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The map label for this gene is glyA [H]

Identifier: 148659830

GI number: 148659830

Start: 78977

End: 80254

Strand: Reverse

Name: glyA [H]

Synonym: MRA_0072

Alternate gene names: 148659830

Gene position: 80254-78977 (Counterclockwise)

Preceding gene: 148659836

Following gene: 148659829

Centisome position: 1.82

GC content: 66.12

Gene sequence:

>1278_bases
ATGAACACCCTCAACGACTCCCTGACCGCCTTCGACCCGGACATCGCCGCCCTGATCGACGGCGAGCTGCGCCGTCAAGA
ATCCGGCTTGGAGATGATCGCTTCGGAGAACTATGCACCGCTGGCCGTGATGCAGGCCCAAGGTTCGGTCTTGACCAACA
AGTACGCCGAAGGCTACCCGGGCCGGCGCTACTACGGTGGCTGTGAATTCGTCGACGGTGTCGAGCAGTTGGCTATCGAC
CGCGTCAAAGCGCTCTTTGGCGCCGAATACGCCAACGTGCAACCACATTCGGGGGCCACCGCCAACGCCGCCACCATGCA
TGCGCTGCTAAACCCCGGCGACACCATCCTGGGGTTGTCGCTGGCTCATGGCGGTCACCTGACCCACGGGATGCGGATCA
ACTTTTCCGGCAAGCTCTACCACGCCACCGCCTACGAGGTGTCCAAAGAGGACTACCTGGTCGACATGGATGCCGTCGCC
GAGGCAGCGCGCACACACCGGCCCAAAATGATCATCGCCGGCTGGTCGGCGTACCCACGCCAGCTGGATTTCGCCCGCTT
CCGCGCCATCGCCGACGAAGTCGACGCCGTGCTCATGGTGGATATGGCGCATTTCGCCGGCCTGGTCGCCGCTGGCGTGC
ACCCCAGCCCGGTGCCGCACGCCCACGTCGTCACCTCCACCACTCACAAGACGCTCGGCGGGCCCCGCGGCGGCATCATC
TTGTGCAATGACCCGGCCATCGCCAAGAAGATCAATTCCGCGGTCTTCCCTGGGCAGCAGGGCGGGCCGCTCGAGCATGT
CATCGCAGCCAAGGCCACCGCATTCAAGATGGCAGCACAACCTGAATTCGCGCAGCGCCAACAACGTTGCCTCGACGGCG
CGCGCATCCTTGCCGGCCGGTTGACCCAGCCCGACGTCGCCGAACGTGGCATCGCGGTGCTAACCGGCGGCACCGATGTG
CACCTCGTCCTAGTCGACCTGCGCGACGCCGAACTCGACGGCCAGCAAGCCGAAGACCGGTTGGCCGCCGTGGACATCAC
CGTCAACCGCAACGCGGTACCCTTCGACCCTCGTCCCCCGATGATCACCTCGGGCCTGCGAATCGGCACCCCGGCGCTGG
CCGCACGCGGCTTCTCCCACAACGACTTCCGCGCCGTGGCAGACCTCATCGCGGCGGCACTGACGGCCACCAACGACGAC
CAGCTGGGTCCGCTGCGCGCCCAGGTCCAGCGGCTGGCCGCACGCTATCCGCTCTACCCGGAACTGCACCGGACATGA

Upstream 100 bases:

>100_bases
CGATGTCGCGGCCGCCGGATGCCCATTTGGCCGGGCAGCCGATTGCTCCGGGAACACCCTTGACCGCCCCCGAGTACGCC
GAACAGACCGGACTGAGCTG

Downstream 100 bases:

>100_bases
CCATCAGCGTCTTCGACCTGTTCACCATCGGCATCGGGCCGTCCAGTTCCCACACCGTGGGACCGATGCGCGCGGCAAAC
CAGTTCGTAGTTGCGCTGCG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 2; Serine methylase 2 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNTLNDSLTAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYPGRRYYGGCEFVDGVEQLAID
RVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLSLAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVA
EAARTHRPKMIIAGWSAYPRQLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGII
LCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGRLTQPDVAERGIAVLTGGTDV
HLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPPMITSGLRIGTPALAARGFSHNDFRAVADLIAAALTATNDD
QLGPLRAQVQRLAARYPLYPELHRT

Sequences:

>Translated_425_residues
MNTLNDSLTAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYPGRRYYGGCEFVDGVEQLAID
RVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLSLAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVA
EAARTHRPKMIIAGWSAYPRQLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGII
LCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGRLTQPDVAERGIAVLTGGTDV
HLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPPMITSGLRIGTPALAARGFSHNDFRAVADLIAAALTATNDD
QLGPLRAQVQRLAARYPLYPELHRT
>Mature_425_residues
MNTLNDSLTAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYPGRRYYGGCEFVDGVEQLAID
RVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLSLAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVA
EAARTHRPKMIIAGWSAYPRQLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGII
LCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGRLTQPDVAERGIAVLTGGTDV
HLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPPMITSGLRIGTPALAARGFSHNDFRAVADLIAAALTATNDD
QLGPLRAQVQRLAARYPLYPELHRT

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=411, Percent_Identity=46.9586374695864, Blast_Score=335, Evalue=7e-92,
Organism=Homo sapiens, GI261862350, Length=411, Percent_Identity=46.9586374695864, Blast_Score=335, Evalue=7e-92,
Organism=Homo sapiens, GI261862348, Length=411, Percent_Identity=46.9586374695864, Blast_Score=335, Evalue=7e-92,
Organism=Homo sapiens, GI19923315, Length=411, Percent_Identity=46.9586374695864, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI261862346, Length=411, Percent_Identity=46.4720194647202, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI22547186, Length=409, Percent_Identity=46.2102689486553, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI22547189, Length=395, Percent_Identity=43.2911392405063, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=54.3269230769231, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25144732, Length=414, Percent_Identity=43.9613526570048, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25144729, Length=414, Percent_Identity=43.9613526570048, Blast_Score=321, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6319739, Length=417, Percent_Identity=42.6858513189448, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6323087, Length=414, Percent_Identity=39.6135265700483, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24640005, Length=413, Percent_Identity=47.4576271186441, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI221329721, Length=413, Percent_Identity=47.4576271186441, Blast_Score=351, Evalue=4e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45526; Mature: 45526

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLNDSLTAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYP
CCCCCCCCHHCCCCHHHHHCHHHHHHHCCHHHHHCCCCCCEEEEECCCCCHHHHHHCCCC
GRRYYGGCEFVDGVEQLAIDRVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLS
CCEECCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEE
LAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVAEAARTHRPKMIIAGWSAYPR
EECCCCCCCCEEEEECCEEEEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCC
QLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHCCCCCCCEE
LCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGR
EECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
LTQPDVAERGIAVLTGGTDVHLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPP
CCCCCHHHCCEEEEECCCEEEEEEEEECCCCCCCCHHCCCEEEEEEEECCCCCCCCCCCC
MITSGLRIGTPALAARGFSHNDFRAVADLIAAALTATNDDQLGPLRAQVQRLAARYPLYP
CEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
ELHRT
HHCCC
>Mature Secondary Structure
MNTLNDSLTAFDPDIAALIDGELRRQESGLEMIASENYAPLAVMQAQGSVLTNKYAEGYP
CCCCCCCCHHCCCCHHHHHCHHHHHHHCCHHHHHCCCCCCEEEEECCCCCHHHHHHCCCC
GRRYYGGCEFVDGVEQLAIDRVKALFGAEYANVQPHSGATANAATMHALLNPGDTILGLS
CCEECCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEEE
LAHGGHLTHGMRINFSGKLYHATAYEVSKEDYLVDMDAVAEAARTHRPKMIIAGWSAYPR
EECCCCCCCCEEEEECCEEEEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCC
QLDFARFRAIADEVDAVLMVDMAHFAGLVAAGVHPSPVPHAHVVTSTTHKTLGGPRGGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHCCCCCCCEE
LCNDPAIAKKINSAVFPGQQGGPLEHVIAAKATAFKMAAQPEFAQRQQRCLDGARILAGR
EECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
LTQPDVAERGIAVLTGGTDVHLVLVDLRDAELDGQQAEDRLAAVDITVNRNAVPFDPRPP
CCCCCHHHCCEEEEECCCEEEEEEEEECCCCCCCCHHCCCEEEEEEEECCCCCCCCCCCC
MITSGLRIGTPALAARGFSHNDFRAVADLIAAALTATNDDQLGPLRAQVQRLAARYPLYP
CEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
ELHRT
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]