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The map label for this gene is cls [H]

Identifier: 148380632

GI number: 148380632

Start: 2821503

End: 2822936

Strand: Reverse

Name: cls [H]

Synonym: CBO2674

Alternate gene names: 148380632

Gene position: 2822936-2821503 (Counterclockwise)

Preceding gene: 148380634

Following gene: 148380631

Centisome position: 72.63

GC content: 26.92

Gene sequence:

>1434_bases
TTGGTGTCCTTTAAAAACATATTCTCTTTATTTTTTATTATAAATATAATTATATCTATAATATTAATAGTAATAGAGAG
AAAGAAGCCAGAAAAGACTATAGCATGGCTACTTATATTTTTTATATTTCCTCCTTTAGGACTATTTTTATATATTTTTT
TAGGTCGAAATTGGAAAAAACATAAATTACATGATACTACTAATATAGAAATAGAACAATTAGTACTTGAAGCTATGGGA
AAAGTAAAAAATCAGGAATATCATTCTTTAATAGAATTACTGTCTAGAAATAGCGAATCTCCCTTGTTCAGAGATAATAG
TATAAAAATATTTAAAGATGGTAATGAAAAATTTGAATATCTAAAAAAAGAGCTTTTAAAAGCTAAACATCATATACATT
TAGAGTATTATATAGTAAAAAATGACAATATAGGTAATGAAATAAAAGATATACTTATAAAAAAAGCAAAAGAAGGTGTA
AAGGTAAGGTTTATAATGGATAGGGTAGGATCTATAGGTATAAAAAGATTCTACATAAAAGAGCTTAAGGATAATGGTAT
AGATGTGGTGCAATATTCCTACTTTTTAGCTCCTCTTTTAAGGCATATAAATACTCAAATAAATTATAGAAATCATAGAA
AAATAGTAGTTATAGATGGTAAAACTGGATTTATAGGTGGTATAAATATAGGTGATGAATATTTAGGCAAAGGTAAATTA
GGTTATTGGAGAGATACTCACCTTATGATAAAGGGCGATTTTGTCATGGGACTTCAGGCAGTGTTTATGGATGACTTTGC
TACTATAAAGGCTGTAAATGATGAGATGTTTACTTATGGTGATAATTTTAAGGTTTATTTTCCTAAAATGCATGAGTACG
ATGGAAAGGTTATGCAACTTGTAAAAAGTGGACCAGATTCGCAGTTTCAATCTATAATGCAAACAATATTTAAGATGATA
ACTTTAGCTAAGGATCATATATACATTACTACACCATATTTTGTGCCTAATGATAGTATAATGAATGCTATAAAAGTAGC
ATCCTTAAGTGGAGTAGATGTAAGAATATTATTTCCAGGTAGATATGATCATTTAGTAGTATATTATGCCTCTAGAACTT
ATCTAGAAGAACTTATAAAATATGGAGTTAAAATTTATTTTTATGATGAGAACTCTTTTATTCATGCTAAAACTATTATG
GTAGATGGAGAAATTTGTACTGTAGGTACTGCTAATATGGATATAAGAAGTTTTGAATTAAATTATGAAATAAATGCGGT
TATATATGATGAGGAAACTACAGAAAATTTAGAGAACATTTTTTTCCAGGATTTAAAGCGTAGTAAAAGAATTTATAAAG
AAGATTTTAAAAATAGATCTTTAAGGGGCAGAATGTTGGAAGGTGTTGCTAGAATTTTTTCGGCTTTATTATAA

Upstream 100 bases:

>100_bases
AATATATGTTTGTTTAATTCATAGTAATTTTAATTTGATTATAGTATAATATATCTATTATATTAAAGATTCTATGTAAA
TACAATTTAAAGGAGGGATT

Downstream 100 bases:

>100_bases
AGTGTTTAAACAAAATTTAACACTAAAGAGTGAACTTTATATTACGATATTAATATAATGTAAAATATTTTGTAATTTAA
ATAATGTATTAAATATTATT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MVSFKNIFSLFFIINIIISIILIVIERKKPEKTIAWLLIFFIFPPLGLFLYIFLGRNWKKHKLHDTTNIEIEQLVLEAMG
KVKNQEYHSLIELLSRNSESPLFRDNSIKIFKDGNEKFEYLKKELLKAKHHIHLEYYIVKNDNIGNEIKDILIKKAKEGV
KVRFIMDRVGSIGIKRFYIKELKDNGIDVVQYSYFLAPLLRHINTQINYRNHRKIVVIDGKTGFIGGINIGDEYLGKGKL
GYWRDTHLMIKGDFVMGLQAVFMDDFATIKAVNDEMFTYGDNFKVYFPKMHEYDGKVMQLVKSGPDSQFQSIMQTIFKMI
TLAKDHIYITTPYFVPNDSIMNAIKVASLSGVDVRILFPGRYDHLVVYYASRTYLEELIKYGVKIYFYDENSFIHAKTIM
VDGEICTVGTANMDIRSFELNYEINAVIYDEETTENLENIFFQDLKRSKRIYKEDFKNRSLRGRMLEGVARIFSALL

Sequences:

>Translated_477_residues
MVSFKNIFSLFFIINIIISIILIVIERKKPEKTIAWLLIFFIFPPLGLFLYIFLGRNWKKHKLHDTTNIEIEQLVLEAMG
KVKNQEYHSLIELLSRNSESPLFRDNSIKIFKDGNEKFEYLKKELLKAKHHIHLEYYIVKNDNIGNEIKDILIKKAKEGV
KVRFIMDRVGSIGIKRFYIKELKDNGIDVVQYSYFLAPLLRHINTQINYRNHRKIVVIDGKTGFIGGINIGDEYLGKGKL
GYWRDTHLMIKGDFVMGLQAVFMDDFATIKAVNDEMFTYGDNFKVYFPKMHEYDGKVMQLVKSGPDSQFQSIMQTIFKMI
TLAKDHIYITTPYFVPNDSIMNAIKVASLSGVDVRILFPGRYDHLVVYYASRTYLEELIKYGVKIYFYDENSFIHAKTIM
VDGEICTVGTANMDIRSFELNYEINAVIYDEETTENLENIFFQDLKRSKRIYKEDFKNRSLRGRMLEGVARIFSALL
>Mature_477_residues
MVSFKNIFSLFFIINIIISIILIVIERKKPEKTIAWLLIFFIFPPLGLFLYIFLGRNWKKHKLHDTTNIEIEQLVLEAMG
KVKNQEYHSLIELLSRNSESPLFRDNSIKIFKDGNEKFEYLKKELLKAKHHIHLEYYIVKNDNIGNEIKDILIKKAKEGV
KVRFIMDRVGSIGIKRFYIKELKDNGIDVVQYSYFLAPLLRHINTQINYRNHRKIVVIDGKTGFIGGINIGDEYLGKGKL
GYWRDTHLMIKGDFVMGLQAVFMDDFATIKAVNDEMFTYGDNFKVYFPKMHEYDGKVMQLVKSGPDSQFQSIMQTIFKMI
TLAKDHIYITTPYFVPNDSIMNAIKVASLSGVDVRILFPGRYDHLVVYYASRTYLEELIKYGVKIYFYDENSFIHAKTIM
VDGEICTVGTANMDIRSFELNYEINAVIYDEETTENLENIFFQDLKRSKRIYKEDFKNRSLRGRMLEGVARIFSALL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=491, Percent_Identity=27.2912423625255, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1787007, Length=350, Percent_Identity=27.1428571428571, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081826, Length=383, Percent_Identity=26.3707571801567, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55797; Mature: 55797

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFKNIFSLFFIINIIISIILIVIERKKPEKTIAWLLIFFIFPPLGLFLYIFLGRNWKK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HKLHDTTNIEIEQLVLEAMGKVKNQEYHSLIELLSRNSESPLFRDNSIKIFKDGNEKFEY
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEECCCEEEEECCCHHHHH
LKKELLKAKHHIHLEYYIVKNDNIGNEIKDILIKKAKEGVKVRFIMDRVGSIGIKRFYIK
HHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHH
ELKDNGIDVVQYSYFLAPLLRHINTQINYRNHRKIVVIDGKTGFIGGINIGDEYLGKGKL
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCC
GYWRDTHLMIKGDFVMGLQAVFMDDFATIKAVNDEMFTYGDNFKVYFPKMHEYDGKVMQL
CEEECCEEEEECCHHHHHHHHHHHCCHHEEEECCCCEEECCCEEEEECCHHHCCHHHHHH
VKSGPDSQFQSIMQTIFKMITLAKDHIYITTPYFVPNDSIMNAIKVASLSGVDVRILFPG
HHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEECCCHHHHHHHHHEEECCCEEEEEECC
RYDHLVVYYASRTYLEELIKYGVKIYFYDENSFIHAKTIMVDGEICTVGTANMDIRSFEL
CCCEEEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCEEEEECCCCEEEEEEE
NYEINAVIYDEETTENLENIFFQDLKRSKRIYKEDFKNRSLRGRMLEGVARIFSALL
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MVSFKNIFSLFFIINIIISIILIVIERKKPEKTIAWLLIFFIFPPLGLFLYIFLGRNWKK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HKLHDTTNIEIEQLVLEAMGKVKNQEYHSLIELLSRNSESPLFRDNSIKIFKDGNEKFEY
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEECCCEEEEECCCHHHHH
LKKELLKAKHHIHLEYYIVKNDNIGNEIKDILIKKAKEGVKVRFIMDRVGSIGIKRFYIK
HHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCCHHHHHHH
ELKDNGIDVVQYSYFLAPLLRHINTQINYRNHRKIVVIDGKTGFIGGINIGDEYLGKGKL
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCHHHCCCCCC
GYWRDTHLMIKGDFVMGLQAVFMDDFATIKAVNDEMFTYGDNFKVYFPKMHEYDGKVMQL
CEEECCEEEEECCHHHHHHHHHHHCCHHEEEECCCCEEECCCEEEEECCHHHCCHHHHHH
VKSGPDSQFQSIMQTIFKMITLAKDHIYITTPYFVPNDSIMNAIKVASLSGVDVRILFPG
HHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEECCCHHHHHHHHHEEECCCEEEEEECC
RYDHLVVYYASRTYLEELIKYGVKIYFYDENSFIHAKTIMVDGEICTVGTANMDIRSFEL
CCCEEEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCEEEEECCCCEEEEEEE
NYEINAVIYDEETTENLENIFFQDLKRSKRIYKEDFKNRSLRGRMLEGVARIFSALL
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA