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The map label for this gene is prfB [H]

Identifier: 148652647

GI number: 148652647

Start: 994586

End: 995563

Strand: Direct

Name: prfB [H]

Synonym: PsycPRwf_0838

Alternate gene names: 148652647

Gene position: 994586-995563 (Clockwise)

Preceding gene: 148652646

Following gene: 148652648

Centisome position: 33.39

GC content: 48.57

Gene sequence:

>978_bases
ATGGCGTCGCCAGAGCTTTGGAATGACCCAGAGCGCGCGACCAAAATCAGCAAAGAAAAAAGCCAGCTTGATGCAGTGAT
TACTACCATTACCGGTCTAGACACTACTCTTGAAGATGCCGCTGCCATGCTTGAGCTGGCGGTAGAGGCCGATGATGAAG
GCTTGCTTGAAGAGGTGCAAAATGAGCTTAATGCTGCCCAAGCTTTGGTAGAGGACTTAGAGTTTAAGCGCATGTTCGAT
GGCGAGATGGATGCCAATAACTGCTTCGTTGATATTCAATCAGGCAGTGGGGGTACCGAGGCGCAAGACTGGGCAGAGAT
GCTGCTACGCATGTATACCCGTTGGTGTGAGTCACATGGGTTTAAGGTTGAGATTATTGAGGTGTCAGCGGGTAGTGTGG
CTGGCGTGAAATCAGCCTCATTTAAAGTCAGCGGTGATTATGCCTTTGGCTGGCTGCGAACCGAGACAGGCGTACATCGC
TTGGTGCGCAAATCACCCTTTGATAGCAATAATGGCCGTCATACCTCGTTTGCTGCGGTGTTTGTGTCGCCTGAAATTGA
TGATGATTTTGAGATTGATATTAATCCGGCTGATTTACGTATTGATACCTATCGCTCAAGTGGGGCAGGTGGTCAACACG
TCAACACCACCGACTCTGCGGTGCGTATTACCCACGTACCGACGGGTACCGTGGTGCAGTGTCAAAATGAGCGCAGCCAA
CACGGCAACAAAGCCACGGCCATGAAGATGCTACGCGCTAAATTGTATGAGCTTGAGATGCAAAAGCGTATGGAGCAGCA
GCAAGCCGTTGAAGACAATAAATCTGATGTGGGCTGGGGCAGTCAAATTCGCTCGTATGTGCTTGATGACTCTCGTATTA
AAGATTTACGTACTGGTGTTGAGACCTTTAATACCGGTGCAGTATTAGATGGTGATTTGGATCAATTTATTGAGGCAAGT
CTTAAAGCAGGATTATAA

Upstream 100 bases:

>100_bases
CAAGAACAAATCAAAGATTTAAGCGAGCGCTCACAGGACATGCGGAGGTATCTTTGACTTCGATGGTAAGTCAGAGCGCC
TAGAGGAGGTTAATCTTGAG

Downstream 100 bases:

>100_bases
ACAGGCTCATCAGGCCCTAGGCGAACAGCAGTAAACAGGTACTATTCTTAAACAATTCTTAAACATTTTTTATTTGGTAA
AATTTTACTTATCAATGTAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MASPELWNDPERATKISKEKSQLDAVITTITGLDTTLEDAAAMLELAVEADDEGLLEEVQNELNAAQALVEDLEFKRMFD
GEMDANNCFVDIQSGSGGTEAQDWAEMLLRMYTRWCESHGFKVEIIEVSAGSVAGVKSASFKVSGDYAFGWLRTETGVHR
LVRKSPFDSNNGRHTSFAAVFVSPEIDDDFEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTGTVVQCQNERSQ
HGNKATAMKMLRAKLYELEMQKRMEQQQAVEDNKSDVGWGSQIRSYVLDDSRIKDLRTGVETFNTGAVLDGDLDQFIEAS
LKAGL

Sequences:

>Translated_325_residues
MASPELWNDPERATKISKEKSQLDAVITTITGLDTTLEDAAAMLELAVEADDEGLLEEVQNELNAAQALVEDLEFKRMFD
GEMDANNCFVDIQSGSGGTEAQDWAEMLLRMYTRWCESHGFKVEIIEVSAGSVAGVKSASFKVSGDYAFGWLRTETGVHR
LVRKSPFDSNNGRHTSFAAVFVSPEIDDDFEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTGTVVQCQNERSQ
HGNKATAMKMLRAKLYELEMQKRMEQQQAVEDNKSDVGWGSQIRSYVLDDSRIKDLRTGVETFNTGAVLDGDLDQFIEAS
LKAGL
>Mature_324_residues
ASPELWNDPERATKISKEKSQLDAVITTITGLDTTLEDAAAMLELAVEADDEGLLEEVQNELNAAQALVEDLEFKRMFDG
EMDANNCFVDIQSGSGGTEAQDWAEMLLRMYTRWCESHGFKVEIIEVSAGSVAGVKSASFKVSGDYAFGWLRTETGVHRL
VRKSPFDSNNGRHTSFAAVFVSPEIDDDFEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTGTVVQCQNERSQH
GNKATAMKMLRAKLYELEMQKRMEQQQAVEDNKSDVGWGSQIRSYVLDDSRIKDLRTGVETFNTGAVLDGDLDQFIEASL
KAGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=34.4051446945338, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI34577120, Length=323, Percent_Identity=34.3653250773994, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=34.1121495327103, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI2367172, Length=325, Percent_Identity=69.2307692307692, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1787462, Length=294, Percent_Identity=40.8163265306122, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=335, Percent_Identity=31.044776119403, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=248, Percent_Identity=39.1129032258064, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI19921226, Length=227, Percent_Identity=42.2907488986784, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35886; Mature: 35755

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPELWNDPERATKISKEKSQLDAVITTITGLDTTLEDAAAMLELAVEADDEGLLEEVQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
NELNAAQALVEDLEFKRMFDGEMDANNCFVDIQSGSGGTEAQDWAEMLLRMYTRWCESHG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
FKVEIIEVSAGSVAGVKSASFKVSGDYAFGWLRTETGVHRLVRKSPFDSNNGRHTSFAAV
CEEEEEEECCCCCCCCCCCCEEECCCEEEEEEECHHHHHHHHHCCCCCCCCCCEEEEEEE
FVSPEIDDDFEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTGTVVQCQNERSQ
EECCCCCCCCEEECCHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCHHHH
HGNKATAMKMLRAKLYELEMQKRMEQQQAVEDNKSDVGWGSQIRSYVLDDSRIKDLRTGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
ETFNTGAVLDGDLDQFIEASLKAGL
HHCCCCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ASPELWNDPERATKISKEKSQLDAVITTITGLDTTLEDAAAMLELAVEADDEGLLEEVQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
NELNAAQALVEDLEFKRMFDGEMDANNCFVDIQSGSGGTEAQDWAEMLLRMYTRWCESHG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
FKVEIIEVSAGSVAGVKSASFKVSGDYAFGWLRTETGVHRLVRKSPFDSNNGRHTSFAAV
CEEEEEEECCCCCCCCCCCCEEECCCEEEEEEECHHHHHHHHHCCCCCCCCCCEEEEEEE
FVSPEIDDDFEIDINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTGTVVQCQNERSQ
EECCCCCCCCEEECCHHHEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCHHHH
HGNKATAMKMLRAKLYELEMQKRMEQQQAVEDNKSDVGWGSQIRSYVLDDSRIKDLRTGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
ETFNTGAVLDGDLDQFIEASLKAGL
HHCCCCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA