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The map label for this gene is fadH [C]

Identifier: 148379474

GI number: 148379474

Start: 1633625

End: 1634644

Strand: Direct

Name: fadH [C]

Synonym: CBO1503

Alternate gene names: 148379474

Gene position: 1633625-1634644 (Clockwise)

Preceding gene: 148379472

Following gene: 148379475

Centisome position: 42.03

GC content: 30.39

Gene sequence:

>1020_bases
ATGAAAAGTTTATTTGATAAAACCTGCATAAAAACAATGGAACTTAAAAATAGATTTGTTCGCTCAGCCACCTGGGAGGG
CATGGGCACAGAAGAAGGACATATTACGGAGAGATTACTTAATTTATATGAAAAATTAGCAAAGGGTGGAGTTGGATTAA
TAATAAGTAGTTACACTACTATATTTGATTATGATAAGCCAAGTCTTAGAATCCTTGGAATTTATGATGATAGCTTTATT
GAAGAATATAAGCTTTTGACAGATATAATTCATAAATATGAGGCTAAAGTATTAATGCAAATAGTCTTAGGGGAGAATTA
TATTAATAATGAAACAGGTAGTGAATTTTACGGATTAAGTGAAAATATGCCAGAAGATGATATAAAGGCTATAGTAAAAT
CTTTTGCAAAGTCAGCTAAAAGGGCTAAAGAGTCAGGTTTTGATGGAATTCAAATTCATGGAGCCCATGGATATTTTTTA
AGTAGAACATTAAGTCCTCTTTTTAATAAAAGAAAGGATAAATACGGTGGTTCAGTAGAAAAAAGAGGTGCACTAATACT
AGAGGTTTATGATGAGATAAGAAAAGCAGTGGGAAAAGATTTTCATATATCCATAAAAATTAATTGTTCTGATTTTGAAG
AGGGCGGAGCAACCTTTAAGGAATGTGAATTTGTTTGCAGGGAACTCTCTAAGAAAGGTATAGATTCTATAGAAATCAGC
GGTGGAGGAACAATTTGGACAGAAACTAATAAAAAGGAATCTATATATAAAGAGTATGCCTCAAAAATAGCTGAAGAAGT
AGATACTCCCATAATTTTAGTTGGTATGAATAGAAGTTATGACAATATGAATGAAATATTAAATAATAGTAAGATAGAAT
ATTTTTCAATGGCTAGACCTTTTATAAGGGAACCGGATTTAATAAATAAATTTGAAAAAGGTGAAAATAAAAAAGCTAAA
TGTATATCCTGTGGAAAATGTTGTGGTGAAAATGGAATAAGGTGTATATTCAATATGTAA

Upstream 100 bases:

>100_bases
GTTAAAAATTCTGTTTTAATTCTTTTGGTGAAAGTTCAACATTAAATCAAATTATGCTAATATAATAGTATAGAATTGAA
ACAAAACAGGGGGAACTTTT

Downstream 100 bases:

>100_bases
AATAAATATAAGGATGGGGAATGTAAGTAAAATTCTCCATCCTTATATTTATGTAAGCATTATTTTAAGATATATCAAAT
TAGTATTTTAAAAACAAAAG

Product: oxidoreductase, FAD/FMN-binding

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKSLFDKTCIKTMELKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTTIFDYDKPSLRILGIYDDSFI
EEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLSENMPEDDIKAIVKSFAKSAKRAKESGFDGIQIHGAHGYFL
SRTLSPLFNKRKDKYGGSVEKRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARPFIREPDLINKFEKGENKKAK
CISCGKCCGENGIRCIFNM

Sequences:

>Translated_339_residues
MKSLFDKTCIKTMELKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTTIFDYDKPSLRILGIYDDSFI
EEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLSENMPEDDIKAIVKSFAKSAKRAKESGFDGIQIHGAHGYFL
SRTLSPLFNKRKDKYGGSVEKRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARPFIREPDLINKFEKGENKKAK
CISCGKCCGENGIRCIFNM
>Mature_339_residues
MKSLFDKTCIKTMELKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTTIFDYDKPSLRILGIYDDSFI
EEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLSENMPEDDIKAIVKSFAKSAKRAKESGFDGIQIHGAHGYFL
SRTLSPLFNKRKDKYGGSVEKRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARPFIREPDLINKFEKGENKKAK
CISCGKCCGENGIRCIFNM

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=26.7806267806268, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787939, Length=347, Percent_Identity=24.207492795389, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=342, Percent_Identity=30.9941520467836, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17540738, Length=348, Percent_Identity=29.0229885057471, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17559804, Length=348, Percent_Identity=31.0344827586207, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI72001454, Length=347, Percent_Identity=29.6829971181556, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17559802, Length=340, Percent_Identity=30.8823529411765, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17565138, Length=333, Percent_Identity=28.8288288288288, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17566914, Length=334, Percent_Identity=28.4431137724551, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325086, Length=371, Percent_Identity=23.7196765498652, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=375, Percent_Identity=24.5333333333333, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 38486; Mature: 38486

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLFDKTCIKTMELKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
IFDYDKPSLRILGIYDDSFIEEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLS
EEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCC
ENMPEDDIKAIVKSFAKSAKRAKESGFDGIQIHGAHGYFLSRTLSPLFNKRKDKYGGSVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHH
KRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
HCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARP
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHHCCC
FIREPDLINKFEKGENKKAKCISCGKCCGENGIRCIFNM
CCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKSLFDKTCIKTMELKNRFVRSATWEGMGTEEGHITERLLNLYEKLAKGGVGLIISSYTT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
IFDYDKPSLRILGIYDDSFIEEYKLLTDIIHKYEAKVLMQIVLGENYINNETGSEFYGLS
EEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCC
ENMPEDDIKAIVKSFAKSAKRAKESGFDGIQIHGAHGYFLSRTLSPLFNKRKDKYGGSVE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHH
KRGALILEVYDEIRKAVGKDFHISIKINCSDFEEGGATFKECEFVCRELSKKGIDSIEIS
HCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
GGGTIWTETNKKESIYKEYASKIAEEVDTPIILVGMNRSYDNMNEILNNSKIEYFSMARP
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHHCCC
FIREPDLINKFEKGENKKAKCISCGKCCGENGIRCIFNM
CCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]