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The map label for this gene is mraW

Identifier: 148379426

GI number: 148379426

Start: 1586357

End: 1587286

Strand: Direct

Name: mraW

Synonym: CBO1454

Alternate gene names: 148379426

Gene position: 1586357-1587286 (Clockwise)

Preceding gene: 148379425

Following gene: 148379427

Centisome position: 40.81

GC content: 27.96

Gene sequence:

>930_bases
ATGGAGTTTAAACATATATCTGTATTATTAGAAGAGACTATAGATTCACTTAATATAAAGGAAGATGGTGTATATGTAGA
CTGTACTTTAGGTGGTGGAGGACATTCTAAAGAAATATTAAAAAAATTATCCCACAAGGGAAAATTAATAGGCATAGATC
AAGATACAAGTGCTATTAAAGCAGCTAAGGAAAGATTAAAAGATTATGAGAATGTAATTTATGTACATAATAATTTTTAT
AATATAGATAGTATTTTAGAAGAGCTAGATATAGATAAAGTTGATGGTATAATAATGGATTTAGGGGTATCTTCTTACCA
ATTAGATGAAGCTTCAAGAGGCTTTAGTTATATGAAAGATGCACCTTTAGATATGAGAATGAATAGAGAAGAAAATCTTT
CAGCCTATGGTGTAATAAATAATTATGAAGAAGAAGAATTGTTTAAAATATTAAAAAATTATGGGGAAGAAAAATTTTCT
AGAAAAATTGCTAGATTTATAGTAGAAAAAAGAACAGAAAATCCTATAGAAACTACAGGAGAATTAGTAGAGATAATAAG
AAAAGCTATTCCAGCTAAGTTTCAAAGAGAAGGACATCCTGCTAAAAGAACCTTTCAAGCTATAAGGATAGAAGTAAATA
AGGAACTTCAAATATTAAATAAAGCTATAGAAGACAGTGTAAATAGACTAAATAAAGATGGAAGATTAAGCATAATAACC
TTTCATTCTTTGGAGGATAGAATAGTAAAGGTGAAATTTAAAGAACTAGAAAAACCATGTACTTGCCCACCAAGCTTCCC
AATTTGTGTCTGTGGGAAAGAACCACAAATAAAAATAATAACAAAAAAACCAATAGAACCTTCAAAAGAAGAAAAAGAAA
TTAACTCTAGAAGTAGAAGTGCAAAATTAAGAGTATGTAGAAAAATATAA

Upstream 100 bases:

>100_bases
ATGAGTGAACTTGGAATATGATTATTTATTATATTTTAACTATTTAGATTTAATCTTAATTATAAAATAAGTGTAAATGC
ATTACATTAGGAGGATACAC

Downstream 100 bases:

>100_bases
ATATATGAGTTTAATGCTTTTAAAAATTTTATATCTAATACTTTAAAAATATAGTTATAAAACAGAAGGAGGCTGAATAA
ATTGGTAATGTTAGAAGCAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MEFKHISVLLEETIDSLNIKEDGVYVDCTLGGGGHSKEILKKLSHKGKLIGIDQDTSAIKAAKERLKDYENVIYVHNNFY
NIDSILEELDIDKVDGIIMDLGVSSYQLDEASRGFSYMKDAPLDMRMNREENLSAYGVINNYEEEELFKILKNYGEEKFS
RKIARFIVEKRTENPIETTGELVEIIRKAIPAKFQREGHPAKRTFQAIRIEVNKELQILNKAIEDSVNRLNKDGRLSIIT
FHSLEDRIVKVKFKELEKPCTCPPSFPICVCGKEPQIKIITKKPIEPSKEEKEINSRSRSAKLRVCRKI

Sequences:

>Translated_309_residues
MEFKHISVLLEETIDSLNIKEDGVYVDCTLGGGGHSKEILKKLSHKGKLIGIDQDTSAIKAAKERLKDYENVIYVHNNFY
NIDSILEELDIDKVDGIIMDLGVSSYQLDEASRGFSYMKDAPLDMRMNREENLSAYGVINNYEEEELFKILKNYGEEKFS
RKIARFIVEKRTENPIETTGELVEIIRKAIPAKFQREGHPAKRTFQAIRIEVNKELQILNKAIEDSVNRLNKDGRLSIIT
FHSLEDRIVKVKFKELEKPCTCPPSFPICVCGKEPQIKIITKKPIEPSKEEKEINSRSRSAKLRVCRKI
>Mature_309_residues
MEFKHISVLLEETIDSLNIKEDGVYVDCTLGGGGHSKEILKKLSHKGKLIGIDQDTSAIKAAKERLKDYENVIYVHNNFY
NIDSILEELDIDKVDGIIMDLGVSSYQLDEASRGFSYMKDAPLDMRMNREENLSAYGVINNYEEEELFKILKNYGEEKFS
RKIARFIVEKRTENPIETTGELVEIIRKAIPAKFQREGHPAKRTFQAIRIEVNKELQILNKAIEDSVNRLNKDGRLSIIT
FHSLEDRIVKVKFKELEKPCTCPPSFPICVCGKEPQIKIITKKPIEPSKEEKEINSRSRSAKLRVCRKI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=34.7181008902077, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=37.7659574468085, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=44.0894568690096, Blast_Score=258, Evalue=3e-70,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=38.6018237082067, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=38.6018237082067, Blast_Score=177, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CLOB1 (A7FTX8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383805.1
- ProteinModelPortal:   A7FTX8
- SMR:   A7FTX8
- STRING:   A7FTX8
- GeneID:   5395391
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1479
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- BioCyc:   CBOT441770:CLB_1479-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35491; Mature: 35491

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKHISVLLEETIDSLNIKEDGVYVDCTLGGGGHSKEILKKLSHKGKLIGIDQDTSAIK
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCHHHHH
AAKERLKDYENVIYVHNNFYNIDSILEELDIDKVDGIIMDLGVSSYQLDEASRGFSYMKD
HHHHHHHHHHCEEEEECCEECHHHHHHHHCCHHHCCEEEECCCCCCCCCHHHCCCHHHCC
APLDMRMNREENLSAYGVINNYEEEELFKILKNYGEEKFSRKIARFIVEKRTENPIETTG
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHH
ELVEIIRKAIPAKFQREGHPAKRTFQAIRIEVNKELQILNKAIEDSVNRLNKDGRLSIIT
HHHHHHHHHCCHHHHHCCCHHHHHHHHHEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEE
FHSLEDRIVKVKFKELEKPCTCPPSFPICVCGKEPQIKIITKKPIEPSKEEKEINSRSRS
EECCCCHHEEEEHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHH
AKLRVCRKI
HHHHHHHCC
>Mature Secondary Structure
MEFKHISVLLEETIDSLNIKEDGVYVDCTLGGGGHSKEILKKLSHKGKLIGIDQDTSAIK
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCHHHHH
AAKERLKDYENVIYVHNNFYNIDSILEELDIDKVDGIIMDLGVSSYQLDEASRGFSYMKD
HHHHHHHHHHCEEEEECCEECHHHHHHHHCCHHHCCEEEECCCCCCCCCHHHCCCHHHCC
APLDMRMNREENLSAYGVINNYEEEELFKILKNYGEEKFSRKIARFIVEKRTENPIETTG
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHH
ELVEIIRKAIPAKFQREGHPAKRTFQAIRIEVNKELQILNKAIEDSVNRLNKDGRLSIIT
HHHHHHHHHCCHHHHHCCCHHHHHHHHHEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEE
FHSLEDRIVKVKFKELEKPCTCPPSFPICVCGKEPQIKIITKKPIEPSKEEKEINSRSRS
EECCCCHHEEEEHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHH
AKLRVCRKI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA