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The map label for this gene is cheR [C]

Identifier: 148550790

GI number: 148550790

Start: 264191

End: 265015

Strand: Direct

Name: cheR [C]

Synonym: Swit_5354

Alternate gene names: 148550790

Gene position: 264191-265015 (Clockwise)

Preceding gene: 148550789

Following gene: 148550791

Centisome position: 85.16

GC content: 60.48

Gene sequence:

>825_bases
GTGACAGGCGGCCTCGAGGATCTGGAGATCGGCCTCCTGCTGGAAGCGCTCTACCAGCGTTATCATTACGACTTTCGCCC
CTATGCGCGCGCCTCGATCAAGCGGCGCCTCGTGCAGGCGCGGAGCCAGTTGGGCTTTGCGAGTTTTTCGGCGATGCAGG
AAAGCCTGCTCCATGATGAGCAGACGCTGCCGCGCCTGCTCAATTACCTGACCGTGCAGGTCAGTGAGATGTTCCGCGAC
CCGGCCTACTACAAGGCGCTGCGCGAACGCGTCATCCCGCACCTTAGAACCTATCCCTCGCTCAAGGTCTGGGTAGCCGG
CTGCAGCAACGGCGAGGAGCTCTATTCGCTTTCCATCCTCTTCCGCGAGGAGGGTCTCGGCGCGCGGACGATCTTCTATG
CGACCGATATCAATCCCGATGCCTTACGGGCGGCGCAGGCGGGGATCTATCCGCTCGACCAGATAAAGAAATATACGGCC
AATCACCAGAAGTCGGGCGCGCGCACGTCGCTATCTGACTATTATACGGCCGACTACGGGCATGCCGTGTTCGACAAGAC
GCTCAGGAGCCAGGTCGTCTTTTCCGACCATAGCCTGGTGACCGACGCGGTGTTTGCGGAGATGCATCTCATCTCGTGCC
GCAACGTGCTCATCTATTTCGATCGCGAGCTCCAGGATCGCGCCATCGGACTGTTCCGCGATTCCCTCGCCCGTAAGGGA
TTTCTGGGGCTCGGCTCGAAGGAGAGCCTGCGCTTTTCCCGGCACGCGGCGGCCTTTGCCGATTTCGATACAGCAGAGAA
GATCTATCAGCGGGTGGACGGATGA

Upstream 100 bases:

>100_bases
TGCCTGGAAGCGGGCGCCAACGACTATATGGCCAAGCCCATAGACGTCGATAAACTTTTGTCGCTGGTGCGCGTCTGGAT
GCCCCGATGAGGAGATCTCG

Downstream 100 bases:

>100_bases
GCCGGGATCCGATCAGGGCGGTCGTCATCGGCGCCTCGGCCGGCGCGGTGCAGGTCCTGCTGGAAATCCTGCCCGCGCTC
CCGCCAACGTTCCCCGTCCC

Product: MCP methyltransferase, CheR-type

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTGGLEDLEIGLLLEALYQRYHYDFRPYARASIKRRLVQARSQLGFASFSAMQESLLHDEQTLPRLLNYLTVQVSEMFRD
PAYYKALRERVIPHLRTYPSLKVWVAGCSNGEELYSLSILFREEGLGARTIFYATDINPDALRAAQAGIYPLDQIKKYTA
NHQKSGARTSLSDYYTADYGHAVFDKTLRSQVVFSDHSLVTDAVFAEMHLISCRNVLIYFDRELQDRAIGLFRDSLARKG
FLGLGSKESLRFSRHAAAFADFDTAEKIYQRVDG

Sequences:

>Translated_274_residues
MTGGLEDLEIGLLLEALYQRYHYDFRPYARASIKRRLVQARSQLGFASFSAMQESLLHDEQTLPRLLNYLTVQVSEMFRD
PAYYKALRERVIPHLRTYPSLKVWVAGCSNGEELYSLSILFREEGLGARTIFYATDINPDALRAAQAGIYPLDQIKKYTA
NHQKSGARTSLSDYYTADYGHAVFDKTLRSQVVFSDHSLVTDAVFAEMHLISCRNVLIYFDRELQDRAIGLFRDSLARKG
FLGLGSKESLRFSRHAAAFADFDTAEKIYQRVDG
>Mature_273_residues
TGGLEDLEIGLLLEALYQRYHYDFRPYARASIKRRLVQARSQLGFASFSAMQESLLHDEQTLPRLLNYLTVQVSEMFRDP
AYYKALRERVIPHLRTYPSLKVWVAGCSNGEELYSLSILFREEGLGARTIFYATDINPDALRAAQAGIYPLDQIKKYTAN
HQKSGARTSLSDYYTADYGHAVFDKTLRSQVVFSDHSLVTDAVFAEMHLISCRNVLIYFDRELQDRAIGLFRDSLARKGF
LGLGSKESLRFSRHAAAFADFDTAEKIYQRVDG

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=258, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80

Molecular weight: Translated: 31280; Mature: 31149

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGLEDLEIGLLLEALYQRYHYDFRPYARASIKRRLVQARSQLGFASFSAMQESLLHDE
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH
QTLPRLLNYLTVQVSEMFRDPAYYKALRERVIPHLRTYPSLKVWVAGCSNGEELYSLSIL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEEEE
FREEGLGARTIFYATDINPDALRAAQAGIYPLDQIKKYTANHQKSGARTSLSDYYTADYG
EECCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCC
HAVFDKTLRSQVVFSDHSLVTDAVFAEMHLISCRNVLIYFDRELQDRAIGLFRDSLARKG
HHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCC
FLGLGSKESLRFSRHAAAFADFDTAEKIYQRVDG
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure 
TGGLEDLEIGLLLEALYQRYHYDFRPYARASIKRRLVQARSQLGFASFSAMQESLLHDE
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH
QTLPRLLNYLTVQVSEMFRDPAYYKALRERVIPHLRTYPSLKVWVAGCSNGEELYSLSIL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHEEEEEE
FREEGLGARTIFYATDINPDALRAAQAGIYPLDQIKKYTANHQKSGARTSLSDYYTADYG
EECCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCC
HAVFDKTLRSQVVFSDHSLVTDAVFAEMHLISCRNVLIYFDRELQDRAIGLFRDSLARKG
HHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCC
FLGLGSKESLRFSRHAAAFADFDTAEKIYQRVDG
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-adenosyl-L-methionine; protein L-glutamate

Specific reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]