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The map label for this gene is fadD34 [H]

Identifier: 148659795

GI number: 148659795

Start: 38617

End: 40305

Strand: Direct

Name: fadD34 [H]

Synonym: MRA_0038

Alternate gene names: 148659795

Gene position: 38617-40305 (Clockwise)

Preceding gene: 148659794

Following gene: 148659798

Centisome position: 0.87

GC content: 63.59

Gene sequence:

>1689_bases
ATGACGGCGGCCTTGCTTTCACCAGCCATCGCCTGGCAGCAGATCTCGGCTTGCACGGACCGCACGCTGACGATCACTTG
CGAGGATTCCGAGGTAATCAGCTATCAGGACCTCATCGCGCGCGCGGCGGCATGCATCCCCCCGCTACGGCGTCTTGACC
TCAAACGCGGTGAACCCGTGCTGATCACCGCCCACACCAACCTGGAATTCCTGTCCTGCTTTTTGGGCCTCATGCTCCAT
GGCGCTGTGCCGGTACCCATCCCGCCGCGGGAGGCACTGAAGACCACCGAGCGTTTCATGACTCGGCTCGGCCCACTGCT
GCGCCATCACCGCGTGCTGATCTGCACACCGGCCGAACACGACGAGATACGCGCTGCCGCCAGCACCGACTGCCAGATCA
GCAGATTTACTGCCCTAGCCGAGGCTGGCGACGAGCAGTTCGGCCGCGCCACGGCCCAGCAACTCGCCGACACCGCCACC
GCCGACTGGCCGCTATGCACCCTCGACGACGACGCCTACGTCCAATACACCTCTGGCAGCACCGCAGCACCACGCGGAGT
GGTCATCACCTACCGCAACCTGCTGTCCAACATGCGCGCAATGGCCGTGGGCTCACAATTCCAGCACGGCGATGTCATGG
GCAGCTGGCTGCCCTTGCACCATGACATGGGGCTGGTGGGCAGCCTATTCGCCGCACTCTTCAACAGTGTCAGCGCGGTA
TTCACCACGCCACACCGGTTTCTGTATGACCCGTTGGGATTCCTCAGACTGCTCACCAGCTCCGGGGCTACCCACACGTT
CATGCCTAACTTCGCTCTGGAGTGGCTGATCAACGCCTACCACAGGCGCGGCGCCGACATCGAAGGCATCGACCTACACA
AAATGCGCCGCTTGATCATCGCCTCCGAACCCGTCCATGCCGAGGGCATGCGGAGATTCGCCGCCACCTTCGCCGGCGTC
GGACTTGCCCCCACGGCCCTGGGTTCGGGCTATGGCCTGGCCGAAGCGACCGTCGCCGTGTCGATGTCAGCGCCCAACAC
GGGATTCCGCACCGAAACCCACGCCGCCGCGGAGGTCGTCACCGGCGGCCGAGTGCTGCCTGGCTACGAGGTGCGCATTG
ACGCCGCACCAGGTGCCCGGGCCGGAACGATCAAACTGCGCGGCGACAGCGTGGCCGCCAAAGCCTATGTGGGCGGGAAG
AAGCTGGACGCGCTCGACGAGGAAGGCTTCTGCGACACCCACGACTTGGGTTTTCTTGTAGACGACGAAATCGTCATCCT
TGGCCGGCAGGACGAGGTGTTCATTGTCCACGGAGAAAACAGATTCCCCTACGACATCGAGTTCATCATTCGCGGGGAAT
CCGAGCAGCACCGGACCAAAGTCGCATGTTTCGGGGTCAACGAACGCGTCGTGGTTGTGTTGGAAAGCCCATTGGACAGC
ATCATCGACAAGGCCGAAGCCGACCGACTGAGATGTCAAGTCGTTGCCGCGACTGGGCTGCAGTTGGATGAACTGATCAC
GGTTCGGCGCGGCGCGATTCCCACCACCACCAGCGGCAAGCTCAAACGACGCGCCGTCGCGCAGGCTTATCGAGACGGCA
CACTGCCCCGTCTTGCCACCCACGCGTGGACGGCGGATCCCGATAGCGCTCCCAAAACGACCCGGTCCAGCCTGGAAGGC
GCCCACTGA

Upstream 100 bases:

>100_bases
TCGACCAGCGCGAGGCGATCCTGGTTACCGTCGCCGGTGGTCGGATCACCCGACTTAGCGAGTTTTACGCCGACCCGGCG
GCGACCGAAAGCTTCTGGGC

Downstream 100 bases:

>100_bases
TCTTCCACTGACGTCTCATCAAACCCCCGGGGCGCTCGCGCGCTGGGCGCGCTCATCGACCGGGGCTTGGGTTGATTGGC
CCCGGCTCTCTTCGCGCGCT

Product: fatty-acid-CoA ligase FadD34

Products: [acyl carrier protein]; L-1-phosphatidyl-ethanolamine; AMP; pyrophosphate; acyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MTAALLSPAIAWQQISACTDRTLTITCEDSEVISYQDLIARAAACIPPLRRLDLKRGEPVLITAHTNLEFLSCFLGLMLH
GAVPVPIPPREALKTTERFMTRLGPLLRHHRVLICTPAEHDEIRAAASTDCQISRFTALAEAGDEQFGRATAQQLADTAT
ADWPLCTLDDDAYVQYTSGSTAAPRGVVITYRNLLSNMRAMAVGSQFQHGDVMGSWLPLHHDMGLVGSLFAALFNSVSAV
FTTPHRFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAYHRRGADIEGIDLHKMRRLIIASEPVHAEGMRRFAATFAGV
GLAPTALGSGYGLAEATVAVSMSAPNTGFRTETHAAAEVVTGGRVLPGYEVRIDAAPGARAGTIKLRGDSVAAKAYVGGK
KLDALDEEGFCDTHDLGFLVDDEIVILGRQDEVFIVHGENRFPYDIEFIIRGESEQHRTKVACFGVNERVVVVLESPLDS
IIDKAEADRLRCQVVAATGLQLDELITVRRGAIPTTTSGKLKRRAVAQAYRDGTLPRLATHAWTADPDSAPKTTRSSLEG
AH

Sequences:

>Translated_562_residues
MTAALLSPAIAWQQISACTDRTLTITCEDSEVISYQDLIARAAACIPPLRRLDLKRGEPVLITAHTNLEFLSCFLGLMLH
GAVPVPIPPREALKTTERFMTRLGPLLRHHRVLICTPAEHDEIRAAASTDCQISRFTALAEAGDEQFGRATAQQLADTAT
ADWPLCTLDDDAYVQYTSGSTAAPRGVVITYRNLLSNMRAMAVGSQFQHGDVMGSWLPLHHDMGLVGSLFAALFNSVSAV
FTTPHRFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAYHRRGADIEGIDLHKMRRLIIASEPVHAEGMRRFAATFAGV
GLAPTALGSGYGLAEATVAVSMSAPNTGFRTETHAAAEVVTGGRVLPGYEVRIDAAPGARAGTIKLRGDSVAAKAYVGGK
KLDALDEEGFCDTHDLGFLVDDEIVILGRQDEVFIVHGENRFPYDIEFIIRGESEQHRTKVACFGVNERVVVVLESPLDS
IIDKAEADRLRCQVVAATGLQLDELITVRRGAIPTTTSGKLKRRAVAQAYRDGTLPRLATHAWTADPDSAPKTTRSSLEG
AH
>Mature_561_residues
TAALLSPAIAWQQISACTDRTLTITCEDSEVISYQDLIARAAACIPPLRRLDLKRGEPVLITAHTNLEFLSCFLGLMLHG
AVPVPIPPREALKTTERFMTRLGPLLRHHRVLICTPAEHDEIRAAASTDCQISRFTALAEAGDEQFGRATAQQLADTATA
DWPLCTLDDDAYVQYTSGSTAAPRGVVITYRNLLSNMRAMAVGSQFQHGDVMGSWLPLHHDMGLVGSLFAALFNSVSAVF
TTPHRFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAYHRRGADIEGIDLHKMRRLIIASEPVHAEGMRRFAATFAGVG
LAPTALGSGYGLAEATVAVSMSAPNTGFRTETHAAAEVVTGGRVLPGYEVRIDAAPGARAGTIKLRGDSVAAKAYVGGKK
LDALDEEGFCDTHDLGFLVDDEIVILGRQDEVFIVHGENRFPYDIEFIIRGESEQHRTKVACFGVNERVVVVLESPLDSI
IDKAEADRLRCQVVAATGLQLDELITVRRGAIPTTTSGKLKRRAVAQAYRDGTLPRLATHAWTADPDSAPKTTRSSLEGA
H

Specific function: Plays A Role In Lysophospholipid Acylation. Transfers Fatty Acids To The 1-Position VIa An Enzyme-Bound Acyl-Acp Intermediate In The Presence Of ATP And Mg(2+). Its Physiological Function Is To Regenerate Ptdetn From 2-Acyl-Gpe Formed By Transacylation R

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789201, Length=290, Percent_Identity=26.8965517241379, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=449, Percent_Identity=24.9443207126949, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 60962; Mature: 60831

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALLSPAIAWQQISACTDRTLTITCEDSEVISYQDLIARAAACIPPLRRLDLKRGEPV
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEE
LITAHTNLEFLSCFLGLMLHGAVPVPIPPREALKTTERFMTRLGPLLRHHRVLICTPAEH
EEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DEIRAAASTDCQISRFTALAEAGDEQFGRATAQQLADTATADWPLCTLDDDAYVQYTSGS
CHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCC
TAAPRGVVITYRNLLSNMRAMAVGSQFQHGDVMGSWLPLHHDMGLVGSLFAALFNSVSAV
CCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
FTTPHRFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAYHRRGADIEGIDLHKMRRLII
HCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ASEPVHAEGMRRFAATFAGVGLAPTALGSGYGLAEATVAVSMSAPNTGFRTETHAAAEVV
CCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
TGGRVLPGYEVRIDAAPGARAGTIKLRGDSVAAKAYVGGKKLDALDEEGFCDTHDLGFLV
CCCEECCCEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEE
DDEIVILGRQDEVFIVHGENRFPYDIEFIIRGESEQHRTKVACFGVNERVVVVLESPLDS
CCCEEEEECCCCEEEEECCCCCCEEEEEEEECCCHHHCCEEEEEECCCEEEEEECCCHHH
IIDKAEADRLRCQVVAATGLQLDELITVRRGAIPTTTSGKLKRRAVAQAYRDGTLPRLAT
HHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
HAWTADPDSAPKTTRSSLEGAH
HCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
TAALLSPAIAWQQISACTDRTLTITCEDSEVISYQDLIARAAACIPPLRRLDLKRGEPV
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEE
LITAHTNLEFLSCFLGLMLHGAVPVPIPPREALKTTERFMTRLGPLLRHHRVLICTPAEH
EEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DEIRAAASTDCQISRFTALAEAGDEQFGRATAQQLADTATADWPLCTLDDDAYVQYTSGS
CHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCC
TAAPRGVVITYRNLLSNMRAMAVGSQFQHGDVMGSWLPLHHDMGLVGSLFAALFNSVSAV
CCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
FTTPHRFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAYHRRGADIEGIDLHKMRRLII
HCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ASEPVHAEGMRRFAATFAGVGLAPTALGSGYGLAEATVAVSMSAPNTGFRTETHAAAEVV
CCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
TGGRVLPGYEVRIDAAPGARAGTIKLRGDSVAAKAYVGGKKLDALDEEGFCDTHDLGFLV
CCCEECCCEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEE
DDEIVILGRQDEVFIVHGENRFPYDIEFIIRGESEQHRTKVACFGVNERVVVVLESPLDS
CCCEEEEECCCCEEEEECCCCCCEEEEEEEECCCHHHCCEEEEEECCCEEEEEECCCHHH
IIDKAEADRLRCQVVAATGLQLDELITVRRGAIPTTTSGKLKRRAVAQAYRDGTLPRLAT
HHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
HAWTADPDSAPKTTRSSLEGAH
HCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-ACP; 2-acyl-sn-glycero-3-phosphoethanolamine; ATP; a fatty acid [C]

Specific reaction: acyl-ACP + 2-acyl-sn-glycero-3-phosphoethanolamine = [acyl carrier protein] + L-1-phosphatidyl-ethanolamine ATP + a fatty acid + [acyl carrier protein] = AMP + pyrophosphate + acyl-ACP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]