BacMap

Definition	Mycobacterium tuberculosis H37Ra, complete genome.
Accession	NC_009525
Length	4,419,977

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The map label for this gene is fabG [C]

Identifier: 148659912

GI number: 148659912

Start: 176192

End: 177052

Strand: Direct

Name: fabG [C]

Synonym: MRA_0155

Alternate gene names: 148659912

Gene position: 176192-177052 (Clockwise)

Preceding gene: 148659911

Following gene: 148659913

Centisome position: 3.99

GC content: 64.23

Gene sequence:

>861_bases
ATGCCCGGAGTGCAAGATCGCGTCATCGTCGTTACTGGAGCCGGCGGTGGCTTGGGCCGCGAATACGCCCTTACGCTCGC
CGGGGAGGGCGCCAGCGTCGTGGTCAACGACCTCGGTGGCGCCCGCGACGGCACGGGCGCCGGTTCGGCGATGGCCGATG
AGGTCGTCGCCGAGATTCGCGACAAGGGGGGCCGGGCGGTCGCCAACTACGACAGCGTCGCCACCGAGGACGGCGCAGCG
AACATCATCAAGACCGCGCTTGACGAATTCGGCGCCGTGCACGGTGTGGTGAGCAACGCCGGGATCTTGCGCGACGGCAC
CTTCCACAAGATGTCGTTCGAGAATTGGGACGCCGTGCTTAAGGTGCACCTTTATGGCGGATACCACGTGCTACGCGCGG
CCTGGCCGCATTTCCGTGAGCAGAGTTACGGCCGGGTCGTGGTGGCGACCTCCACCAGCGGGCTGTTCGGCAACTTCGGC
CAGACCAACTATGGGGCGGCCAAGCTTGGTCTGGTCGGCCTGATCAATACGCTGGCGCTGGAGGGAGCCAAGTACAACAT
CCACGCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCAGGACATCCTGCCGCCCGAAGTACTGGAAAAGCTCA
CACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCGAGGAGTGTGCCGACAACGCATCGGTGTACGTCGTCGGT
GGTGGCAAGGTGCAGCGAGTTGCGCTGTTTGGCAACGACGGCGCCAACTTCGACAAACCGCCGTCGGTACAAGATGTTGC
GGCGCGGTGGGCCGAGATCACCGATCTGTCCGGTGCGAAAATTGCTGGATTCAAGTTGTAG

Upstream 100 bases:

>100_bases
TCAAGGTGGCTCGCCGGCTGTTCTGACCTGGGCGCGGGTTGTCGCCCCGTTGACACCCGACTCGTTATAACCCCGAATTG
TGATTGCGGAGAGGAGCCTG

Downstream 100 bases:

>100_bases
AAGTAAATGAAGGCTTGTGTCGTAAAAGAACTTTCCGGCCCGTCCGGCATGGTGTACACCGACATCGACGAGGTATCCGG
TGACGGCGGAAAGGTTGTTA

Product: putative short-chain type dehydrogenase/reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MPGVQDRVIVVTGAGGGLGREYALTLAGEGASVVVNDLGGARDGTGAGSAMADEVVAEIRDKGGRAVANYDSVATEDGAA
NIIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVLKVHLYGGYHVLRAAWPHFREQSYGRVVVATSTSGLFGNFG
QTNYGAAKLGLVGLINTLALEGAKYNIHANALAPIAATRMTQDILPPEVLEKLTPEFVAPVVAYLCTEECADNASVYVVG
GGKVQRVALFGNDGANFDKPPSVQDVAARWAEITDLSGAKIAGFKL

Sequences:

>Translated_286_residues
MPGVQDRVIVVTGAGGGLGREYALTLAGEGASVVVNDLGGARDGTGAGSAMADEVVAEIRDKGGRAVANYDSVATEDGAA
NIIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVLKVHLYGGYHVLRAAWPHFREQSYGRVVVATSTSGLFGNFG
QTNYGAAKLGLVGLINTLALEGAKYNIHANALAPIAATRMTQDILPPEVLEKLTPEFVAPVVAYLCTEECADNASVYVVG
GGKVQRVALFGNDGANFDKPPSVQDVAARWAEITDLSGAKIAGFKL
>Mature_285_residues
PGVQDRVIVVTGAGGGLGREYALTLAGEGASVVVNDLGGARDGTGAGSAMADEVVAEIRDKGGRAVANYDSVATEDGAAN
IIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVLKVHLYGGYHVLRAAWPHFREQSYGRVVVATSTSGLFGNFGQ
TNYGAAKLGLVGLINTLALEGAKYNIHANALAPIAATRMTQDILPPEVLEKLTPEFVAPVVAYLCTEECADNASVYVVGG
GKVQRVALFGNDGANFDKPPSVQDVAARWAEITDLSGAKIAGFKL

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI4504505, Length=276, Percent_Identity=52.1739130434783, Blast_Score=286, Evalue=1e-77,
Organism=Homo sapiens, GI15277342, Length=254, Percent_Identity=35.0393700787402, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI40254992, Length=213, Percent_Identity=33.8028169014084, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4503817, Length=194, Percent_Identity=26.8041237113402, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=27.34375, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI19923817, Length=196, Percent_Identity=29.5918367346939, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787335, Length=237, Percent_Identity=31.2236286919831, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI87082100, Length=186, Percent_Identity=30.1075268817204, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789208, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790717, Length=212, Percent_Identity=27.3584905660377, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787526, Length=231, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789057, Length=206, Percent_Identity=31.5533980582524, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788459, Length=237, Percent_Identity=29.535864978903, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082160, Length=192, Percent_Identity=26.0416666666667, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786812, Length=189, Percent_Identity=28.042328042328, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=277, Percent_Identity=48.7364620938628, Blast_Score=274, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=34.6613545816733, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=28.515625, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=29.6, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=241, Percent_Identity=27.8008298755187, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567345, Length=179, Percent_Identity=29.0502793296089, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=25.6603773584906, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=187, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=194, Percent_Identity=24.7422680412371, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=281, Percent_Identity=50.5338078291815, Blast_Score=270, Evalue=2e-73,
Organism=Drosophila melanogaster, GI18859837, Length=281, Percent_Identity=49.4661921708185, Blast_Score=264, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=29.3650793650794, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356301, Length=188, Percent_Identity=26.5957446808511, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640492, Length=187, Percent_Identity=25.1336898395722, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI23397609, Length=191, Percent_Identity=30.8900523560209, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364892, Length=174, Percent_Identity=25.8620689655172, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357041, Length=195, Percent_Identity=27.1794871794872, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24644339, Length=189, Percent_Identity=26.4550264550265, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y0148_MYCTU (P96825)

Other databases:

- EMBL:   BX842572
- PIR:   G70617
- RefSeq:   NP_214662.1
- HSSP:   P97852
- ProteinModelPortal:   P96825
- SMR:   P96825
- EnsemblBacteria:   EBMYCT00000000084
- GeneID:   886845
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv0148
- TubercuList:   Rv0148
- GeneTree:   EBGT00050000015169
- HOGENOM:   HBG750976
- ProtClustDB:   CLSK790302
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081

Pfam domain/function: PF00106 adh_short

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 29779; Mature: 29648

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 164-164 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGVQDRVIVVTGAGGGLGREYALTLAGEGASVVVNDLGGARDGTGAGSAMADEVVAEIR
CCCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
DKGGRAVANYDSVATEDGAANIIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVL
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCEEE
KVHLYGGYHVLRAAWPHFREQSYGRVVVATSTSGLFGNFGQTNYGAAKLGLVGLINTLAL
EEEEECCCCEEHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EGAKYNIHANALAPIAATRMTQDILPPEVLEKLTPEFVAPVVAYLCTEECADNASVYVVG
CCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEE
GGKVQRVALFGNDGANFDKPPSVQDVAARWAEITDLSGAKIAGFKL
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCC
>Mature Secondary Structure 
PGVQDRVIVVTGAGGGLGREYALTLAGEGASVVVNDLGGARDGTGAGSAMADEVVAEIR
CCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
DKGGRAVANYDSVATEDGAANIIKTALDEFGAVHGVVSNAGILRDGTFHKMSFENWDAVL
HCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCEEE
KVHLYGGYHVLRAAWPHFREQSYGRVVVATSTSGLFGNFGQTNYGAAKLGLVGLINTLAL
EEEEECCCCEEHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EGAKYNIHANALAPIAATRMTQDILPPEVLEKLTPEFVAPVVAYLCTEECADNASVYVVG
CCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEE
GGKVQRVALFGNDGANFDKPPSVQDVAARWAEITDLSGAKIAGFKL
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230