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The map label for this gene is tilS

Identifier: 148652148

GI number: 148652148

Start: 405792

End: 407369

Strand: Direct

Name: tilS

Synonym: PsycPRwf_0336

Alternate gene names: 148652148

Gene position: 405792-407369 (Clockwise)

Preceding gene: 148652147

Following gene: 148652149

Centisome position: 13.62

GC content: 50.51

Gene sequence:

>1578_bases
ATGACTACCCCTGTCTGTATTTCACAGGCCTTTATGGACAGCTTTCATGCCCATCACGCACAGCTACTTGGTAAACGGGT
TTGGCTGGCTTGTAGTGGGGGGCGTGACTCTTTAGGACTGGCGCTATTGTGCCGTACTTTGTTCGATCAAGGCCGGCTGC
CTTTTTTGCCGCAGCTCATTCATGTCAATCATGGGATGCAAGCTGCCAATGATGAGTGGGCGCAGCAAGTGGCTCAGTGG
GCACAGCAGCATGATATGGCTTGCCAAATTATTGGTCTTAATTTAACTCATAAAAGTGAGCAAGCAGCGCGCGATGGTCG
CTATCAAGCCATGATGCAGCTGATGAATCAAGATGATGTCTTAATACTGGGCCATCATCAAGATGATCAGGTCGAGACTT
TATTGATGCGGCTGTTTAATGGAGCCGGGGTGACGGGTTTGGGCGCCATGCGTGAGTGGACCTCAAAGCAGGCTCATACT
GCGCAGTCGCCACCAGATGTTAATAAGCCGCGTCAGCGCATATTTTTGTGGCGGCCGTGGCTAGAGATTAGCCGAGATCA
GATTACTGAATATGCCCAGCGCCACAATCTAAAGTATATCGATGACCCGACCAATGTGGCACAGTCGCCATCAAAGCTTG
CTTTGCAGACGCTAAATGATAGAGCTTGGCTGCGCAGTGTGTTGTTGCCGCATATCACTGAGCGCTACCCGCAAGCATCT
GAGGCCATGGCGCGTACGGCGCAGCTTATGCAGCAGGCCAGTGACAGTATTGATGAGCAAGTGACGCAGGATTTGGCGCA
GGTGGCTTTAGCCGCTACTGAGCAGCAGTCTGTGATTGCGCTAGATAAGCTGGCAGGGTTATCTGCACCACGGCAAGCGG
CGCTTATTCATCACTGGCTCGCCCCGTACCCTAATCAATTGCCGCCCTCAAAGCGCCTAGTGGATGAGGTGCTTGCACTT
AGCTTTCGCCAAGACAGCAACCACCAAACTTGTCTTTATTTCGATGCTGGCAGTGAGCAGTATCAAGTACGCCGTTATCA
AAATAAACTGTACCGCTTACAGCACGCCTATGCCCAGTGGCTACAGATGATGCCGCATCAAATACACTTACCGCTGGCGC
ACAATGCTGAGGAGCTATCTTTAAACCTAGCAGATACCGATGTTTTATCTTTAAAGCAGTCGGGGCTAGAGTTTGATTGG
CAATTAACTGGGGTGAGGGGGTTGATGGCACATTTAGCCCGTTTGCTAAATAGCGCGGATGCTAAAGTAACCCCCTGCCA
GCTTATCTTTGAGCCCTTGCCTCGGACAATTAAACTGGCTCTGGCGGGGCGTAGTGGGCGTAAGTCGGGCAAAAAGCTGC
TACAAGCTTTAGATCAGCCAAGCTTTATGCGTGGCTCTGTGGTGCTGTGCCGCTTAGATATGATGGGCTCTGATGGTATC
TTGCAGGACTCATCCACCGCTGTCCCCTTATTTATTATCTGTATCGATAGAATTTGGGTGCTGCAAAGTCAGTTTACCGC
TTTAATCAATCAGCTTCTGGCCACTGAGGTTCTAAGCACTCAGATTTTAGAGTGTTAA

Upstream 100 bases:

>100_bases
AGCCGTTATTTTAATACGTTACTGTTTGCAGATTAGTTTTTTGTAGAGCAGCGCTTGCACTGCAATGGTTGACAGACTGG
CTAAGAGGCCGCAATTTATT

Downstream 100 bases:

>100_bases
TGTCTAAATAGCTGATGTCTAAACGTCAAAGTTTTAAGTGCTTAAATAAAATCATATCTGCCTAACTTAAGTGCGAAACC
ATAACCCCATCTACTCATTA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTTPVCISQAFMDSFHAHHAQLLGKRVWLACSGGRDSLGLALLCRTLFDQGRLPFLPQLIHVNHGMQAANDEWAQQVAQW
AQQHDMACQIIGLNLTHKSEQAARDGRYQAMMQLMNQDDVLILGHHQDDQVETLLMRLFNGAGVTGLGAMREWTSKQAHT
AQSPPDVNKPRQRIFLWRPWLEISRDQITEYAQRHNLKYIDDPTNVAQSPSKLALQTLNDRAWLRSVLLPHITERYPQAS
EAMARTAQLMQQASDSIDEQVTQDLAQVALAATEQQSVIALDKLAGLSAPRQAALIHHWLAPYPNQLPPSKRLVDEVLAL
SFRQDSNHQTCLYFDAGSEQYQVRRYQNKLYRLQHAYAQWLQMMPHQIHLPLAHNAEELSLNLADTDVLSLKQSGLEFDW
QLTGVRGLMAHLARLLNSADAKVTPCQLIFEPLPRTIKLALAGRSGRKSGKKLLQALDQPSFMRGSVVLCRLDMMGSDGI
LQDSSTAVPLFIICIDRIWVLQSQFTALINQLLATEVLSTQILEC

Sequences:

>Translated_525_residues
MTTPVCISQAFMDSFHAHHAQLLGKRVWLACSGGRDSLGLALLCRTLFDQGRLPFLPQLIHVNHGMQAANDEWAQQVAQW
AQQHDMACQIIGLNLTHKSEQAARDGRYQAMMQLMNQDDVLILGHHQDDQVETLLMRLFNGAGVTGLGAMREWTSKQAHT
AQSPPDVNKPRQRIFLWRPWLEISRDQITEYAQRHNLKYIDDPTNVAQSPSKLALQTLNDRAWLRSVLLPHITERYPQAS
EAMARTAQLMQQASDSIDEQVTQDLAQVALAATEQQSVIALDKLAGLSAPRQAALIHHWLAPYPNQLPPSKRLVDEVLAL
SFRQDSNHQTCLYFDAGSEQYQVRRYQNKLYRLQHAYAQWLQMMPHQIHLPLAHNAEELSLNLADTDVLSLKQSGLEFDW
QLTGVRGLMAHLARLLNSADAKVTPCQLIFEPLPRTIKLALAGRSGRKSGKKLLQALDQPSFMRGSVVLCRLDMMGSDGI
LQDSSTAVPLFIICIDRIWVLQSQFTALINQLLATEVLSTQILEC
>Mature_524_residues
TTPVCISQAFMDSFHAHHAQLLGKRVWLACSGGRDSLGLALLCRTLFDQGRLPFLPQLIHVNHGMQAANDEWAQQVAQWA
QQHDMACQIIGLNLTHKSEQAARDGRYQAMMQLMNQDDVLILGHHQDDQVETLLMRLFNGAGVTGLGAMREWTSKQAHTA
QSPPDVNKPRQRIFLWRPWLEISRDQITEYAQRHNLKYIDDPTNVAQSPSKLALQTLNDRAWLRSVLLPHITERYPQASE
AMARTAQLMQQASDSIDEQVTQDLAQVALAATEQQSVIALDKLAGLSAPRQAALIHHWLAPYPNQLPPSKRLVDEVLALS
FRQDSNHQTCLYFDAGSEQYQVRRYQNKLYRLQHAYAQWLQMMPHQIHLPLAHNAEELSLNLADTDVLSLKQSGLEFDWQ
LTGVRGLMAHLARLLNSADAKVTPCQLIFEPLPRTIKLALAGRSGRKSGKKLLQALDQPSFMRGSVVLCRLDMMGSDGIL
QDSSTAVPLFIICIDRIWVLQSQFTALINQLLATEVLSTQILEC

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=355, Percent_Identity=29.5774647887324, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PSYWF (A5WCA0)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279241.1
- ProteinModelPortal:   A5WCA0
- SMR:   A5WCA0
- STRING:   A5WCA0
- GeneID:   5206021
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0336
- eggNOG:   COG0037
- HOGENOM:   HBG645109
- OMA:   WLACSGG
- ProtClustDB:   CLSK838842
- BioCyc:   PSP56811:PSYCPRWF_0336-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C

EC number: NA

Molecular weight: Translated: 59125; Mature: 58993

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPVCISQAFMDSFHAHHAQLLGKRVWLACSGGRDSLGLALLCRTLFDQGRLPFLPQLI
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHH
HVNHGMQAANDEWAQQVAQWAQQHDMACQIIGLNLTHKSEQAARDGRYQAMMQLMNQDDV
HHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHCCCCE
LILGHHQDDQVETLLMRLFNGAGVTGLGAMREWTSKQAHTAQSPPDVNKPRQRIFLWRPW
EEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEECCH
LEISRDQITEYAQRHNLKYIDDPTNVAQSPSKLALQTLNDRAWLRSVLLPHITERYPQAS
HHHCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EAMARTAQLMQQASDSIDEQVTQDLAQVALAATEQQSVIALDKLAGLSAPRQAALIHHWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
APYPNQLPPSKRLVDEVLALSFRQDSNHQTCLYFDAGSEQYQVRRYQNKLYRLQHAYAQW
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LQMMPHQIHLPLAHNAEELSLNLADTDVLSLKQSGLEFDWQLTGVRGLMAHLARLLNSAD
HHHCCCCEECCCCCCHHHCEEECCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCC
AKVTPCQLIFEPLPRTIKLALAGRSGRKSGKKLLQALDQPSFMRGSVVLCRLDMMGSDGI
CCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCC
LQDSSTAVPLFIICIDRIWVLQSQFTALINQLLATEVLSTQILEC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPVCISQAFMDSFHAHHAQLLGKRVWLACSGGRDSLGLALLCRTLFDQGRLPFLPQLI
CCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHH
HVNHGMQAANDEWAQQVAQWAQQHDMACQIIGLNLTHKSEQAARDGRYQAMMQLMNQDDV
HHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHCCCCE
LILGHHQDDQVETLLMRLFNGAGVTGLGAMREWTSKQAHTAQSPPDVNKPRQRIFLWRPW
EEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEECCH
LEISRDQITEYAQRHNLKYIDDPTNVAQSPSKLALQTLNDRAWLRSVLLPHITERYPQAS
HHHCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EAMARTAQLMQQASDSIDEQVTQDLAQVALAATEQQSVIALDKLAGLSAPRQAALIHHWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
APYPNQLPPSKRLVDEVLALSFRQDSNHQTCLYFDAGSEQYQVRRYQNKLYRLQHAYAQW
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LQMMPHQIHLPLAHNAEELSLNLADTDVLSLKQSGLEFDWQLTGVRGLMAHLARLLNSAD
HHHCCCCEECCCCCCHHHCEEECCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCC
AKVTPCQLIFEPLPRTIKLALAGRSGRKSGKKLLQALDQPSFMRGSVVLCRLDMMGSDGI
CCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCC
LQDSSTAVPLFIICIDRIWVLQSQFTALINQLLATEVLSTQILEC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA