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The map label for this gene is narK3 [H]

Identifier: 148660026

GI number: 148660026

Start: 314121

End: 315530

Strand: Reverse

Name: narK3 [H]

Synonym: MRA_0269

Alternate gene names: 148660026

Gene position: 315530-314121 (Counterclockwise)

Preceding gene: 148660027

Following gene: 148660025

Centisome position: 7.14

GC content: 63.9

Gene sequence:

>1410_bases
ATGGGCCGTTCCCACCAGATCTCCGACTGGGATCCCGAAGACTCGGTGGCGTGGGAGGCCGGCAACAAATTCATCGCCCG
ACGCAATCTGATCTGGTCGGTGGCCGCCGAGCACGTCGGATTTTCCGTCTGGTCCCTCTGGTCGGTGATGGTGCTGTTCA
TGCCCACATCGGTGTACGGCTTTTCCGCCGGCGACAAGTTCCTGCTCGGCGCCACCGCGACCTTGGTCGGGGCATGCCTG
CGCTTCCCTTACACATTTGCCACCGCAAAGTTCGGGGGGCGCAACTGGACCATCTTTTCCGCACTGGTGCTGTTGATTCC
GACCGTTGGCAGCATTCTGTTGCTGGCCAACCCTGGTTTGCCACTGTGGCCGTATCTGGTGTGCGGTGCGCTAGCCGGCC
TCGGCGGCGGCAACTTCGCCGCCTCCATGACGAACATCAACGCTTTCTTTCCGCAACGACTCAAGGGCGCCGCGCTTGCG
CTCAACGCGGGTGGCGGCAACCTCGGGGTGCCGATGGTGCAGTTGGTCGGCCTGCTGGTGATCGCAACGGCCGGTGACCG
CGAGCCCTACTGGGTGTGCGCGATATATCTGGTGCTGCTGGCGGTCGCCGGCCTCGGCGCCGCACTGTACATGGACAACC
TGACGGAGTATCGCATCGAGCTGAACACCATGCGTGCGGTAGTGTCCGAGCCGCACACCTGGGTGATCTCGCTGCTGTAC
ATCGGCACTTTCGGCTCGTTCATCGGGTTCTCTTTCGCATTCGGTCAGGTGCTGCAGATCAACTTCATCGCCAGCGGTCA
GAGCACGGCGCAGGCCTCGCTGCATGCGGCCCAGATCGCCTTCCTGGGGCCGCTATTGGGATCGCTGTCCCGAATATATG
GCGGCAAGCTCGCCGACCGTATCGGTGGCGGCCGAGTCACCCTAGCTGCCTTCTGCGCCATGCTGCTGGCCACCGGAATA
CTCATCAGCGCAAGCACTTTCGGTGACCACCTGGCCGGGCCGATGCCGACTGCGACGATGGTCGGCTACGTCATCGGCTT
CACGGCTCTGTTCATTTTGTCCGGCATCGGCAATGGCTCTGTGTACAAGATGATTCCGTCGATCTTCGAGGCGCGCAGCC
ATTCGCTGCAGATCAGTGAAGCCGAGCGCAGGCAGTGGTCGCGGTCGATGTCAGGCGCGCTGATCGGCCTGGCCGGGGCG
GTCGGCGCGCTCGGCGGAGTGGGCGTCAACCTGGCTCTGCGAGAGTCCTACCTGACCAGTGGCACGGCCACCTCGGCATT
TTGGGCCTTCGGGGTGTTCTACCTGGTCGCCTCGGTGCTGACCTGGGCGATTTATGTGCGTCGGGGCCTAAAGAGCGCCG
GCGAGCTCGTGCCGGCGACCACCGCACCCGCGGGCTTGGCCTACGTCTAG

Upstream 100 bases:

>100_bases
CCGTGTGAGCGAAAGTTCACGCCGGGGTCACTACCGGAAGCGCCCGGGCAACAAAGGATTTCTAGCGTGATCGAGAACGT
CGATCACGATAGGGGTCAGT

Downstream 100 bases:

>100_bases
CGCCACGCCCGAAGCCACAGAGGTGGGTATCGGCAATGGGCAATCCGGCAGCAATTGCCTGGTAACGCGACTGAAACCTC
ACAGGCCTAGACACGTCATA

Product: integral membrane nitrite extrusion protein NarK3

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MGRSHQISDWDPEDSVAWEAGNKFIARRNLIWSVAAEHVGFSVWSLWSVMVLFMPTSVYGFSAGDKFLLGATATLVGACL
RFPYTFATAKFGGRNWTIFSALVLLIPTVGSILLLANPGLPLWPYLVCGALAGLGGGNFAASMTNINAFFPQRLKGAALA
LNAGGGNLGVPMVQLVGLLVIATAGDREPYWVCAIYLVLLAVAGLGAALYMDNLTEYRIELNTMRAVVSEPHTWVISLLY
IGTFGSFIGFSFAFGQVLQINFIASGQSTAQASLHAAQIAFLGPLLGSLSRIYGGKLADRIGGGRVTLAAFCAMLLATGI
LISASTFGDHLAGPMPTATMVGYVIGFTALFILSGIGNGSVYKMIPSIFEARSHSLQISEAERRQWSRSMSGALIGLAGA
VGALGGVGVNLALRESYLTSGTATSAFWAFGVFYLVASVLTWAIYVRRGLKSAGELVPATTAPAGLAYV

Sequences:

>Translated_469_residues
MGRSHQISDWDPEDSVAWEAGNKFIARRNLIWSVAAEHVGFSVWSLWSVMVLFMPTSVYGFSAGDKFLLGATATLVGACL
RFPYTFATAKFGGRNWTIFSALVLLIPTVGSILLLANPGLPLWPYLVCGALAGLGGGNFAASMTNINAFFPQRLKGAALA
LNAGGGNLGVPMVQLVGLLVIATAGDREPYWVCAIYLVLLAVAGLGAALYMDNLTEYRIELNTMRAVVSEPHTWVISLLY
IGTFGSFIGFSFAFGQVLQINFIASGQSTAQASLHAAQIAFLGPLLGSLSRIYGGKLADRIGGGRVTLAAFCAMLLATGI
LISASTFGDHLAGPMPTATMVGYVIGFTALFILSGIGNGSVYKMIPSIFEARSHSLQISEAERRQWSRSMSGALIGLAGA
VGALGGVGVNLALRESYLTSGTATSAFWAFGVFYLVASVLTWAIYVRRGLKSAGELVPATTAPAGLAYV
>Mature_468_residues
GRSHQISDWDPEDSVAWEAGNKFIARRNLIWSVAAEHVGFSVWSLWSVMVLFMPTSVYGFSAGDKFLLGATATLVGACLR
FPYTFATAKFGGRNWTIFSALVLLIPTVGSILLLANPGLPLWPYLVCGALAGLGGGNFAASMTNINAFFPQRLKGAALAL
NAGGGNLGVPMVQLVGLLVIATAGDREPYWVCAIYLVLLAVAGLGAALYMDNLTEYRIELNTMRAVVSEPHTWVISLLYI
GTFGSFIGFSFAFGQVLQINFIASGQSTAQASLHAAQIAFLGPLLGSLSRIYGGKLADRIGGGRVTLAAFCAMLLATGIL
ISASTFGDHLAGPMPTATMVGYVIGFTALFILSGIGNGSVYKMIPSIFEARSHSLQISEAERRQWSRSMSGALIGLAGAV
GALGGVGVNLALRESYLTSGTATSAFWAFGVFYLVASVLTWAIYVRRGLKSAGELVPATTAPAGLAYV

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=470, Percent_Identity=34.6808510638298, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1787743, Length=467, Percent_Identity=33.8329764453961, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49357; Mature: 49226

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRSHQISDWDPEDSVAWEAGNKFIARRNLIWSVAAEHVGFSVWSLWSVMVLFMPTSVYG
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHEE
FSAGDKFLLGATATLVGACLRFPYTFATAKFGGRNWTIFSALVLLIPTVGSILLLANPGL
CCCCCCEEEHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEECCCC
PLWPYLVCGALAGLGGGNFAASMTNINAFFPQRLKGAALALNAGGGNLGVPMVQLVGLLV
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHH
IATAGDREPYWVCAIYLVLLAVAGLGAALYMDNLTEYRIELNTMRAVVSEPHTWVISLLY
EEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGTFGSFIGFSFAFGQVLQINFIASGQSTAQASLHAAQIAFLGPLLGSLSRIYGGKLADR
HHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGGGRVTLAAFCAMLLATGILISASTFGDHLAGPMPTATMVGYVIGFTALFILSGIGNGS
CCCCEEHHHHHHHHHHHHHHHHEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
VYKMIPSIFEARSHSLQISEAERRQWSRSMSGALIGLAGAVGALGGVGVNLALRESYLTS
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEHEEHHHHHCC
GTATSAFWAFGVFYLVASVLTWAIYVRRGLKSAGELVPATTAPAGLAYV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRSHQISDWDPEDSVAWEAGNKFIARRNLIWSVAAEHVGFSVWSLWSVMVLFMPTSVYG
CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHEE
FSAGDKFLLGATATLVGACLRFPYTFATAKFGGRNWTIFSALVLLIPTVGSILLLANPGL
CCCCCCEEEHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEECCCC
PLWPYLVCGALAGLGGGNFAASMTNINAFFPQRLKGAALALNAGGGNLGVPMVQLVGLLV
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHH
IATAGDREPYWVCAIYLVLLAVAGLGAALYMDNLTEYRIELNTMRAVVSEPHTWVISLLY
EEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGTFGSFIGFSFAFGQVLQINFIASGQSTAQASLHAAQIAFLGPLLGSLSRIYGGKLADR
HHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGGGRVTLAAFCAMLLATGILISASTFGDHLAGPMPTATMVGYVIGFTALFILSGIGNGS
CCCCEEHHHHHHHHHHHHHHHHEECCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
VYKMIPSIFEARSHSLQISEAERRQWSRSMSGALIGLAGAVGALGGVGVNLALRESYLTS
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEHEEHHHHHCC
GTATSAFWAFGVFYLVASVLTWAIYVRRGLKSAGELVPATTAPAGLAYV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]