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The map label for this gene is rfbF [H]

Identifier: 148380676

GI number: 148380676

Start: 2867286

End: 2868062

Strand: Reverse

Name: rfbF [H]

Synonym: CBO2718

Alternate gene names: 148380676

Gene position: 2868062-2867286 (Counterclockwise)

Preceding gene: 148380677

Following gene: 148380675

Centisome position: 73.79

GC content: 31.53

Gene sequence:

>777_bases
TTGAAAGCAGTTATATTAGCTGGAGGATATGGTACACGATTAAGTGAAGAAACTATATTAAAGCCAAAGCCTATGGTTGA
AATAGGAGGCAAGCCAATACTTTGGCATATTATGAAGGCATATTCACATTATGGTATAAATGATTTTATAATATGTTTAG
GTTATAAAGGATATATAATAAAAGAATATTTTGCTAATTATTTTCTGCATCAATCAGATGTAACATTTGATATTGCCAAC
AATAAAATGAAAGTTCATGATAATCACTGTGAGCCATGGAAGGTAACTTTAGTGGATACTGGAACAGATACAATGACAGG
CGGAAGACTTAAAAAAGTTAGAAATTATATAGGAAATGAAACATTTTGCTTTACTTATGGTGATGGAGTAAGTGATGTAA
ATATAAGAAAAGTTATAGAGTTTCATAATGTTCAAAAGAAAAATGGTGTCTTAGCAACTTTAACAGCGGTAAATCCTCCT
GGAAGATATGGTGCCATTGATTTAAAGTATGAAAAAGTGAGTAATTTTAAAGAAAAACCTCAAGGAGAAGATAGTTGGAT
AAATGGGGGATACTTTGTAGTTGAACCAGAAGCTATTAATTATATAAAAGGTGATGAAACTTCATGGGAATCAGAACCAC
TTGAATTATTTGCGAGAAAAGAAAAATTAGCAGCATTTAAACATAAAGGTTTTTGGCTTCCAATGGATACATTAAGAGAT
AAAATGAGATTAGAAGAATTATGGAAGTCTGGACAAGCTCCATGGAAGGTGTGGTAG

Upstream 100 bases:

>100_bases
GTTTATAAACATTAACAATAATATCTTTGACTTGGGGTTGCTTAAAAAAGGATTAATAGAGGCTAGACATTTGAATTATT
AAAACGGGGAGGGAAATAAA

Downstream 100 bases:

>100_bases
AGTGATTTTAAGAGCAAAAACAATAGATTTATTTAAATCAAATAATTTAAATGAATGTCAAAAAAGTAATAGTGAGTTAA
ATATAAAAGAAATTAAGGAA

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKAVILAGGYGTRLSEETILKPKPMVEIGGKPILWHIMKAYSHYGINDFIICLGYKGYIIKEYFANYFLHQSDVTFDIAN
NKMKVHDNHCEPWKVTLVDTGTDTMTGGRLKKVRNYIGNETFCFTYGDGVSDVNIRKVIEFHNVQKKNGVLATLTAVNPP
GRYGAIDLKYEKVSNFKEKPQGEDSWINGGYFVVEPEAINYIKGDETSWESEPLELFARKEKLAAFKHKGFWLPMDTLRD
KMRLEELWKSGQAPWKVW

Sequences:

>Translated_258_residues
MKAVILAGGYGTRLSEETILKPKPMVEIGGKPILWHIMKAYSHYGINDFIICLGYKGYIIKEYFANYFLHQSDVTFDIAN
NKMKVHDNHCEPWKVTLVDTGTDTMTGGRLKKVRNYIGNETFCFTYGDGVSDVNIRKVIEFHNVQKKNGVLATLTAVNPP
GRYGAIDLKYEKVSNFKEKPQGEDSWINGGYFVVEPEAINYIKGDETSWESEPLELFARKEKLAAFKHKGFWLPMDTLRD
KMRLEELWKSGQAPWKVW
>Mature_258_residues
MKAVILAGGYGTRLSEETILKPKPMVEIGGKPILWHIMKAYSHYGINDFIICLGYKGYIIKEYFANYFLHQSDVTFDIAN
NKMKVHDNHCEPWKVTLVDTGTDTMTGGRLKKVRNYIGNETFCFTYGDGVSDVNIRKVIEFHNVQKKNGVLATLTAVNPP
GRYGAIDLKYEKVSNFKEKPQGEDSWINGGYFVVEPEAINYIKGDETSWESEPLELFARKEKLAAFKHKGFWLPMDTLRD
KMRLEELWKSGQAPWKVW

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=245, Percent_Identity=26.1224489795918, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI11761619, Length=245, Percent_Identity=26.1224489795918, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI133931050, Length=252, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320148, Length=247, Percent_Identity=27.5303643724696, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21355443, Length=248, Percent_Identity=27.4193548387097, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24644084, Length=248, Percent_Identity=27.4193548387097, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29615; Mature: 29615

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGYGTRLSEETILKPKPMVEIGGKPILWHIMKAYSHYGINDFIICLGYKGYII
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHH
KEYFANYFLHQSDVTFDIANNKMKVHDNHCEPWKVTLVDTGTDTMTGGRLKKVRNYIGNE
HHHHHHHHEECCCEEEEEECCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
TFCFTYGDGVSDVNIRKVIEFHNVQKKNGVLATLTAVNPPGRYGAIDLKYEKVSNFKEKP
EEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHHCHHCCC
QGEDSWINGGYFVVEPEAINYIKGDETSWESEPLELFARKEKLAAFKHKGFWLPMDTLRD
CCCCCEECCCEEEECCHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHH
KMRLEELWKSGQAPWKVW
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKAVILAGGYGTRLSEETILKPKPMVEIGGKPILWHIMKAYSHYGINDFIICLGYKGYII
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHH
KEYFANYFLHQSDVTFDIANNKMKVHDNHCEPWKVTLVDTGTDTMTGGRLKKVRNYIGNE
HHHHHHHHEECCCEEEEEECCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
TFCFTYGDGVSDVNIRKVIEFHNVQKKNGVLATLTAVNPPGRYGAIDLKYEKVSNFKEKP
EEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEHHHHHCHHCCC
QGEDSWINGGYFVVEPEAINYIKGDETSWESEPLELFARKEKLAAFKHKGFWLPMDTLRD
CCCCCEECCCEEEECCHHCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHH
KMRLEELWKSGQAPWKVW
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]