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The map label for this gene is purT [H]

Identifier: 148660154

GI number: 148660154

Start: 469645

End: 470904

Strand: Direct

Name: purT [H]

Synonym: MRA_0396

Alternate gene names: 148660154

Gene position: 469645-470904 (Clockwise)

Preceding gene: 148660152

Following gene: 148660155

Centisome position: 10.63

GC content: 67.7

Gene sequence:

>1260_bases
GTGATCGACGGCTGGACGGAAGAACAGCACGAACCCACCGTTAGGCATGAGCGCCCAGCAGCTCCCCAAGACGTTCGGCG
GGTGATGTTGCTGGGTTCGGCCGAACCCAGCCGGGAGCTGGCGATCGCGTTGCAGGGCTTGGGCGCGGAGGTGATCGCCG
TCGACGGCTATGTCGGCGCGCCTGCCCACCGGATAGCCGACCAGTCGGTGGTGGTCACCATGACCGATGCTGAAGAGCTG
ACGGCGGTGATCCGGCGGCTGCAACCGGATTTCTTGGTGACGGTCACCGCCGCGGTGTCTGTGGATGCTCTCGATGCCGT
CGAGCAAGCCGACGGCGAGTGCACTGAGCTGGTGCCGAACGCCCGTGCCGTCCGGTGCACGGCCGACCGGGAGGGCCTGC
GCCGGCTGGCCGCCGATCAGCTCGGCCTGCCCACAGCCCCGTTCTGGTTCGTCGGATCCCTTGGCGAACTTCAAGCGGTG
GCCGTCCATGCTGGGTTTCCGTTGCTGGTGAGCCCGGTGGCAGGGGTGGCTGGCCAGGGTAGCTCGGTGGTCGCCGGGCC
CAACGAGGTCGAGCCCGCCTGGCAGCGCGCGGCAGGCCATCAAGTACAGCCGCAGACTGGGGGAGTGAGCCCTCGGGTGT
GCGCCGAGTCGGTGGTCGAGATCGAGTTTTTGGTCACCATGATCGTTGTGTGCAGTCAGGGCCCGAACGGGCCGCTCATC
GAGTTCTGTGCACCTATCGGTCATCGCGACGCCGATGCCGGTGAGTTGGAATCCTGGCAACCGCAGAAGCTGAGCACGGC
GGCGCTGGACGCGGCCAAGTCGATCGCCGCGCGCATCGTCAAGGCGCTCGGGGGACGCGGGGTTTTCGGCGTCGAATTGA
TGATCAACGGCGATGAGGTGTATTTCGCCGATGTCACCGTGTGTCCTGCCGGGAGTGCCTGGGTCACCGTGCGCAGCCAG
CGGCTTTCGGTGTTCGAACTGCAGGCCCGGGCGATCCTGGGTCTGGCGGTGGACACCCTGATGATCTCGCCGGGTGCCGC
GCGGGTGATCAACCCGGACCACACGGCAGGCCGGGCAGCGGTCGGCGCCGCACCACCTGCCGATGCGCTGACCGGTGCGC
TCGGTGTGCCGGAAAGCGACGTCGTGATATTCGGCCGCGGGCTTGGGGTGGCGCTGGCCACCGCACCCGAGGTGGCAATC
GCCCGCGAACGCGCCCGCGAAGTTGCATCTCGGCTAAATGTGCCAGACTCACGCGAGTGA

Upstream 100 bases:

>100_bases
CCCACCTCCTCATAGCAAAAGTACACAGGCAGATGTGAGCGGAGTTGCGAAAATAGACAAATAATTGAGCCGAGCAACGA
CCGAGCGAGAGGGTGAGCTG

Downstream 100 bases:

>100_bases
GCTACGCCGGAGATATCACGCCACTTCAGGCCTGGGAGATGCTCAGCGATAATCCGCGGGCGGTCCTGGTCGACGTGCGC
TGCGAGGCGGAATGGCGCTT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MIDGWTEEQHEPTVRHERPAAPQDVRRVMLLGSAEPSRELAIALQGLGAEVIAVDGYVGAPAHRIADQSVVVTMTDAEEL
TAVIRRLQPDFLVTVTAAVSVDALDAVEQADGECTELVPNARAVRCTADREGLRRLAADQLGLPTAPFWFVGSLGELQAV
AVHAGFPLLVSPVAGVAGQGSSVVAGPNEVEPAWQRAAGHQVQPQTGGVSPRVCAESVVEIEFLVTMIVVCSQGPNGPLI
EFCAPIGHRDADAGELESWQPQKLSTAALDAAKSIAARIVKALGGRGVFGVELMINGDEVYFADVTVCPAGSAWVTVRSQ
RLSVFELQARAILGLAVDTLMISPGAARVINPDHTAGRAAVGAAPPADALTGALGVPESDVVIFGRGLGVALATAPEVAI
ARERAREVASRLNVPDSRE

Sequences:

>Translated_419_residues
MIDGWTEEQHEPTVRHERPAAPQDVRRVMLLGSAEPSRELAIALQGLGAEVIAVDGYVGAPAHRIADQSVVVTMTDAEEL
TAVIRRLQPDFLVTVTAAVSVDALDAVEQADGECTELVPNARAVRCTADREGLRRLAADQLGLPTAPFWFVGSLGELQAV
AVHAGFPLLVSPVAGVAGQGSSVVAGPNEVEPAWQRAAGHQVQPQTGGVSPRVCAESVVEIEFLVTMIVVCSQGPNGPLI
EFCAPIGHRDADAGELESWQPQKLSTAALDAAKSIAARIVKALGGRGVFGVELMINGDEVYFADVTVCPAGSAWVTVRSQ
RLSVFELQARAILGLAVDTLMISPGAARVINPDHTAGRAAVGAAPPADALTGALGVPESDVVIFGRGLGVALATAPEVAI
ARERAREVASRLNVPDSRE
>Mature_419_residues
MIDGWTEEQHEPTVRHERPAAPQDVRRVMLLGSAEPSRELAIALQGLGAEVIAVDGYVGAPAHRIADQSVVVTMTDAEEL
TAVIRRLQPDFLVTVTAAVSVDALDAVEQADGECTELVPNARAVRCTADREGLRRLAADQLGLPTAPFWFVGSLGELQAV
AVHAGFPLLVSPVAGVAGQGSSVVAGPNEVEPAWQRAAGHQVQPQTGGVSPRVCAESVVEIEFLVTMIVVCSQGPNGPLI
EFCAPIGHRDADAGELESWQPQKLSTAALDAAKSIAARIVKALGGRGVFGVELMINGDEVYFADVTVCPAGSAWVTVRSQ
RLSVFELQARAILGLAVDTLMISPGAARVINPDHTAGRAAVGAAPPADALTGALGVPESDVVIFGRGLGVALATAPEVAI
ARERAREVASRLNVPDSRE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=403, Percent_Identity=41.1910669975186, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43678; Mature: 43678

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGWTEEQHEPTVRHERPAAPQDVRRVMLLGSAEPSRELAIALQGLGAEVIAVDGYVGA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHEEEEEECCCEEEEEECCCCCC
PAHRIADQSVVVTMTDAEELTAVIRRLQPDFLVTVTAAVSVDALDAVEQADGECTELVPN
CHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCHHHHCCC
ARAVRCTADREGLRRLAADQLGLPTAPFWFVGSLGELQAVAVHAGFPLLVSPVAGVAGQG
CCEEEEECCHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCC
SSVVAGPNEVEPAWQRAAGHQVQPQTGGVSPRVCAESVVEIEFLVTMIVVCSQGPNGPLI
CEEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EFCAPIGHRDADAGELESWQPQKLSTAALDAAKSIAARIVKALGGRGVFGVELMINGDEV
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEE
YFADVTVCPAGSAWVTVRSQRLSVFELQARAILGLAVDTLMISPGAARVINPDHTAGRAA
EEEEEEECCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHEEECCCCCEEECCCCCCCCEE
VGAAPPADALTGALGVPESDVVIFGRGLGVALATAPEVAIARERAREVASRLNVPDSRE
CCCCCCHHHHHCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDGWTEEQHEPTVRHERPAAPQDVRRVMLLGSAEPSRELAIALQGLGAEVIAVDGYVGA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHEEEEEECCCEEEEEECCCCCC
PAHRIADQSVVVTMTDAEELTAVIRRLQPDFLVTVTAAVSVDALDAVEQADGECTELVPN
CHHHHCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCHHHHCCC
ARAVRCTADREGLRRLAADQLGLPTAPFWFVGSLGELQAVAVHAGFPLLVSPVAGVAGQG
CCEEEEECCHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCC
SSVVAGPNEVEPAWQRAAGHQVQPQTGGVSPRVCAESVVEIEFLVTMIVVCSQGPNGPLI
CEEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EFCAPIGHRDADAGELESWQPQKLSTAALDAAKSIAARIVKALGGRGVFGVELMINGDEV
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEE
YFADVTVCPAGSAWVTVRSQRLSVFELQARAILGLAVDTLMISPGAARVINPDHTAGRAA
EEEEEEECCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHEEECCCCCEEECCCCCCCCEE
VGAAPPADALTGALGVPESDVVIFGRGLGVALATAPEVAIARERAREVASRLNVPDSRE
CCCCCCHHHHHCCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA