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The map label for this gene is mtbC [H]

Identifier: 148379465

GI number: 148379465

Start: 1625997

End: 1626659

Strand: Direct

Name: mtbC [H]

Synonym: CBO1494

Alternate gene names: 148379465

Gene position: 1625997-1626659 (Clockwise)

Preceding gene: 148379464

Following gene: 148379466

Centisome position: 41.83

GC content: 32.43

Gene sequence:

>663_bases
ATGAATAATGTATCAGAAGAGCTAATAAAAAAGTTATCAGAATCAGTAGTAGAAATGGAAGAAGAAGAAACAGTAGAATT
AGCCAAAGAGTGTATAGAAAATAATATTAGTGCCTATGAAGCTATAGATAAGGGTCTTGCCCATGGAATGAATAGAGCAG
GTGAATTATACGAAGAAGGAGAATATTATATTCCAGAATTATTGATGTGTTCTGATGCTATGTACTCAGGACTAGAAGTT
TTAAAACCTCACTTAAAAAAGAATGATTTAGGGAAAAAACATAAAATAGTCATAGGAGTAATACAAGGAGATACCCATGA
TATAGGAAAAAACTTAGTTAAAATAATGATGGAAACAGAGGGCTTTGAAGTTATAGATTTAGGAAGAGACGTACCACCGA
GAGATTTTGTAGACAAGGCTAAGGAGATACAAGCGGATATAATTTGTATGTCTACCCTTATGACTACAACTATGGATGGA
ATGGAAGAAGTTATAAAATTATTAAAAGAAGAAGGCATAAGAGACAAAGTAACAGTAATGATAGGAGGAGGTCCTATATC
TCAAAATTTTGCAGATAAAATAGGTGCAGATGTGTATACAACAGATGCTTCAAGAGCTGCAAAGCGTGCTAAAAAAATAG
TAGAAGAAAAAGTTAAATGTTAA

Upstream 100 bases:

>100_bases
ATATTAGGTCATAATTGTTTAAGAAAGACTATTAAGCCGCTGAAAAAACCTATAGTATGGGAAATAAAATTATAATAAAA
ATATCAAGGAGGAAAAGTAA

Downstream 100 bases:

>100_bases
TAATCTTATATTTAAATGAATAAGTTTTTTGTATATTTCACAGTTAATATAAGTAAAAATTTTAAAATATATGAGGTGCA
TTAAATGTTAACACCAAAAG

Product: dimethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNNVSEELIKKLSESVVEMEEEETVELAKECIENNISAYEAIDKGLAHGMNRAGELYEEGEYYIPELLMCSDAMYSGLEV
LKPHLKKNDLGKKHKIVIGVIQGDTHDIGKNLVKIMMETEGFEVIDLGRDVPPRDFVDKAKEIQADIICMSTLMTTTMDG
MEEVIKLLKEEGIRDKVTVMIGGGPISQNFADKIGADVYTTDASRAAKRAKKIVEEKVKC

Sequences:

>Translated_220_residues
MNNVSEELIKKLSESVVEMEEEETVELAKECIENNISAYEAIDKGLAHGMNRAGELYEEGEYYIPELLMCSDAMYSGLEV
LKPHLKKNDLGKKHKIVIGVIQGDTHDIGKNLVKIMMETEGFEVIDLGRDVPPRDFVDKAKEIQADIICMSTLMTTTMDG
MEEVIKLLKEEGIRDKVTVMIGGGPISQNFADKIGADVYTTDASRAAKRAKKIVEEKVKC
>Mature_220_residues
MNNVSEELIKKLSESVVEMEEEETVELAKECIENNISAYEAIDKGLAHGMNRAGELYEEGEYYIPELLMCSDAMYSGLEV
LKPHLKKNDLGKKHKIVIGVIQGDTHDIGKNLVKIMMETEGFEVIDLGRDVPPRDFVDKAKEIQADIICMSTLMTTTMDG
MEEVIKLLKEEGIRDKVTVMIGGGPISQNFADKIGADVYTTDASRAAKRAKKIVEEKVKC

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=231, Percent_Identity=27.7056277056277, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790450, Length=212, Percent_Identity=32.0754716981132, Blast_Score=107, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17535405, Length=194, Percent_Identity=28.8659793814433, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 24503; Mature: 24503

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVSEELIKKLSESVVEMEEEETVELAKECIENNISAYEAIDKGLAHGMNRAGELYEEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
EYYIPELLMCSDAMYSGLEVLKPHLKKNDLGKKHKIVIGVIQGDTHDIGKNLVKIMMETE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
GFEVIDLGRDVPPRDFVDKAKEIQADIICMSTLMTTTMDGMEEVIKLLKEEGIRDKVTVM
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IGGGPISQNFADKIGADVYTTDASRAAKRAKKIVEEKVKC
ECCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNVSEELIKKLSESVVEMEEEETVELAKECIENNISAYEAIDKGLAHGMNRAGELYEEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
EYYIPELLMCSDAMYSGLEVLKPHLKKNDLGKKHKIVIGVIQGDTHDIGKNLVKIMMETE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
GFEVIDLGRDVPPRDFVDKAKEIQADIICMSTLMTTTMDGMEEVIKLLKEEGIRDKVTVM
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IGGGPISQNFADKIGADVYTTDASRAAKRAKKIVEEKVKC
ECCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]