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The map label for this gene is thlA [H]

Identifier: 148550745

GI number: 148550745

Start: 206125

End: 207321

Strand: Direct

Name: thlA [H]

Synonym: Swit_5309

Alternate gene names: 148550745

Gene position: 206125-207321 (Clockwise)

Preceding gene: 148550744

Following gene: 148550748

Centisome position: 66.44

GC content: 66.08

Gene sequence:

>1197_bases
TTGGAAGACGTTGTGATTCTGGCCGGTACGCGAACGGCGATCGGCGACTTTGGTGGTAGCCTGAAGGACGTCGCGCCCTG
GCAGCTTGGCCGTATCGTCATTTCCGAGGCGATCGCCCGCGCCGGGATCGCGGCCGAGGACGTCGGCCATGTCGTCATGG
GGCAGGTGATCCAGAGCGAGCCGCGCGACGCCTATCTCGCTCGCATCGCGGCGATCGCGTCCGGCATCCCGGACCGGACG
CCGGCGCTCACTTTGAACCGGCTATGCGGATCGAGTGTGCAGGCGATCATTTCCGCGGCGCAGATGATCGCCCTTGGAGA
ATGCGAGATTGCTGTGGCCGGGGGCGCGGAAAGCATGAGCCAGTCCCCCTATGTCACCAAGGCAATGCGCTGGGGACGGC
GCCTGGGCGACGAGGTGATGGTCGATGCACTGAGCGCGGTTCTAACCGATCCGTTTGGCGCGGGGCATATGGGGGTCACC
GCCGAGAATGTCGCCGCGCGCCACGGGATCAGCCGCGAGGCGCAGGATGCGCTAGCCGCGCAAAGCCATGCCCGAGCGGC
CCGGGCAATCGCGGAGGGTCGCTTCAAGTCCCAGATCGTGGCGGTCGAAGTAAGGGAGCGGGGAAAAGTCAGGTCGTTCG
ACGTCGACGAGCATGTCCGTCAGGACGTGTCCTTCGAAAGTCTGGCATCGCTCAAGCCGATTTTCAAACCCGAGGATGGT
ACCGTCACGGCCGGAAATGCGAGCGGCATCAACGACGGCGGCGCGGCCGTGATCCTCGCGTCAGAGCGGACGGCGCAGGC
CCGCGGTTTGCAGCCCATGGGGCGGGTCGTCGCCTGGGGCCATGCCGGCGTCGCGCCGGAAGTCATGGGACTGGGCCCGG
TCGAAGCCGTACCGATCGCGCTAGCCCGTGCCGGGCTGACTCTGGCCGACATGGATGTCATCGAGGCGAACGAAGCTTTC
GCCGCCCAGGCCTGCGCCGTGAGCAAATTGCTGGGTTTCGACCCGGAGAAGGTCAATCCCAACGCAAGCGGGATCGGTCT
TGGCCATCCGGTGGGCGCAACCGGAGCCATGTTGACAGTGAAGGCGCTCTACGAGTTGACCCGCACCGGCGGCCGCTACG
CCCTCGTGACCATGTGCATCGGCGGCGGGCAAGGGATCGCGATGATCATCGAGCGGCTTGGCCCAAGGCTTTCGTGA

Upstream 100 bases:

>100_bases
TAGGTATACATCTTCATTTATCCGCTCTAATAAATCAGAACTGATGTTTAAAGCATCACCGCGCGGCTTGCATGCCAGCG
GCTCGCTGAAAGGAGCGATC

Downstream 100 bases:

>100_bases
TCCACCCGCTCGGCGGCCATTCTATTTGGCAGCGGCAGGCTAGGCTTTGATGCAAAGGCCGGCGCGCTTCCCCCCTAGGC
GGAACCGCCCGGAGGGGTGG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MEDVVILAGTRTAIGDFGGSLKDVAPWQLGRIVISEAIARAGIAAEDVGHVVMGQVIQSEPRDAYLARIAAIASGIPDRT
PALTLNRLCGSSVQAIISAAQMIALGECEIAVAGGAESMSQSPYVTKAMRWGRRLGDEVMVDALSAVLTDPFGAGHMGVT
AENVAARHGISREAQDALAAQSHARAARAIAEGRFKSQIVAVEVRERGKVRSFDVDEHVRQDVSFESLASLKPIFKPEDG
TVTAGNASGINDGGAAVILASERTAQARGLQPMGRVVAWGHAGVAPEVMGLGPVEAVPIALARAGLTLADMDVIEANEAF
AAQACAVSKLLGFDPEKVNPNASGIGLGHPVGATGAMLTVKALYELTRTGGRYALVTMCIGGGQGIAMIIERLGPRLS

Sequences:

>Translated_398_residues
MEDVVILAGTRTAIGDFGGSLKDVAPWQLGRIVISEAIARAGIAAEDVGHVVMGQVIQSEPRDAYLARIAAIASGIPDRT
PALTLNRLCGSSVQAIISAAQMIALGECEIAVAGGAESMSQSPYVTKAMRWGRRLGDEVMVDALSAVLTDPFGAGHMGVT
AENVAARHGISREAQDALAAQSHARAARAIAEGRFKSQIVAVEVRERGKVRSFDVDEHVRQDVSFESLASLKPIFKPEDG
TVTAGNASGINDGGAAVILASERTAQARGLQPMGRVVAWGHAGVAPEVMGLGPVEAVPIALARAGLTLADMDVIEANEAF
AAQACAVSKLLGFDPEKVNPNASGIGLGHPVGATGAMLTVKALYELTRTGGRYALVTMCIGGGQGIAMIIERLGPRLS
>Mature_398_residues
MEDVVILAGTRTAIGDFGGSLKDVAPWQLGRIVISEAIARAGIAAEDVGHVVMGQVIQSEPRDAYLARIAAIASGIPDRT
PALTLNRLCGSSVQAIISAAQMIALGECEIAVAGGAESMSQSPYVTKAMRWGRRLGDEVMVDALSAVLTDPFGAGHMGVT
AENVAARHGISREAQDALAAQSHARAARAIAEGRFKSQIVAVEVRERGKVRSFDVDEHVRQDVSFESLASLKPIFKPEDG
TVTAGNASGINDGGAAVILASERTAQARGLQPMGRVVAWGHAGVAPEVMGLGPVEAVPIALARAGLTLADMDVIEANEAF
AAQACAVSKLLGFDPEKVNPNASGIGLGHPVGATGAMLTVKALYELTRTGGRYALVTMCIGGGQGIAMIIERLGPRLS

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=44.4730077120823, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=42.7480916030534, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=41.6040100250627, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4557237, Length=396, Percent_Identity=37.8787878787879, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=34.7417840375587, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=30.7106598984772, Blast_Score=131, Evalue=9e-31,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=47.25, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082165, Length=397, Percent_Identity=45.3400503778337, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=44.8019801980198, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=39.8496240601504, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788683, Length=425, Percent_Identity=34.1176470588235, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=42.1994884910486, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=35.3846153846154, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=33.248730964467, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=434, Percent_Identity=30.6451612903226, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=397, Percent_Identity=28.463476070529, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=36.5239294710327, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=39.0862944162437, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=43.9086294416244, Blast_Score=320, Evalue=7e-88,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=43.3333333333333, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=37.8172588832487, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=33.8785046728972, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41114; Mature: 41114

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDVVILAGTRTAIGDFGGSLKDVAPWQLGRIVISEAIARAGIAAEDVGHVVMGQVIQSE
CCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PRDAYLARIAAIASGIPDRTPALTLNRLCGSSVQAIISAAQMIALGECEIAVAGGAESMS
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHEECCCEEEEECCHHHCC
QSPYVTKAMRWGRRLGDEVMVDALSAVLTDPFGAGHMGVTAENVAARHGISREAQDALAA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
QSHARAARAIAEGRFKSQIVAVEVRERGKVRSFDVDEHVRQDVSFESLASLKPIFKPEDG
HHHHHHHHHHHHCHHHHEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCC
TVTAGNASGINDGGAAVILASERTAQARGLQPMGRVVAWGHAGVAPEVMGLGPVEAVPIA
EEECCCCCCCCCCCEEEEEECCCCHHHCCCCHHHHEEEECCCCCCCHHHCCCCHHHHHHH
LARAGLTLADMDVIEANEAFAAQACAVSKLLGFDPEKVNPNASGIGLGHPVGATGAMLTV
HHHCCCEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KALYELTRTGGRYALVTMCIGGGQGIAMIIERLGPRLS
HHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MEDVVILAGTRTAIGDFGGSLKDVAPWQLGRIVISEAIARAGIAAEDVGHVVMGQVIQSE
CCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PRDAYLARIAAIASGIPDRTPALTLNRLCGSSVQAIISAAQMIALGECEIAVAGGAESMS
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHEECCCEEEEECCHHHCC
QSPYVTKAMRWGRRLGDEVMVDALSAVLTDPFGAGHMGVTAENVAARHGISREAQDALAA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
QSHARAARAIAEGRFKSQIVAVEVRERGKVRSFDVDEHVRQDVSFESLASLKPIFKPEDG
HHHHHHHHHHHHCHHHHEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCC
TVTAGNASGINDGGAAVILASERTAQARGLQPMGRVVAWGHAGVAPEVMGLGPVEAVPIA
EEECCCCCCCCCCCEEEEEECCCCHHHCCCCHHHHEEEECCCCCCCHHHCCCCHHHHHHH
LARAGLTLADMDVIEANEAFAAQACAVSKLLGFDPEKVNPNASGIGLGHPVGATGAMLTV
HHHCCCEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KALYELTRTGGRYALVTMCIGGGQGIAMIIERLGPRLS
HHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]