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The map label for this gene is trmD

Identifier: 148652018

GI number: 148652018

Start: 238761

End: 239522

Strand: Direct

Name: trmD

Synonym: PsycPRwf_0202

Alternate gene names: 148652018

Gene position: 238761-239522 (Clockwise)

Preceding gene: 148652017

Following gene: 148652019

Centisome position: 8.01

GC content: 47.51

Gene sequence:

>762_bases
ATGTATTTCGCTGTCATCACCATTTTACCTGAGATGTTTACCGCGATAAGTGAGTATGGCATCACCGGTAGAGCGGTTCA
AAATAAGCAAGTGGTTATCGACTGCATAAATCCTCGCGATTTTACGACAGACAATTATCGCCGTATCGATGAGCGCCCTT
TTGGTGGTGGTCCGGGTATGGTGATGATGGCAGAACCTTTGGCAAAAGCCATAGCGTATGCCAAGTCGCAAGCAAGCTTA
CACGGCTGTATCACAGATTCAGAGCACTGTCCGGTTGTCTATCTGTCTCCGCAAGGCGATAAATTAGATGAAGCTGCAGT
GGTTTCTATGCGTCATTATGAGGCTATGATTCTGTTGTGTGGTCGCTATGAAGGCATTGATGAGCGACTGTTAGCGCGTT
ATGTTGATATGGAGATCTCTATTGGAGATTTTGTATTGACCGGTGGTGAGCTACCTGCGATGGTATTGATGGACAGTGTC
ATACGCCGATTGCCCGATGTTATGGGTGATGCAAAGTCGGCTGAGCAAGACTCCTTTGTTGAAGGGTTGCTAGATTGTCC
GCACTATACCAAGCCTCATGACTTTGAGGGGCTACAAGTGCCAGAGGTGTTATTATCTGGACACCATGCCAATATCGCAA
CGTGGCGGTTTATGCAGCAAGTTGAGCGCACCAAACAAAGGCGTCCTGACTTGTGGCAATCCTTTACCCCAAACGCCGAG
CAAGCCCGCTTGCTCAAAAAAGTTAACAAAACGATGAAGTAG

Upstream 100 bases:

>100_bases
TGATTTTTAAGCCCATAATTTAGATTAAGGCTTAACAATGATTCCTAGTTATTCAACGATTGTCTGTATTTTGACGCATT
AATAAGATGGAGGTGACCTA

Downstream 100 bases:

>100_bases
AGAGCCGATAGAACTTTCAATATGCTTTGTCTAAGTGCTAACTTTACTGTTTTTTATTATCCCAGATTCTAAACTAGCTG
GCTAAACCCGATGATAAACC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MYFAVITILPEMFTAISEYGITGRAVQNKQVVIDCINPRDFTTDNYRRIDERPFGGGPGMVMMAEPLAKAIAYAKSQASL
HGCITDSEHCPVVYLSPQGDKLDEAAVVSMRHYEAMILLCGRYEGIDERLLARYVDMEISIGDFVLTGGELPAMVLMDSV
IRRLPDVMGDAKSAEQDSFVEGLLDCPHYTKPHDFEGLQVPEVLLSGHHANIATWRFMQQVERTKQRRPDLWQSFTPNAE
QARLLKKVNKTMK

Sequences:

>Translated_253_residues
MYFAVITILPEMFTAISEYGITGRAVQNKQVVIDCINPRDFTTDNYRRIDERPFGGGPGMVMMAEPLAKAIAYAKSQASL
HGCITDSEHCPVVYLSPQGDKLDEAAVVSMRHYEAMILLCGRYEGIDERLLARYVDMEISIGDFVLTGGELPAMVLMDSV
IRRLPDVMGDAKSAEQDSFVEGLLDCPHYTKPHDFEGLQVPEVLLSGHHANIATWRFMQQVERTKQRRPDLWQSFTPNAE
QARLLKKVNKTMK
>Mature_253_residues
MYFAVITILPEMFTAISEYGITGRAVQNKQVVIDCINPRDFTTDNYRRIDERPFGGGPGMVMMAEPLAKAIAYAKSQASL
HGCITDSEHCPVVYLSPQGDKLDEAAVVSMRHYEAMILLCGRYEGIDERLLARYVDMEISIGDFVLTGGELPAMVLMDSV
IRRLPDVMGDAKSAEQDSFVEGLLDCPHYTKPHDFEGLQVPEVLLSGHHANIATWRFMQQVERTKQRRPDLWQSFTPNAE
QARLLKKVNKTMK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=247, Percent_Identity=53.8461538461538, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PSYWF (A5WBX0)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279111.1
- ProteinModelPortal:   A5WBX0
- SMR:   A5WBX0
- STRING:   A5WBX0
- GeneID:   5204786
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0202
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   PSP56811:PSYCPRWF_0202-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28486; Mature: 28486

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFAVITILPEMFTAISEYGITGRAVQNKQVVIDCINPRDFTTDNYRRIDERPFGGGPGM
CEEEHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCE
VMMAEPLAKAIAYAKSQASLHGCITDSEHCPVVYLSPQGDKLDEAAVVSMRHYEAMILLC
EEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHEE
GRYEGIDERLLARYVDMEISIGDFVLTGGELPAMVLMDSVIRRLPDVMGDAKSAEQDSFV
CCCCCHHHHHHHHHHCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EGLLDCPHYTKPHDFEGLQVPEVLLSGHHANIATWRFMQQVERTKQRRPDLWQSFTPNAE
HHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
QARLLKKVNKTMK
HHHHHHHHHHHCC
>Mature Secondary Structure
MYFAVITILPEMFTAISEYGITGRAVQNKQVVIDCINPRDFTTDNYRRIDERPFGGGPGM
CEEEHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCE
VMMAEPLAKAIAYAKSQASLHGCITDSEHCPVVYLSPQGDKLDEAAVVSMRHYEAMILLC
EEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHEE
GRYEGIDERLLARYVDMEISIGDFVLTGGELPAMVLMDSVIRRLPDVMGDAKSAEQDSFV
CCCCCHHHHHHHHHHCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EGLLDCPHYTKPHDFEGLQVPEVLLSGHHANIATWRFMQQVERTKQRRPDLWQSFTPNAE
HHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
QARLLKKVNKTMK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA