| Definition | Psychrobacter sp. PRwf-1 chromosome, complete genome. |
|---|---|
| Accession | NC_009524 |
| Length | 2,978,976 |
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The map label for this gene is gyrB [H]
Identifier: 148651822
GI number: 148651822
Start: 6078
End: 8732
Strand: Direct
Name: gyrB [H]
Synonym: PsycPRwf_0005
Alternate gene names: 148651822
Gene position: 6078-8732 (Clockwise)
Preceding gene: 148651821
Following gene: 148651823
Centisome position: 0.2
GC content: 46.4
Gene sequence:
>2655_bases ATGAGTGAATCAAACCATAATACCGAGCAAGCGCTAGTACAAGGCTTGCCTGAAGGGGTCGATGCCACAGACTTACCAGC AGGTGCAGAGCCAGAAGAATATAGCTCAAAAAATATTCGAGTATTAAGAGGCCTTGAAGCTGTACGCGTGCGTCCGGGAA TGTATATCGGGGATACTGATGACGGTACTGGCTTACACCACATGGTGTTTGAAGTAGTCGACAACTCAATTGACGAGGCA TTGGCAGGGCACTGTGACCAAATCGATATCATCATCCATGAAGATGAATCGGTCAGTGTGATGGACAATGGCCGTGGTAT TCCTATCGATGTGCATCCTGAAGAGGGCGTGTCGGCCGCTCAGGTCATTATGACCGTGCTGCATGCCGGTGGTAAGTTCG ATGACAACAGTTATAAGGTGTCAGGCGGCTTGCACGGCGTGGGTGTCTCAGTAGTGAACGCCTTATCCAAAAAGCTTGAG ATGAATATTTGGCGTGAAGGCCATCATTATCAGCAGACGTATAGCGATGGCGTACCAGATAGCGATATTCAAGAATTAGA AAAAACCAATAAGACAGGTACTCAAATTCGTTTTTATCCGAGCCCAGAGGTATTTACCGGTACCGTATTTGACTTTGATA TTTTAGCCAAGCGTTTACGTGAGTTGTCATTTTTAAACTCAGGGGTTCGCATTGTGCTAACCGATGAGCGTACAGACAAA CAGCATGTGTTCGAGCACAAAGGTGGGCTATCGGAGTTTGTGGCTTACATCAATTCTGGCAAAGAAGGCCTAAATGATGT CTTTCATTTTGTCAGTGAGCAAGAAGACGGTATCGTTGTGGAAGCGGCACTGCAGTGGACAGATACTTATAATGAAAAAG TGCTGTGTTTTACCAACAACATCCCACAAAAAGATGGCGGCACTCACTTATCAGGTTTCCGCTCAGCCTTAACCCGTTGT CTAAATAGCTATATGGACAATGAGAAACTGGTTAAAAAAGAAAAAGTGAACACCACAGGCGACGATGCGCGTGAAGGTTT AACCGCCATTGTTTCGGTCAAAGTACCTGATCCTAAATTCTCAAGTCAGACCAAAGACAAGCTGGTATCGAGCGAAGTAA AATCCGCTGTTGAATCAGCGATGCATGACAAGTTTAATGACTACTTGCTTGAGCATCCAGGTGCGGCAAAATCTATTGCC AATAAGATTATTGATGCGGCCCGTGCTCGCGATGCGGCCCGTAAAGCACGTGAATTAACGCGTCGTAAAACCACTTTAGA CATCGCTGGTCTGCCCGGTAAACTGGCTGATTGCCAAGAAAAAGACCCAGCATTGTCTGAATTATATATTGTGGAGGGTG ACTCAGCAGGTGGTTCTGCCAAGCAGGGTCGTAGCCGTAAAACTCAGGCTATTTTGCCGCTTAAAGGTAAGATCCTAAAC GTTGAGCGTGCCCGTTTTGACAAGATGTTATCCTCTGCTGAAGTCGGTACGTTAATCACCGCGCTAGGCTGTGGTATTGG CCCAGATGAGTACAATCCAGATAAAGTACGTTATCACAAAATCATCATTATGACCGATGCCGACGTGGATGGCTCACACA TTCGTACACTGCTTTTGACCTTCTTCTTCCGTCAAACACCAGAGCTTATCGAACGAGGCTATGTCTATATTGCTCAGCCG CCTTTGTACAAGATAAAAAAAGGCCGTCAAGAGCTGTATCTAAAAGATGATGAGGCGCTCAAAGCGTATCTATTATCCTC GACGATTGATGAGATGAATCTACACATTAGTGAAGATGCACCAGCCATTACCGGTCAAGCCTTAGAGACGTTATTAAACG ATTATAACCAAACACAACTGGTGAAAGCACGTTTGCAGATTCGTTATCCTGCAATATTGCTCGATGCTTTGACTCATGTG CCTAAGCTGACTACAGACATGACTTACGACTTCGATGCCATGACTGAGTGGAAGCAGCAGTTGCAGGCTCAGCTAGAGCA CTTCGGTAGTGATTTGCGCCCCTCCATTGAGCTTGATAACATCCATGCGCCGCGCACAGATGAGCTGTCTGAAGCGGCTA AAGATGCCAACAATCAGTTTAAATTTAAAATCGGCGCAAAAGTAGACAGCCCGAATAGTGATATTGAAGATGCCGAAGTT GTGGAAGGTCAAGACAGCGACATGCTATCAACCAAGCCGCAGTGGTTGCCTCGTATTACCATTTACGTACATAACTTGGC ACAGCATTATCTGCTTGATGCCGGCTTCTTTAATTCAGGCGAATACGCACGCTTGATGCGTCTGTCAAAACAGTGGAATA CGTTGCTGACAGAGTCCGCCTTTATCCGCCGTGATACCACAGCTGGTGGCAGCAAAGATACTCTAATTCGCAACTTTGAT CATCTATGGCAACAAGTGATGCAAGAAGCCCGTCGTGGTCTGTCTGTGCAGCGCTACAAAGGGCTGGGCGAGATGAACGC CGACCAGCTTTGGGACACCACCATGGATCCTGAGAACCGCCGTATGTTAAAGGTCACCATTGAGGATGCGATTGCCGCTG ATCATTTATTCATGTGCTTGATGGGCGATGATGTAGAGCCGCGCCGTCAGTTCATTGAAGAAAATGCCTTAACCGTTACC AATCTTGATATCTAA
Upstream 100 bases:
>100_bases AAACTGAAGATTAGAGAAGGTTGACAGGTCAGCTATAAAGCCACGACGCCATTTGAATTGCTGATACTCTCAGCATTGCC GTTTTAGGAAAGGATATAAC
Downstream 100 bases:
>100_bases ACGCTTAGTAGTTAAAGTCAGTAGTAGTCAAGAGAATGGACACCGTATAACGACTTAAATCACTAATAAAAAAGTCATCT TGCTAGCAAGGTGGCTTTTT
Product: DNA gyrase subunit B
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 884; Mature: 883
Protein sequence:
>884_residues MSESNHNTEQALVQGLPEGVDATDLPAGAEPEEYSSKNIRVLRGLEAVRVRPGMYIGDTDDGTGLHHMVFEVVDNSIDEA LAGHCDQIDIIIHEDESVSVMDNGRGIPIDVHPEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLE MNIWREGHHYQQTYSDGVPDSDIQELEKTNKTGTQIRFYPSPEVFTGTVFDFDILAKRLRELSFLNSGVRIVLTDERTDK QHVFEHKGGLSEFVAYINSGKEGLNDVFHFVSEQEDGIVVEAALQWTDTYNEKVLCFTNNIPQKDGGTHLSGFRSALTRC LNSYMDNEKLVKKEKVNTTGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMHDKFNDYLLEHPGAAKSIA NKIIDAARARDAARKARELTRRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN VERARFDKMLSSAEVGTLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLTFFFRQTPELIERGYVYIAQP PLYKIKKGRQELYLKDDEALKAYLLSSTIDEMNLHISEDAPAITGQALETLLNDYNQTQLVKARLQIRYPAILLDALTHV PKLTTDMTYDFDAMTEWKQQLQAQLEHFGSDLRPSIELDNIHAPRTDELSEAAKDANNQFKFKIGAKVDSPNSDIEDAEV VEGQDSDMLSTKPQWLPRITIYVHNLAQHYLLDAGFFNSGEYARLMRLSKQWNTLLTESAFIRRDTTAGGSKDTLIRNFD HLWQQVMQEARRGLSVQRYKGLGEMNADQLWDTTMDPENRRMLKVTIEDAIAADHLFMCLMGDDVEPRRQFIEENALTVT NLDI
Sequences:
>Translated_884_residues MSESNHNTEQALVQGLPEGVDATDLPAGAEPEEYSSKNIRVLRGLEAVRVRPGMYIGDTDDGTGLHHMVFEVVDNSIDEA LAGHCDQIDIIIHEDESVSVMDNGRGIPIDVHPEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLE MNIWREGHHYQQTYSDGVPDSDIQELEKTNKTGTQIRFYPSPEVFTGTVFDFDILAKRLRELSFLNSGVRIVLTDERTDK QHVFEHKGGLSEFVAYINSGKEGLNDVFHFVSEQEDGIVVEAALQWTDTYNEKVLCFTNNIPQKDGGTHLSGFRSALTRC LNSYMDNEKLVKKEKVNTTGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMHDKFNDYLLEHPGAAKSIA NKIIDAARARDAARKARELTRRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN VERARFDKMLSSAEVGTLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLTFFFRQTPELIERGYVYIAQP PLYKIKKGRQELYLKDDEALKAYLLSSTIDEMNLHISEDAPAITGQALETLLNDYNQTQLVKARLQIRYPAILLDALTHV PKLTTDMTYDFDAMTEWKQQLQAQLEHFGSDLRPSIELDNIHAPRTDELSEAAKDANNQFKFKIGAKVDSPNSDIEDAEV VEGQDSDMLSTKPQWLPRITIYVHNLAQHYLLDAGFFNSGEYARLMRLSKQWNTLLTESAFIRRDTTAGGSKDTLIRNFD HLWQQVMQEARRGLSVQRYKGLGEMNADQLWDTTMDPENRRMLKVTIEDAIAADHLFMCLMGDDVEPRRQFIEENALTVT NLDI >Mature_883_residues SESNHNTEQALVQGLPEGVDATDLPAGAEPEEYSSKNIRVLRGLEAVRVRPGMYIGDTDDGTGLHHMVFEVVDNSIDEAL AGHCDQIDIIIHEDESVSVMDNGRGIPIDVHPEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLEM NIWREGHHYQQTYSDGVPDSDIQELEKTNKTGTQIRFYPSPEVFTGTVFDFDILAKRLRELSFLNSGVRIVLTDERTDKQ HVFEHKGGLSEFVAYINSGKEGLNDVFHFVSEQEDGIVVEAALQWTDTYNEKVLCFTNNIPQKDGGTHLSGFRSALTRCL NSYMDNEKLVKKEKVNTTGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMHDKFNDYLLEHPGAAKSIAN KIIDAARARDAARKARELTRRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILNV ERARFDKMLSSAEVGTLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLTFFFRQTPELIERGYVYIAQPP LYKIKKGRQELYLKDDEALKAYLLSSTIDEMNLHISEDAPAITGQALETLLNDYNQTQLVKARLQIRYPAILLDALTHVP KLTTDMTYDFDAMTEWKQQLQAQLEHFGSDLRPSIELDNIHAPRTDELSEAAKDANNQFKFKIGAKVDSPNSDIEDAEVV EGQDSDMLSTKPQWLPRITIYVHNLAQHYLLDAGFFNSGEYARLMRLSKQWNTLLTESAFIRRDTTAGGSKDTLIRNFDH LWQQVMQEARRGLSVQRYKGLGEMNADQLWDTTMDPENRRMLKVTIEDAIAADHLFMCLMGDDVEPRRQFIEENALTVTN LDI
Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]
COG id: COG0187
COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI19913406, Length=570, Percent_Identity=25.7894736842105, Blast_Score=137, Evalue=7e-32, Organism=Homo sapiens, GI19913408, Length=593, Percent_Identity=25.6323777403035, Blast_Score=136, Evalue=1e-31, Organism=Escherichia coli, GI48994957, Length=855, Percent_Identity=60, Blast_Score=994, Evalue=0.0, Organism=Escherichia coli, GI1789408, Length=556, Percent_Identity=40.1079136690648, Blast_Score=398, Evalue=1e-112, Organism=Caenorhabditis elegans, GI17535065, Length=571, Percent_Identity=24.8686514886165, Blast_Score=143, Evalue=4e-34, Organism=Caenorhabditis elegans, GI212645845, Length=508, Percent_Identity=23.6220472440945, Blast_Score=135, Evalue=1e-31, Organism=Caenorhabditis elegans, GI212645657, Length=161, Percent_Identity=34.1614906832298, Blast_Score=75, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6324241, Length=586, Percent_Identity=24.7440273037543, Blast_Score=121, Evalue=4e-28, Organism=Drosophila melanogaster, GI17136538, Length=576, Percent_Identity=26.5625, Blast_Score=142, Evalue=1e-33,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003594 - InterPro: IPR011557 - InterPro: IPR020568 - InterPro: IPR014721 - InterPro: IPR001241 - InterPro: IPR013759 - InterPro: IPR002288 - InterPro: IPR013506 - InterPro: IPR013760 - InterPro: IPR018522 - InterPro: IPR006171 [H]
Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]
EC number: =5.99.1.3 [H]
Molecular weight: Translated: 98783; Mature: 98652
Theoretical pI: Translated: 4.96; Mature: 4.96
Prosite motif: PS00177 TOPOISOMERASE_II
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSESNHNTEQALVQGLPEGVDATDLPAGAEPEEYSSKNIRVLRGLEAVRVRPGMYIGDTD CCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCHHHEECCCEEECCCC DGTGLHHMVFEVVDNSIDEALAGHCDQIDIIIHEDESVSVMDNGRGIPIDVHPEEGVSAA CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCEEECCCCCCCHHH QVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLEMNIWREGHHYQQTYSDGVPD HHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC SDIQELEKTNKTGTQIRFYPSPEVFTGTVFDFDILAKRLRELSFLNSGVRIVLTDERTDK HHHHHHHHCCCCCCEEEEECCCCCEEECEECHHHHHHHHHHHHHHCCCCEEEEECCCCCH QHVFEHKGGLSEFVAYINSGKEGLNDVFHFVSEQEDGIVVEAALQWTDTYNEKVLCFTNN HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEECC IPQKDGGTHLSGFRSALTRCLNSYMDNEKLVKKEKVNTTGDDAREGLTAIVSVKVPDPKF CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCEEEEEEECCCCCC SSQTKDKLVSSEVKSAVESAMHDKFNDYLLEHPGAAKSIANKIIDAARARDAARKARELT CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH RRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN HHHCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEC VERARFDKMLSSAEVGTLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLT HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH FFFRQTPELIERGYVYIAQPPLYKIKKGRQELYLKDDEALKAYLLSSTIDEMNLHISEDA HHHHCCHHHHHCCEEEEECCCHHHHHCCCHHEEECCHHHHHHHHHHHHHHHCCEEECCCC PAITGQALETLLNDYNQTQLVKARLQIRYPAILLDALTHVPKLTTDMTYDFDAMTEWKQQ CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH LQAQLEHFGSDLRPSIELDNIHAPRTDELSEAAKDANNQFKFKIGAKVDSPNSDIEDAEV HHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEEECEECCCCCCCCCHHH VEGQDSDMLSTKPQWLPRITIYVHNLAQHYLLDAGFFNSGEYARLMRLSKQWNTLLTESA HCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH FIRRDTTAGGSKDTLIRNFDHLWQQVMQEARRGLSVQRYKGLGEMNADQLWDTTMDPENR HHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHCCCCCCCCCC RMLKVTIEDAIAADHLFMCLMGDDVEPRRQFIEENALTVTNLDI EEEEEEHHHHHHHHHHEEEEECCCCCHHHHHHHCCCEEEEECCC >Mature Secondary Structure SESNHNTEQALVQGLPEGVDATDLPAGAEPEEYSSKNIRVLRGLEAVRVRPGMYIGDTD CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCHHHEECCCEEECCCC DGTGLHHMVFEVVDNSIDEALAGHCDQIDIIIHEDESVSVMDNGRGIPIDVHPEEGVSAA CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCEEECCCCCCCHHH QVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLEMNIWREGHHYQQTYSDGVPD HHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC SDIQELEKTNKTGTQIRFYPSPEVFTGTVFDFDILAKRLRELSFLNSGVRIVLTDERTDK HHHHHHHHCCCCCCEEEEECCCCCEEECEECHHHHHHHHHHHHHHCCCCEEEEECCCCCH QHVFEHKGGLSEFVAYINSGKEGLNDVFHFVSEQEDGIVVEAALQWTDTYNEKVLCFTNN HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEECC IPQKDGGTHLSGFRSALTRCLNSYMDNEKLVKKEKVNTTGDDAREGLTAIVSVKVPDPKF CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCEEEEEEECCCCCC SSQTKDKLVSSEVKSAVESAMHDKFNDYLLEHPGAAKSIANKIIDAARARDAARKARELT CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH RRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN HHHCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCEEC VERARFDKMLSSAEVGTLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLT HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH FFFRQTPELIERGYVYIAQPPLYKIKKGRQELYLKDDEALKAYLLSSTIDEMNLHISEDA HHHHCCHHHHHCCEEEEECCCHHHHHCCCHHEEECCHHHHHHHHHHHHHHHCCEEECCCC PAITGQALETLLNDYNQTQLVKARLQIRYPAILLDALTHVPKLTTDMTYDFDAMTEWKQQ CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH LQAQLEHFGSDLRPSIELDNIHAPRTDELSEAAKDANNQFKFKIGAKVDSPNSDIEDAEV HHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEEECEECCCCCCCCCHHH VEGQDSDMLSTKPQWLPRITIYVHNLAQHYLLDAGFFNSGEYARLMRLSKQWNTLLTESA HCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH FIRRDTTAGGSKDTLIRNFDHLWQQVMQEARRGLSVQRYKGLGEMNADQLWDTTMDPENR HHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHCCCCCCCCCC RMLKVTIEDAIAADHLFMCLMGDDVEPRRQFIEENALTVTNLDI EEEEEEHHHHHHHHHHEEEEECCCCCHHHHHHHCCCEEEEECCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12620739; 9464408 [H]