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The map label for this gene is traD [H]

Identifier: 148550661

GI number: 148550661

Start: 116438

End: 118729

Strand: Reverse

Name: traD [H]

Synonym: Swit_5224

Alternate gene names: 148550661

Gene position: 118729-116438 (Counterclockwise)

Preceding gene: 148550662

Following gene: 148550660

Centisome position: 38.27

GC content: 64.49

Gene sequence:

>2292_bases
ATGGCGCGTAAAAACGATATCCGCACAGACGGCCGGGCCATCCCCTTGACCCATCACAGCGCGCGCGGACGGGTGCAGCG
CAATTCGGGCAATTTTACCCGCGGCTCGCAGCTTCTCACCCACGAACTTCTGATGTGGTTCTCGGGTGCGAAACTGCCCA
TCCTGATGTGGTTCTTCCTCTTCGCGGCCGCCTGGTTCGTGATCATGTCGATCAAGCTCGACGAGCACGGCTTCCAGCTC
ATCTGCATGAAGATTTATGCCGCGCTCTGGAACTGGGTCGACCTCAACCCCAACAAGCGCGTCAACGTCACCCTGCCGAG
CGGCGAGGTCATGCGTACGGTGATGCCCGCGGTGCCTTATATCGCCGAGGTCATCCAGGCCTGGAAGATCGCGATGAAAG
GCCTTCTGGCGGCCTTCTTCGTATCCATCTTCATCACCATCCCGGTCGCACTCTGGTTCGTCGATCTTTCGCGCCGGCGG
GGCAAGACGATCCTTCAGGAACGCCACGAGCGCGGAGCCATGCTGGTCGCCCGCCACGTGCTTCTCAACGAGATCAGCCA
GCACAATTACGCGAAGTTCGAAGAGGAGGCGCAGGATCGCCTGCCCGGCCTCGCCCCGGCCAAGATTCTCGACATGCCGT
TTCGTGCACGCAAGGCGGCGGGCATCCATCACCCCTATACGCTTGCGGGCATCCCTTATCCGCATCGCACCGAGCAGTCG
CATACCATGCTCATCGGTACGACCGGATCGGGCAAGACGACCGAACTCAGGAGCCTGGTTTCGCAGGTGCGAGCCCGCGA
GGACACGGCCGTCATCTTCGATCTCACCGGCGCCTATGTCGAAGCCTTCTACGATTCCGCGCGCGATACGATCCTCAACC
CGATGGACGCGCGCTGCCCGGCCTGGTCGATCTTCAACGACTGCACCACGCATAGCGAATTCACTGCCGCAGCGGCGGCG
CTCATTCCCTCGGACGGCGGCTCTGCCGAACCCTTCTGGGCGCTCGCGGCGCGCACCCTCTTCATCGAGATGTGCATCCG
GCTCCAGGAGCGCGGCGCGACGACCAATCTCGCGCTCTCCGAGAACCTGATGACGGCCGACCTGAAGCGGGTTCACCGTT
TCCTTGCCAACACGATCGCGGACCCGCTCACCGCGCCGGAAGCCGCACGCATGGCGGAATCGATCCGCGCGGTCTTCAAT
ACCAATGCGCAGGTGCTGCGCTTCCTTCCCGACGAGGGGCCACGCTTCTCGATCCGCGACTGGACCACGACCGAGAAGAA
GCCCGGCGCGATCCTGTTCATCACCTCGAACTATGTCGATCTGGCGATGAACCGGGCGCTGCTGACGCTGTGGATGGACA
TCGCCATCAACCGCCTCATGACCATGCCGCGCACCCGCGATCTGCGCACCTGGTTCATGTTCGACGAACTCGGCGCACTC
CATCGTCTGCCCGCGATCGAGAATGGTCTCCAGACCGCCCGCGCGTTCGGCGGCGCGATGATCCTGGGGATCCACAGCTT
CGAAAAACTGATCGAGGTCTATGGCGAGCAGGGTGCCCGCAATCTCGCCTCGCTCGCGCGCTCCAAGCTGATGCTCGCAA
CCGCCGATCTCGACACCGCCGAGCAATGCGCGCGCTACATCGGCAACCGCGAAGTACGGCAGATGGATGAAGCCTATAGC
TACGGCTACAACAACACGCGCGACGCCGCGACGCTGACGCCGCGCAAGCAGGTCGAGCCGCTCGTCATCGCGGACGACAT
CACCAACCTGCCCTCGATGCATGGTTTCGTGAAATTCCCTGATGGCTTCCCCGCGGCGCGCATCCAGCTCCAGTGGAAAG
ACTACCCCCAGGTCGCCCAGGGCTTCATCGCCCGTCCCAATCTCCAGCCGGTGCGATCAAGGCGCGGAGAGGAGGCGTTC
GAGGGGAGCGATGGGGGCGAAGCGGGCGGGCGCGATGGCGCCGCGCAGGTGATCGGGGAGGTTGCCGATCCCGTCAATCT
GGCCAAGGAACTTGCCGCCAATATCCTGTCGGCCGAGGTCGAGGATGAGCATGATGAAGCGGCCCGCCGGGCGCAGCGTG
CGGCCGAGAGCGACGAGCGGCCAGCAACCCCCACGCACGCCGCGGACAGGGCACAGGCGGCGCGCGCTGCGACCGGGGAT
CCTCAGACCGTGCCGCGCGATCGCGACGGGCGGGGTCAACCTGCGCCAGTGCCCCAGGTCGAGGATCAGACGCTGGTCGA
GCTAAGGCAGGCGTTCGGGCGCGAGGACGACGGCATGGACATGGGGATCTGA

Upstream 100 bases:

>100_bases
GAGTTCCAGCAGCTCGCCCTCGACATCATCATCCGTGAGCAATTTCCCGAATTCCGCGACCGCATCGTCGCGGAAACCGA
CAAGCGCCTGGAGCAGTATC

Downstream 100 bases:

>100_bases
CATGCTGTCGGTCGCCTCGGTCCGCTCGGCTTCGGGCGCCGCGAATTATTTCGCGAAGGACGACTATTACACCGTCGAAG
GATCCTCCGAGATCAGCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MARKNDIRTDGRAIPLTHHSARGRVQRNSGNFTRGSQLLTHELLMWFSGAKLPILMWFFLFAAAWFVIMSIKLDEHGFQL
ICMKIYAALWNWVDLNPNKRVNVTLPSGEVMRTVMPAVPYIAEVIQAWKIAMKGLLAAFFVSIFITIPVALWFVDLSRRR
GKTILQERHERGAMLVARHVLLNEISQHNYAKFEEEAQDRLPGLAPAKILDMPFRARKAAGIHHPYTLAGIPYPHRTEQS
HTMLIGTTGSGKTTELRSLVSQVRAREDTAVIFDLTGAYVEAFYDSARDTILNPMDARCPAWSIFNDCTTHSEFTAAAAA
LIPSDGGSAEPFWALAARTLFIEMCIRLQERGATTNLALSENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFN
TNAQVLRFLPDEGPRFSIRDWTTTEKKPGAILFITSNYVDLAMNRALLTLWMDIAINRLMTMPRTRDLRTWFMFDELGAL
HRLPAIENGLQTARAFGGAMILGIHSFEKLIEVYGEQGARNLASLARSKLMLATADLDTAEQCARYIGNREVRQMDEAYS
YGYNNTRDAATLTPRKQVEPLVIADDITNLPSMHGFVKFPDGFPAARIQLQWKDYPQVAQGFIARPNLQPVRSRRGEEAF
EGSDGGEAGGRDGAAQVIGEVADPVNLAKELAANILSAEVEDEHDEAARRAQRAAESDERPATPTHAADRAQAARAATGD
PQTVPRDRDGRGQPAPVPQVEDQTLVELRQAFGREDDGMDMGI

Sequences:

>Translated_763_residues
MARKNDIRTDGRAIPLTHHSARGRVQRNSGNFTRGSQLLTHELLMWFSGAKLPILMWFFLFAAAWFVIMSIKLDEHGFQL
ICMKIYAALWNWVDLNPNKRVNVTLPSGEVMRTVMPAVPYIAEVIQAWKIAMKGLLAAFFVSIFITIPVALWFVDLSRRR
GKTILQERHERGAMLVARHVLLNEISQHNYAKFEEEAQDRLPGLAPAKILDMPFRARKAAGIHHPYTLAGIPYPHRTEQS
HTMLIGTTGSGKTTELRSLVSQVRAREDTAVIFDLTGAYVEAFYDSARDTILNPMDARCPAWSIFNDCTTHSEFTAAAAA
LIPSDGGSAEPFWALAARTLFIEMCIRLQERGATTNLALSENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFN
TNAQVLRFLPDEGPRFSIRDWTTTEKKPGAILFITSNYVDLAMNRALLTLWMDIAINRLMTMPRTRDLRTWFMFDELGAL
HRLPAIENGLQTARAFGGAMILGIHSFEKLIEVYGEQGARNLASLARSKLMLATADLDTAEQCARYIGNREVRQMDEAYS
YGYNNTRDAATLTPRKQVEPLVIADDITNLPSMHGFVKFPDGFPAARIQLQWKDYPQVAQGFIARPNLQPVRSRRGEEAF
EGSDGGEAGGRDGAAQVIGEVADPVNLAKELAANILSAEVEDEHDEAARRAQRAAESDERPATPTHAADRAQAARAATGD
PQTVPRDRDGRGQPAPVPQVEDQTLVELRQAFGREDDGMDMGI
>Mature_762_residues
ARKNDIRTDGRAIPLTHHSARGRVQRNSGNFTRGSQLLTHELLMWFSGAKLPILMWFFLFAAAWFVIMSIKLDEHGFQLI
CMKIYAALWNWVDLNPNKRVNVTLPSGEVMRTVMPAVPYIAEVIQAWKIAMKGLLAAFFVSIFITIPVALWFVDLSRRRG
KTILQERHERGAMLVARHVLLNEISQHNYAKFEEEAQDRLPGLAPAKILDMPFRARKAAGIHHPYTLAGIPYPHRTEQSH
TMLIGTTGSGKTTELRSLVSQVRAREDTAVIFDLTGAYVEAFYDSARDTILNPMDARCPAWSIFNDCTTHSEFTAAAAAL
IPSDGGSAEPFWALAARTLFIEMCIRLQERGATTNLALSENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNT
NAQVLRFLPDEGPRFSIRDWTTTEKKPGAILFITSNYVDLAMNRALLTLWMDIAINRLMTMPRTRDLRTWFMFDELGALH
RLPAIENGLQTARAFGGAMILGIHSFEKLIEVYGEQGARNLASLARSKLMLATADLDTAEQCARYIGNREVRQMDEAYSY
GYNNTRDAATLTPRKQVEPLVIADDITNLPSMHGFVKFPDGFPAARIQLQWKDYPQVAQGFIARPNLQPVRSRRGEEAFE
GSDGGEAGGRDGAAQVIGEVADPVNLAKELAANILSAEVEDEHDEAARRAQRAAESDERPATPTHAADRAQAARAATGDP
QTVPRDRDGRGQPAPVPQVEDQTLVELRQAFGREDDGMDMGI

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 84933; Mature: 84802

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKNDIRTDGRAIPLTHHSARGRVQRNSGNFTRGSQLLTHELLMWFSGAKLPILMWFFL
CCCCCCCCCCCCEECEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
FAAAWFVIMSIKLDEHGFQLICMKIYAALWNWVDLNPNKRVNVTLPSGEVMRTVMPAVPY
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCEECCCCCEEEEECCCHHHHHHHHHHHHH
IAEVIQAWKIAMKGLLAAFFVSIFITIPVALWFVDLSRRRGKTILQERHERGAMLVARHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
LLNEISQHNYAKFEEEAQDRLPGLAPAKILDMPFRARKAAGIHHPYTLAGIPYPHRTEQS
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
HTMLIGTTGSGKTTELRSLVSQVRAREDTAVIFDLTGAYVEAFYDSARDTILNPMDARCP
CEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
AWSIFNDCTTHSEFTAAAAALIPSDGGSAEPFWALAARTLFIEMCIRLQERGATTNLALS
CHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
ENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNTNAQVLRFLPDEGPRFSIRD
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCEEEECC
WTTTEKKPGAILFITSNYVDLAMNRALLTLWMDIAINRLMTMPRTRDLRTWFMFDELGAL
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HRLPAIENGLQTARAFGGAMILGIHSFEKLIEVYGEQGARNLASLARSKLMLATADLDTA
HHCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCHH
EQCARYIGNREVRQMDEAYSYGYNNTRDAATLTPRKQVEPLVIADDITNLPSMHGFVKFP
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCEEECC
DGFPAARIQLQWKDYPQVAQGFIARPNLQPVRSRRGEEAFEGSDGGEAGGRDGAAQVIGE
CCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHH
VADPVNLAKELAANILSAEVEDEHDEAARRAQRAAESDERPATPTHAADRAQAARAATGD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
PQTVPRDRDGRGQPAPVPQVEDQTLVELRQAFGREDDGMDMGI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ARKNDIRTDGRAIPLTHHSARGRVQRNSGNFTRGSQLLTHELLMWFSGAKLPILMWFFL
CCCCCCCCCCCEECEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
FAAAWFVIMSIKLDEHGFQLICMKIYAALWNWVDLNPNKRVNVTLPSGEVMRTVMPAVPY
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCEECCCCCEEEEECCCHHHHHHHHHHHHH
IAEVIQAWKIAMKGLLAAFFVSIFITIPVALWFVDLSRRRGKTILQERHERGAMLVARHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
LLNEISQHNYAKFEEEAQDRLPGLAPAKILDMPFRARKAAGIHHPYTLAGIPYPHRTEQS
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
HTMLIGTTGSGKTTELRSLVSQVRAREDTAVIFDLTGAYVEAFYDSARDTILNPMDARCP
CEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCC
AWSIFNDCTTHSEFTAAAAALIPSDGGSAEPFWALAARTLFIEMCIRLQERGATTNLALS
CHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
ENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNTNAQVLRFLPDEGPRFSIRD
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCEEEECC
WTTTEKKPGAILFITSNYVDLAMNRALLTLWMDIAINRLMTMPRTRDLRTWFMFDELGAL
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HRLPAIENGLQTARAFGGAMILGIHSFEKLIEVYGEQGARNLASLARSKLMLATADLDTA
HHCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCHH
EQCARYIGNREVRQMDEAYSYGYNNTRDAATLTPRKQVEPLVIADDITNLPSMHGFVKFP
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCEEECC
DGFPAARIQLQWKDYPQVAQGFIARPNLQPVRSRRGEEAFEGSDGGEAGGRDGAAQVIGE
CCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHH
VADPVNLAKELAANILSAEVEDEHDEAARRAQRAAESDERPATPTHAADRAQAARAATGD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
PQTVPRDRDGRGQPAPVPQVEDQTLVELRQAFGREDDGMDMGI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]