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The map label for this gene is galE [C]

Identifier: 148380683

GI number: 148380683

Start: 2875799

End: 2876824

Strand: Reverse

Name: galE [C]

Synonym: CBO2725

Alternate gene names: 148380683

Gene position: 2876824-2875799 (Counterclockwise)

Preceding gene: 148380684

Following gene: 148380682

Centisome position: 74.01

GC content: 27.97

Gene sequence:

>1026_bases
ATGAAGTATTACTCCAATAAAAATATACTAATAATAGGTGGAACTGGTACTATAGGTAAAGGGTTAGTAAAAGAGATAAT
AAAACAAAATCCTAAAGTTATAAGAATATTAAGTAGAGATGAATATAAACAATTCATTATGGAAAATGAAATAGACTCTA
GACTAAGAAGAAAATTTAGATTTTTGATAGGTGATGTAAGAGATTATGACAGAGTAGAAAGGGCTATGAAAGGTATTGAT
ATAGTATTTAATTTAGCGGCTATGAAACATGTGCCTTCCTGTGAATATAATCCTACAGAAGCTATAAAAACTAATATAAA
CGGCATGGAAAATGTTATAAAAGCAGCTATTTTCAATAATGTAGAATGTGTATTATTTACAAGTTCAGATAAAGCTATAA
ACCCAACTAATTCTTATGGTGCTACAAAACTTTTAGCAGAAAAATTAGTACAAGCTGCTAATTATAGTAAAGGACCTGTA
AAAACTAAATTTGTAGCAGTAAGATTCGGTAATGTAATGGGTTCTAGAGGATCCGTTATTCCTTTATTTAAAAAACAGAT
ATTAGAAAAAGGTAAAATTACAGTGACAGAACCTAATATGAGCAGATTTATGATGACATTAAAGGATGCAGTAAATTTAG
TTATGAAATCTTGTGAGATATCAAAGGGTGGAGAGCTCTTTGTACTTAAAATGCCAATTATTAAGCTTAAAGATTTGTCA
GAAGTTATTATAGAAGAGGTTTGTAAAAAAAATAATTTAGATAAAGATAAAATAGAAACAAAGATAATAGGTTTTAGAGC
TGGAGAAAGAATATATGAAGAGCTTATGACTTTACAAGAATCACACCATGCCAAAGATTTAGGTAATATGTATGTAGTGT
TTCCAACGGGATACTATAATGATTGGTATGAAAGTTATTATAATAAGTATGAGGATTGTAAAATAGGAAATTATTCTTCA
CATAATATAGGACCTATAAGCAAAGAAGAGGTTAGAAAAGTGCTAATAAATGAAAAATTAATATAA

Upstream 100 bases:

>100_bases
AAACTAAAAGATAATATTATTACTAAAATAGAAGAAATAAAAAAAATCATTGAAAATTAATCTTTTAGAAGAATACATAG
AAAGAAGGAGGTGTAATTTT

Downstream 100 bases:

>100_bases
GAAAATTTTATTAAATAAATTCAATTTATAAATAGTAACAAGCATTAATAAAAGAATTAGATTTTTATAATTTAGTATAG
TGCTTATACTAAAAAGTTTT

Product: polysaccharide biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKYYSNKNILIIGGTGTIGKGLVKEIIKQNPKVIRILSRDEYKQFIMENEIDSRLRRKFRFLIGDVRDYDRVERAMKGID
IVFNLAAMKHVPSCEYNPTEAIKTNINGMENVIKAAIFNNVECVLFTSSDKAINPTNSYGATKLLAEKLVQAANYSKGPV
KTKFVAVRFGNVMGSRGSVIPLFKKQILEKGKITVTEPNMSRFMMTLKDAVNLVMKSCEISKGGELFVLKMPIIKLKDLS
EVIIEEVCKKNNLDKDKIETKIIGFRAGERIYEELMTLQESHHAKDLGNMYVVFPTGYYNDWYESYYNKYEDCKIGNYSS
HNIGPISKEEVRKVLINEKLI

Sequences:

>Translated_341_residues
MKYYSNKNILIIGGTGTIGKGLVKEIIKQNPKVIRILSRDEYKQFIMENEIDSRLRRKFRFLIGDVRDYDRVERAMKGID
IVFNLAAMKHVPSCEYNPTEAIKTNINGMENVIKAAIFNNVECVLFTSSDKAINPTNSYGATKLLAEKLVQAANYSKGPV
KTKFVAVRFGNVMGSRGSVIPLFKKQILEKGKITVTEPNMSRFMMTLKDAVNLVMKSCEISKGGELFVLKMPIIKLKDLS
EVIIEEVCKKNNLDKDKIETKIIGFRAGERIYEELMTLQESHHAKDLGNMYVVFPTGYYNDWYESYYNKYEDCKIGNYSS
HNIGPISKEEVRKVLINEKLI
>Mature_341_residues
MKYYSNKNILIIGGTGTIGKGLVKEIIKQNPKVIRILSRDEYKQFIMENEIDSRLRRKFRFLIGDVRDYDRVERAMKGID
IVFNLAAMKHVPSCEYNPTEAIKTNINGMENVIKAAIFNNVECVLFTSSDKAINPTNSYGATKLLAEKLVQAANYSKGPV
KTKFVAVRFGNVMGSRGSVIPLFKKQILEKGKITVTEPNMSRFMMTLKDAVNLVMKSCEISKGGELFVLKMPIIKLKDLS
EVIIEEVCKKNNLDKDKIETKIIGFRAGERIYEELMTLQESHHAKDLGNMYVVFPTGYYNDWYESYYNKYEDCKIGNYSS
HNIGPISKEEVRKVLINEKLI

Specific function: Galactose metabolism; third step. [C]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38948; Mature: 38948

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYSNKNILIIGGTGTIGKGLVKEIIKQNPKVIRILSRDEYKQFIMENEIDSRLRRKFR
CCEECCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
FLIGDVRDYDRVERAMKGIDIVFNLAAMKHVPSCEYNPTEAIKTNINGMENVIKAAIFNN
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCC
VECVLFTSSDKAINPTNSYGATKLLAEKLVQAANYSKGPVKTKFVAVRFGNVMGSRGSVI
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHCCCCCCC
PLFKKQILEKGKITVTEPNMSRFMMTLKDAVNLVMKSCEISKGGELFVLKMPIIKLKDLS
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHH
EVIIEEVCKKNNLDKDKIETKIIGFRAGERIYEELMTLQESHHAKDLGNMYVVFPTGYYN
HHHHHHHHHHCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
DWYESYYNKYEDCKIGNYSSHNIGPISKEEVRKVLINEKLI
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYYSNKNILIIGGTGTIGKGLVKEIIKQNPKVIRILSRDEYKQFIMENEIDSRLRRKFR
CCEECCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
FLIGDVRDYDRVERAMKGIDIVFNLAAMKHVPSCEYNPTEAIKTNINGMENVIKAAIFNN
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCC
VECVLFTSSDKAINPTNSYGATKLLAEKLVQAANYSKGPVKTKFVAVRFGNVMGSRGSVI
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHCCCCCCC
PLFKKQILEKGKITVTEPNMSRFMMTLKDAVNLVMKSCEISKGGELFVLKMPIIKLKDLS
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHH
EVIIEEVCKKNNLDKDKIETKIIGFRAGERIYEELMTLQESHHAKDLGNMYVVFPTGYYN
HHHHHHHHHHCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
DWYESYYNKYEDCKIGNYSSHNIGPISKEEVRKVLINEKLI
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]