Click here to switch to the map view.

The map label for this gene is fba

Identifier: 148660129

GI number: 148660129

Start: 442628

End: 443662

Strand: Reverse

Name: fba

Synonym: MRA_0372

Alternate gene names: 148660129

Gene position: 443662-442628 (Counterclockwise)

Preceding gene: 148660131

Following gene: 148660126

Centisome position: 10.04

GC content: 62.51

Gene sequence:

>1035_bases
ATGCCTATCGCAACGCCCGAGGTCTACGCGGAGATGCTCGGTCAGGCCAAACAAAACTCGTACGCTTTCCCGGCTATCAA
CTGCACCTCCTCGGAAACCGTCAACGCCGCGATCAAAGGTTTCGCCGACGCCGGCAGTGACGGAATCATCCAGTTCTCGA
CCGGTGGCGCAGAATTCGGCTCCGGCCTCGGGGTCAAAGACATGGTGACCGGTGCGGTCGCCTTGGCGGAGTTCACCCAC
GTTATCGCGGCCAAGTACCCGGTCAACGTGGCGCTGCACACCGACCACTGCCCCAAGGACAAGTTGGACAGCTATGTCCG
GCCCTTGCTGGCGATCTCGGCGCAACGCGTGAGCAAAGGTGGCAATCCTTTGTTCCAGTCGCACATGTGGGACGGCTCGG
CAGTGCCAATCGATGAGAACCTGGCCATCGCCCAGGAGCTGCTCAAGGCGGCGGCGGCCGCCAAGATCATTCTGGAGATC
GAGATCGGCGTCGTCGGCGGCGAAGAGGACGGCGTGGCGAACGAGATCAACGAGAAGCTGTACACCAGCCCGGAGGACTT
CGAGAAAACCATCGAGGCGCTGGGCGCCGGTGAGCACGGCAAATACCTGCTGGCCGCGACGTTCGGCAACGTGCATGGCG
TCTACAAGCCCGGCAACGTCAAGCTTCGCCCCGACATCCTTGCGCAAGGGCAACAGGTGGCGGCGGCCAAGCTCGGACTG
CCGGCCGACGCCAAGCCGTTCGACTTCGTGTTCCACGGCGGCTCGGGTTCGCTTAAGTCGGAGATCGAGGAGGCGCTGCG
CTACGGCGTGGTGAAGATGAACGTCGACACCGACACCCAGTACGCGTTCACCCGCCCGATCGCCGGTCACATGTTCACCA
ACTACGACGGAGTGCTCAAGGTCGATGGCGAGGTGGGTGTCAAGAAGGTCTACGACCCGCGCAGCTACCTCAAGAAGGCC
GAAGCTTCGATGAGCCAGCGGGTCGTTCAGGCGTGCAATGACCTGCACTGCGCCGGAAAGTCCCTAACCCACTAA

Upstream 100 bases:

>100_bases
CTCATGACGTCCCAACATACCGGCCATCACGCACAGGCCGACAAGCTCGGTTGCGGTGTTGCGATACTGGGTGGATCGGA
GTCGTCAGGAGGAACGTTTC

Downstream 100 bases:

>100_bases
ACCGGGGCCGCTCACAAGCCGTGCAGCTCGGTCAGCGTCAGGTGCGCGACCAGGAAGTAGATGAGCAGACCCGTGCCGTC
AACGATGGTGGCGATCATCG

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MPIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGVKDMVTGAVALAEFTH
VIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEI
EIGVVGGEEDGVANEINEKLYTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGL
PADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYLKKA
EASMSQRVVQACNDLHCAGKSLTH

Sequences:

>Translated_344_residues
MPIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGVKDMVTGAVALAEFTH
VIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEI
EIGVVGGEEDGVANEINEKLYTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGL
PADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYLKKA
EASMSQRVVQACNDLHCAGKSLTH
>Mature_343_residues
PIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGVKDMVTGAVALAEFTHV
IAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEIE
IGVVGGEEDGVANEINEKLYTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGLP
ADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYLKKAE
ASMSQRVVQACNDLHCAGKSLTH

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI1789293, Length=337, Percent_Identity=40.9495548961424, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082041, Length=331, Percent_Identity=25.3776435045317, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789526, Length=325, Percent_Identity=24.6153846153846, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322790, Length=332, Percent_Identity=37.9518072289157, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF_MYCBO (P67476)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854033.1
- ProteinModelPortal:   P67476
- SMR:   P67476
- EnsemblBacteria:   EBMYCT00000014332
- GeneID:   1091279
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0370c
- GeneTree:   EBGT00050000016874
- HOGENOM:   HBG327581
- OMA:   QAYCAEK
- ProtClustDB:   PRK09197
- BioCyc:   MBOV233413:MB0370C-MONOMER
- BRENDA:   4.1.2.13
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR006411
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01520

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 36545; Mature: 36414

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 95-95 BINDING 53-53 BINDING 213-213

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFG
CCCCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHC
SGLGVKDMVTGAVALAEFTHVIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKG
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEIEIGVVGGEEDGVANEINEKL
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCHHHHHHHH
YTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGL
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEECCCCEEECHHHHHCCCHHHHHHCCC
PADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLK
CCCCCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCEEEECCCCCCCCCCCCCEEE
VDGEVGVKKVYDPRSYLKKAEASMSQRVVQACNDLHCAGKSLTH
ECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFG
CCCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHC
SGLGVKDMVTGAVALAEFTHVIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKG
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GNPLFQSHMWDGSAVPIDENLAIAQELLKAAAAAKIILEIEIGVVGGEEDGVANEINEKL
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCHHHHHHHH
YTSPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGL
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEECCCCEEECHHHHHCCCHHHHHHCCC
PADAKPFDFVFHGGSGSLKSEIEEALRYGVVKMNVDTDTQYAFTRPIAGHMFTNYDGVLK
CCCCCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCEEEECCCCCCCCCCCCCEEE
VDGEVGVKKVYDPRSYLKKAEASMSQRVVQACNDLHCAGKSLTH
ECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972