTAGACGATGCGTGTACCGTGCTGCCGGCGCTGGGTGAGTACGAGCTGGCCGAGTGTGAGGCCGGACTGCGCCCGATGA
CACCCGACAACTTGCCGCTGGTCCAACGCCTGGATTCGCGGACCCTGGTCGCGGCCGGTCACGGCCGATCCGGATTCCTA
TTGGCGCCGTGGACTGCCGAACAGATTGTGTCCGAACTCGTTTCGGTTGGGGCCGCCTCATGA

Upstream 100 bases:

>100_bases
CCTGTAGACACGGGAGTCCCGGGAGCGGGGTCTGAGAGTGGGCGCGCCTGCCCTTACCGTCACACCTGATCCGGATCATG
CCGGCGAAGGGAGGTCAAGG

Downstream 100 bases:

>100_bases
TCGTCGTTGTCAACGAGCAACAGGTCGAGGTCGACGAGCAGACCACCATCGCCGCGCTGCTGGATTCGCTGGGCTTCGGG
GACCGGGGTATCGCTGTGGC

Product: putative amino acid oxidase flavoprotein ThiO

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MASDLHTGSLAVIGGGVIGLSVARRAAQAGWPVRVHRSDERGASWVAGGMLAPHSEGWPGEERLLRLGLQSLRLWREGSF
LDGLGPQLVTAHESLVVAVDRADVADLRTVADWLSAQGHPVIWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLDALC
RDCERLGVRWSSQVSSLSDVDAHTVVIANGIDAPALWPGLPIRPVKGEVLRLRWRPGCMPLPQRVIRARVRGRQVYLVPR
SDGVVVGATQYEHGRDTAPVVSGVRDLLDDACTVLPALGEYELAECEAGLRPMTPDNLPLVQRLDSRTLVAAGHGRSGFL
LAPWTAEQIVSELVSVGAAS

Sequences:

>Translated_340_residues
MASDLHTGSLAVIGGGVIGLSVARRAAQAGWPVRVHRSDERGASWVAGGMLAPHSEGWPGEERLLRLGLQSLRLWREGSF
LDGLGPQLVTAHESLVVAVDRADVADLRTVADWLSAQGHPVIWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLDALC
RDCERLGVRWSSQVSSLSDVDAHTVVIANGIDAPALWPGLPIRPVKGEVLRLRWRPGCMPLPQRVIRARVRGRQVYLVPR
SDGVVVGATQYEHGRDTAPVVSGVRDLLDDACTVLPALGEYELAECEAGLRPMTPDNLPLVQRLDSRTLVAAGHGRSGFL
LAPWTAEQIVSELVSVGAAS
>Mature_339_residues
ASDLHTGSLAVIGGGVIGLSVARRAAQAGWPVRVHRSDERGASWVAGGMLAPHSEGWPGEERLLRLGLQSLRLWREGSFL
DGLGPQLVTAHESLVVAVDRADVADLRTVADWLSAQGHPVIWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLDALCR
DCERLGVRWSSQVSSLSDVDAHTVVIANGIDAPALWPGLPIRPVKGEVLRLRWRPGCMPLPQRVIRARVRGRQVYLVPRS
DGVVVGATQYEHGRDTAPVVSGVRDLLDDACTVLPALGEYELAECEAGLRPMTPDNLPLVQRLDSRTLVAAGHGRSGFLL
APWTAEQIVSELVSVGAAS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 36535; Mature: 36404

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDLHTGSLAVIGGGVIGLSVARRAAQAGWPVRVHRSDERGASWVAGGMLAPHSEGWPG
CCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCC
EERLLRLGLQSLRLWREGSFLDGLGPQLVTAHESLVVAVDRADVADLRTVADWLSAQGHP
HHHHHHHHHHHHHHHHCCCCCCCCCCHHEECCCEEEEEECCCCHHHHHHHHHHHCCCCCC
VIWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLDALCRDCERLGVRWSSQVSSLSDV
EEEHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DAHTVVIANGIDAPALWPGLPIRPVKGEVLRLRWRPGCMPLPQRVIRARVRGRQVYLVPR
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEC
SDGVVVGATQYEHGRDTAPVVSGVRDLLDDACTVLPALGEYELAECEAGLRPMTPDNLPL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHH
VQRLDSRTLVAAGHGRSGFLLAPWTAEQIVSELVSVGAAS
HHHCCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASDLHTGSLAVIGGGVIGLSVARRAAQAGWPVRVHRSDERGASWVAGGMLAPHSEGWPG
CCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCC
EERLLRLGLQSLRLWREGSFLDGLGPQLVTAHESLVVAVDRADVADLRTVADWLSAQGHP
HHHHHHHHHHHHHHHHCCCCCCCCCCHHEECCCEEEEEECCCCHHHHHHHHHHHCCCCCC
VIWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLDALCRDCERLGVRWSSQVSSLSDV
EEEHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DAHTVVIANGIDAPALWPGLPIRPVKGEVLRLRWRPGCMPLPQRVIRARVRGRQVYLVPR
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEC
SDGVVVGATQYEHGRDTAPVVSGVRDLLDDACTVLPALGEYELAECEAGLRPMTPDNLPL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHH
VQRLDSRTLVAAGHGRSGFLLAPWTAEQIVSELVSVGAAS
HHHCCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]