Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 148651992

GI number: 148651992

Start: 211558

End: 213015

Strand: Direct

Name: murD [H]

Synonym: PsycPRwf_0176

Alternate gene names: 148651992

Gene position: 211558-213015 (Clockwise)

Preceding gene: 148651987

Following gene: 148651993

Centisome position: 7.1

GC content: 50.0

Gene sequence:

>1458_bases
GTGTCGCATTACACCCCTGCGCATACAGATCAAACTGAGCCACAAAGTGGTGGCCTGATTCAAAAAGGCGAGGGGCTACA
GGTGGTAATCGGTCTGGGTCAATCAGGTTTGGCTACAGCCAACTATCTGGCCCGCCGCGGCTATAAGGTGGCGGTAACTG
ATGGCAATAGCGCCCCTAAGCTTGCTGAGCAGTTAGAGCCGAATATCAGCATTCGTGCATTTGGTGACATTGATGCAGAA
CTGTTGTTACAAGCCTCACGTATCATTATTAGCCCAGGGGTATCGCTGGCGACACCGGCTGTGGCGGCGGCTAAACAGGC
TGGCATTGCCATTGTGAGTGATATTCAGCTGTTTAAAGAAGCGTGCAAGGCGCCGATTGTTGCCATTACTGGGTCTAATG
CCAAAAGTACCGTCACCACTTTGGTGGGTCAAATGGCCAAAGATGCGGGCGTGAATGTGGGTGTTGGTGGCAATATTGGG
GTACCGGCGCTGAGTCTCTTAGAAGATGAGGCGCTTGGTGAGGCCATGCAGCTTGCGGTATTAGAGTTGTCGAGCTTTCA
GCTAGAGACAGTGACCAATCTTGGCGCAAAAGTGGCCACTGTGCTTAATATGTCGCCGGATCACTTAGACCGTCATGGCG
ATATGCTCGGCTACCATCAAGCCAAGCACCGTATCTTCCAAGGGGCACAGTCGGTCGTGGTTAACCGTGATGATGCCTTG
TCTCGCCCTTTAATTGCGGATAATACGCCGCGTCTAAGTGTCGGATCTGGCGCCCCTGATTTGGGCGAATATGGTCTAAT
TACAGAAGGCGATGGGCGCATTTATTTGGCCAAAGGCACTGAGAAGTTATTGGCAGCAGATCAGCTCAAAGTTAAAGGCA
AGCATAATCTGGTTAATGCGTTAGCCGCCTTGGCGCTTGGGGGGCTTGCTGGCTTGCCAAAACAGAGCATGTTAAAAACG
TTACAAGAGTTTTCTGGACTGCCACACCGCTGTCAATTTGTGGATAATGTGGCGGGCGTGGATTATTTCAATGATTCTAA
AGGCACCAATGTCGGCTCAACTTTGGCGGCCATTTTGGGTTTAGGCTCAGTGTATGGCAATCAAAGTGGCGCTAAGACCA
AGTTGATGCTGATTTTAGGCGGGCAGGGCAAAGGTCAGCAGTTTGGCGAGCTTGTGCCTTTAATTAATAAATACGTCAGC
CAAATCCTACTGATAGGACAAGATGCCGAGCTAATAGAGTCGCATTTGCAGCAGGCCAATTTAAGTGGTGATGTCGAGAC
TCATCAGTGCCACGACTTGGCTCAGGCGATGACGAAAGCAAACAGCTTATTATCGAGCAGCCTATCACAAGTAACTACCG
TTTTATTATCGCCTGCTTGTGCCAGCTTAGATCAGTATCCTAGCTATGCTAAGCGTGGTGAGCACTTTTGTGAGCTGGTA
GGTAAGCTCAAAGATTAG

Upstream 100 bases:

>100_bases
TACTGACCCAACCGTTACCCCATCCCAGAACCAACAAAGCACAGACTATGTGTCACATACCCGCACGCCTCATCGAAGCC
ATAGTAGCGAAAAAGATGCG

Downstream 100 bases:

>100_bases
TTTGACCATTTAATAATTGCCTAACATATCGCGCTTTATCACGTGTTAGGTACTTATAAAAAGATAGAGGCTTGGCCTTT
ATCCTCATTTAACATACAGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSHYTPAHTDQTEPQSGGLIQKGEGLQVVIGLGQSGLATANYLARRGYKVAVTDGNSAPKLAEQLEPNISIRAFGDIDAE
LLLQASRIIISPGVSLATPAVAAAKQAGIAIVSDIQLFKEACKAPIVAITGSNAKSTVTTLVGQMAKDAGVNVGVGGNIG
VPALSLLEDEALGEAMQLAVLELSSFQLETVTNLGAKVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAQSVVVNRDDAL
SRPLIADNTPRLSVGSGAPDLGEYGLITEGDGRIYLAKGTEKLLAADQLKVKGKHNLVNALAALALGGLAGLPKQSMLKT
LQEFSGLPHRCQFVDNVAGVDYFNDSKGTNVGSTLAAILGLGSVYGNQSGAKTKLMLILGGQGKGQQFGELVPLINKYVS
QILLIGQDAELIESHLQQANLSGDVETHQCHDLAQAMTKANSLLSSSLSQVTTVLLSPACASLDQYPSYAKRGEHFCELV
GKLKD

Sequences:

>Translated_485_residues
MSHYTPAHTDQTEPQSGGLIQKGEGLQVVIGLGQSGLATANYLARRGYKVAVTDGNSAPKLAEQLEPNISIRAFGDIDAE
LLLQASRIIISPGVSLATPAVAAAKQAGIAIVSDIQLFKEACKAPIVAITGSNAKSTVTTLVGQMAKDAGVNVGVGGNIG
VPALSLLEDEALGEAMQLAVLELSSFQLETVTNLGAKVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAQSVVVNRDDAL
SRPLIADNTPRLSVGSGAPDLGEYGLITEGDGRIYLAKGTEKLLAADQLKVKGKHNLVNALAALALGGLAGLPKQSMLKT
LQEFSGLPHRCQFVDNVAGVDYFNDSKGTNVGSTLAAILGLGSVYGNQSGAKTKLMLILGGQGKGQQFGELVPLINKYVS
QILLIGQDAELIESHLQQANLSGDVETHQCHDLAQAMTKANSLLSSSLSQVTTVLLSPACASLDQYPSYAKRGEHFCELV
GKLKD
>Mature_484_residues
SHYTPAHTDQTEPQSGGLIQKGEGLQVVIGLGQSGLATANYLARRGYKVAVTDGNSAPKLAEQLEPNISIRAFGDIDAEL
LLQASRIIISPGVSLATPAVAAAKQAGIAIVSDIQLFKEACKAPIVAITGSNAKSTVTTLVGQMAKDAGVNVGVGGNIGV
PALSLLEDEALGEAMQLAVLELSSFQLETVTNLGAKVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAQSVVVNRDDALS
RPLIADNTPRLSVGSGAPDLGEYGLITEGDGRIYLAKGTEKLLAADQLKVKGKHNLVNALAALALGGLAGLPKQSMLKTL
QEFSGLPHRCQFVDNVAGVDYFNDSKGTNVGSTLAAILGLGSVYGNQSGAKTKLMLILGGQGKGQQFGELVPLINKYVSQ
ILLIGQDAELIESHLQQANLSGDVETHQCHDLAQAMTKANSLLSSSLSQVTTVLLSPACASLDQYPSYAKRGEHFCELVG
KLKD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=45.7330415754923, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50728; Mature: 50596

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYTPAHTDQTEPQSGGLIQKGEGLQVVIGLGQSGLATANYLARRGYKVAVTDGNSAPK
CCCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCHH
LAEQLEPNISIRAFGDIDAELLLQASRIIISPGVSLATPAVAAAKQAGIAIVSDIQLFKE
HHHHCCCCCEEEEECCCCHHHHHHHHHEEECCCCCHHCHHHHHHHHCCCHHHHHHHHHHH
ACKAPIVAITGSNAKSTVTTLVGQMAKDAGVNVGVGGNIGVPALSLLEDEALGEAMQLAV
HHCCCEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHH
LELSSFQLETVTNLGAKVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAQSVVVNRDDAL
HHHHCHHHHHHHHCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCHHH
SRPLIADNTPRLSVGSGAPDLGEYGLITEGDGRIYLAKGTEKLLAADQLKVKGKHNLVNA
CCCCCCCCCCCEEECCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHCCCHHHHHHH
LAALALGGLAGLPKQSMLKTLQEFSGLPHRCQFVDNVAGVDYFNDSKGTNVGSTLAAILG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
LGSVYGNQSGAKTKLMLILGGQGKGQQFGELVPLINKYVSQILLIGQDAELIESHLQQAN
HHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCC
LSGDVETHQCHDLAQAMTKANSLLSSSLSQVTTVLLSPACASLDQYPSYAKRGEHFCELV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GKLKD
HHCCC
>Mature Secondary Structure 
SHYTPAHTDQTEPQSGGLIQKGEGLQVVIGLGQSGLATANYLARRGYKVAVTDGNSAPK
CCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCHH
LAEQLEPNISIRAFGDIDAELLLQASRIIISPGVSLATPAVAAAKQAGIAIVSDIQLFKE
HHHHCCCCCEEEEECCCCHHHHHHHHHEEECCCCCHHCHHHHHHHHCCCHHHHHHHHHHH
ACKAPIVAITGSNAKSTVTTLVGQMAKDAGVNVGVGGNIGVPALSLLEDEALGEAMQLAV
HHCCCEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHH
LELSSFQLETVTNLGAKVATVLNMSPDHLDRHGDMLGYHQAKHRIFQGAQSVVVNRDDAL
HHHHCHHHHHHHHCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCHHH
SRPLIADNTPRLSVGSGAPDLGEYGLITEGDGRIYLAKGTEKLLAADQLKVKGKHNLVNA
CCCCCCCCCCCEEECCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHCCCHHHHHHH
LAALALGGLAGLPKQSMLKTLQEFSGLPHRCQFVDNVAGVDYFNDSKGTNVGSTLAAILG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
LGSVYGNQSGAKTKLMLILGGQGKGQQFGELVPLINKYVSQILLIGQDAELIESHLQQAN
HHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCC
LSGDVETHQCHDLAQAMTKANSLLSSSLSQVTTVLLSPACASLDQYPSYAKRGEHFCELV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GKLKD
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA