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The map label for this gene is argG

Identifier: 148380628

GI number: 148380628

Start: 2819130

End: 2820323

Strand: Reverse

Name: argG

Synonym: CBO2670

Alternate gene names: 148380628

Gene position: 2820323-2819130 (Counterclockwise)

Preceding gene: 148380629

Following gene: 148380627

Centisome position: 72.56

GC content: 32.58

Gene sequence:

>1194_bases
ATGAAAGAAAAAGTTGTGCTTGCATACTCAGGAGGATTAGATACATCAATAATAATTCCATGGCTTAAAGAAAATTATGA
CTTAGATGTTATAGCTGTCTGTGTAAATGTAGGTCAGGGGGATGATATGGATTATGTAAAGACAAAGGCAATAAAGTCTG
GTGCATCAAAAATATATGTGGAAGATGTAAAAGAGGAATTTGTAGTAGACTACTTGTATAAAGCAATAAAATCTGAAGCA
TTATATGAACAAGACTATATGTTAGGTACATCCTTTGCAAGACCTTTGATGGCTAAGAAATTAGTGGAAATAGCCCATAA
AGAGCAGGCTAAATATATATGTCATGGTTGTACAGGAAAGGGAAATGACCAAGTGCGTTTTGAAGTTGGAGTAAAGGCTC
AAGATCCTACTATAAAAATAATAGCACCTTGGAGAATTTGGGATATAAAATCTAGAGAAGATGCTATAGATTATGCTAAA
AAAGTTGGAGTTGAAGTTCCTGTAACTAAGAAAAAGATATATTCCGTAGATAAAAATTTATGGCATGTAAGTCACGAAGG
TGGAGATTTAGAAGATCTAAAAAATGAGCATAAGGAGGATATGTATTTTATGGTTACTCCACCGGAAAAGGCTAAAGATG
AGCCAACTTATTTGGAAATTTATTTTGAAAAGGGTGCACCAGTAAAAATAAATGGAGAGGTTTTAAATCCTGTAGATATA
ATAGATAAATTAAATACAATAGGTGGGGAGAACGGTATAGGCATAGCGGATATAATAGAAAATCGTTTGGTTGGTATGAA
GTCAAGAGGTATATATGAAACACCAGCCGGAACATTATTATATGCAGCTCATAAAAAATTAGAATCAGTAACCTTAGATA
AGTATACTTATCAATACAAAAAAATAGTTTCAGCTCAATATGGTGAGCTTGTATATAATGGACTATGGTTTACATCTTTA
AGAGAGGCTATAGATGCTTTTGTGGATAAAACTCAAGAAAATGTAACGGGTACAGTACAATTAAAACTGTATAAAGGGAA
TATAAAACCTTGCTCAGTAGATACAGAATATGCCTTATACGATGAAGGTATATCATCCTTTGGAGAGAGTGAACTTTACA
GTCATAAAGATGCAGAAGGCTTTATAAATTTATTTGGATTACCATCCAAAATAAAAGCGTTAAAAAATTTCTAG

Upstream 100 bases:

>100_bases
AATGAGGAATAAATTTTATAGGAAAATTGATTCCTATAAAAATGAAACTGGATTAAAGCCAGTTGTTAATAGAATTATAA
TATTTAAGGGGGAGTATTTT

Downstream 100 bases:

>100_bases
ACTAAATTGGAGGAACATACTATGAAACTTTGGGGAGGACGTTTTAAGGAAGAAGAAAGCAAACTTATGGAGGACTTTAA
TAGTTCTCTAAGTTTTGATA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYVEDVKEEFVVDYLYKAIKSEA
LYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGKGNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAK
KVGVEVPVTKKKIYSVDKNLWHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEVLNPVDI
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYKKIVSAQYGELVYNGLWFTSL
REAIDAFVDKTQENVTGTVQLKLYKGNIKPCSVDTEYALYDEGISSFGESELYSHKDAEGFINLFGLPSKIKALKNF

Sequences:

>Translated_397_residues
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYVEDVKEEFVVDYLYKAIKSEA
LYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGKGNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAK
KVGVEVPVTKKKIYSVDKNLWHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEVLNPVDI
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYKKIVSAQYGELVYNGLWFTSL
REAIDAFVDKTQENVTGTVQLKLYKGNIKPCSVDTEYALYDEGISSFGESELYSHKDAEGFINLFGLPSKIKALKNF
>Mature_397_residues
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYVEDVKEEFVVDYLYKAIKSEA
LYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGKGNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAK
KVGVEVPVTKKKIYSVDKNLWHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEVLNPVDI
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYKKIVSAQYGELVYNGLWFTSL
REAIDAFVDKTQENVTGTVQLKLYKGNIKPCSVDTEYALYDEGISSFGESELYSHKDAEGFINLFGLPSKIKALKNF

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=395, Percent_Identity=45.8227848101266, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI16950633, Length=395, Percent_Identity=45.8227848101266, Blast_Score=338, Evalue=7e-93,
Organism=Escherichia coli, GI1789563, Length=367, Percent_Identity=29.700272479564, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324514, Length=398, Percent_Identity=40.9547738693467, Blast_Score=293, Evalue=3e-80,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=42.2110552763819, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_CLOB1 (A7FWU6)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384913.1
- ProteinModelPortal:   A7FWU6
- SMR:   A7FWU6
- STRING:   A7FWU6
- GeneID:   5395732
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2612
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   CBOT441770:CLB_2612-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44732; Mature: 44732

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 86-86 BINDING 91-91 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 175-175 BINDING 184-184 BINDING 260-260 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYV
CCCCEEEEECCCCCCEEEEEHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEH
EDVKEEFVVDYLYKAIKSEALYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
GNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAKKVGVEVPVTKKKIYSVDKNL
CCCEEEEEEECCCCCCEEEEEECEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCE
WHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEVLNPVDI
EEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEECCCEECHHHH
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCEEHHHHHHHHHEEHHHHHHHHH
KIVSAQYGELVYNGLWFTSLREAIDAFVDKTQENVTGTVQLKLYKGNIKPCSVDTEYALY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHH
DEGISSFGESELYSHKDAEGFINLFGLPSKIKALKNF
HHHHHHCCCHHHHCCCCCCCCEEECCCCHHHHHHHCC
>Mature Secondary Structure
MKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCVNVGQGDDMDYVKTKAIKSGASKIYV
CCCCEEEEECCCCCCEEEEEHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEH
EDVKEEFVVDYLYKAIKSEALYEQDYMLGTSFARPLMAKKLVEIAHKEQAKYICHGCTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
GNDQVRFEVGVKAQDPTIKIIAPWRIWDIKSREDAIDYAKKVGVEVPVTKKKIYSVDKNL
CCCEEEEEEECCCCCCEEEEEECEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCE
WHVSHEGGDLEDLKNEHKEDMYFMVTPPEKAKDEPTYLEIYFEKGAPVKINGEVLNPVDI
EEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEECCCEECHHHH
IDKLNTIGGENGIGIADIIENRLVGMKSRGIYETPAGTLLYAAHKKLESVTLDKYTYQYK
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCCEEHHHHHHHHHEEHHHHHHHHH
KIVSAQYGELVYNGLWFTSLREAIDAFVDKTQENVTGTVQLKLYKGNIKPCSVDTEYALY
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHH
DEGISSFGESELYSHKDAEGFINLFGLPSKIKALKNF
HHHHHHCCCHHHHCCCCCCCCEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA