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The map label for this gene is gyrB [H]

Identifier: 148380730

GI number: 148380730

Start: 2930662

End: 2932617

Strand: Reverse

Name: gyrB [H]

Synonym: CBO2770

Alternate gene names: 148380730

Gene position: 2932617-2930662 (Counterclockwise)

Preceding gene: 148380731

Following gene: 148380729

Centisome position: 75.45

GC content: 30.73

Gene sequence:

>1956_bases
ATGGAGGAAAGAATAGATAACACGAAGGATTATGATGTTACCAAGTTAACCTCTTTGGAAAAATTAGAGCCTGTAAGAGT
AAGACCAGGAATGTATATAGGATCTACTGGGATAAAAGGTCTTCATCACTGTATTTGGGAGATATTAGATAATGCTATTG
ATGAAATTTCAAATGGATATGGAGATACTGCAGAGGTTATATTAAATAAAGATAAAAGTGTTACAGTTGTAGATAATGGA
AGAGGAATTCCTACAGGTATACATCCTATAAAAAAGAAGTCTGGTGTGGAAATGGTCTTTACAGAGCTTCATACAGGAGG
AAAATTTAATAATTCAATATATAAAACTTCTGGAGGACTGCATGGTGTTGGAGCATCAGTAGTTAATGCTTTATCTGAGT
GGACAGAGGTTGAAGTTTATCAGAATGATAAAATATATAAACAAAGATTTGAATATGCCTTAGATAAAAAACTAAATAAA
AAGATGCCAGGAACTCCTGTAACAAAACTTAAAATAGTAGGAGATACAGACAAAACTGGTACTAGAGTAACATTTAAACC
TGATAAAGAAGTATTTTCAACTATAGATTTTAAATTCGATGTTATAGATGAAAGACTACAAGAGTTAGCCTTTCAAAATA
AAGGGATAACTTTAAAACTTATAGACAATAGAAAAGAAGAGCCGGAAATAAAAGAATATCATTCTGAAAGAGGTCTTTTA
GACTTTATAGATTATTTAAATGAAAGTAAAACACCAATACATAATCCAGCAGTATTATTTGAGGGAGAAAAGGAGATAAA
TAATTTAAAAATGGAAGGGGAAATATGCTTCCAATTTACAGACTCTTCAACAGAAAATATAGCAAGTTATGTAAATAATA
TTCCAACCACAGAAGCTGGAACCCATGAAACTGGATTTAAAACAGGCATGACTAGAGCGTTTAAAGAAGGCGCAAAAAAG
TTAAGCCTTATAAAAGAAAAAGATAAAGAATTTGATGGCGATGATCTTAGAGAAGGAATTACAGCTATAGTTAGAATAAA
AATAAGTAATCCTGTGTTCGAAGGCCAAACTAAAACTAAGCTTGGAAATAATGAAGCTTATACTATGATGAATGATTTAG
CCTATAGTAAATTTTTAGAATGGATAGAAGATAATAAAGAAGTATCCACATTGATTATAAATAATGCTTTAGCTGCTGCA
GCTAGAAGAGAAAAAATAAAAAAAATAAATGAAGCAGAAAAGAAAAAAATAGGTAAAGGATTAGCACCGTTAGCAGGAAA
GATAGCTGTGTGTACTTTAAGAGATTCAAATGTAACAGAGTTTATAGTGGTAGAAGGAGATTCTGCGGGAGGTTCTGCAA
AACAAGCAAGAGATAGAAGATTTCAAACCATAATGCCTTCTAAGGGTAAAATAATGAATACAGAAAAACAAAAAATCGAA
AATGTATTAGGCAGTGAAGAATTAAAAATATTTAATACAGCTATAGGTACAGGTGTATTAGATAATTATAAAGAGGAAGA
CTTAAAATATGATAAGATAATAATATTAAGTGATGCGGATGTAGATGGATATCATATTAGAACTTTATGGATGACTTATA
TATATAGATATATGAAGTCTTTAGTAGCTAATGGTCATCTTTATATAGCTATGCCACCTTTGTATAAAGTTTACAAGAAA
GATAAGAAAGGCGAAAAGTCGGTTTATTGCTATAATGATGAAGAATTAGAAAAGGCTAAAAAACAAATTGGAAGGGGATA
CCTAATTCAAAGATATAAAGGATTAGGAGAAATGAATCCAGAACAATTATGGGAAACTACATTAAATCCTGAAACAAGAA
CACTTCAAAGAATAACTATAGAAGATGCCGCAAAAGCTGAAAAGATGGTATCTTTGCTTATGGGAGATGTAGTAGAACCT
AGAAAAAATTACTTATACAAATACGCAGAGTTTTAG

Upstream 100 bases:

>100_bases
GAATTATGTGGAATAAACTGTAAAACTTAAATAAAAATTAAAGAATATGGTTACTACTAAGAATTAATATAAACATTAAG
TATAGATTTGGAGGATAAAA

Downstream 100 bases:

>100_bases
ATAAAAGGGCTAAATAATATATATTTAGCCTTTGCCATGTAAATAAATATAAAAGCACAGGGAGGACTTTTAGATGGCTA
AAAAAATAGTAATACCTAAA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGYGDTAEVILNKDKSVTVVDNG
RGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGLHGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNK
KMPGTPVTKLKIVGDTDKTGTRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
DFIDYLNESKTPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAGTHETGFKTGMTRAFKEGAKK
LSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTKLGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAA
ARREKIKKINEAEKKKIGKGLAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQTIMPSKGKIMNTEKQKIE
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKSLVANGHLYIAMPPLYKVYKK
DKKGEKSVYCYNDEELEKAKKQIGRGYLIQRYKGLGEMNPEQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEP
RKNYLYKYAEF

Sequences:

>Translated_651_residues
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGYGDTAEVILNKDKSVTVVDNG
RGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGLHGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNK
KMPGTPVTKLKIVGDTDKTGTRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
DFIDYLNESKTPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAGTHETGFKTGMTRAFKEGAKK
LSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTKLGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAA
ARREKIKKINEAEKKKIGKGLAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQTIMPSKGKIMNTEKQKIE
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKSLVANGHLYIAMPPLYKVYKK
DKKGEKSVYCYNDEELEKAKKQIGRGYLIQRYKGLGEMNPEQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEP
RKNYLYKYAEF
>Mature_651_residues
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGYGDTAEVILNKDKSVTVVDNG
RGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGLHGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNK
KMPGTPVTKLKIVGDTDKTGTRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
DFIDYLNESKTPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAGTHETGFKTGMTRAFKEGAKK
LSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTKLGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAA
ARREKIKKINEAEKKKIGKGLAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQTIMPSKGKIMNTEKQKIE
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKSLVANGHLYIAMPPLYKVYKK
DKKGEKSVYCYNDEELEKAKKQIGRGYLIQRYKGLGEMNPEQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEP
RKNYLYKYAEF

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=622, Percent_Identity=26.3665594855305, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI19913406, Length=647, Percent_Identity=23.6476043276662, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI48994957, Length=580, Percent_Identity=41.3793103448276, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789408, Length=651, Percent_Identity=36.8663594470046, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535065, Length=675, Percent_Identity=24.7407407407407, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=26.5938069216758, Blast_Score=122, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6324241, Length=647, Percent_Identity=25.3477588871716, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17136538, Length=625, Percent_Identity=26.08, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 73804; Mature: 73804

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGY
CCCCCCCCCCCCHHHHCCHHHCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
GDTAEVILNKDKSVTVVDNGRGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGL
CCCCHHEEECCCEEEEEECCCCCCCCCCCHHCCCCHHEEEEHHHCCCCCCCCEEECCCCC
HGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNKKMPGTPVTKLKIVGDTDKTG
CCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
TRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
CEEEECCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCHH
DFIDYLNESKTPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAG
HHHHHHCCCCCCCCCCEEEEECCHHHCCEEECCEEEEEECCCCHHHHHHHHHCCCCCCCC
THETGFKTGMTRAFKEGAKKLSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCEEEEEEEECCCEECCCCCCC
LGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAAARREKIKKINEAEKKKIGKG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQTIMPSKGKIMNTEKQKIE
CCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEECCHHHHHH
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKS
HHHCCCHHEEHHHHHHCCHHCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LVANGHLYIAMPPLYKVYKKDKKGEKSVYCYNDEELEKAKKQIGRGYLIQRYKGLGEMNP
HHCCCEEEEEECHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHCCCCCCH
EQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEPRKNYLYKYAEF
HHHHHHCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGY
CCCCCCCCCCCCHHHHCCHHHCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
GDTAEVILNKDKSVTVVDNGRGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGL
CCCCHHEEECCCEEEEEECCCCCCCCCCCHHCCCCHHEEEEHHHCCCCCCCCEEECCCCC
HGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNKKMPGTPVTKLKIVGDTDKTG
CCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
TRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
CEEEECCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCHH
DFIDYLNESKTPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAG
HHHHHHCCCCCCCCCCEEEEECCHHHCCEEECCEEEEEECCCCHHHHHHHHHCCCCCCCC
THETGFKTGMTRAFKEGAKKLSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCEEEEEEEECCCEECCCCCCC
LGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAAARREKIKKINEAEKKKIGKG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQTIMPSKGKIMNTEKQKIE
CCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEECCHHHHHH
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKS
HHHCCCHHEEHHHHHHCCHHCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LVANGHLYIAMPPLYKVYKKDKKGEKSVYCYNDEELEKAKKQIGRGYLIQRYKGLGEMNP
HHCCCEEEEEECHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHCCCCCCH
EQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEPRKNYLYKYAEF
HHHHHHCCCCCHHHHEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]