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The map label for this gene is ilvD [H]

Identifier: 148659953

GI number: 148659953

Start: 221356

End: 223083

Strand: Reverse

Name: ilvD [H]

Synonym: MRA_0197

Alternate gene names: 148659953

Gene position: 223083-221356 (Counterclockwise)

Preceding gene: 148659957

Following gene: 148659946

Centisome position: 5.05

GC content: 65.86

Gene sequence:

>1728_bases
ATGCCGCAAACCACCGACGAAGCCGCTTCCGTCTCGACGGTCGCCGACATCAAGCCCCGCAGTCGAGACGTCACCGACGG
CCTGGAGAAGGCCGCTGCCCGGGGCATGTTGAGGGCGGTGGGCATGGACGACGAAGACTTCGCGAAGCCACAGATCGGGG
TCGCGTCGTCGTGGAACGAAATCACGCCGTGCAATCTCTCATTGGACCGGCTGGCCAACGCGGTCAAGGAGGGGGTGTTT
TCGGCCGGCGGCTATCCGCTTGAGTTCGGCACGATCTCGGTTTCGGACGGCATCTCCATGGGTCATGAGGGGATGCACTT
CTCGCTGGTTTCCCGTGAGGTGATCGCCGACAGCGTCGAAGTCGTGATGCAGGCCGAACGACTCGACGGTTCGGTGCTGC
TGGCCGGATGCGACAAATCGCTGCCCGGAATGCTGATGGCCGCCGCACGACTGGATCTGGCGGCGGTGTTCCTCTACGCG
GGCTCTATTTTGCCCGGCCGGGCCAAGCTCTCCGACGGTAGCGAACGAGACGTCACCATCATTGACGCCTTCGAGGCGGT
CGGAGCGTGCTCTCGCGGTTTGATGAGTCGCGCCGACGTCGATGCCATCGAGCGGGCAATCTGCCCCGGCGAAGGGGCAT
GCGGCGGCATGTACACCGCTAACACCATGGCCAGCGCCGCCGAGGCGCTCGGCATGTCGTTGCCGGGCAGCGCGGCACCG
CCGGCGACCGACCGCCGGCGCGACGGGTTCGCGCGTCGCAGCGGGCAGGCCGTCGTCGAGCTGCTGCGCCGCGGCATCAC
CGCCCGCGACATCCTCACCAAGGAGGCGTTCGAGAACGCCATCGCGGTGGGAATGGCGTTCGGCGGCTCGACCAACGCGG
TGCTGCATCTGCTGGCCATCGCCCACGAGGCCAACGTCGCACTATCGCTTCAGGACTTCAGCCGGATCGGGTCGGGGGTG
CCGCATTTGGCCGATGTCAAGCCGTTCGGCCGCCATGTGATGTCCGACGTCGATCACATCGGCGGTGTGCCGGTGGTTAT
GAAGGCGCTGCTGGACGCCGGTCTGCTACACGGCGACTGCCTGACGGTTACCGGTCACACCATGGCCGAGAATCTGGCTG
CCATCACCCCGCCGGATCCAGATGGCAAGGTGCTACGTGCGTTGGCAAACCCGATCCACCCGAGCGGTGGCATCACCATC
CTGCACGGATCGCTGGCACCCGAAGGTGCGGTGGTCAAGACCGCCGGATTCGATTCCGACGTATTCGAAGGCACCGCAAG
AGTTTTCGACGGCGAGCGCGCTGCGCTGGACGCCCTTGAGGATGGCACCATTACCGTGGGCGACGCCGTGGTAATCCGGT
ACGAAGGACCCAAGGGCGGCCCCGGGATGCGCGAAATGCTCGCCATCACAGGCGCGATCAAAGGCGCAGGACTCGGTAAA
GATGTTCTACTGCTGACCGACGGCCGGTTCTCCGGTGGAACCACCGGCCTGTGCGTTGGCCACATCGCACCGGAGGCGGT
CGACGGCGGACCGATCGCACTTCTGCGCAACGGCGACCGGATCCGGCTCGACGTGGCCGGCCGCGTTCTCGATGTGTTGG
CCGATCCGGCCGAATTCGCGTCCCGACAACAGGATTTCAGTCCTCCACCGCCGCGCTACACTACTGGCGTACTGTCCAAG
TACGTCAAGCTGGTCAGCTCTGCCGCGGTCGGCGCGGTCTGCGGCTAG

Upstream 100 bases:

>100_bases
GGCATGCTACCCATCATATCTTGTACCCCAGCGGGGTATCGGACCTAGAATTTGGCAACCGTGCCGCGGCGAGCGCATCC
TTGGGCCGCATACTTGAACA

Downstream 100 bases:

>100_bases
CCGCAACTAGCCGCTGCCTGGCCGGGACCGCCCCGGCCAGGACATTCCCGAGTTGCCCTCTGGCTCGCGAACTTCGAACT
ATCCTTGGTTAGCCGCGGCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MPQTTDEAASVSTVADIKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKEGVF
SAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYA
GSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAP
PATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVGMAFGGSTNAVLHLLAIAHEANVALSLQDFSRIGSGV
PHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAITPPDPDGKVLRALANPIHPSGGITI
LHGSLAPEGAVVKTAGFDSDVFEGTARVFDGERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGK
DVLLLTDGRFSGGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPRYTTGVLSK
YVKLVSSAAVGAVCG

Sequences:

>Translated_575_residues
MPQTTDEAASVSTVADIKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKEGVF
SAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYA
GSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAP
PATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVGMAFGGSTNAVLHLLAIAHEANVALSLQDFSRIGSGV
PHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAITPPDPDGKVLRALANPIHPSGGITI
LHGSLAPEGAVVKTAGFDSDVFEGTARVFDGERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGK
DVLLLTDGRFSGGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPRYTTGVLSK
YVKLVSSAAVGAVCG
>Mature_574_residues
PQTTDEAASVSTVADIKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKEGVFS
AGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYAG
SILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAPP
ATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVGMAFGGSTNAVLHLLAIAHEANVALSLQDFSRIGSGVP
HLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAITPPDPDGKVLRALANPIHPSGGITIL
HGSLAPEGAVVKTAGFDSDVFEGTARVFDGERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGKD
VLLLTDGRFSGGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPRYTTGVLSKY
VKLVSSAAVGAVCG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=39.344262295082, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI2367371, Length=528, Percent_Identity=34.0909090909091, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786464, Length=486, Percent_Identity=36.4197530864198, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1788157, Length=581, Percent_Identity=30.1204819277108, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6322476, Length=576, Percent_Identity=47.0486111111111, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59310; Mature: 59179

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00626 RCC1_2 ; PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTTDEAASVSTVADIKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNE
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
ITPCNLSLDRLANAVKEGVFSAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVE
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHH
VVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTI
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
IDAFEAVGACSRGLMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAP
EEHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
PATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVGMAFGGSTNAVLHLLAI
CCCCCHHCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
AHEANVALSLQDFSRIGSGVPHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDC
HHHCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCE
LTVTGHTMAENLAAITPPDPDGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGFDSD
EEECCCHHHHCCEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCH
VFEGTARVFDGERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGK
HHHHHHHHCCCCHHHHHHHCCCCEEECCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
DVLLLTDGRFSGGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFA
EEEEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHCCCHHHH
SRQQDFSPPPPRYTTGVLSKYVKLVSSAAVGAVCG
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQTTDEAASVSTVADIKPRSRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNE
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCC
ITPCNLSLDRLANAVKEGVFSAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVE
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHH
VVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTI
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
IDAFEAVGACSRGLMSRADVDAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAP
EEHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
PATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVGMAFGGSTNAVLHLLAI
CCCCCHHCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
AHEANVALSLQDFSRIGSGVPHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDC
HHHCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCE
LTVTGHTMAENLAAITPPDPDGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGFDSD
EEECCCHHHHCCEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCH
VFEGTARVFDGERAALDALEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGK
HHHHHHHHCCCCHHHHHHHCCCCEEECCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
DVLLLTDGRFSGGTTGLCVGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFA
EEEEEECCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHCCCHHHH
SRQQDFSPPPPRYTTGVLSKYVKLVSSAAVGAVCG
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA