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The map label for this gene is pnp

Identifier: 148651980

GI number: 148651980

Start: 193089

End: 195203

Strand: Direct

Name: pnp

Synonym: PsycPRwf_0164

Alternate gene names: 148651980

Gene position: 193089-195203 (Clockwise)

Preceding gene: 148651979

Following gene: 148651982

Centisome position: 6.48

GC content: 46.24

Gene sequence:

>2115_bases
ATGTCAATGTTTAATACCATCTCTCGAGAGTTCCAGTACGGCAACGAACAAGTTGTTATTGAAACCGGTCGTATCGCACG
ACAAGCCAATTCAGTATTGGTTCATATGGGCGGCGTATCAGTATTGGTCGCTGTGGTTGTAAAAAGCGATGCTAAAGAAG
GTCAAAACTTCTTCCCGCTAACCGTCAACTATCAAGAAAAAATGTACGCTGCTGGTAAAATTCCAGGCGCTTACGGCAAA
CGTGAAGGCCGTCCTACTGAGTTTGAAACCCTAACCTCACGTCTCATTGACCGTCCCATCCGTCCTTTATTCCCAGAAGG
TTATGTCAACGAGATCCAGATTACTGCCACAGTCATCTCAAGTGACAAAAAGCACTATGCTGATATCGCTGCCATGATTG
GTGCTTCAGCTGCACTTTCAATCTCAGATGCGCCGTTTAATGGCCCTATTGGTGGTGCCCGCGTAGGCTTTATCAATGGC
GAATACGTGCTTAACCCCTCAATTGAAGAGTTAAAGAGCAGTGATCTTGACTTAGTAGTGGCGGGTACTAAATCAGCGGT
ATTAATGGTTGAGTCAGAAGCTGCTGAGCTGTCTGAAGACCAAATGCTGGGCGCTGTATTGTACGGTCATGAACAGCAGC
AAATTGTTATCGATAATATTGCTGAGCTGGCCAAGGCCGTGGGAACTCAAAAACAAGACTTTGTGGCACCTGAGCTAAAT
GCTGAGTTAAAAGAAAAAGTAACCACACAGTTTGGTGAGCAAGTGGCTGAAGCTTATGCCATTAGCGATAAGCAAGCACG
CTACGAAAAACTAGATGAGATCAAGGCTGCGGCTATTGACGCCTTAGCAGGTGACCCTGAGTCAGAAGAGTTTGCTGAAA
AAGAAGCTCAAATTAAAGAAATCTATAATGATCTAAAATATCGCACCGTTCGTGACGCCATTTTGTCAGGTAAGCCACGT
ATCGATGGTCGTGATTTAGATACCGTTCGTGCGCTAGATATTCAAGTAGGTGTATTGCCTTACACTCATGGTTCAGCGCT
ATTTACCCGTGGTGAGACGCAAGCTCTAGTCACCACGACTTTAGGTAATTCACGTGATGTAAACTTGATTGACTCGCTAG
CGGGTACTATCCAAGATCATTTCATGCTGCATTACAACTTCCCGCATTATTCAGTGGGTGAAACTGGCCGTGAAGGCGTG
CCAAAGCGTCGTGAAATTGGTCATGGCCGTCTGGCTCGCCGTGGTGTTCAAGCCATGCTTCCAGACAGCGACCGCTTCCC
ATACGTTATCCGTGTGGTGTCTGAGATTACTGAATCAAATGGTTCGTCTTCAATGGCGTCAGTTTGTGGTGCCAGCCTTG
CCTTAATGGATGCCGGTGTGCCGTTAAAAGCCCCAGTAGCTGGTATCGCTATGGGTCTGGTCAAAGAAGGCGACCGCTTT
GCTGTCTTATCAGATATCCTAGGTGATGAAGATCATTTAGGTGATATGGACTTTAAAGTGGCCGGTACTAAAGATGGTAT
CACAGCCTTACAGATGGATATTAAGATTGAAGGTATCACCTCAGATATCATGGAAAAAGCACTTGAGCAAGCTCATGCCG
GCCGTATCCATATCTTAAATGCCATGAACGAAGTATTGCCAGCCAGCCGTACTGAAATCAATGCCCATGCACCAAACTAT
GCGGTCATCGAAATCAATTCTGATAAAATCCGTGATGTGATCGGTAAAGGCGGCGCAACCATCCGTCAATTAACCGAAGA
CACCGGTGCGGTTATCGATATTGATGACAATGGTACCATTCGTATCTTTGGTGAAAACAAAGCCGCGACCAAAGAAGCCA
TCCGTCAAATCGAAGCCATCACCGCTGAAGTGGAAGTGGGCAAAGTATACAAAGGCACCGTTGCGCGCGTTGTAGACTTT
GGTGCATTCGTAACGGTTTTACCAGGTACTGATGGTCTGGTACACATCTCACAAATCGCTGATGAGCGTGTAGAGAGCGT
ATCGGATTACCTAAGTGAAGGCCAACAAATCAATGTGTTGGTGCAAGATGTAGACAACCGTGGTCGCATCAAATTAACCA
TGAAAGGCGTTGAGCAAGAGCAGACTCAAGCTTAA

Upstream 100 bases:

>100_bases
TATTTGGATAAGAGCAGTTAAACGTTAGAGGCTAACACTAAGTCGCAATAGACGAATTTATTCATTCGTATCCGAAGTTT
ATAAAAAATAGGAAAAATTA

Downstream 100 bases:

>100_bases
GTCTATTCAAGGTTAGGCGTAAAAAACATTGCGCCTATCAAAGTAGATCCCATCCAAAAATAAGTGGATGAATTAAAAAA
ACCAGATACTCGTGTATCTG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MSMFNTISREFQYGNEQVVIETGRIARQANSVLVHMGGVSVLVAVVVKSDAKEGQNFFPLTVNYQEKMYAAGKIPGAYGK
REGRPTEFETLTSRLIDRPIRPLFPEGYVNEIQITATVISSDKKHYADIAAMIGASAALSISDAPFNGPIGGARVGFING
EYVLNPSIEELKSSDLDLVVAGTKSAVLMVESEAAELSEDQMLGAVLYGHEQQQIVIDNIAELAKAVGTQKQDFVAPELN
AELKEKVTTQFGEQVAEAYAISDKQARYEKLDEIKAAAIDALAGDPESEEFAEKEAQIKEIYNDLKYRTVRDAILSGKPR
IDGRDLDTVRALDIQVGVLPYTHGSALFTRGETQALVTTTLGNSRDVNLIDSLAGTIQDHFMLHYNFPHYSVGETGREGV
PKRREIGHGRLARRGVQAMLPDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGDRF
AVLSDILGDEDHLGDMDFKVAGTKDGITALQMDIKIEGITSDIMEKALEQAHAGRIHILNAMNEVLPASRTEINAHAPNY
AVIEINSDKIRDVIGKGGATIRQLTEDTGAVIDIDDNGTIRIFGENKAATKEAIRQIEAITAEVEVGKVYKGTVARVVDF
GAFVTVLPGTDGLVHISQIADERVESVSDYLSEGQQINVLVQDVDNRGRIKLTMKGVEQEQTQA

Sequences:

>Translated_704_residues
MSMFNTISREFQYGNEQVVIETGRIARQANSVLVHMGGVSVLVAVVVKSDAKEGQNFFPLTVNYQEKMYAAGKIPGAYGK
REGRPTEFETLTSRLIDRPIRPLFPEGYVNEIQITATVISSDKKHYADIAAMIGASAALSISDAPFNGPIGGARVGFING
EYVLNPSIEELKSSDLDLVVAGTKSAVLMVESEAAELSEDQMLGAVLYGHEQQQIVIDNIAELAKAVGTQKQDFVAPELN
AELKEKVTTQFGEQVAEAYAISDKQARYEKLDEIKAAAIDALAGDPESEEFAEKEAQIKEIYNDLKYRTVRDAILSGKPR
IDGRDLDTVRALDIQVGVLPYTHGSALFTRGETQALVTTTLGNSRDVNLIDSLAGTIQDHFMLHYNFPHYSVGETGREGV
PKRREIGHGRLARRGVQAMLPDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGDRF
AVLSDILGDEDHLGDMDFKVAGTKDGITALQMDIKIEGITSDIMEKALEQAHAGRIHILNAMNEVLPASRTEINAHAPNY
AVIEINSDKIRDVIGKGGATIRQLTEDTGAVIDIDDNGTIRIFGENKAATKEAIRQIEAITAEVEVGKVYKGTVARVVDF
GAFVTVLPGTDGLVHISQIADERVESVSDYLSEGQQINVLVQDVDNRGRIKLTMKGVEQEQTQA
>Mature_703_residues
SMFNTISREFQYGNEQVVIETGRIARQANSVLVHMGGVSVLVAVVVKSDAKEGQNFFPLTVNYQEKMYAAGKIPGAYGKR
EGRPTEFETLTSRLIDRPIRPLFPEGYVNEIQITATVISSDKKHYADIAAMIGASAALSISDAPFNGPIGGARVGFINGE
YVLNPSIEELKSSDLDLVVAGTKSAVLMVESEAAELSEDQMLGAVLYGHEQQQIVIDNIAELAKAVGTQKQDFVAPELNA
ELKEKVTTQFGEQVAEAYAISDKQARYEKLDEIKAAAIDALAGDPESEEFAEKEAQIKEIYNDLKYRTVRDAILSGKPRI
DGRDLDTVRALDIQVGVLPYTHGSALFTRGETQALVTTTLGNSRDVNLIDSLAGTIQDHFMLHYNFPHYSVGETGREGVP
KRREIGHGRLARRGVQAMLPDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGDRFA
VLSDILGDEDHLGDMDFKVAGTKDGITALQMDIKIEGITSDIMEKALEQAHAGRIHILNAMNEVLPASRTEINAHAPNYA
VIEINSDKIRDVIGKGGATIRQLTEDTGAVIDIDDNGTIRIFGENKAATKEAIRQIEAITAEVEVGKVYKGTVARVVDFG
AFVTVLPGTDGLVHISQIADERVESVSDYLSEGQQINVLVQDVDNRGRIKLTMKGVEQEQTQA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=37.4301675977654, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI145693187, Length=701, Percent_Identity=61.7689015691869, Blast_Score=869, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=666, Percent_Identity=33.033033033033, Blast_Score=319, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17535281, Length=78, Percent_Identity=46.1538461538462, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=105, Percent_Identity=35.2380952380952, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281362905, Length=699, Percent_Identity=37.6251788268956, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=699, Percent_Identity=37.6251788268956, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=699, Percent_Identity=37.6251788268956, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=37.3831775700935, Blast_Score=407, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PSYWF (A5WBT2)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279073.1
- ProteinModelPortal:   A5WBT2
- SMR:   A5WBT2
- STRING:   A5WBT2
- GeneID:   5205685
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0164
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   PSP56811:PSYCPRWF_0164-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76266; Mature: 76135

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFNTISREFQYGNEQVVIETGRIARQANSVLVHMGGVSVLVAVVVKSDAKEGQNFFPL
CCHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECHHHHHHHHHHHCCHHCCCCEEEE
TVNYQEKMYAAGKIPGAYGKREGRPTEFETLTSRLIDRPIRPLFPEGYVNEIQITATVIS
EEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEC
SDKKHYADIAAMIGASAALSISDAPFNGPIGGARVGFINGEYVLNPSIEELKSSDLDLVV
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCEEECCCHHHHCCCCCEEEE
AGTKSAVLMVESEAAELSEDQMLGAVLYGHEQQQIVIDNIAELAKAVGTQKQDFVAPELN
ECCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHCCCCCCC
AELKEKVTTQFGEQVAEAYAISDKQARYEKLDEIKAAAIDALAGDPESEEFAEKEAQIKE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IYNDLKYRTVRDAILSGKPRIDGRDLDTVRALDIQVGVLPYTHGSALFTRGETQALVTTT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEECCCEEEECCCCCEEEEEE
LGNSRDVNLIDSLAGTIQDHFMLHYNFPHYSVGETGREGVPKRREIGHGRLARRGVQAML
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHCHHHCC
PDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGDRF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCE
AVLSDILGDEDHLGDMDFKVAGTKDGITALQMDIKIEGITSDIMEKALEQAHAGRIHILN
EEHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCEEEEEH
AMNEVLPASRTEINAHAPNYAVIEINSDKIRDVIGKGGATIRQLTEDTGAVIDIDDNGTI
HHHHHCCCCCCEEECCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEE
RIFGENKAATKEAIRQIEAITAEVEVGKVYKGTVARVVDFGAFVTVLPGTDGLVHISQIA
EEEECCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHH
DERVESVSDYLSEGQQINVLVQDVDNRGRIKLTMKGVEQEQTQA
HHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCHHHCCC
>Mature Secondary Structure 
SMFNTISREFQYGNEQVVIETGRIARQANSVLVHMGGVSVLVAVVVKSDAKEGQNFFPL
CHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECHHHHHHHHHHHCCHHCCCCEEEE
TVNYQEKMYAAGKIPGAYGKREGRPTEFETLTSRLIDRPIRPLFPEGYVNEIQITATVIS
EEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEC
SDKKHYADIAAMIGASAALSISDAPFNGPIGGARVGFINGEYVLNPSIEELKSSDLDLVV
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCEEECCCHHHHCCCCCEEEE
AGTKSAVLMVESEAAELSEDQMLGAVLYGHEQQQIVIDNIAELAKAVGTQKQDFVAPELN
ECCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHCCCCCCC
AELKEKVTTQFGEQVAEAYAISDKQARYEKLDEIKAAAIDALAGDPESEEFAEKEAQIKE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IYNDLKYRTVRDAILSGKPRIDGRDLDTVRALDIQVGVLPYTHGSALFTRGETQALVTTT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEECCCEEEECCCCCEEEEEE
LGNSRDVNLIDSLAGTIQDHFMLHYNFPHYSVGETGREGVPKRREIGHGRLARRGVQAML
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHCHHHCC
PDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGDRF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCE
AVLSDILGDEDHLGDMDFKVAGTKDGITALQMDIKIEGITSDIMEKALEQAHAGRIHILN
EEHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCEEEEEH
AMNEVLPASRTEINAHAPNYAVIEINSDKIRDVIGKGGATIRQLTEDTGAVIDIDDNGTI
HHHHHCCCCCCEEECCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEE
RIFGENKAATKEAIRQIEAITAEVEVGKVYKGTVARVVDFGAFVTVLPGTDGLVHISQIA
EEEECCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHH
DERVESVSDYLSEGQQINVLVQDVDNRGRIKLTMKGVEQEQTQA
HHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA