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The map label for this gene is fliM [H]

Identifier: 148380702

GI number: 148380702

Start: 2894735

End: 2895730

Strand: Reverse

Name: fliM [H]

Synonym: CBO2744

Alternate gene names: 148380702

Gene position: 2895730-2894735 (Counterclockwise)

Preceding gene: 148380703

Following gene: 148380701

Centisome position: 74.5

GC content: 31.43

Gene sequence:

>996_bases
ATGGCAGAAATATTGTCCCAGGGTGAAATAGATGCCCTTTTATCTGCTCTATCTTCAGGAGAAATAACACCAGACGAGGT
TCCCAAAGAAGAGGAAAAACAAAAAGTAAAACCCTATGATTTTAAAAGACCTCAAAAGTTCTCAAAGGATCACATAAGAA
CCTTAGAGCTTATACATGATAATTATGCAAGAATAATATCAAATTATTTAACAGCTCAAGTTAGAAGTAATGTGAAAGTT
AAGATAGAATCAGTTCAGCAGATAACTTATGAAGAATTTATTCACTCTGTGCCGAATCCAACAATTTTAACTATATTTAA
AATGCCACCTTTAAATGGGTCCATATTATTTGAAACAAATCCTCAATTTGTATTTCAAATGATAGATGTACTTTTAGGTG
GTAATGGTAATGGAACCTACAAAATGAGAGAGTTTACAGATATAGATAAAAATATAATGAAACAAATAAATACAGGACTT
ATAACAAACTTAAAATTAGCTTGGGAAGATGTAATGGAAGTGGATGTTGAAATAGAAAGCTTAGAAACTAATCCAGCTTT
AAATCAAACCCTAGCTCCAACAGAGCCTGTAGCTTTAATAACTTTCTCTGTGGAAATGGGAAAAAGTAATACTTTTATAA
ATATTTGTATACCATATTTGAGCATAGAAAAAGTTTTAGAAAAGCTAGTAGTTCAATACTGGTTTAGAGAAAATGATGAA
GAATCAATAGAACAATCTTCATCTAAAATAAAGGAAAGAATAAATATAGTTAGCATGGAACTGACTGCTGAATTAGGAAG
AACTTCAGTTACAGTAGAAGAATTTTTAAGATTAGCTGTAGGAGACGTGGTAGCCTTAGACACTAGAACTGAGGAACCTG
TCCATTTATTAATAGAAGAAGAACCAGGTTATTATGCTAAACCAGGAATTATAGGCAAGAATATAGGGTTACAGATACTA
GATCAAATAGATAAGGATGTGGAAAGTTATGAGTAA

Upstream 100 bases:

>100_bases
AGAATATATAGAAGGAATAATTAATTTTCAAGATAGAATAGTAACATTAATAGACATAGATAAATTATTATAATTCTCAA
AAAGGAAATGAGGGAAATAT

Downstream 100 bases:

>100_bases
CGGGTTTCTTTCACAGGAAGAAATAAATGCTCTTCTAAATGGAGATGGAAGCCAAGGGGAGGAAGCAAAAAAAGAAGCAA
CAGATACTGAACTACCAGAA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MAEILSQGEIDALLSALSSGEITPDEVPKEEEKQKVKPYDFKRPQKFSKDHIRTLELIHDNYARIISNYLTAQVRSNVKV
KIESVQQITYEEFIHSVPNPTILTIFKMPPLNGSILFETNPQFVFQMIDVLLGGNGNGTYKMREFTDIDKNIMKQINTGL
ITNLKLAWEDVMEVDVEIESLETNPALNQTLAPTEPVALITFSVEMGKSNTFINICIPYLSIEKVLEKLVVQYWFRENDE
ESIEQSSSKIKERINIVSMELTAELGRTSVTVEEFLRLAVGDVVALDTRTEEPVHLLIEEEPGYYAKPGIIGKNIGLQIL
DQIDKDVESYE

Sequences:

>Translated_331_residues
MAEILSQGEIDALLSALSSGEITPDEVPKEEEKQKVKPYDFKRPQKFSKDHIRTLELIHDNYARIISNYLTAQVRSNVKV
KIESVQQITYEEFIHSVPNPTILTIFKMPPLNGSILFETNPQFVFQMIDVLLGGNGNGTYKMREFTDIDKNIMKQINTGL
ITNLKLAWEDVMEVDVEIESLETNPALNQTLAPTEPVALITFSVEMGKSNTFINICIPYLSIEKVLEKLVVQYWFRENDE
ESIEQSSSKIKERINIVSMELTAELGRTSVTVEEFLRLAVGDVVALDTRTEEPVHLLIEEEPGYYAKPGIIGKNIGLQIL
DQIDKDVESYE
>Mature_330_residues
AEILSQGEIDALLSALSSGEITPDEVPKEEEKQKVKPYDFKRPQKFSKDHIRTLELIHDNYARIISNYLTAQVRSNVKVK
IESVQQITYEEFIHSVPNPTILTIFKMPPLNGSILFETNPQFVFQMIDVLLGGNGNGTYKMREFTDIDKNIMKQINTGLI
TNLKLAWEDVMEVDVEIESLETNPALNQTLAPTEPVALITFSVEMGKSNTFINICIPYLSIEKVLEKLVVQYWFRENDEE
SIEQSSSKIKERINIVSMELTAELGRTSVTVEEFLRLAVGDVVALDTRTEEPVHLLIEEEPGYYAKPGIIGKNIGLQILD
QIDKDVESYE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=287, Percent_Identity=27.5261324041812, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37586; Mature: 37455

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEILSQGEIDALLSALSSGEITPDEVPKEEEKQKVKPYDFKRPQKFSKDHIRTLELIHD
CCCHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NYARIISNYLTAQVRSNVKVKIESVQQITYEEFIHSVPNPTILTIFKMPPLNGSILFETN
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
PQFVFQMIDVLLGGNGNGTYKMREFTDIDKNIMKQINTGLITNLKLAWEDVMEVDVEIES
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHCEEEEE
LETNPALNQTLAPTEPVALITFSVEMGKSNTFINICIPYLSIEKVLEKLVVQYWFRENDE
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCH
ESIEQSSSKIKERINIVSMELTAELGRTSVTVEEFLRLAVGDVVALDTRTEEPVHLLIEE
HHHHHHHHHHHHHHHHHEEEHHHHHCCCEECHHHHHHHHHCCEEEECCCCCCCEEEEEEC
EPGYYAKPGIIGKNIGLQILDQIDKDVESYE
CCCCEECCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEILSQGEIDALLSALSSGEITPDEVPKEEEKQKVKPYDFKRPQKFSKDHIRTLELIHD
CCHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
NYARIISNYLTAQVRSNVKVKIESVQQITYEEFIHSVPNPTILTIFKMPPLNGSILFETN
HHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
PQFVFQMIDVLLGGNGNGTYKMREFTDIDKNIMKQINTGLITNLKLAWEDVMEVDVEIES
HHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHCEEEEE
LETNPALNQTLAPTEPVALITFSVEMGKSNTFINICIPYLSIEKVLEKLVVQYWFRENDE
CCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCH
ESIEQSSSKIKERINIVSMELTAELGRTSVTVEEFLRLAVGDVVALDTRTEEPVHLLIEE
HHHHHHHHHHHHHHHHHEEEHHHHHCCCEECHHHHHHHHHCCEEEECCCCCCCEEEEEEC
EPGYYAKPGIIGKNIGLQILDQIDKDVESYE
CCCCEECCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]