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The map label for this gene is oppA [H]

Identifier: 148651838

GI number: 148651838

Start: 31051

End: 32736

Strand: Direct

Name: oppA [H]

Synonym: PsycPRwf_0021

Alternate gene names: 148651838

Gene position: 31051-32736 (Clockwise)

Preceding gene: 148651837

Following gene: 148651839

Centisome position: 1.04

GC content: 49.05

Gene sequence:

>1686_bases
ATGAAGATTTTAAGTCGTACAACATTACCGACACGCTTGCCACTGGTGATGGCTGTCCTAGCTGGCCTATCACTGAGTGC
GTGTGGTGACAAAAACCCACAAGAGAGCGGTAGCACAGCCAAGACCTCTGAGCAGATTGAGCAGACAGCCTTGGCCGATA
CGCAGCACATCGTCATTAATAACACTGCCGAACCTGAGTCGATAGACCCGCACAAAGTCTCAGCGGTGCCAGAAGGCAAC
ATCATCCGTCAGATGTTCACTGGGTTGGTCGATACAGACCGAGAAGGCAAAACTGTGCCTGGTATGGCTGAAAGCTGGGA
GAGTAGTGACAATAAGGTCTGGACCTTTAAGCTGCGTGATGCCAAGTGGAGTAATGGCGATCCGGTAACCGCTGGCGACT
TCGTTTACAGTTGGCAGCGTCTCATTGACCCAAATACCGCTGCACCTTATGCCAGCTACTTGGTCGATGCCAAAGTGGCG
GGCGCTGAAGCCATCATGGAGGGCAAAGCTGCAGTAGATACCTTGGGCATTAAAGCGCTTGATGACAAGACGCTACAAGT
TACCTTAACCGAAGCTGTGCCTTACTTTCCTGACATGCTTATTCACACCTCTACCAAGCCGGTGCCTCAAAAGGTCATTG
CTGAGTTTGGGGAGAAGTGGACGGCACCTGAGCACATCGTGGTGAATGGCCCTTATACGTTAAGTGAGTGGTCAGTGAAT
GACAAAATTGTGCTGACCCGCAATACCCATTACTTTGATAATGCGAACACCAAAATTGATCAAGTCACCATTTTACCGCT
GTCATCGCCCACAACAGATGTGGCACGTTACCGAGCAGGCGAGGTGGATGTCAGTGCTGAAGTACCGCCTGAGCAGTTTG
ATATGCTCAAAAAAGAGCTGGGCGATGAGCTCAGAGTAGCACCTAGATTGTGTACTTATTACTACAAATACAACACCGTC
AAGCCCCCATTTAATGATGCGCGCGTACGCCGAGCTTTGGCCTTGGCTTTAGATCGTGACACCATTGCCACTAAGATTAT
GGGGCAAGGTCAAGCGCCTGCTTATCAGTTGACGCCGCCGATTACCAATGGCATGGAGAACTTCACTCCGCAGTGGGAGA
GCTGGAGCAAACAGCAGCGCATTGATGAGGCAAAAAAACTATTAAACGAGGCAGGCTACAATGCCAGCCATCCACTGAAG
TTTGAGCTGTTGTATAACACCAGTGACAACCATAAAAAAATTGCCGTGGCCTTTAGCGCTTTGATGAAGCAAGCGCTAGG
GGAGGACACAGTGGATGTTACCTTGGTCAATCAAGAGTGGAAAACCTACTTAGATACCCGCCGATTGGGCAACTATGACA
TTGCCCGCTCAAGCTGGTGTGGGGACTATAATGAACCATCCTCGTTTTTAAACACACTTAAGACTGACAACACCAATAAC
CACGGTAAATACTCCAGTGCTAAGTTTGACAGCATTATGAATCAAACCTTGGTTGAAGGCGTCGATGCGACGCTGCGTGC
CAAGCTATATCAACAGGCTGAAGCACAGTTAGATGCGGATATGCCCAATCTAAACATGTTCCATTATGTTGGGGTACGTC
TGGTTAAACCTTATGTGGTGGGCTTCCCAGAAAACGATCCACTAGATGTGTGGCAAGCCAAAGACTTGGCCATTGCTAAG
CATTAA

Upstream 100 bases:

>100_bases
TGAGCAATTGGCAGATTAAGGATGTCTCTATCGCCAAGCATGACTAAAAAGAGTGGCTGCGATGAGGGTTGGATTTGGTA
TTAATAAAAGTGATTAAATT

Downstream 100 bases:

>100_bases
GGGTAAAGCACACCCATAGCAGATAAAGAATAAGCACCAAACACGGATATTAATTATCGATTTTAACAACGGAATCAATG
CGTTACTGATAACAGAGGGT

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKILSRTTLPTRLPLVMAVLAGLSLSACGDKNPQESGSTAKTSEQIEQTALADTQHIVINNTAEPESIDPHKVSAVPEGN
IIRQMFTGLVDTDREGKTVPGMAESWESSDNKVWTFKLRDAKWSNGDPVTAGDFVYSWQRLIDPNTAAPYASYLVDAKVA
GAEAIMEGKAAVDTLGIKALDDKTLQVTLTEAVPYFPDMLIHTSTKPVPQKVIAEFGEKWTAPEHIVVNGPYTLSEWSVN
DKIVLTRNTHYFDNANTKIDQVTILPLSSPTTDVARYRAGEVDVSAEVPPEQFDMLKKELGDELRVAPRLCTYYYKYNTV
KPPFNDARVRRALALALDRDTIATKIMGQGQAPAYQLTPPITNGMENFTPQWESWSKQQRIDEAKKLLNEAGYNASHPLK
FELLYNTSDNHKKIAVAFSALMKQALGEDTVDVTLVNQEWKTYLDTRRLGNYDIARSSWCGDYNEPSSFLNTLKTDNTNN
HGKYSSAKFDSIMNQTLVEGVDATLRAKLYQQAEAQLDADMPNLNMFHYVGVRLVKPYVVGFPENDPLDVWQAKDLAIAK
H

Sequences:

>Translated_561_residues
MKILSRTTLPTRLPLVMAVLAGLSLSACGDKNPQESGSTAKTSEQIEQTALADTQHIVINNTAEPESIDPHKVSAVPEGN
IIRQMFTGLVDTDREGKTVPGMAESWESSDNKVWTFKLRDAKWSNGDPVTAGDFVYSWQRLIDPNTAAPYASYLVDAKVA
GAEAIMEGKAAVDTLGIKALDDKTLQVTLTEAVPYFPDMLIHTSTKPVPQKVIAEFGEKWTAPEHIVVNGPYTLSEWSVN
DKIVLTRNTHYFDNANTKIDQVTILPLSSPTTDVARYRAGEVDVSAEVPPEQFDMLKKELGDELRVAPRLCTYYYKYNTV
KPPFNDARVRRALALALDRDTIATKIMGQGQAPAYQLTPPITNGMENFTPQWESWSKQQRIDEAKKLLNEAGYNASHPLK
FELLYNTSDNHKKIAVAFSALMKQALGEDTVDVTLVNQEWKTYLDTRRLGNYDIARSSWCGDYNEPSSFLNTLKTDNTNN
HGKYSSAKFDSIMNQTLVEGVDATLRAKLYQQAEAQLDADMPNLNMFHYVGVRLVKPYVVGFPENDPLDVWQAKDLAIAK
H
>Mature_561_residues
MKILSRTTLPTRLPLVMAVLAGLSLSACGDKNPQESGSTAKTSEQIEQTALADTQHIVINNTAEPESIDPHKVSAVPEGN
IIRQMFTGLVDTDREGKTVPGMAESWESSDNKVWTFKLRDAKWSNGDPVTAGDFVYSWQRLIDPNTAAPYASYLVDAKVA
GAEAIMEGKAAVDTLGIKALDDKTLQVTLTEAVPYFPDMLIHTSTKPVPQKVIAEFGEKWTAPEHIVVNGPYTLSEWSVN
DKIVLTRNTHYFDNANTKIDQVTILPLSSPTTDVARYRAGEVDVSAEVPPEQFDMLKKELGDELRVAPRLCTYYYKYNTV
KPPFNDARVRRALALALDRDTIATKIMGQGQAPAYQLTPPITNGMENFTPQWESWSKQQRIDEAKKLLNEAGYNASHPLK
FELLYNTSDNHKKIAVAFSALMKQALGEDTVDVTLVNQEWKTYLDTRRLGNYDIARSSWCGDYNEPSSFLNTLKTDNTNN
HGKYSSAKFDSIMNQTLVEGVDATLRAKLYQQAEAQLDADMPNLNMFHYVGVRLVKPYVVGFPENDPLDVWQAKDLAIAK
H

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=547, Percent_Identity=52.8336380255942, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI87081878, Length=530, Percent_Identity=42.2641509433962, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789397, Length=515, Percent_Identity=45.6310679611651, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1787762, Length=488, Percent_Identity=26.2295081967213, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789966, Length=534, Percent_Identity=24.3445692883895, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787052, Length=347, Percent_Identity=25.6484149855908, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789887, Length=489, Percent_Identity=24.5398773006135, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787551, Length=470, Percent_Identity=24.468085106383, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62503; Mature: 62503

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILSRTTLPTRLPLVMAVLAGLSLSACGDKNPQESGSTAKTSEQIEQTALADTQHIVIN
CCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
NTAEPESIDPHKVSAVPEGNIIRQMFTGLVDTDREGKTVPGMAESWESSDNKVWTFKLRD
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEEEEC
AKWSNGDPVTAGDFVYSWQRLIDPNTAAPYASYLVDAKVAGAEAIMEGKAAVDTLGIKAL
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHCEEEE
DDKTLQVTLTEAVPYFPDMLIHTSTKPVPQKVIAEFGEKWTAPEHIVVNGPYTLSEWSVN
CCCEEEEEEHHCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCEEECCCCCC
DKIVLTRNTHYFDNANTKIDQVTILPLSSPTTDVARYRAGEVDVSAEVPPEQFDMLKKEL
CEEEEEECCEEECCCCCEEEEEEEEECCCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHC
GDELRVAPRLCTYYYKYNTVKPPFNDARVRRALALALDRDTIATKIMGQGQAPAYQLTPP
CCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCC
ITNGMENFTPQWESWSKQQRIDEAKKLLNEAGYNASHPLKFELLYNTSDNHKKIAVAFSA
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
LMKQALGEDTVDVTLVNQEWKTYLDTRRLGNYDIARSSWCGDYNEPSSFLNTLKTDNTNN
HHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
HGKYSSAKFDSIMNQTLVEGVDATLRAKLYQQAEAQLDADMPNLNMFHYVGVRLVKPYVV
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEECCEEE
GFPENDPLDVWQAKDLAIAKH
ECCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MKILSRTTLPTRLPLVMAVLAGLSLSACGDKNPQESGSTAKTSEQIEQTALADTQHIVIN
CCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
NTAEPESIDPHKVSAVPEGNIIRQMFTGLVDTDREGKTVPGMAESWESSDNKVWTFKLRD
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEEEEC
AKWSNGDPVTAGDFVYSWQRLIDPNTAAPYASYLVDAKVAGAEAIMEGKAAVDTLGIKAL
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHCEEEE
DDKTLQVTLTEAVPYFPDMLIHTSTKPVPQKVIAEFGEKWTAPEHIVVNGPYTLSEWSVN
CCCEEEEEEHHCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCEEECCCCCC
DKIVLTRNTHYFDNANTKIDQVTILPLSSPTTDVARYRAGEVDVSAEVPPEQFDMLKKEL
CEEEEEECCEEECCCCCEEEEEEEEECCCCCHHHHHHCCCCEEEECCCCHHHHHHHHHHC
GDELRVAPRLCTYYYKYNTVKPPFNDARVRRALALALDRDTIATKIMGQGQAPAYQLTPP
CCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCC
ITNGMENFTPQWESWSKQQRIDEAKKLLNEAGYNASHPLKFELLYNTSDNHKKIAVAFSA
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
LMKQALGEDTVDVTLVNQEWKTYLDTRRLGNYDIARSSWCGDYNEPSSFLNTLKTDNTNN
HHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
HGKYSSAKFDSIMNQTLVEGVDATLRAKLYQQAEAQLDADMPNLNMFHYVGVRLVKPYVV
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEECCEEE
GFPENDPLDVWQAKDLAIAKH
ECCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]