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The map label for this gene is fadD30 [H]

Identifier: 148660169

GI number: 148660169

Start: 485287

End: 487044

Strand: Direct

Name: fadD30 [H]

Synonym: MRA_0410

Alternate gene names: 148660169

Gene position: 485287-487044 (Clockwise)

Preceding gene: 148660166

Following gene: 148660170

Centisome position: 10.98

GC content: 53.87

Gene sequence:

>1758_bases
ATGTCTGTTATATCCACGTTGCGCGACCGTGCGACCACGACTCCAAGCGACGAAGCCTTTGTGTTCATGGATTACGACAC
AAAAACCGGCGACCAAATTGACCGAATGACGTGGAGTCAATTATATTCTCGCGTCACCGCCGTGTCTGCGTATCTAATAA
GTTATGGCCGGCATGCTGACCGACGAAGGACCGCAGCGATATCAGCTCCGCAAGGTCTGGACTATGTTGCAGGATTTCTA
GGAGCACTGTGCGCCGGATGGACGCCGGTTCCGTTACCAGAACCGCTGGGCAGCCTACGCGATAAGCGGACTGGACTGGC
TGTACTCGACTGTGCCGCCGACGTCGTGCTGACGACGTCGCAAGCCGAAACGCGGGTCAGGGCCACGATAGCTACACATG
GGGCGTCTGTAACTACGCCGGTCATAGCGTTGGATACATTGGACGAGCCATCCGGAGATAACTGTGATCTCGATTCTCAA
CTATCAGACTGGAGTTCGTATTTGCAGTATACTTCGGGTTCAACGGCCAACCCCCGTGGTGTGGTTTTATCCATGCGTAA
CGTTACGGAAAATGTCGACCAAATTATCCGTAACTATTTTCGCCATGAGGGCGGCGCGCCGAGGTTGCCCAGCTCGGTCG
TTTCGTGGTTGCCGCTTTACCATGACATGGGTTTAATGGTTGGCCTCTTTATTCCGTTGTTTGTCGGATGTCCGGTTATC
CTGACGAGCCCAGAGGCATTTATCCGTAAGCCTGCCAGATGGATGCAACTGCTTGCTAAACACCAGGCGCCATTTTCGGC
CGCGCCGAACTTCGCATTCGATTTGGCCGTCGCTAAAACTTCCGAAGAGGACATGGCGGGGCTGGATTTAGGCCACGTAA
ATACAATAATCAACGGCGCGGAGCAGGTACAGCCAAATACAATAACCAAATTCCTCCGCCGGTTCCGTCCCTACAATTTG
ATGCCCGCAGCGGTCAAGCCATCATACGGGATGGCTGAAGCGGTGGTTTACCTGGCGACGACGAAGGCGGGATCACCTCC
AACGTCAACCGAGTTCGATGCTGATAGCTTGGCTCGAGGCCACGCGGAGCTAAGTACTTTCGAAACTGAGCGTGCAACGC
GTTTAATACGCTACCACAGCGACGACAAGGAACCGTTGCTTCGGATTGTCGATCCGGACTCGAATATCGAGCTCGGACCG
GGACGTATCGGCGAGATTTGGATTCACGGTAAGAATGTGTCTACCGGATATCACAATGCAGACGACGCGCTCAATCGAGA
TAAGTTCCAGGCCAGCATCCGGGAGGCCTCTGCGGGAACGCCAAGGTCGCCGTGGCTTCGCACGGGAGACTTGGGATTCA
TAGTAGGAGATGAGTTCTACATCGTCGGCCGTATGAAAGATCTCATTATCCAAGACGGTGTAAACCATTATCCCGATGAT
ATCGAAACTACGGTCAAGGAGTTTACCGGTGGCCGGGTCGCGGCATTTTCAGTATCCGACGACGGGGTGGAGCATTTGGT
CATTGCGGCCGAGGTAAGGACTGAGCATGGGCCCGATAAAGTGACTATTATGGATTTCTCGACGATCAAAAGGCTGGTCG
TATCGGCGTTGTCGAAATTACATGGCCTGCATGTAACAGATTTTCTTCTGGTACCGCCCGGGGCGCTACCGAAGACCACC
AGCGGAAAGATTAGCCGGGCGGCATGCGCAAAGCAGTACGGAGCAAATAAGTTGCAACGAGTAGCAACGTTCCCATGA

Upstream 100 bases:

>100_bases
AGGAGTGGAAACATGAGCGGCCCGACGGGAAATTCGATGCCCAGACAGCTCGGCGGCCTGGTGGCCAGGATCGTTACCGG
GTAAGGGATCGCCACTCCCA

Downstream 100 bases:

>100_bases
CAGACGGTTCGGTCACTGCGGATAAGCTTCAAAAATGGTTTCGAGAGTACTTGTCCACGCATATCGAGTGTCATCCAAAT
GAGGTCAGCCTAGACGTTCC

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MSVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHADRRRTAAISAPQGLDYVAGFL
GALCAGWTPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTSQAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQ
LSDWSSYLQYTSGSTANPRGVVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGAEQVQPNTITKFLRRFRPYNL
MPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARGHAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGP
GRIGEIWIHGKNVSTGYHNADDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKLHGLHVTDFLLVPPGALPKTT
SGKISRAACAKQYGANKLQRVATFP

Sequences:

>Translated_585_residues
MSVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHADRRRTAAISAPQGLDYVAGFL
GALCAGWTPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTSQAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQ
LSDWSSYLQYTSGSTANPRGVVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGAEQVQPNTITKFLRRFRPYNL
MPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARGHAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGP
GRIGEIWIHGKNVSTGYHNADDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKLHGLHVTDFLLVPPGALPKTT
SGKISRAACAKQYGANKLQRVATFP
>Mature_584_residues
SVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHADRRRTAAISAPQGLDYVAGFLG
ALCAGWTPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTSQAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQL
SDWSSYLQYTSGSTANPRGVVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVIL
TSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGAEQVQPNTITKFLRRFRPYNLM
PAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARGHAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGPG
RIGEIWIHGKNVSTGYHNADDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDDI
ETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKLHGLHVTDFLLVPPGALPKTTS
GKISRAACAKQYGANKLQRVATFP

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=504, Percent_Identity=25, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI225735629, Length=536, Percent_Identity=24.8134328358209, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI45827692, Length=536, Percent_Identity=24.8134328358209, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI55749758, Length=599, Percent_Identity=24.3739565943239, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI225735625, Length=612, Percent_Identity=22.8758169934641, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI45827694, Length=581, Percent_Identity=22.0309810671256, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI156151445, Length=421, Percent_Identity=23.5154394299287, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI45827696, Length=526, Percent_Identity=21.4828897338403, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI225735627, Length=526, Percent_Identity=21.4828897338403, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788107, Length=577, Percent_Identity=25.1299826689775, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI193202679, Length=628, Percent_Identity=22.1337579617834, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=550, Percent_Identity=23.2727272727273, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654656, Length=466, Percent_Identity=22.7467811158798, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 63844; Mature: 63713

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHAD
CCHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RRRTAAISAPQGLDYVAGFLGALCAGWTPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTS
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEECC
QAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQLSDWSSYLQYTSGSTANPRG
CHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCE
VVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGA
EECCHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHCCH
EQVQPNTITKFLRRFRPYNLMPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCCHHHHHHC
HAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGPGRIGEIWIHGKNVSTGYHNA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCCCCCC
DDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
HHHCCHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCEEEEEHHHHHHHHCCCCCCCCH
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKL
HHHHHHHHCCCEEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHH
HGLHVTDFLLVPPGALPKTTSGKISRAACAKQYGANKLQRVATFP
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure 
SVISTLRDRATTTPSDEAFVFMDYDTKTGDQIDRMTWSQLYSRVTAVSAYLISYGRHAD
CHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RRRTAAISAPQGLDYVAGFLGALCAGWTPVPLPEPLGSLRDKRTGLAVLDCAADVVLTTS
HHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEECC
QAETRVRATIATHGASVTTPVIALDTLDEPSGDNCDLDSQLSDWSSYLQYTSGSTANPRG
CHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCE
VVLSMRNVTENVDQIIRNYFRHEGGAPRLPSSVVSWLPLYHDMGLMVGLFIPLFVGCPVI
EEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTSPEAFIRKPARWMQLLAKHQAPFSAAPNFAFDLAVAKTSEEDMAGLDLGHVNTIINGA
EECCHHHHHCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHCCH
EQVQPNTITKFLRRFRPYNLMPAAVKPSYGMAEAVVYLATTKAGSPPTSTEFDADSLARG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCCHHHHHHC
HAELSTFETERATRLIRYHSDDKEPLLRIVDPDSNIELGPGRIGEIWIHGKNVSTGYHNA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCEEEEEEECCCCCCCCCCC
DDALNRDKFQASIREASAGTPRSPWLRTGDLGFIVGDEFYIVGRMKDLIIQDGVNHYPDD
HHHCCHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCEEEEEHHHHHHHHCCCCCCCCH
IETTVKEFTGGRVAAFSVSDDGVEHLVIAAEVRTEHGPDKVTIMDFSTIKRLVVSALSKL
HHHHHHHHCCCEEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHH
HGLHVTDFLLVPPGALPKTTSGKISRAACAKQYGANKLQRVATFP
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]