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The map label for this gene is dat [H]

Identifier: 148380714

GI number: 148380714

Start: 2907592

End: 2908458

Strand: Reverse

Name: dat [H]

Synonym: CBO2756

Alternate gene names: 148380714

Gene position: 2908458-2907592 (Counterclockwise)

Preceding gene: 148380715

Following gene: 148380713

Centisome position: 74.83

GC content: 23.88

Gene sequence:

>867_bases
GTGATATTTAGAATAAATGGGGGATATATATTAATGGAATGTTTTAATAAATTTTTTATAGAGAATGAGAAAATAAAAGA
AATAAATCTATTTGATGAAAATTTCCTTAAAGAAGGAAAATCTCTTTATGAAGTTATAAGGATAATAGATGGAGCGCCTT
TGTTTTTAAAAAGCCATTTAAATAGATTTTACAACTCTGCAAAACTAGAAGAACTAAATTTATGGTTAGATGAAGAGGTA
ATAAAAGAAAAAATAAATAGACTTATAAAAATAAATAAAGTATCTATAGGCAATATAAAGTTAGTATTTAATTTTAATAA
AGAGAAGAATAACAAATTTTTATGCTATTTTTTAAAACATAATTATCCAGAGGATATAGAGTATAAAAATGGAGTAAGAA
CTATACTTTATCATGGAGAAAGAGAAAATCCAAATGCAAAGGTTATAAATATGGATTTTAGAAAAGCTGTAGGAGAAAAG
ATAAAAGAAGAGAAAGCTTATGAAGCAATACTAGTAGACAAAAATGGATACATAACCGAGGGAAGTAAATCTAATATATT
TATGATAAAAGATAGTAAGGTAATAACATCGCCTGTAGAAAAAGTATTACCAGGAATAACAAGACAAAATATAATAGATG
TATGTAAAAATCTAAATTTGGATATTGATGAAGAAAAAGTACATTATAAAGATATAGAAAAATTAGAGGGACTATTTATA
TCAGGTACATCACCAAAAGTTTTACCAATAAAATCTGTAGATGAAATGGAATTCAAATCTTCAGAGAATAAATTAATACT
AAGTATTATGGAAGGTTATAATAAAGCAATAGAGAAAGATATAAAAAGTTATAAAAGTAAAGAATAA

Upstream 100 bases:

>100_bases
GAAGTAGAATAAAATTTAATTCAAATTATATTCACTGATTGTGAATTTTAATTCAAACTAATTTAAGTGCTTTATAATAT
TAATACACAATATGAGGATT

Downstream 100 bases:

>100_bases
TCATTTTAAAAATCAAATGGATATCACAGTGTAATTTTATAAGGCAGATAGCACAGCAAATTTATTGATAGATTGACAAA
ATATTGGATAAAAAATATAA

Product: putative branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MIFRINGGYILMECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEELNLWLDEEV
IKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEK
IKEEKAYEAILVDKNGYITEGSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFI
SGTSPKVLPIKSVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE

Sequences:

>Translated_288_residues
MIFRINGGYILMECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEELNLWLDEEV
IKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEK
IKEEKAYEAILVDKNGYITEGSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFI
SGTSPKVLPIKSVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE
>Mature_288_residues
MIFRINGGYILMECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHLNRFYNSAKLEELNLWLDEEV
IKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKHNYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEK
IKEEKAYEAILVDKNGYITEGSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFI
SGTSPKVLPIKSVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=243, Percent_Identity=25.1028806584362, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 33657; Mature: 33657

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFRINGGYILMECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHL
CEEEEECCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NRFYNSAKLEELNLWLDEEVIKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKH
HHHHCCCCHHHHHCCCCHHHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCCEEEEEEEC
NYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAILVDKNGYITE
CCCCCCCCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHEEEEEECCCCEEEC
GSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFI
CCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEE
SGTSPKVLPIKSVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE
ECCCCCEEEECCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIFRINGGYILMECFNKFFIENEKIKEINLFDENFLKEGKSLYEVIRIIDGAPLFLKSHL
CEEEEECCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NRFYNSAKLEELNLWLDEEVIKEKINRLIKINKVSIGNIKLVFNFNKEKNNKFLCYFLKH
HHHHCCCCHHHHHCCCCHHHHHHHHHHHEEEEEEEECCEEEEEEECCCCCCCEEEEEEEC
NYPEDIEYKNGVRTILYHGERENPNAKVINMDFRKAVGEKIKEEKAYEAILVDKNGYITE
CCCCCCCCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHEEEEEECCCCEEEC
GSKSNIFMIKDSKVITSPVEKVLPGITRQNIIDVCKNLNLDIDEEKVHYKDIEKLEGLFI
CCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEE
SGTSPKVLPIKSVDEMEFKSSENKLILSIMEGYNKAIEKDIKSYKSKE
ECCCCCEEEECCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]