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The map label for this gene is gcvH

Identifier: 148653520

GI number: 148653520

Start: 2131148

End: 2131528

Strand: Direct

Name: gcvH

Synonym: PsycPRwf_1723

Alternate gene names: 148653520

Gene position: 2131148-2131528 (Clockwise)

Preceding gene: 148653519

Following gene: 148653521

Centisome position: 71.54

GC content: 47.77

Gene sequence:

>381_bases
ATGAGTAATGTTCCATCAGAGCTAAAGTACGTTGCCAGCCATGAGTGGCTACGTTTGGAAGACGACGGTACCGTGACCGT
TGGTATCACTGACCACGCTCAAGAAGCCCTTGGCGATATCGTTTATGTTGAACTACCAGACGTCGGTGACACTGTGGCCG
TTGACGACGAAGTGGCTGTCGTTGAGTCTGTAAAAGCCGCCTCTGACGTTTATGCCCCGCTTACTGGTGAAGTTGTGGCG
ATTAACGAAGCGCTTGAAGATGATCCTGAAGTTATCAATACCGACCCATACGGTGAAGGTTGGATGTATCGTATCAAGCC
TGACAATGCTGATGACTTTGAGTCATTATTATCAGCTGAAGAGTACCAAGCTGAACTATAA

Upstream 100 bases:

>100_bases
AGTAAGAGCGAATTTGCCCCAGTAGCGGATTTATTTAACCTTTTAATTCAACTACTTAATATTTTATTATTGTTTGATTT
TTTAACCCCAGGAGAAAAAC

Downstream 100 bases:

>100_bases
GTAACGCTTTATTGTCCATCAGTGTGTTAGATCAGCATTGCCTCTGACATGAGGTAATGCTGAGTTTTAAGACTTATTTT
ATAGTGCTTAAAATATAGAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSNVPSELKYVASHEWLRLEDDGTVTVGITDHAQEALGDIVYVELPDVGDTVAVDDEVAVVESVKAASDVYAPLTGEVVA
INEALEDDPEVINTDPYGEGWMYRIKPDNADDFESLLSAEEYQAEL

Sequences:

>Translated_126_residues
MSNVPSELKYVASHEWLRLEDDGTVTVGITDHAQEALGDIVYVELPDVGDTVAVDDEVAVVESVKAASDVYAPLTGEVVA
INEALEDDPEVINTDPYGEGWMYRIKPDNADDFESLLSAEEYQAEL
>Mature_125_residues
SNVPSELKYVASHEWLRLEDDGTVTVGITDHAQEALGDIVYVELPDVGDTVAVDDEVAVVESVKAASDVYAPLTGEVVAI
NEALEDDPEVINTDPYGEGWMYRIKPDNADDFESLLSAEEYQAEL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=46.0869565217391, Blast_Score=114, Evalue=3e-26,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=45.2173913043478, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=61.1111111111111, Blast_Score=165, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=48.6725663716814, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=44.2477876106195, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=41.40625, Blast_Score=112, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=39.2, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PSYWF (A5WG72)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280613.1
- ProteinModelPortal:   A5WG72
- SMR:   A5WG72
- STRING:   A5WG72
- GeneID:   5206196
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1723
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   PSP56811:PSYCPRWF_1723-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13760; Mature: 13629

Theoretical pI: Translated: 3.51; Mature: 3.51

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPSELKYVASHEWLRLEDDGTVTVGITDHAQEALGDIVYVELPDVGDTVAVDDEVAV
CCCCCCHHHHHHCCCEEEECCCCEEEEEECHHHHHHHCCEEEEEECCCCCEEEECCCHHH
VESVKAASDVYAPLTGEVVAINEALEDDPEVINTDPYGEGWMYRIKPDNADDFESLLSAE
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCEEEECCCCCHHHHHHHCHH
EYQAEL
HHHCCC
>Mature Secondary Structure 
SNVPSELKYVASHEWLRLEDDGTVTVGITDHAQEALGDIVYVELPDVGDTVAVDDEVAV
CCCCCHHHHHHCCCEEEECCCCEEEEEECHHHHHHHCCEEEEEECCCCCEEEECCCHHH
VESVKAASDVYAPLTGEVVAINEALEDDPEVINTDPYGEGWMYRIKPDNADDFESLLSAE
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCEEEECCCCCHHHHHHHCHH
EYQAEL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA