Definition Clostridium botulinum A str. ATCC 3502, complete genome.
Accession NC_009495
Length 3,886,916

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The map label for this gene is 148380711

Identifier: 148380711

GI number: 148380711

Start: 2902192

End: 2904225

Strand: Reverse

Name: 148380711

Synonym: CBO2753

Alternate gene names: NA

Gene position: 2904225-2902192 (Counterclockwise)

Preceding gene: 148380712

Following gene: 148380710

Centisome position: 74.72

GC content: 28.02

Gene sequence:

>2034_bases
ATGAAAAAGACAATTTATAGTTGCTCTACATTAGATAAGTGTATAAATAAGGCTTGTAAAGAACTAGGATTAGAAAAAGA
GGAATTAAATTATGAAATTATAGAAGAGAAACAAGGCTTTTTCATGAAAAAGACTACAATATTAGTTAATGCAGAAGAAA
AATTAATGAATATGAAAGAAGATAAAGCTACTATAGAAAATAACCTAGAGGAAAATATAGAACATAATGAGGAAAAAATA
GAAATAGAAAAAGAACCAATAGCTAAAGAAACTATAGCAGTTAATGATGGAAAGGTAAGAATAGTAAAAGGACAAGTAAT
AGTTACAAATCCTAAAGAGGGTGGGAAACCAGCAGCTGTAGCTCCAGGGGATAATGTAACTATATTAATAGATGGAGACA
GAATAAAATCCAGAAAAGAAATCTTTGAAGAAAATAAAATAGAAGTTATTTTCGAAGAAGTTAAAGCTCATAGAGAGTTA
AATGTTAAAACGGATCAAAATAATATGGAAGCTTATATTTCTGTAATATATAAGCCGGAAATAAAGTATGGATTGAAAGA
TGTAGAAGATAAAAACTATTTAATATTAAACTCTTGTAAAATGGAAGAAAAAGATCCTCCTTATTATACTGAAGAAGAAA
TTATAGAAGCTTTAAAAAAGATGGGGATAGTTTATGGAATAATAAAAGAAAATCTAGAGCAGTGTACAAAAAATAATTGT
ATAGAATTGCTAATAGCTAAAGGAAAAGAAACTATTAATGAAGAGGATGAAAGTATAGAGATTAAATTTACAACAGATAA
TCAAGAATTAAAATTAACGGAGGATAAAACAGGAAATGTAGACTTTAAAAGTATAGGTTCAATAGAATCTGTCAAGCCAG
GAGAGGTATTAGCTGTAAGAAAAAAAGGTAAAGAGGGACAAGATGGAATCGATATAAAAGGTATAGTAAAAAAATATAAA
CAAGGAAAAAAAATTATATTGAAACCAGGTCAAGGAACAATTTTAAGGGATGAAGATACCATAGAAGCTTCCATAAAAGG
AAAACCCTGTGTAAAATCCAATATATTTTATGTATATCAAGTCCACGAAGTTAAAGGTGATGTGGATATAAGTACAGGAA
ATATAACATTTGTAGGAGATGTAGTGGTACAGGGCTCCGTAAAGGAAGGTATGAAAGTAGAAGCAGGAAATTCTGTGGAA
ATAAAAAAACATGTAGAGAGAGCGGAGATAATTTCAAAGAGTAATTTAAATATAGATGGAAATATAATAAATTCTGATAT
ATACGGTGGTGGGGAAGATACTTTAAAGGTAATGGTATTAAATAAATTAGAAAAATTAAAGGATATTCTTACAGAACTAA
TTTCCGCTGTAGAAGAAATAAAGAGATTTAATTTACTTGGAGAAGGTAAAAAAGACGGAGAGATAATAAAAATCTTAATA
GAGAATAAGTTTAAAACTTTGACTAAAATATGTATAGCTATAATGGCTAATATAAATATGTGTAGAAGTGATGATGAGGA
AGATGAATTAGTAGGAATTATAAGGAAAAAGTTAATAGGGTTAGGTCCCGTTCATATAAAAAATTATAGGGAATTAGAAC
AAATAGTAAATCTTATAGAAGAAAAAATAAAGATGTGCAAGGAAGGATTATCATTACCCGTTAATGTTAATATTAGCTAT
TGTCAAGATTCTAAAATAAAAAGTACAGGAGATATTTATATAACGGGTAAAGGTGAATATATATCAAAATTAACAAGTAA
TAATAATGTTTACTTTACTAAAGATGATAGCGTAGCAAGAGGTGGGCACATAATAGCTAAAAATGAAATAAGATGTAAAG
AGGTAGGAAGTGAAGCAGGAGTTATTACTAAACTACAAATTTTACAAAAGGGAGATATATATGTAGATGTAGCCTATCAA
AATACAATATTTATTATAGGTGATAGGGAATACTTATTAGAAATACCAAGTAAAGATATACATGCTTATTTAGACAAAAA
AGGAGAGATTACCGTAGAAAAGTTTGTATTATAG

Upstream 100 bases:

>100_bases
ATTATTATATGATATAAAATATAGTTATATATACCTATATTTAGAATCTGTATATTCCTCAAAATAGGGGGCGGGATAAT
ATAATTTAAGGGGGATCAAT

Downstream 100 bases:

>100_bases
GAGGTTAATTTAGTAATGGATATAAGTGAAGCTAAAGTATTAATATTTAAAATAAATGGAGAATACTATGCCGCAGATAT
CATGGAAATAGAAAGAATAC

Product: hypothetical protein

Products: NA

Alternate protein names: None

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKKTIYSCSTLDKCINKACKELGLEKEELNYEIIEEKQGFFMKKTTILVNAEEKLMNMKEDKATIENNLEENIEHNEEKI
EIEKEPIAKETIAVNDGKVRIVKGQVIVTNPKEGGKPAAVAPGDNVTILIDGDRIKSRKEIFEENKIEVIFEEVKAHREL
NVKTDQNNMEAYISVIYKPEIKYGLKDVEDKNYLILNSCKMEEKDPPYYTEEEIIEALKKMGIVYGIIKENLEQCTKNNC
IELLIAKGKETINEEDESIEIKFTTDNQELKLTEDKTGNVDFKSIGSIESVKPGEVLAVRKKGKEGQDGIDIKGIVKKYK
QGKKIILKPGQGTILRDEDTIEASIKGKPCVKSNIFYVYQVHEVKGDVDISTGNITFVGDVVVQGSVKEGMKVEAGNSVE
IKKHVERAEIISKSNLNIDGNIINSDIYGGGEDTLKVMVLNKLEKLKDILTELISAVEEIKRFNLLGEGKKDGEIIKILI
ENKFKTLTKICIAIMANINMCRSDDEEDELVGIIRKKLIGLGPVHIKNYRELEQIVNLIEEKIKMCKEGLSLPVNVNISY
CQDSKIKSTGDIYITGKGEYISKLTSNNNVYFTKDDSVARGGHIIAKNEIRCKEVGSEAGVITKLQILQKGDIYVDVAYQ
NTIFIIGDREYLLEIPSKDIHAYLDKKGEITVEKFVL

Sequences:

>Translated_677_residues
MKKTIYSCSTLDKCINKACKELGLEKEELNYEIIEEKQGFFMKKTTILVNAEEKLMNMKEDKATIENNLEENIEHNEEKI
EIEKEPIAKETIAVNDGKVRIVKGQVIVTNPKEGGKPAAVAPGDNVTILIDGDRIKSRKEIFEENKIEVIFEEVKAHREL
NVKTDQNNMEAYISVIYKPEIKYGLKDVEDKNYLILNSCKMEEKDPPYYTEEEIIEALKKMGIVYGIIKENLEQCTKNNC
IELLIAKGKETINEEDESIEIKFTTDNQELKLTEDKTGNVDFKSIGSIESVKPGEVLAVRKKGKEGQDGIDIKGIVKKYK
QGKKIILKPGQGTILRDEDTIEASIKGKPCVKSNIFYVYQVHEVKGDVDISTGNITFVGDVVVQGSVKEGMKVEAGNSVE
IKKHVERAEIISKSNLNIDGNIINSDIYGGGEDTLKVMVLNKLEKLKDILTELISAVEEIKRFNLLGEGKKDGEIIKILI
ENKFKTLTKICIAIMANINMCRSDDEEDELVGIIRKKLIGLGPVHIKNYRELEQIVNLIEEKIKMCKEGLSLPVNVNISY
CQDSKIKSTGDIYITGKGEYISKLTSNNNVYFTKDDSVARGGHIIAKNEIRCKEVGSEAGVITKLQILQKGDIYVDVAYQ
NTIFIIGDREYLLEIPSKDIHAYLDKKGEITVEKFVL
>Mature_677_residues
MKKTIYSCSTLDKCINKACKELGLEKEELNYEIIEEKQGFFMKKTTILVNAEEKLMNMKEDKATIENNLEENIEHNEEKI
EIEKEPIAKETIAVNDGKVRIVKGQVIVTNPKEGGKPAAVAPGDNVTILIDGDRIKSRKEIFEENKIEVIFEEVKAHREL
NVKTDQNNMEAYISVIYKPEIKYGLKDVEDKNYLILNSCKMEEKDPPYYTEEEIIEALKKMGIVYGIIKENLEQCTKNNC
IELLIAKGKETINEEDESIEIKFTTDNQELKLTEDKTGNVDFKSIGSIESVKPGEVLAVRKKGKEGQDGIDIKGIVKKYK
QGKKIILKPGQGTILRDEDTIEASIKGKPCVKSNIFYVYQVHEVKGDVDISTGNITFVGDVVVQGSVKEGMKVEAGNSVE
IKKHVERAEIISKSNLNIDGNIINSDIYGGGEDTLKVMVLNKLEKLKDILTELISAVEEIKRFNLLGEGKKDGEIIKILI
ENKFKTLTKICIAIMANINMCRSDDEEDELVGIIRKKLIGLGPVHIKNYRELEQIVNLIEEKIKMCKEGLSLPVNVNISY
CQDSKIKSTGDIYITGKGEYISKLTSNNNVYFTKDDSVARGGHIIAKNEIRCKEVGSEAGVITKLQILQKGDIYVDVAYQ
NTIFIIGDREYLLEIPSKDIHAYLDKKGEITVEKFVL

Specific function: Unknown

COG id: COG1315

COG function: function code L; Predicted polymerase, most proteins contain PALM domain, HD hydrolase domain and Zn-ribbon domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 76235; Mature: 76235

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIYSCSTLDKCINKACKELGLEKEELNYEIIEEKQGFFMKKTTILVNAEEKLMNMKE
CCCCEECHHHHHHHHHHHHHHHCCCHHHCCCCEECCCCCEEEEEEEEEEECHHHHHCCHH
DKATIENNLEENIEHNEEKIEIEKEPIAKETIAVNDGKVRIVKGQVIVTNPKEGGKPAAV
HHHHHHHCHHHHHCCCCCEEEEECCCCCCEEEEECCCEEEEEEEEEEEECCCCCCCCCEE
APGDNVTILIDGDRIKSRKEIFEENKIEVIFEEVKAHRELNVKTDQNNMEAYISVIYKPE
CCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCC
IKYGLKDVEDKNYLILNSCKMEEKDPPYYTEEEIIEALKKMGIVYGIIKENLEQCTKNNC
HHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
IELLIAKGKETINEEDESIEIKFTTDNQELKLTEDKTGNVDFKSIGSIESVKPGEVLAVR
EEEEEECCCHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHCCCCCCCCCCCEEEEE
KKGKEGQDGIDIKGIVKKYKQGKKIILKPGQGTILRDEDTIEASIKGKPCVKSNIFYVYQ
CCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEECCCCEEEECCCCCCEECCEEEEEE
VHEVKGDVDISTGNITFVGDVVVQGSVKEGMKVEAGNSVEIKKHVERAEIISKSNLNIDG
EEEECCCEEEECCCEEEEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC
NIINSDIYGGGEDTLKVMVLNKLEKLKDILTELISAVEEIKRFNLLGEGKKDGEIIKILI
EEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
ENKFKTLTKICIAIMANINMCRSDDEEDELVGIIRKKLIGLGPVHIKNYRELEQIVNLIE
HHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
EKIKMCKEGLSLPVNVNISYCQDSKIKSTGDIYITGKGEYISKLTSNNNVYFTKDDSVAR
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHCCCCCEEEECCCCCCC
GGHIIAKNEIRCKEVGSEAGVITKLQILQKGDIYVDVAYQNTIFIIGDREYLLEIPSKDI
CCEEEEECCCCHHHCCCCCCEEEEEEEEECCCEEEEEEECCEEEEEECCCEEEECCCCHH
HAYLDKKGEITVEKFVL
HHHHCCCCCEEEEEECC
>Mature Secondary Structure
MKKTIYSCSTLDKCINKACKELGLEKEELNYEIIEEKQGFFMKKTTILVNAEEKLMNMKE
CCCCEECHHHHHHHHHHHHHHHCCCHHHCCCCEECCCCCEEEEEEEEEEECHHHHHCCHH
DKATIENNLEENIEHNEEKIEIEKEPIAKETIAVNDGKVRIVKGQVIVTNPKEGGKPAAV
HHHHHHHCHHHHHCCCCCEEEEECCCCCCEEEEECCCEEEEEEEEEEEECCCCCCCCCEE
APGDNVTILIDGDRIKSRKEIFEENKIEVIFEEVKAHRELNVKTDQNNMEAYISVIYKPE
CCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCC
IKYGLKDVEDKNYLILNSCKMEEKDPPYYTEEEIIEALKKMGIVYGIIKENLEQCTKNNC
HHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
IELLIAKGKETINEEDESIEIKFTTDNQELKLTEDKTGNVDFKSIGSIESVKPGEVLAVR
EEEEEECCCHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHCCCCCCCCCCCEEEEE
KKGKEGQDGIDIKGIVKKYKQGKKIILKPGQGTILRDEDTIEASIKGKPCVKSNIFYVYQ
CCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEECCCCEEEECCCCCCEECCEEEEEE
VHEVKGDVDISTGNITFVGDVVVQGSVKEGMKVEAGNSVEIKKHVERAEIISKSNLNIDG
EEEECCCEEEECCCEEEEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC
NIINSDIYGGGEDTLKVMVLNKLEKLKDILTELISAVEEIKRFNLLGEGKKDGEIIKILI
EEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
ENKFKTLTKICIAIMANINMCRSDDEEDELVGIIRKKLIGLGPVHIKNYRELEQIVNLIE
HHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
EKIKMCKEGLSLPVNVNISYCQDSKIKSTGDIYITGKGEYISKLTSNNNVYFTKDDSVAR
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHCCCCCEEEECCCCCCC
GGHIIAKNEIRCKEVGSEAGVITKLQILQKGDIYVDVAYQNTIFIIGDREYLLEIPSKDI
CCEEEEECCCCHHHCCCCCCEEEEEEEEECCCEEEEEEECCEEEEEECCCEEEECCCCHH
HAYLDKKGEITVEKFVL
HHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA